Abstract:
:T cell hybridomas that are deathless upon TCR crosslinking were generated from lpr mice. The deathless hybridomas (1.4 and 5D5) expressed extremely low Fas even after anti-CD3 activation, whereas activation-induced cell death (AICD) was observed for Fas-expressing hybridomas. The deathless hybridomas were activated to produce FasL and IL-2, indicating that the intrinsic defect in Fas expression or up-regulation resulted in AICD blockade. The deathless hybridoma cells expressed longer and stronger FasL cytotoxicity than AICD-sensitive hybridomas. Although deathless, activated 5D5 cells were arrested at the G1/S border. Growth arrest lasted for at least 5 days, but some cells eventually recovered and proliferated. The deathless 5D5 cells were used to demonstrate that AICD includes a fratricidal mechanism that kills AICD-sensitive bystanders. The deathless T cell hybridomas are useful tools for studying T cell activation-dependent functions sensitive to AICD.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Cui H,el-Khatib M,Sherr DH,Ettinger R,Sy MS,Marshak-Rothstein A,Ju STdoi
10.1006/cimm.1996.0039subject
Has Abstractpub_date
1996-02-01 00:00:00pages
302-12issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(96)90039-8journal_volume
167pub_type
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journal_title:Cellular immunology
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