Abstract:
:Previous studies established that low in vitro temperatures (27 degrees C, termed nonpermissive) suppressed murine primary thymus-dependent (TD), but not primary thymus-independent (TI) or secondary TD, antibody responses. Suppression was rescued by the addition of oleic acid (18:1), recombinant interleukin (rIL)-2 and/or rIL-4, but not rIL-1. These observations suggested that low temperatures suppress the functions of virgin T cells (Tv) but not those of memory T cells (Tm), B cells, or accessory cells and that hypothetically 18:1 may rescue suppressed responses by altering the membranes of Tv cells, allowing them to proliferate and subsequently secrete interleukins. In this study negatively selected Tm and Tv cells were stimulated with various mitogens at 37 or 27 degrees C. The results indicated that proliferation was suppressed at 27 degrees C to all mitogens tested. The subsequent addition of 18:1 induced proliferative responses at 27 degrees C to both concanavalin A (Con A) and a combination of 12-O-tetradecanoylphorbol 13-acetate (TPA) and calcium ionophore (A23187), but not to phytohemagglutinin-P (PHA). Both Tm and Tv cells showed significant secretion of IL-2 and expression of IL-2 receptor (IL-2R) at 27 degrees C in response to all mitogens, irrespective of proliferation, and the subsequent addition of 18:1 caused little or no change in the levels of IL-2 secretion or IL-2R expression. These findings indicate that suppression of Tm and Tv cell proliferative responses occurs irrespective of IL-2R expression and, unlike antibody production, of IL-2 secretion. Furthermore, it appears that 18:1 can synergistically act with Con A or TPA/A23187, but not PHA, in activating Tm and Tv cells to induce proliferation at 27 degrees C. These findings suggest differences in signal transduction mechanisms between these various mitogens.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Wooten RM,Clem LW,Bly JEdoi
10.1006/cimm.1993.1265subject
Has Abstractpub_date
1993-11-01 00:00:00pages
35-48issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(83)71265-7journal_volume
152pub_type
杂志文章abstract::One of the auto-antigens aberrantly targeted in Multiple sclerosis is myelin basic protein (MBP). In this study, chronic progressive multiple sclerosis (CPMS) patients receiving the experimental drug MBP8298, on a compassionate care trial, were examined before and after high dose peptide treatment for their circulatin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.11.004
更新日期:2012-11-01 00:00:00
abstract::A new mechanism for regulation of delayed-type hypersensitivity (DTH) was investigated. The subcutaneous injection without adjuvant of syngeneic epidermal Langerhans' cells (LC) pulsed with keyhole limpet hemocyanin (KLH) into BALB/c mice gave rise to DTH upon challenge of the ear with the same antigen. When such cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1279
更新日期:1993-11-01 00:00:00
abstract::AK-5 is a spontaneously regressing rat histiocytoma and the tumor regression is mediated by CD8+ NK cells. We have investigated the functional status of effector cells mediating complete remission of the tumor. The effector-target interaction takes place with the help of antibody to AK-5 and the mechanism of lysis inv...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1056
更新日期:1994-03-01 00:00:00
abstract::Tumor necrosis factor (TNF) is a 17-kDa protein produced by endotoxin-stimulated macrophages. We have demonstrated that recombinant human TNF activates human macrophages to kill intracellular bacteria of the Mycobacterium avium complex (MAC) in a dose-related manner. TNF also primed macrophages to produce superoxide a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90243-i
更新日期:1992-10-15 00:00:00
abstract::The effects of oral administration of ovalbumin (OVA) on acute OVA-induced arthritis (OIA) in rats, which is mediated by Arthus reaction to the antigen in the joint space, were investigated. The oral administration of OVA before immunization with OVA significantly suppressed the development of acute OIA in a dose-depe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.1049
更新日期:1997-01-10 00:00:00
abstract::The functional specificity of three types of macrophage chemotactic factors ( MCFs ), -a, -b, and -c, from purified protein derivative of tuberculin (PPD)-induced delayed hypersensitivity reaction (DHR) skin sites on guinea pigs, was analyzed using macrophage cell line cells, M1, established from myeloid leukemia cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90354-x
更新日期:1984-06-01 00:00:00
abstract::We describe here a novel real-time cell tracking system which can measure cell migration routes under cell culture condition. This system includes a mini incubator which controls temperature and CO(2) gas flow and a PDMS (polydimethylsiloxane) chip for chemotaxis measurement. The main differences from previous ones ar...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.07.001
更新日期:2010-01-01 00:00:00
abstract::We tested the effects of salen manganese (Salen-Mn) complexes, which are scavengers of reactive oxygen species exhibiting superoxide dismutase and catalase activities on the rejection of and alloresponse to fully allogeneic skin grafts in mice. We showed that pre-transplant treatment of C57Bl/6 donor skin or of BALB/c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.06.009
更新日期:2006-06-01 00:00:00
abstract::T helper cells reactive to myelin basic protein are clearly implicated in the pathogenesis of murine EAE. We have developed a T cell line, BML-1 that (1) is reactive to the encephalitogenic amino terminal nonapeptide (1-9NAC) of MBP, (2) is I-Au restricted, and (3) induces relapsing EAE in B10.PL (H-2u) mice. Measurem...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90117-2
更新日期:1989-11-01 00:00:00
abstract::Gene products coded for within the HLA complex play an important role in the control of immune responses. Class I antigens, coded for by the HLA-A, B, and C loci, are expressed by virtually all mononuclear blood cells. Class II antigens, coded for by the DR, DQ, and DP loci, have a more limited tissue distribution. Th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90050-x
更新日期:1986-07-01 00:00:00
abstract::The complement receptor 2 (CR2; CD21), a 145,000 MW glycoprotein, has been useful as a marker of B lymphocyte maturation. It is expressed on the 1:13 monoclonal, EBV-transformed, B cell line which produces TNP-specific IgM-kappa and displays an in vitro capacity for differentiation. The line expresses the CD20+CD21+ p...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90093-7
更新日期:1990-02-01 00:00:00
abstract::Previous investigations have demonstrated a strain-associated differential susceptibility to herpes simplex virus-1 (HSV-1) infection in mice; C57BL/6 mice are more resistant to infection than BALB/c mice. Despite considerable research dealing with the immune response to HSV-1, relatively little attention has been pai...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1246
更新日期:1994-09-01 00:00:00
abstract::Conalbumin (CA)-specific type 2 helper T cell (Th2) clone, D10G4.1 (D10) produces IL4 when stimulated with varying doses of TNP-CA in the presence of mitomycin C-treated C3H spleen cells or purified B cells as antigen-presenting cells (APC). The production of IL4 was assessed by bioassay and by expression of IL4 mRNA....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90333-k
更新日期:1992-12-01 00:00:00
abstract::Multiple previous studies have demonstrated significant alterations of immunologic parameters associated with mouse hepatitis virus (MHV) infection, but effects of the virus on mucosal lymphoid cells have not been examined. Coincident with a natural outbreak of MHV at our institution, we noted alterations in immunoglo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90295-4
更新日期:1987-10-01 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) play a major role in modulating immune response, but only a few reports focused on MDSCs in the liver of sepsis states. Here, we investigated the changes in MDSCs in liver of the cecal ligation and puncture (CLP) mice. The results of flow cytometry showed that MDSCs accumulate ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.08.005
更新日期:2012-09-01 00:00:00
abstract::Previously, we reported that human B lymphocytes can be stimulated by trinitrophenylated Brucella abortus (TNP-Ba) in vitro to generate T-independent (TI) hapten specific plaque-forming cells (PFC). Furthermore we showed that addition of Con A on Day 3 of culture enhanced the anti-TNP response. In this report the char...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90245-4
更新日期:1984-05-01 00:00:00
abstract::Treatment of human blood mononuclear cells with nanomolar concentrations of phorbol 12,13-dibutyrate (P(Bu)2) induced their aggregation. The phenomenon was seen within a few minutes and reached its maximum manifestation after 20 min. At this time, 20-30% of unfractionated and nylon wool-passed mononuclear leukocytes w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90313-1
更新日期:1983-01-01 00:00:00
abstract::Sensitized T lymphocytes can mediate potent antitumor effects when transferred to tumor-bearing animals. Employing the MCA 105 and MCA 106 sarcomas, we were able to generate antitumor effector cells by immunization of syngeneic mice with tumor cells admixed with Corynebacterium parvum. These immune splenocytes could b...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90209-8
更新日期:1989-05-01 00:00:00
abstract::The role of surface aminopeptidases (APs), enzymes that cleave amino-terminal residues from polypeptide chains, in the development of fetal thymocytes was studied using a murine fetal thymic organ culture (FTOC) model. FTOC AP activity was demonstrable for various amino acid-p-nitroanilide substrates, and specific inh...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1099
更新日期:1997-04-10 00:00:00
abstract::In this study a panel of monoclonal antibodies was used to investigate the kinetics of the appearance of activation-linked surface determinants as well as cytoplasmic and nuclear determinants in human T cells following lectin stimulation. Well known activation markers, such as Ia/DR, transferrin receptor, IL-2 recepto...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90197-2
更新日期:1985-02-01 00:00:00
abstract::Immunity to Schistosoma mansoni is regulated by an auto-anti-idiotypic network. The T cells in this network interact through soluble regulatory factors. These studies describe a soluble suppressor-inducer factor (TsiF) derived from TCR+ suppressor-inducer clone Q37. Q37 is the fusion product of CD4+ T cells derived fr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1144
更新日期:1993-06-01 00:00:00
abstract::The cellular requirements for stimulating primed alloreactive T cells have been investigated. In vitro-primed secondary alloreactive cells, long-term lines, and Ly 1+2- noncytolytic clones which reacted with allo-H-2K, D, or Mls (M locus) antigens were tested. The data indicated that a specialized antigen-presenting c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90032-2
更新日期:1985-03-01 00:00:00
abstract::In the present study, we characterized in monocytes the rise in [Ca(2+)](i) evoked by monoclonal antibodies (mAbs) to aminopeptidase N (APN)/CD13, showing a two-phase calcium increase with a small-belled [Ca(2+)](i) rise due to the release of calcium from intracellular stores and a more sustained plateau due to the in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1629
更新日期:2000-04-10 00:00:00
abstract::At concentrations as low as 10(-7) M, the cardiotonic glycosteroid ouabain, a specific inhibitor of the membrane Na+, K+-ATPase, is known to inhibit in vitro human lymphocyte proliferation produced in mixed lymphocyte cultures or induced by various stimulating agents (PHA, Con A, PWM, soluble antigens), while mouse ly...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90314-9
更新日期:1984-02-01 00:00:00
abstract::We recently reported that a cloned murine cell line with "natural killer (NK)-like" cytolytic function and prominent cytoplasmic granules also expressed large numbers of plasma membrane receptors (Fc epsilon R) which bound mouse immunoglobulin E (IgE) with high affinity (S.J. Galli et al., 1982, Nature (London) 298, 2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90353-3
更新日期:1985-11-01 00:00:00
abstract::Theiler's murine encephalomyelitis virus-induced demyelinating disease (TMEV-IDD), a model for multiple sclerosis, is a chronic T cell-mediated disease. Development of clinical symptoms in susceptible mouse strains generally correlates with TMEV-specific delayed-type hypersensitivity (DTH) responses. These responses, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1303
更新日期:1993-12-01 00:00:00
abstract::Autoimmune regulator (Aire) is a transcriptional activator that regulates the ectopic expression of many tissue-restricted antigens in medullary thymic epithelial cells, and that has an important role in the negative selection of autoreactive T cells. However, the roles of Aire expression in peripheral lymphoid tissue...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.05.002
更新日期:2011-01-01 00:00:00
abstract::The expression of HLA Class II antigens by resting and phytohemagglutinin (PHA)-activated T cells and their functional properties in autologous mixed-lymphocyte reactions (MLR) were investigated in patients with chronic active hepatitis, with alcoholic cirrhosis, and with primary biliary cirrhosis. In all groups of pa...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90341-2
更新日期:1986-10-01 00:00:00
abstract::CD40 and its ligand (CD40L) regulate several cellular functions, including T and B-cell activation. The soluble form of CD40 (sCD40) antagonizes CD40/CD40L interaction. Patients undergoing hemodialysis (HD) present elevated sCD40 serum levels, which underlying molecular mechanisms are unknown. We studied sCD40 serum a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.06.007
更新日期:2012-07-01 00:00:00
abstract::PHA stimulation of both freshly isolated and IL-2-dependent cultured T cells induced a rapid rise in intracytoplasmic calcium concentration. Chelation of extracellular calcium with EGTA resulted in a failure of PHA to induce a rise in intracytoplasmic calcium, resulting in the fresh T cells in an inhibition of IL-2 pr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90060-3
更新日期:1988-01-01 00:00:00