Abstract:
:Recently, we reported that the CD5 protein can bind to the B cell antigen CD72. So far, no functional evidence has been given for this interaction. We used soluble native CD5 and two anti-CD72 monoclonal antibodies, JT3 and WL225, produced and characterized in our laboratory in order to investigate the role of CD5 in B cell activation. Neither the CD5 nor the antibodies induced thymidine incorporation when added to resting human B cells, but they produced a two- to five-fold increase in thymidine uptake of B cells activated using immobilized anti-sIgM mAb when compared to the cultures stimulated by anti-sIgM mAb alone. The CD5 protein was effective at concentrations as low as 0.15 microM and its effect could be abolished by preincubation with soluble recombinant CD72 but not by preincubation with control proteins, indicating the specificity of the binding. The two antibodies but not the soluble CD5 produced a costimulatory effect when B cells were stimulated with suboptimal concentrations of anti-CD40 mAb or IL-4. Altogether these data suggest that a costimulatory signal can be delivered to human B cells by CD5/CD72 interaction. The possible role of CD5/CD72 signalling in physiologic humoral responses is discussed here.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Van de Velde H,Thielemans Kdoi
10.1006/cimm.1996.0218subject
Has Abstractpub_date
1996-08-25 00:00:00pages
84-91issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(96)90218-Xjournal_volume
172pub_type
杂志文章abstract::We previously reported the amino acid sequence of the human macrophage chemotactic factor with a molecular weight of 1000 (MCF-1) was WLGREDGSE. In this study we examined the roles of MCF-1 and tumor necrosis factor (TNF) in a guinea pig delayed-type hypersensitivity (DTH) reaction. When macrophage chemotactic activit...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1038
更新日期:1995-04-01 00:00:00
abstract::PICOT (PKC-interacting cousin of thioredoxin) consists of one thioredoxin homology domain in the N-terminal and two tandem PICOT homology domains in the C-terminal. PICOT specifically interacts with protein kinase C theta (PKC-theta) via its thioredoxin homology domain and acts as an important modulator of T cell rece...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.04.005
更新日期:2008-01-01 00:00:00
abstract::Human leucocytes from peripheral blood and tonsil were examined for the presence of the IL-3 receptor using monoclonal antibodies directed to epitopes of the alpha and beta chains of the receptor. We found that the beta chain, common to IL-3, IL-5, and GM-CSF, was either present at low levels or not detected on the ma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0049
更新日期:1996-02-25 00:00:00
abstract::A fibroblastoid cell line TSt-4 was established from fetal thymus tissue of C57BL/6 mice. When fetal thymus (FT) cells or CD4-8- (DN) cells of adult thymuses were cultured on the monolayer of TSt-4, a considerable proportion of lymphocytes expressed CD4 or both CD4 and CD8 within 1 day, and the CD4+CD8- cells were mai...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90299-5
更新日期:1992-07-01 00:00:00
abstract::Cytotoxic T-cell (CTL) cultures were generated from five ovarian cancer patients (OvCTL) and from three breast cancer patients (BrCTL). All CTL lines were T-cell receptor (TcR) alphabeta+ and predominantly CD8+ (73 +/- 13%). These CTL lines preferentially recognized autologous tumor cells in an HLA class I-restricted,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.1055
更新日期:1997-02-01 00:00:00
abstract::Hen egg white lysozyme (HEL)-specific T cell lines and clones were generated from B6 and BDF1 mice. A variety of clonotypes were found among clones generated at an early stage (1 month) whereas fewer clonotypes were detected after several weeks of culture. Furthermore, a bulk line switched from its initial fine peptid...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1088
更新日期:1994-04-01 00:00:00
abstract::Curcumin has commonly been used for the treatment of various allergic diseases. However, its precise anti-allergic rhinitis effect and mechanism remain unknown. In the present study, the effect of curcumin on allergic responses in ovalbumin (OVA)-induced allergic rhinitis mouse was investigated. We explored the effect...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.09.010
更新日期:2015-11-01 00:00:00
abstract::An important subset in regulating antitumor immunity is the maturation and accumulation of intratumor dendritic cells (DCs), inducing potent T cell cytotoxicity. In this study, we explored how the soluble abundant high-mobility group box 1 protein (HMGB1) affected DC activation and retention within lung cancers, and i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.08.011
更新日期:2019-09-01 00:00:00
abstract::An Affymetrix mouse genome array and differential in-gel electrophoresis (DIGE) techniques were used to investigate the pharmacological mechanisms of a mixture of herbs, designated CTCM, a compound of traditional Chinese medicine, for the treatment of increased permeability in mouse intestinal microvascular endothelia...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.06.004
更新日期:2010-01-01 00:00:00
abstract::Endogenous retroviral superantigens such as vSAG-7 are highly stimulatory for T cells through interaction with the T cell receptor on the basis of V beta usage. Priming of adult MMTV 7-negative mice with vSAG-7-expressing cells has been shown to result in peripheral V beta 6/CD4+ T cell activation followed by toleranc...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1164
更新日期:1995-09-01 00:00:00
abstract::Effects of pertussis toxin (PT) on sensitized T-cell populations for delayed-type hypersensitivity (DTH) were examined in mice. DTH was induced by sensitizing mice with ovalbumin (OA) and elicited by injecting OA into the footpad. DTH could be conferred on naive recipient mice by injecting sensitized spleen cells eith...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90249-1
更新日期:1984-05-01 00:00:00
abstract::A monoclonal mouse antibody (HNC-1A3) which defines a subset of human lymphocytes with natural cytotoxic activity was produced and studied. HNC-1A3+ cells represent 12 +/- 3% of peripheral blood mononuclear leukocytes. When sorted out using a fluorescence-activated cell sorter, they consist of 60% small lymphocytes, 3...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90166-1
更新日期:1983-12-01 00:00:00
abstract::T cell hybridomas that are deathless upon TCR crosslinking were generated from lpr mice. The deathless hybridomas (1.4 and 5D5) expressed extremely low Fas even after anti-CD3 activation, whereas activation-induced cell death (AICD) was observed for Fas-expressing hybridomas. The deathless hybridomas were activated to...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0039
更新日期:1996-02-01 00:00:00
abstract::Avirulent clones of Leishmania braziliensis were produced by treatment of parental parasite stock with the mutagen N-methyl-N'-methyl-N'-nitro-N-nitrosoguanidine and selection for growth at 19 degrees C. These clones have a clear preference for infection and growth in vitro at 19 degrees C rather than the normal 28 de...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90081-4
更新日期:1985-08-01 00:00:00
abstract::We compared the effects of calorie restriction (CR) and cyclophosphamide (CTX) on the progression of lupus nephritis and immunological changes in NZB/NZW F1 mice. Ad libitum (AL)/CTX and CR delayed onset of proteinuria and significantly decreased serum levels of anti-dsDNA, anti-histone, and circulating immune complex...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2004.04.001
更新日期:2004-03-01 00:00:00
abstract::The many signals that control the progress of various immune responses to both foreign and self antigens can be divided into no less than three major groups. The first group is the initial positive stimulus, associated with activation events through antigen receptors and their associated proteins. These signals launch...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/0008-8749(90)90174-p
更新日期:1990-10-01 00:00:00
abstract::Idiotope-specific immunoenhancement or suppression was induced in C57BL/6 mice by the injection of physiological amounts (100 ng-10 micrograms) of monoclonal anti-idiotope antibody. As previously described, nanogram doses enhanced idiotope expression while a 10-micrograms dose of anti-idiotope antibody induced the act...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90275-3
更新日期:1986-03-01 00:00:00
abstract::PD-L1 have been identified as the ligand for PD-1, and shown to play a role in the regulation of immune responses. In the present study, we investigated whether overexpressing PD-L1 on islet beta cells could induce negative regulation in primary and primed allogeneic lymphocyte response. pPD-L1-EGFP or pEGFPn1 were tr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.03.007
更新日期:2010-01-01 00:00:00
abstract::The morbidity and mortality of infectious diseases are significantly increased in aged humans. Hence, vaccination has been suggested as a means to reduce or prevent the impact of infections on old individuals. However, it has remained unresolved whether or not standard vaccine adjuvants such as aluminum hydroxide (Alu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00005-9
更新日期:2002-01-01 00:00:00
abstract::This study was undertaken to determine whether bone marrow (BM) cells contain a cell population with the capacity to induce an unresponsiveness of T cells specific to the BM self-H-2 class I antigens in vivo, i.e., veto cell population. Recombinant or congenic mice were infused intravenously with H-2-incompatible BM c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90024-l
更新日期:1990-06-01 00:00:00
abstract::Chronic relapsing experimental allergic encephalomyelitis (CR-EAE) can be adoptively transferred using myelin basic protein (BP)-specific helper T cell lines, and suppressor cells may be important in recovery from EAE. In order to generate suppressor cells, spleen cells obtained from BP-complete Freund's adjuvant (CFA...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90322-i
更新日期:1990-04-01 00:00:00
abstract::Murine Leydig (TM3) and Sertoli (TM4) cell lines were studied as nonprofessional antigen-presenting cells using the antigen model of human choriogonadotropin (hCG alpha/beta) and specific T-cell hybridomas. Both cell lines were treated with IFN-gamma to induce I-A(d) and I-E(d) molecules expression. Only the TM3 cell ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1092
更新日期:1997-04-10 00:00:00
abstract::Chemotactic migration of T lymphocytes and neutrophil granulocytes within a three-dimensional collagen matrix is distinct from spontaneous, matrix-induced migration concerning dynamic parameters and regulatory intracellular signaling. Both spontaneous T lymphocyte locomotion and stromal-cell-derived factor-1 (SDF-1)-i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1605
更新日期:2000-02-01 00:00:00
abstract::We compared TLR responsiveness in PBMC from HIV-1-infected and uninfected individuals using the TLR agonists: TLR7 (3M-001), TLR8 (3M-002), and TLR7/8 (3M-011). Activation and maturation of plasmacytoid dendritic cells (pDC) were measured by evaluating CD86, CD40, and CD83 expression and myeloid dendritic cell (mDC) a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.01.007
更新日期:2007-11-01 00:00:00
abstract::Vitamin D regulates homeostasis, anti-microbial response, and inflammation. The vitamin D receptors are expressed in the macrophages and other immune cells, regulating the transcription of many different genes, including those coding the anti-microbial peptides. One of the most severe complications of the SARS-CoV-2 i...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2020.104259
更新日期:2021-02-01 00:00:00
abstract::As an atypical guanine nucleotide exchange factor (GEF), Dock5 has been extensively studied in cellular functions. However, the role of Dock5 on B-cell immunity still remain elusive. In this study, we generated a Dock5 knockout mouse model to study the effect of Dock5 deficiency on B cell development, differentiation ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.01.001
更新日期:2019-03-01 00:00:00
abstract::Chinese hamster ovary (CHO) cells are commonly used in the generation of transfectants for use in in vitro costimulation assays. However, we have noted that nontransfected CHO cells can themselves provide a low-level B7/CD28 independent costimulatory signal for CD3-mediated murine T cell activation and IL-2 production...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1867
更新日期:2001-11-01 00:00:00
abstract::Prevention of ultraviolet radiation- or chemical carcinogen-induced morphologic transformation and inhibition of tumor-producing transformed cell growth by lymphotoxin and by normal spleen leukocytes were quantitatively compared to define the antineoplastic activity spectra of these natural immune mediators. When Syri...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90372-6
更新日期:1983-03-01 00:00:00
abstract::The nonspecific suppression of immunological responses that is generated within host popliteal lymph nodes upon exposure to syngeneic normal spleen cells has been examined. The suppression, which had previously been described as being capable of preventing initiation of cytotoxic T lymphocytes (CTLs) to hapten-altered...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90069-4
更新日期:1987-03-01 00:00:00
abstract::Cultures of gradient-purified human peripheral blood mononuclear cells (PBMC) have been employed to examine the effects of three bacteria-derived human leukocyte interferon subtypes on certain aspects of in vitro immune responses. The addition of highly purified IFN-alpha 1, -alpha 2, -alpha 2/alpha 1 to PMBC cultures...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90161-2
更新日期:1983-12-01 00:00:00