Abstract:
:IL-5 is a pleiotropic cytokine that promotes eosinophil differentiation and survival. While naïve bronchial epithelial cells (BEC) produce low levels of IL-5, the role of BEC-derived IL-5 in allergic airway inflammation is unknown. We now show that BEC, isolated from mice with OVA-induced allergic airway disease (AAD), produced elevated levels of IL-5 mRNA and protein as compared to BEC from naïve mice. To determine the contribution of BEC-derived IL-5 to effector responses in the airways, IL-5 deficient bone marrow chimeric mice were generated in which IL-5 expression was restricted to stromal (e.g. BEC) or hematopoietic cells. When subjected to AAD, IL-5 produced by BECs contributed to mucous metaplasia, airway eosinophilia, and OVA-specific IgA levels. Thus, IL-5 production by BEC can impact the microenvironment of the lung, modifying pathologic and protective immune responses in the airways.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Wu CA,Peluso JJ,Zhu L,Lingenheld EG,Walker ST,Puddington Ldoi
10.1016/j.cellimm.2010.04.008subject
Has Abstractpub_date
2010-01-01 00:00:00pages
32-41issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(10)00116-4journal_volume
264pub_type
杂志文章abstract::The pathogenic antibody response to acetylcholine receptor (AChR) in experimental autoimmune myasthenia gravis (EAMG) is T cell dependent. Therefore, it should be possible to design specific immunotherapeutic approaches to treat EAMG (and human MG) by interfering with AChR-specific helper T cells. Productive T cell ac...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.0012
更新日期:1995-11-01 00:00:00
abstract::We now report two healthy individuals whose T lymphocytes were over 95% positive for CD45RA antigen expression. However, these donors normally expressed both the CD29 high (CD29+) and CD45RO high (CD45RO+) antigens on approximately 40 and 50% of their CD4 cells, respectively. Despite the strong CD45RA expression on th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90089-t
更新日期:1991-10-15 00:00:00
abstract::The regulatory mechanism of guinea pig lymphokines was investigated in regard to differentiation of myeloid cells to macrophages. The Ml-cell line, established from a myeloid leukemia of an SL-strain mouse, was induced to differentiate in vitro into mature macrophages possessing Fc receptors and the ability to phagocy...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90355-6
更新日期:1983-02-15 00:00:00
abstract::A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1279
更新日期:1998-05-01 00:00:00
abstract::The effects of LTs are mediated by GPCRs: cysLTs interact with CYSLTR1, CYSLTR2, or GPR17, and LTB4 acts via BLT1R or BLT2R. Data relating to the presence of these receptors in mature tissue mast cells are not entirely known. By confocal microscopy with image analyses and flow cytometry, we established that native rat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.04.010
更新日期:2017-07-01 00:00:00
abstract::PD-L1 have been identified as the ligand for PD-1, and shown to play a role in the regulation of immune responses. In the present study, we investigated whether overexpressing PD-L1 on islet beta cells could induce negative regulation in primary and primed allogeneic lymphocyte response. pPD-L1-EGFP or pEGFPn1 were tr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.03.007
更新日期:2010-01-01 00:00:00
abstract::The hematopoietically restricted member of the NF-kappaB/Rel family, c-Rel, is essential for B cell survival and proliferation. Here we demonstrate that the production of the interleukins 6, 10, and 15 (IL-6, IL-10, and IL-15) are diminished in c-Rel(-/-) B lymphocytes. In a manner similar to that seen in IL-6(-/-) B ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00513-0
更新日期:2002-05-01 00:00:00
abstract::Murine 4-1BB is an approximately 30-kDa glycoprotein expressed on activated T cells and plays a role in T-cell-mediated proliferative response. To date the majority of work on 4-1BB has been conducted in the mouse. To assess the role of 4-1BB in humans, mAbs were made against the recombinant human (rh) 4-1BB protein. ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0095
更新日期:1996-04-10 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90160-0
更新日期:1983-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1209
更新日期:1993-09-01 00:00:00
abstract::Mice were infected with Plasmodium (P.) yoelii blood-stage parasites. Both the liver and spleen were the sites of inflammation during malarial infection at the beginning of day 7. The major expanding cells were found to be NK1.1(-) intermediate alphabetaTCR (alphabetaTCR(int)) in the liver and spleen, although the pop...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1737
更新日期:2001-01-10 00:00:00
abstract::We have characterized in vivo and in vitro responses of mice to the BALB/c-derived carcinoma, C26. BALB/c mice were highly susceptible, in a dose-dependent fashion, to local tumor development following subcutaneous injection of C26. Other strains of mice, including allogeneic strains and major histocompatibility compl...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90160-3
更新日期:1989-08-01 00:00:00
abstract::The membrane phenotype of T cells involved in delayed hypersensitivity (DH), protective immunity, and suppression of delayed hypersensitivity to herpes simplex virus (HSV) has been determined. T cells from immune lymph nodes transferring DH and antiviral immunity to normal recipients were characterized as Lyt 1+2-. Th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90332-5
更新日期:1983-02-01 00:00:00
abstract::Peripheral blood polymorphonuclear neutrophils (PMN) from mice immunized with Blastomyces dermatitidis and then stimulated locally (intraperitoneally, ip) with B. dermatitidis antigen had enhanced killing of B. dermatitidis in vitro (54.4 +/- 19.49 of inoculum) compared to nonimmune mice (32.7 +/- 8.7%; P less than 0....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90111-0
更新日期:1987-11-01 00:00:00
abstract::In this report, a subset of CD4(+) cells which kills autologous HLA class I positive and HLA class II negative non-small-cell lung cancer (NSCLC) cells is described. Killing was performed both by direct cytolytic mechanisms and by apoptosis. The peculiar characteristic of these effectors was their capability of lysing...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1547
更新日期:1999-09-15 00:00:00
abstract::We studied the effect of vasoactive intestinal peptide (VIP), somatostatin (SOM), and substance P (SP) on IL-4-stimulated human IgE and IgG subclass production. VIP and SOM, but not SP, inhibited IgE production without affecting IgM or IgA production by mononuclear cells (MNC) from nonatopic donors from 10 pM to 10 nM...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90257-p
更新日期:1992-10-15 00:00:00
abstract::Nickel (Ni) can cause delayed-type hypersensitivity reactions, which are thought to be mediated by the accumulation of T cells into inflamed skin. Accumulated T cells at the developmental stages in metal allergy are poorly characterized because a suitable animal model has not been established. To investigate the accum...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.07.010
更新日期:2013-07-01 00:00:00
abstract::A variety of arachidonic acid metabolites possess the ability to modulate immune cell function. Various inhibitors of arachidonic acid metabolism were compared with regard to their effects on T-suppressor (Ts) cell function. Using staphylococcal enterotoxin B (SEB) to activate Lyt-2+ Ts cells, it was shown that indome...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90211-3
更新日期:1983-10-01 00:00:00
abstract::We describe here a culture system for studying the development, in vitro, of antigen-specific CD4 T cells from unprimed mice. T cells from young mice are initially exposed to antigen, such as pigeon cytochrome C or keyhole limpet hemocyanin, in the presence of adherent accessory cells and then allowed to proliferate i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1134
更新日期:1997-06-15 00:00:00
abstract::Polyinosinic-polycytidilic acid (poly (I:C], a synthetic analog of viral double-stranded RNA (dsRNA), activates natural killer (NK) cells and inhibits induction or promotes termination of the primary IgM response in vivo. Suppression of responses was reproduced in vivo by interferons (IFN) which activate NK cells and ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90287-x
更新日期:1986-04-01 00:00:00
abstract::Human leucocytes from peripheral blood and tonsil were examined for the presence of the IL-3 receptor using monoclonal antibodies directed to epitopes of the alpha and beta chains of the receptor. We found that the beta chain, common to IL-3, IL-5, and GM-CSF, was either present at low levels or not detected on the ma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0049
更新日期:1996-02-25 00:00:00
abstract::Antigen processing in B cells is initiated by antigen binding to the surface B cell antigen receptor (BCR). The BCR is a signaling receptor which also functions to endocytose bound antigen for subsequent intracellular processing and presentation with class II molecules. Previously, using subcellular fractionation, we ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1264
更新日期:1998-02-25 00:00:00
abstract::Permanent graft acceptance was induced with cyclosporine A in a low responder rat strain combination PVG (RT1(c)) to DA (RT1(av1)) of cardiac transplants. Challenge (alloimmunization) with donor and third-party WF (RT1(u)) cells was performed and followed by the analysis of alloreactive cytotoxicity. The cytotoxic T l...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1353
更新日期:1998-10-10 00:00:00
abstract::We describe here a novel real-time cell tracking system which can measure cell migration routes under cell culture condition. This system includes a mini incubator which controls temperature and CO(2) gas flow and a PDMS (polydimethylsiloxane) chip for chemotaxis measurement. The main differences from previous ones ar...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.07.001
更新日期:2010-01-01 00:00:00
abstract::Induction of specific antitumor cytotoxic T cell responses was studied in BALB/c mice bearing tumors transfected with a mutant human p53 minigene. We observed that mice were resistant to the induction of peptide-specific CTL as early as 5 days after challenge with a minimal lethal dose of tumor cells, and the cell typ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0139
更新日期:1996-05-25 00:00:00
abstract::A series of Lyt 2+, trinitrophenyl (TNP)-specific T-cell lines are shown to lyse 51Cr-labeled target cells in an antigen-specific, H-2-restricted fashion in a 4-hr assay. These cells also produce lymphotoxin, in addition to other factors, upon stimulation with TNP-haptenated syngeneic splenocytes. A technique for intr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90389-2
更新日期:1985-06-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.12.002
更新日期:2010-01-01 00:00:00
abstract::Stimulation via the CD3/TCR molecular complex induces proliferation of resting T cells, but triggers programmed cell death (apoptosis) in immature thymocytes and preactivated mature T cells. Activation-induced cell death (AICD) triggered by anti-CD3/TCR mAb or by staphylococcus enterotoxin superantigen is associated w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.9979
更新日期:1995-12-01 00:00:00
abstract::Positive selection of T lymphocytes expressing self. MHC-restricted T cell receptors is mediated by MHC molecules expressed on thymic stroma. Among the more controversial aspects of this process is the relative role of MHC molecules on epithelial versus bone marrow-derived stromal elements (BM-APC). For CD4+ T cells, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0175
更新日期:1996-07-10 00:00:00
abstract::The effects of oral administration of methylated bovine serum albumin (mBSA) on antigen-induced arthritis (AIA) in rats that was induced by immunization with mBSA followed by the intraarticular injection of the same antigen were investigated. When mBSA was orally given to animals before immunization with the antigen, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1098
更新日期:1995-06-01 00:00:00