Abstract:
:PD-L1 have been identified as the ligand for PD-1, and shown to play a role in the regulation of immune responses. In the present study, we investigated whether overexpressing PD-L1 on islet beta cells could induce negative regulation in primary and primed allogeneic lymphocyte response. pPD-L1-EGFP or pEGFPn1 were transfected in NIT-1 cells, for establishment of pPD-L1-EGFP or pEGFPn1 stable transfectants, namely NIT-PD-L1 and NIT-EGFP. In mixed cells reaction, as compared with the controls of NIT-1 or NIT-EGFP, NIT-PD-L1-primed splenocytes showed the lowest proliferative response but severe apoptosis when restimulated with NIT-PD-L1 cells in vitro. Overexpressing PD-L1 on NIT-1 cells could downregulate IFN-gamma but upregulate IL-4 and IL-10 production by the primed lymphocytes. In addition, proliferative response of primary reactive lymphocytes stimulated with NIT-PD-L1 was lower than those lymphocytes restimulated with NIT-1 cells or NIT-EGFP cells. Our data demonstrated that PD-L1 has down-regulative effects on alloimmune responses.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Wen X,Xu G,Liu J,Zhu H,Dai H,Li L,Hong Y,Yang J,Dai W,Ping L,Shen Gdoi
10.1016/j.cellimm.2010.03.007subject
Has Abstractpub_date
2010-01-01 00:00:00pages
122-8issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(10)00069-9journal_volume
263pub_type
杂志文章abstract::We have observed that the CD28 molecule was present on the cell surface of a large fraction of resting CD3- thymocytes (40 to 100%). Interestingly, the majority (greater than 90%) of surface CD3-CD28-cells reacted in the cytoplasm with anti-CD28 (CK248, 9.3) and anti-CD3 epsilon chain mAbs (Leu4, OKT3). Along this lin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90385-o
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abstract::A previous report (G. E. Woloschak and D. Senitzer, submitted for publication) has demonstrated that mitogen-stimulated splenocytes fuse at a much higher frequency than untreated splenocytes as measured by the fusion index (a calculation of the number of nuclei in fused cells vs the total number of nuclei). A measurem...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90059-x
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journal_title:Cellular immunology
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doi:10.1016/0008-8749(86)90275-3
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abstract::We and other investigators have previously demonstrated that mycoplasmas induce macrophage-mediated lysis of tumor cells, but the mechanism responsible for this process had, thus far, not been clarified. We now report that addition of either viable or heat-killed Mycoplasma orale to murine macrophages induces a cytoly...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90012-9
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0020
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abstract::The antimicrobial peptide LL-37 is known to have a potent LPS-neutralizing activity in monocytes and macrophages. Recently, LL-37 in gingival crevicular fluids is suggested to be the major protective factor preventing infection of periodontogenic pathogens. In this study, we tried to address the effect of LL-37 on pro...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.05.005
更新日期:2010-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90180-j
更新日期:1991-03-01 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a curable strategy for the treatment of hematological malignancies and nonmalignant diseases. However, graft-versus-host disease (GVHD) and relapse are still two major causes of morbidity and mortality after allo-HSCT, and both restrict the improvement ...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
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更新日期:2019-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90372-6
更新日期:1983-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1996-05-25 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90169-2
更新日期:1984-10-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104143
更新日期:2020-08-01 00:00:00
abstract::Phagocytosis of pathogens by macrophages is crucial for the successful resolution of inflammation induced by microbial infection. Taurine chloramine (TauCl), an endogenous anti-inflammatory and antioxidative substance, is produced by reaction between taurine and hypochlorous acid by myeloperoxidase activity in neutrop...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.02.003
更新日期:2018-05-01 00:00:00
abstract::It has been previously shown that the activated form of Factor B (Factor Bb) of the alternative pathway of complement activation stimulates monocyte spreading and killing of xenogenic erythrocytes and staphylococci. Factor Bb also stimulates lymphocyte blastogenesis in vitro, and native (uncleaved) Factor B is a major...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90005-x
更新日期:1985-05-01 00:00:00
abstract::Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a chronically intestinal autoimmune disease, the pathological mechanisms of which are not very clear. Wild type p-53 induced phosphatase 1 (Wip1), a serine/threonine protein phosphatase, has been reported to negatively reg...
journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2016-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.005
更新日期:2011-01-01 00:00:00
abstract::Many studies have shown that tumor necrosis factor (TNF) is a potent soluble mediator of immunoregulation and inflammation. Neuropeptide substance P (SP) has been known to exert significant influence on production of certain inflammatory cytokine by immune cells. Immunopathogenic mechanism underlying the effect of neu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.9969
更新日期:1995-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1985-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
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更新日期:1999-05-01 00:00:00
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journal_title:Cellular immunology
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pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1998-08-01 00:00:00
abstract::Poxviruses are a large, complex group of highly successful pathogens that cause disease in humans and other animals. They encode several proteins postulated to be involved in the evasion of host immunity and therefore serve as excellent models for understanding virus-host interaction during the early stages of viral i...
journal_title:Cellular immunology
pub_type: 杂志文章
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