Abstract:
:Controversies remain about NK cells direct responsiveness to Toll-like receptor (TLR) agonists or dependence on macrophages. In a melanoma lung metastasis model, aerosolized TLR9 and TLR3 agonists have been reported to induce antitumor immunity through NK cells activation. In the current study, we demonstrated that in vitro TLR9/TLR3 stimulation induced IFN-γ secretion by NK cells, but an increase in their cytotoxicity was detected only after NK cells co-culture with in vitro TLR9/TLR3 agonists pretreated alveolar macrophages. Alveolar macrophages from melanoma lung metastases-bearing mice, treated with aerosolized TLR agonists, also promoted NK cell cytotoxicity. Activated NK cells from lungs of melanoma metastases-bearing mice that were given aerosolized TLR9/TLR3 agonists were able to polarize naive alveolar macrophages toward a M1-like phenotype. Our results demonstrate that activation of NK cells in the lung after TLR engagement is mediated by alveolar macrophages and that activated NK cells shape macrophage behavior.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Sommariva M,Le Noci V,Storti C,Bianchi F,Tagliabue E,Balsari A,Sfondrini Ldoi
10.1016/j.cellimm.2017.01.004subject
Has Abstractpub_date
2017-03-01 00:00:00pages
52-58eissn
0008-8749issn
1090-2163pii
S0008-8749(17)30004-7journal_volume
313pub_type
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