Abstract:
:In test in vitro, four sulfated lycium barbarum polysaccharides (sLBPSs) with different degrees of sulfation (DS), sLBPS0.7, sLBPS1.1, sLBPS1.5 and sLBPS1.9, were added into cultured chicken peripheral lymphocytes and the changes of lymphocytes proliferation were compared by MTT assay taking the non-modified LBPS as control. Two sLBPSs with better efficacy, sLBPS1.5 and sLBPS1.9 were selected. In test in vivo, one hundred 14-day-old chickens were averagely divided into five groups randomly. The chickens except blank control group were vaccinated with Newcastle disease vaccine, repeated vaccination at 28 days old. At the same time of the first vaccination, the chickens in three experimental groups were injected with 0.5 mL of sLBPS1.5, sLBPS1.9 and LBPS at 4 mg mL(-1), in vaccination control group, with 0.5 mL of physiological saline, once a day for three successive days. On days 7, 14, 21 and 28 after the first vaccination, the changes of peripheral lymphocytes proliferation and serum HI antibody titer were determined. The result showed that two sLBPSs could significantly promote lymphocytes proliferation and enhance serum antibody titer. These results indicated that sulfated modification could enhance the immune-enhancing activity of LBPS, which there was a certain relativity with the DS of sulfated polysaccharide. sLBPS1.5 possessed the best efficacy and would be expected as the component drug of a new-type immunopotentiator.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Wang J,Hu Y,Wang D,Liu J,Zhang J,Abula S,Zhao B,Ruan Sdoi
10.1016/j.cellimm.2010.04.001subject
Has Abstractpub_date
2010-01-01 00:00:00pages
219-23issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(10)00096-1journal_volume
263pub_type
杂志文章abstract::Recently, we reported that the CD5 protein can bind to the B cell antigen CD72. So far, no functional evidence has been given for this interaction. We used soluble native CD5 and two anti-CD72 monoclonal antibodies, JT3 and WL225, produced and characterized in our laboratory in order to investigate the role of CD5 in ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0218
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abstract::Medicinal plants are considered immunomodulatory as they display various biological activities. There is no report addressing the anti-inflammatory effects of Daphne gnidium. In this study, we investigated the effects of D. gnidium ethyl acetate (EA) leaf extract on mice immune cell function in vitro. Production of pr...
journal_title:Cellular immunology
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doi:10.1016/j.cellimm.2010.12.002
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90169-2
更新日期:1984-10-15 00:00:00
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1265
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1195
更新日期:1994-07-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1698
更新日期:2000-09-15 00:00:00
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journal_title:Cellular immunology
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更新日期:2012-11-01 00:00:00
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journal_title:Cellular immunology
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doi:10.1016/0008-8749(85)90243-6
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90158-l
更新日期:1992-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90012-7
更新日期:1985-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90268-0
更新日期:1985-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90314-9
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1541
更新日期:1999-08-25 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.01.010
更新日期:2009-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.01.002
更新日期:2006-10-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1481
更新日期:1999-05-25 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.06.001
更新日期:2008-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90322-g
更新日期:1992-11-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90083-9
更新日期:1987-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90171-m
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