Abstract:
:Expression of certain autologous lymphocyte-activating antigenic determinants on the developing embryo is known to provide a stimulus for maternal anti-fetal autoproliferative responses. If left unregulated these responses could exert negative influences on the reproductive process by converting to autoaggressive forms of immune reactivity. In normal circumstances, immunological reactions of this nature are therefore likely to be under the control of pregnancy-associated immunoregulatory elements found within the maternal/fetal environment. In the present investigation we describe a naturally occurring splenic inhibitory cell type devoid of conventional T, B, and macrophage surface markers associated with syngeneic murine pregnancy that is capable of exerting potent immunosuppressive effects on an in vitro expression of fetal/newborn T cell autoreactivity, namely the autologous mixed lymphocyte reaction (AMLR). Maternal spleen cells inhibitory for AMLR were found to be highly resistant to cytotoxic pretreatment with a panel of conventional antisera directed against T cell-specific antigenic determinants. The non-T nature of the natural splenic suppressor cell was further indicated by experiments showing that purified spleen T cells had no inhibitory activity. Pregnancy spleen cell populations that were effectively depleted of macrophages retained full ability to inhibit AMLR. Maternal suppressor activity could be localized to the spleen cell population bearing receptors for the B cell-specific lectin, soybean agglutinin (SBA). A panel of monoclonal antibodies prepared against enriched populations of suppressor cells was screened and selected for specific reactivity using an ELISA against glutaraldehyde-fixed SBA+ spleen cell subpopulations from pregnant versus virgin animals. Several of the monoclonals developed against suppressor-enriched spleen cell populations from isopregnant as well as allopregnant animals were effective in reducing or eliminating suppressor cell activity following cytotoxic pretreatment in the presence of complement. The novel set of anti-suppressor monoclonal antibodies described here should prove useful in furthering the isolation and characterization of pregnancy-associated suppressor cells and in determining their relationship to natural suppressor cell populations described in other systems.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Hoskin DW,Gronvik KO,Hooper DC,Reilly BD,Murgita RAdoi
10.1016/0008-8749(89)90173-1subject
Has Abstractpub_date
1989-04-15 00:00:00pages
42-60issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(89)90173-1journal_volume
120pub_type
杂志文章abstract::Within lymphopenic recipients, naïve T cells undergo proliferation that is induced by homeostatic mechanisms. Earlier studies have demonstrated that commensal antigens play a key role in inducing the proliferation. However, a relative contribution of endogenous self antigens in this process has not been formally inves...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.11.002
更新日期:2012-01-01 00:00:00
abstract::The phenotype, T-cell antigen receptor (TCR) usage and specificity of intestinal intraepithelial lymphocytes (IEL), and hybridomas derived from IEL have been examined. In young conventionally reared mice, approximately 50% of the IEL express TCR alpha beta and 50% express TCR gamma delta, although there is considerabl...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1293
更新日期:1993-12-01 00:00:00
abstract::Although it is known that IGF-1 increases T lymphocyte proliferation, the regulation of its receptor expression during the process has not been defined. Consequently the regulation of IGF-1R expression by IGF-1 and the activation events in human blood T lymphocytes and the Jurkat T cell line were now investigated. IGF...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1237
更新日期:1998-02-01 00:00:00
abstract::Our earlier studies revealed that a rapid and progressive loss of splenic NK activity in mice during the development of a number of transplanted tumors as well as of spontaneous tumors was due to an inactivation of natural killer (NK) lineage cells rather than to their disappearance. The mechanism of this inactivation...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90133-9
更新日期:1985-07-01 00:00:00
abstract::Monoclonal-nonspecific suppressor factor (MNSF) is a lymphokine derived from murine T cell hybridoma. The target tissues are both LPS-stimulated B cells and Con A-stimulated T cells. Since the action of MNSF may be mediated by its binding to specific cell surface receptors, we characterized the mode of this binding. T...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90271-r
更新日期:1990-10-15 00:00:00
abstract::Two signals are required for the in vitro activation of Lyt2+ T suppressor cells (Ts) from mice tolerized with 2,4-dinitrobenzene sulfonate (DNBS) to produce soluble suppressor factors (SSF) which suppress the transfer of contact sensitivity to dinitrofluorobenzene (DNFB). Recognition of DNP/class I MHC (signal one) s...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90187-g
更新日期:1991-03-01 00:00:00
abstract::T cell hybridomas that are deathless upon TCR crosslinking were generated from lpr mice. The deathless hybridomas (1.4 and 5D5) expressed extremely low Fas even after anti-CD3 activation, whereas activation-induced cell death (AICD) was observed for Fas-expressing hybridomas. The deathless hybridomas were activated to...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0039
更新日期:1996-02-01 00:00:00
abstract::Large suppressive macrophages (LSM) were induced by restimulating spleen cells from rats with experimental autoimmune myasthenia gravis (EAMG) in vitro, with the autoantigen acetylcholine receptor (AChR) in the presence of cyclosporine A. LSM, purified from these cultures, are extremely potent suppressors of AChR-stim...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1445
更新日期:1999-04-10 00:00:00
abstract::The reactivity of human cord blood lymphocytes was assessed against a panel of monoclonal antibodies (MoAb). The mean proportion of OKT3+ cells (pan-T) was significantly lower in cord blood (52 +/- 13.8%; mean +/- SD) compared with that of adult blood (75 +/- 8.9%) and paralleled well with the E-rosette-forming capaci...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90209-0
更新日期:1984-11-01 00:00:00
abstract::Inhibition of DPP IV has been shown to abrogate the stress-related increase in murine abortions and a concomitant increase in gamma-interferon. The aim of the present study was to investigate a potential impact of the DPP IV inhibitor Isoleucine Cyanopyrrolidide on the expression of surface antigens involved in T-cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00028-5
更新日期:2002-12-01 00:00:00
abstract::Spontaneously occurring lymphomas in SJL mice have many pathological features similar to Hodgkin's lymphoma in humans. The malignant growth of the tumor cells is dependent on the support of host FoxP3(+)CD4(+) regulatory T cells (Tregs). In this study, we report that the ablation of golli protein, a negative regulator...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.01.006
更新日期:2016-04-01 00:00:00
abstract::Inflammatory leukocytes of DA-to-WF rat renal allografts displayed significant cytolytic activity to natural killer (NK) target cells on Day 2 after transplantation. The NK activity, which was associated with large granular lymphocytes in discontinuous Percoll gradients, peaked on Day 4 and disappeared rapidly thereaf...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90342-3
更新日期:1984-12-01 00:00:00
abstract::Functionally distinct subpopulations within the CD4+ subset of T lymphocytes have been described in man, rat, and mouse. In the rat different functions have been assigned to CD45R+ and CD45R- T helper cells. The CD45R+ in contrast to the CD45R- T helper cells have been reported to produce IL-2 and to proliferate well ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90247-5
更新日期:1989-04-01 00:00:00
abstract::The recombinant retrovirus J2, which contains the v-raf/mil and v-myc oncogenes, was used to immortalize mouse splenic macrophages that had been cloned in soft agar. When added to freshly harvested colonies, J2 failed to yield cell lines but it immortalized up to 30% of the clones if they had been maintained for at le...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90236-5
更新日期:1988-10-15 00:00:00
abstract::Antigen-specific T-helper factor (ThF) of CBA (H-2k) origin in the picryl (TNP) contact sensitivity system (Mr 60-70 kDa) was reduced with dithiothreitol under mild conditions. Affinity chromatography on antigen yielded an antigen-binding chain (Mr 20-30 kDa) and an antigen-nonbinding chain (Mr 40-50 kDa). Both chains...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90037-8
更新日期:1988-05-01 00:00:00
abstract::We previously demonstrated that injection of a high dose (4 X 10(9] of sheep erythrocytes (SRBC) into C57BL/6 mice results in the generation of splenic B cells (plastic nonadherent, Thy-1- and Ig+) which, when transferred to normal syngeneic recipients, subsequently induce antigen-specific suppressor T cells to suppre...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90242-9
更新日期:1984-01-01 00:00:00
abstract::In the search for its role in inflammatory joint diseases, soluble HLA-DR (sHLA-DR) was quantitated in 72 synovial fluids (SF) by a newly established immunoenzyme assay. Unlike other soluble receptors which accumulated only moderately (sCD25, sCD4) or negligibly (sHLA class I, sCD8) in the SF, SF sHLA-DR levels exceed...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1729
更新日期:2000-12-15 00:00:00
abstract::A variant nonkiller (K-) T-cell clone derived from the MHC nonrestricted killer (K+) cell line TALL-103/2 (CD3/TCR gamma delta +) was studied to determine whether its lytic defects were at the tumor-binding or post-binding level. The two TALL-103/2 clones were found to display similar mRNA expression of TCR/CD3 comple...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1212
更新日期:1995-10-15 00:00:00
abstract::Controversies remain about NK cells direct responsiveness to Toll-like receptor (TLR) agonists or dependence on macrophages. In a melanoma lung metastasis model, aerosolized TLR9 and TLR3 agonists have been reported to induce antitumor immunity through NK cells activation. In the current study, we demonstrated that in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.01.004
更新日期:2017-03-01 00:00:00
abstract::IL-2 was previously shown to induce cytotoxic effectors with a broad spectrum of target specificities in thymus and spleen cell cultures. This study was designed to show whether T cells activated by H-2 allogeneic cells in MLC or by syngeneic tumor cells in MLTC are also potential targets for these cytotoxic effectors...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90124-x
更新日期:1989-12-01 00:00:00
abstract::The role of macrophages (M phi) and that of splenic dendritic cells (DC) in the allogeneic mixed leukocyte reaction (MLR) in the mouse have been investigated. In contrast with the high stimulatory capacity of DC, we obtained no evidence in favor of the competence of M phi, whether Ia + or Ia-, as an autonomous stimula...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90169-2
更新日期:1984-10-15 00:00:00
abstract::Previous studies have shown that supernatants from concanavalin A-stimulated human peripheral blood mononuclear cells and isolated Leu-2 suppressor/cytotoxic T cells are chemotactic for Leu-3 helper/inducer T cells. The current study shows that lymphocyte chemotactic factor (LCF) is also produced following antigen (te...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90375-8
更新日期:1986-01-01 00:00:00
abstract::This study addresses the nature of the pathogenic effector T cell in experimental autoimmune uveoretinitis and the effect of different cytokines on these cells in vitro. Lymph node cells of B10.RIII mice immunized with the uveitogenic peptide 161-180 of interphotoreceptor retinoid binding protein were cultured with th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1121
更新日期:1997-05-25 00:00:00
abstract::Following interaction of the random polymer (Glu60,Phe40)n (GPhe) with antigen-presenting accessory cells (APC), unusual costimulatory activities were noted in several murine T cell systems. When GPhe, in contrast with other random copolymers (GT,GL), was added during "inhibition" and T cell "repertoire" studies as a ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90198-z
更新日期:1990-08-01 00:00:00
abstract::Various anti-Fc gamma RIII (CD16) monoclonal antibodies (mAbs) are shown here to have positive or negative modulatory effects on human NK cells. Thus, 3G8 mAb (IgG1) triggered a dose-dependent augmentation of NK activity in 67% (23/34) of individuals tested, who were designated as responders. All four IgG1 anti-CD16 m...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1113
更新日期:1995-07-01 00:00:00
abstract::Blockade of the immune cell checkpoint inhibitors programmed death 1 (PD-1) and programmed death-ligand 1 (PD-L1) has become a powerful tool in cancer treatment, which is effective across various solid cancer types and hematologic malignancies. Our previous studies showed that by reducing immune tolerance, clustered r...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.09.003
更新日期:2017-10-01 00:00:00
abstract::Micromolar amounts of SV-IV, one of the major proteins secreted from the rat seminal vesicle epithelium, induce in vitro a marked release of a variety of cytokines (interferon-gamma, tumor necrosis factor-alpha, interleukin 6, and granulocyte-monocyte colony-stimulating factor) from human resting peripheral blood mono...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0061
更新日期:1996-03-15 00:00:00
abstract::Basic Immunology has had only two significant public priority disputes. The first began in the late 1650s and concerned the recognition of the peripheral network of vessels which collects lymph throughout the body. The publication of this major anatomical discovery prompted a priority feud discussed in a previous pape...
journal_title:Cellular immunology
pub_type: 历史文章,杂志文章
doi:10.1016/j.cellimm.2007.02.015
更新日期:2007-01-01 00:00:00
abstract::Purine nucleoside phosphorylase (PNP) is a purine salvage pathway enzyme which we have found to be 8-10 times more active (per cell) in human peripheral blood null lymphocytes than in T lymphocytes. To test the hypothesis that null cells are, in part, pre-T lymphocytes we have defined an in vitro system for null cell ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90288-5
更新日期:1983-06-01 00:00:00
abstract::Tumors harbor a population of cancer stem cells (CSCs) which can drive tumor progression and therapeutical resistance. Nature killer (NK) cells are best known for their ability to directly recognize and kill malignant cells. However, the susceptibility of cancer stem cells to NK cells is not fully understood. Here we ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.11.009
更新日期:2016-02-01 00:00:00