Abstract:
:We have compared the interaction of Mycobacterium tuberculosis with the human macrophage-like cell line THP-1 and with human monocyte-derived macrophages (MDM). The association of M. tuberculosis with THP-1 and MDM was comparable in both the presence and the absence of serum. For both cells, serum-mediated binding was much greater than nonopsonic binding and was mediated by a heat-labile serum component. Nonopsonic binding of M. tuberculosis to both cells could be inhibited by antibodies recognizing CD11b and by mannan and glucan. Intracellular M. tuberculosis grew progressively in infected MDM and THP-1 cells. Treatment of the infected MDM and THP-1 cells with the anti-mycobacterial isoniazid resulted in the rapid killing of the intracellular mycobacteria. Differentiated, adherent THP-1 cells bound IgG and complement-coated particles at levels similar to those of MDM. However, binding of zymosan by THP-1 cells was significantly lower than that seen for MDM.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Stokes RW,Doxsee Ddoi
10.1006/cimm.1999.1554subject
Has Abstractpub_date
1999-10-10 00:00:00pages
1-9issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(99)91554-Xjournal_volume
197pub_type
杂志文章abstract::Cells from the spleen, lymph nodes, and peritoneum of DBA/2 mice bearing a subcutaneous tumor mediate nonspecific suppression of an in vitro antibody response to sheep red blood cells (SRBC) when cocultured with a normal T-cell subset(s). The spleen cells from the tumor-bearing mouse required for the suppression bear ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90047-3
更新日期:1983-07-01 00:00:00
abstract::The nuclear receptor Small Heterodimer Partner (SHP) is a transcriptional target and inhibitor of Liver Receptor Homolog 1 (LRH-1), the transcriptional regulator of intestinal glucocorticoid (GC) synthesis. The role of SHP in the regulation of intestinal GC synthesis and its impact on T cell-mediated anti-viral immune...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.08.009
更新日期:2018-12-01 00:00:00
abstract::Immunocytochemical staining procedures were combined with computer-aided image analysis to quantitate the relative intracellular production of tumor necrosis factor-alpha (TNF) within individual macrophages. Optimal conditions for time and methods for the activation of TNF production, fixation of cells for optimal imm...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90278-y
更新日期:1990-10-15 00:00:00
abstract::Inflammatory leukocytes of DA-to-WF rat renal allografts displayed significant cytolytic activity to natural killer (NK) target cells on Day 2 after transplantation. The NK activity, which was associated with large granular lymphocytes in discontinuous Percoll gradients, peaked on Day 4 and disappeared rapidly thereaf...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90342-3
更新日期:1984-12-01 00:00:00
abstract::Although it is well known that the CD3/T-cell receptor (TCR) complex modulates from the surface of T cells upon exposure to monoclonal antibodies (mAb) directed against it, the fate of bound mAb has not been yet elucidated. We therefore perform direct binding experiments of 125I-labeled mAb against CD3 or TCR to inves...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90209-2
更新日期:1988-10-01 00:00:00
abstract::Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (alpha 1 and alpha 2) of H-2Kb and the carboxy-terminal domains (alpha 3, transmembrane, and ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90235-2
更新日期:1986-04-15 00:00:00
abstract::Permanent graft acceptance was induced with cyclosporine A in a low responder rat strain combination PVG (RT1(c)) to DA (RT1(av1)) of cardiac transplants. Challenge (alloimmunization) with donor and third-party WF (RT1(u)) cells was performed and followed by the analysis of alloreactive cytotoxicity. The cytotoxic T l...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1353
更新日期:1998-10-10 00:00:00
abstract::In view of the critical role played by bacillus Calmette-Guérin (BCG) in the development and functional activation of protective T cells against tuberculosis, it has become important to understand the mechanisms by which cytokines regulate BCG-mediated immune responses. There is evidence that cytokine-mediated suppres...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1662
更新日期:2000-06-15 00:00:00
abstract::The ontogeny of T cells which express major histocompatibility complex (MHC)-unrestricted T cell antigen-binding molecules (TABM) on the cell membrane was investigated. We used a rabbit anti-mouse TABM antiserum to investigate the expression of TABM by subsets of adult thymocytes, peripheral T cells, and thymocytes du...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90171-m
更新日期:1990-10-01 00:00:00
abstract::AK-5 is a spontaneously regressing rat histiocytoma and the tumor regression is mediated by CD8+ NK cells. We have investigated the functional status of effector cells mediating complete remission of the tumor. The effector-target interaction takes place with the help of antibody to AK-5 and the mechanism of lysis inv...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1056
更新日期:1994-03-01 00:00:00
abstract::An IgG1 monoclonal antibody, termed ACM-1, has been shown to react with rabbit T cells, but not Ig+ cells or macrophages. This antibody appears to recognize the same epitope as the previously described 9AE10 antibody and, together with 9AE10, has been used to obtain highly pure and fully functional T- and B-cell popul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90244-2
更新日期:1984-05-01 00:00:00
abstract::This study was undertaken to determine whether bone marrow (BM) cells contain a cell population with the capacity to induce an unresponsiveness of T cells specific to the BM self-H-2 class I antigens in vivo, i.e., veto cell population. Recombinant or congenic mice were infused intravenously with H-2-incompatible BM c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90024-l
更新日期:1990-06-01 00:00:00
abstract::We compared the effects of calorie restriction (CR) and cyclophosphamide (CTX) on the progression of lupus nephritis and immunological changes in NZB/NZW F1 mice. Ad libitum (AL)/CTX and CR delayed onset of proteinuria and significantly decreased serum levels of anti-dsDNA, anti-histone, and circulating immune complex...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2004.04.001
更新日期:2004-03-01 00:00:00
abstract::Human leucocytes from peripheral blood and tonsil were examined for the presence of the IL-3 receptor using monoclonal antibodies directed to epitopes of the alpha and beta chains of the receptor. We found that the beta chain, common to IL-3, IL-5, and GM-CSF, was either present at low levels or not detected on the ma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0049
更新日期:1996-02-25 00:00:00
abstract::We previously reported that T cell proliferation in response to a primary signal through the T cell receptor (TCR) and a costimulatory signal via the CD28 molecule is impaired in healthy, aged mice. Here we extend these studies to examine factors which may be involved in this defect in T cells from aged mice. To deter...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0144
更新日期:1996-05-25 00:00:00
abstract::Severe aplastic anemia (SAA) is a syndrome of severe bone marrow failure with high mortality. Our previous studies have demonstrated that both immature and activated DC1 increased in the bone marrow of SAA patients, and the balance of DC1 subsets shifted the stable form to active one, which might promote Th0 cells to ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.09.007
更新日期:2013-09-01 00:00:00
abstract::Cyclosporin A (CsA) was tested for its modulatory effects on the mIgM-mediated signaling of G0*-associated increases in class II MHC expression, G1-related RNA synthesis, and S phase-related DNA synthesis in human B cells. While CsA at concentrations as low as 10-100 ng/ml could completely ablate anti-IgM-induced DNA ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90213-9
更新日期:1992-04-01 00:00:00
abstract::Development of contact hypersensitivity in mice depends on the migration of Langerhans cells from the epidermis to regional lymph nodes. Since ICAM-1 and LFA-1 play important roles in leukocyte migration, we sought to determine whether in vivo administration of anti-ICAM-1 and anti-LFA-1 antibodies would inhibit conta...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1285
更新日期:1994-10-15 00:00:00
abstract::Modification of cytokine production by gender hormones has been postulated to affect disease susceptibility and outcome. Here we investigate the effect of gender and the menstrual cycle on production of cytokines. Mononuclear cells were isolated every week for 10 consecutive weeks from healthy pre-menopausal women and...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.04.013
更新日期:2008-02-01 00:00:00
abstract::A radioautographic immunolabeling technique has been developed to detect pre-B cells bearing cytoplasmic mu chains among populations of bone marrow lymphoid cells identified by conventional hematologic stains. 125I-Anti-mu antibody was applied either to fixed marrow smears, labeling total mu chains both in the cytopla...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90007-8
更新日期:1984-09-01 00:00:00
abstract::Inhibition of DPP IV has been shown to abrogate the stress-related increase in murine abortions and a concomitant increase in gamma-interferon. The aim of the present study was to investigate a potential impact of the DPP IV inhibitor Isoleucine Cyanopyrrolidide on the expression of surface antigens involved in T-cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00028-5
更新日期:2002-12-01 00:00:00
abstract::We systematically investigated the impact of the relative maturation levels of dendritic cells (DCs) on their cell surface phenotype, expression of cytokines and chemokines/chemokine receptors (by DNA array and RNase protection analyses), biological activities, and abilities to induce tumor immunity. Mature DCs expres...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1883
更新日期:2001-11-25 00:00:00
abstract::The nonspecific B-cell response induced by infecting mice with two nonlethal malaria parasites, Plasmodium chabaudi adami and Plasmodium yoelii, was analyzed in an isotype-specific reverse plaque assay. Our results showed different isotypic patterns in the two infections, although cells secreting immunoglobulin of all...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90187-x
更新日期:1985-01-01 00:00:00
abstract::pp125FAK is a novel protein tyrosine kinase associated with focal adhesions. It has been shown that ligation of VLA beta 1 integrins on a number of cell types enhanced tyrosine phosphorylation and kinase activity of pp125FAK. Cellular transformation by retroviral oncogene products or mitogenic neuropeptides also resul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1002
更新日期:1995-03-01 00:00:00
abstract::A monoclonal anti-actin antibody, 2C9, was used to study the distribution of an actin-like cell-surface antigen (hereafter termed actin) on a lymphoblastoid cell line LA350 and on human peripheral blood lymphocytes. It was determined that 8-40% of LA350 cells and 3-15% of peripheral blood lymphocytes from healthy dono...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90296-0
更新日期:1986-04-01 00:00:00
abstract::The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90302-4
更新日期:1988-04-01 00:00:00
abstract::We compared TLR responsiveness in PBMC from HIV-1-infected and uninfected individuals using the TLR agonists: TLR7 (3M-001), TLR8 (3M-002), and TLR7/8 (3M-011). Activation and maturation of plasmacytoid dendritic cells (pDC) were measured by evaluating CD86, CD40, and CD83 expression and myeloid dendritic cell (mDC) a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.01.007
更新日期:2007-11-01 00:00:00
abstract::Positive selection of T lymphocytes expressing self. MHC-restricted T cell receptors is mediated by MHC molecules expressed on thymic stroma. Among the more controversial aspects of this process is the relative role of MHC molecules on epithelial versus bone marrow-derived stromal elements (BM-APC). For CD4+ T cells, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0175
更新日期:1996-07-10 00:00:00
abstract::Our previous studies show that growth hormone overexpression (GHo) upregulates the expression of the IGF-1R and IGF-2R resulting in the protection of the EL4 lymphoma cell line from apoptosis. In this study, we report that GHo also increases TGF-beta1 protein expression measured by luciferase promoter assay, Western a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.06.003
更新日期:2006-03-01 00:00:00
abstract::We have previously demonstrated that natural killer (NK) cells are lysosome-rich and stain more intensely with lysosomotropic agents such as neutral red and quinacrine (Qu) than do non-NK cells. In this study we combined the quantitation of Qu staining with surface marker staining to define subpopulations of NK cells....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90158-x
更新日期:1988-08-01 00:00:00