Abstract：
:We previously reported that T cell proliferation in response to a primary signal through the T cell receptor (TCR) and a costimulatory signal via the CD28 molecule is impaired in healthy, aged mice. Here we extend these studies to examine factors which may be involved in this defect in T cells from aged mice. To determine if age-related changes in cytokine production might be responsible, splenic T cells from young (2-4 months) and aged (20-26 months) mice were stimulated with immobilized anti-CD3 epsilon and soluble anti-CD28 mAbs in the presence of exogenous IL-2, IL-4, IFN-gamma, IL-1 alpha, or IL-6. No improvement in the proliferative response of T cells from aged mice was found following the addition of any cytokine. In addition, the decreased proliferative response of T cells from aged mice was not caused by the enhanced production of IFN-gamma or other inhibitory factors. Interestingly, despite the age-related reduction in proliferation, no significant difference was found in the percentage of live cells entering the S, G2, or AM phase of the cell cycle in stimulated T cells from young and aged mice. Instead, anti-CD28-mediated costimulation was found to rescue T cells from young mice from anti-CD3epsilon-induced cell death, but did not rescue T cells from aged mice. This failure of T cells from aged mice to respond to costimulatory signals appears to contribute to the decreased proliferation observed from cultures containing these cells, and may be involved in many other age-related alterations in immunological responsiveness.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Engwerda CR,Handwerger BS,Fox BSdoi
10.1006/cimm.1996.0144subject
Has Abstractpub_date
1996-05-25 00:00:00pages
141-8issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(96)90144-6journal_volume
170pub_type
杂志文章abstract：:Using fetal thymic organ culture (FTOC), we describe the effects of IL-1 on T cell differentiation, particularly within the CD4-CD8- subset. While treatment of FTOC with IL-1 led to a modest reduction in total thymocyte yield, it induced an increase in the percentage of CD4-CD8- cells that express IL-2R early in cultu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90240-p
更新日期：1992-10-01 00:00:00
abstract：:In vitro incubations of CD4+ T cells with anti-CD4 monoclonal antibody (Mab) demonstrated saturation binding and inhibited both PPD-stimulated proliferation and delayed-type hypersensitivity (DTH) after passive transfer of these cells into naive rats. In comparison, in vivo administration of anti-CD4 Mab into rats, 2 ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1104
更新日期：1995-06-01 00:00:00
abstract：:Previously, we reported that human B lymphocytes can be stimulated by trinitrophenylated Brucella abortus (TNP-Ba) in vitro to generate T-independent (TI) hapten specific plaque-forming cells (PFC). Furthermore we showed that addition of Con A on Day 3 of culture enhanced the anti-TNP response. In this report the char...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90245-4
更新日期：1984-05-01 00:00:00
abstract：:We previously reported the amino acid sequence of the human macrophage chemotactic factor with a molecular weight of 1000 (MCF-1) was WLGREDGSE. In this study we examined the roles of MCF-1 and tumor necrosis factor (TNF) in a guinea pig delayed-type hypersensitivity (DTH) reaction. When macrophage chemotactic activit...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1038
更新日期：1995-04-01 00:00:00
abstract：:The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.03.023
更新日期：2011-01-01 00:00:00
abstract：:The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate th...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.09.003
更新日期：2010-01-01 00:00:00
abstract：:Double immunofluorescence studies for terminal deoxynucleotidyl transferase (TdT) and leucocyte surface membrane antigens have been used to characterize the small subpopulation of TdT-positive cells in human peripheral blood. The predominant antigens demonstrated were those coded for by the major histocompatibility co...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90223-0
更新日期：1985-02-01 00:00:00
abstract：:Macrophages are key cell types of innate immunity and play a central role in inflammation and host defense. Leukocyte-associated Ig-like receptor-1 (LAIR-1) is highly expressed on macrophages and regulates macrophage functions in several conditions. However, whether LAIR-1 is involved in governing macrophage polarizat...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.05.011
更新日期：2018-09-01 00:00:00
abstract：:Interleukin-4 (IL-4) acts directly on purified human peripheral blood B cells cultured in the presence of Epstein-Barr virus (EBV) to induce IgE secretion and to enhance the secretion of IgG and IgM. Interferon-gamma (IFN-gamma) inhibits IgE secretion in this system, without affecting the secretion of the other Ig iso...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90114-q
更新日期：1991-04-01 00:00:00
abstract：:Peripheral blood mononuclear cells (PBMCs) from cattle vaccinated with Bacillus Calmette-Guerin (BCG) were obtained and expanded in vitro by incubation with purified protein derivative. The ability of these cells to modulate the replication of virulent Mycobacterium bovis in autologous-infected macrophages was compare...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2005.01.003
更新日期：2004-11-01 00:00:00
abstract：:Blockade of the immune cell checkpoint inhibitors programmed death 1 (PD-1) and programmed death-ligand 1 (PD-L1) has become a powerful tool in cancer treatment, which is effective across various solid cancer types and hematologic malignancies. Our previous studies showed that by reducing immune tolerance, clustered r...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2017.09.003
更新日期：2017-10-01 00:00:00
abstract：:CD40 and its ligand (CD40L) regulate several cellular functions, including T and B-cell activation. The soluble form of CD40 (sCD40) antagonizes CD40/CD40L interaction. Patients undergoing hemodialysis (HD) present elevated sCD40 serum levels, which underlying molecular mechanisms are unknown. We studied sCD40 serum a...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2012.06.007
更新日期：2012-07-01 00:00:00
abstract：:The adoptive transfer of tumor-reactive T lymphocytes has recently been demonstrated to be an effective means for mediating the regression of experimental intracranial fibrosarcomas. In this study, mice bearing syngeneic intracranial GL261 gliomas were cured by the combination of sublethal whole body irradiation follo...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1140
更新日期：1997-06-15 00:00:00
abstract：:Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and greater than 95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90212-8
更新日期：1992-04-01 00:00:00
abstract：:Interleukin-1 is a potent stimulator of arachidonic acid (AA) metabolism and this activity could be attributed to the activation of the prostaglandin-forming enzyme cyclooxygenase or of the arachidonic-releasing enzyme phospholipase A2 or both. Prostaglandin E2 (PGE2), a cyclooxygenase product, and LTB4 (5-(S),12-(R)-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90323-h
更新日期：1992-11-01 00:00:00
abstract：:Experimental autoimmune encephalomyelitis was induced in a Lewis rat by sensitization with synthetic peptide GP68-86, representing the 68-86 sequence of guinea pig myelin basic protein (GPMBP). To delineate T cell determinants of GP68-86, lymph node cells from this rat were activated in culture with GP68-86 and were f...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90099-3
更新日期：1989-09-01 00:00:00
abstract：:Mice carrying a rearranged TCR Vbeta 8.2 transgene express the Vbeta protein on the vast majority of peripheral T-cells. The bone marrow and peripheral blood, as well as other lymphoid organs of both untreated animals and animals depleted of T-cells by neonatal thymectomy and/or injection from birth of monoclonal anti...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1168
更新日期：1997-10-10 00:00:00
abstract：:Activated natural killer T (NKT) cells produce a broad range of cytokines, including IL-4 and IFN-gamma, that determine immunomodulatory functions in various animal models. In this report, we show that a well-known proinflammatory cytokine, IL-17 is also produced by a distinct population of NKT cells upon TCR stimulat...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.03.004
更新日期：2008-01-01 00:00:00
abstract：:Ferric ammonium citrate (FAC) is nonmitogenic for human peripheral blood mononuclear cells (PBM) but has a potent mitogenic activity in the presence of IL-2. FAC in the presence of IL-2 increases the number of human peripheral blood mononuclear cells (PBM) expressing receptors for IL-2 and transferrin. FAC also marked...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90105-k
更新日期：1991-04-01 00:00:00
abstract：:Many studies have already been reported with regard to the serological cross-reactivities between the polymorphic determinants of murine Ia antigens and human HLA-DR antigens. In this paper, we examined the biological cross-reactivity of the polymorphism of Class II antigens in the xenogeneic antigen-presenting cell (...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90033-9
更新日期：1984-09-01 00:00:00
abstract：:ErbB-2 is ubiquitinated and degraded when dissociated from its membrane chaperone or bound by specific antibody. Reagents which induce such degradation have demonstrated antitumor activity and may impact ErbB-2 immunogenicity. To further understand ErbB-2 degradation and immunogenicity, a glycine-alanine repeat (GAr) ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2001.1853
更新日期：2001-09-15 00:00:00
abstract：:Thirty-two cord blood samples taken after caesarean section or vaginal delivery and concurrent venous blood samples obtained from normal adult controls were evaluated using monoclonal antibodies. The percentage of circulating pan-T-cell+ lymphocytes was significantly lower in cord blood (46%) compared with adult contr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90069-3
更新日期：1985-04-15 00:00:00
abstract：:There are still questions about whether macrophage differentiation is predetermined or is induced in response to tissue microenvironments. C2D macrophage cells reside early in the macrophage lineage in vitro, but differentiate to a more mature phenotype after adoptive transfer to the peritoneal cavity (PEC-C2D). Since...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.06.012
更新日期：2011-01-01 00:00:00
abstract：:Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus transformed B cells is mainly mediated by LFA-1 (CD11a/CD18) and CD2 molecules. Low-affinity binding of resting T cells can be transiently upregulated by cross-linking of CD3-TCR (T cell receptor) complexes. This inside-out signaling influences i...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1211
更新日期：1993-09-01 00:00:00
abstract：:A low culture temperature of 27 degrees C inhibited mouse primary in vitro anti-hapten plaque-forming cell responses to a thymus-dependent (TD) antigen (Ag) (trinitrophenyl-keyhole limpet hemocyanin, TNP-KLH). In contrast, the magnitudes of secondary responses to TNP-KLH or primary responses to a thymus-independent (T...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90327-n
更新日期：1990-04-01 00:00:00
abstract：:CCR5 is a chemokine receptor expressed by T cells and macrophages, which also functions as the principal coreceptor for macrophage (M)-tropic HIV-1 strains to enter the host cells. In this study, we aim to better understand the ligand-binding profiles of CCR5 and the chemokine-receptor usage on leukocyte cells. We fou...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1379
更新日期：1998-11-01 00:00:00
abstract：:In this study, cellular requirements for rejection are examined by the use of adoptive transfer assays in the ACI to Lewis cardiac allograft model. The findings show that adoptive transfer of 1 x 10(8) spleen cells (SpL), 5 x 10(7) T-cells, and 2 x 10(7) helper T-cells (W3/25+) obtained from normal, nonsensitized dono...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90109-3
更新日期：1989-11-01 00:00:00
abstract：:Thymic (T) or bursal (B) lymphocytes from chicks sensitized to Mycobacterium tuberculosis produce an avian lymphocyte inhibitory factor (LyIF). The physiochemical properties of both T and B LyIF were established by ultrafiltration which yielded four fractions with molecular weight ranges of greater than 100,000; 50,00...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90080-1
更新日期：1983-07-15 00:00:00
abstract：:Murine peritoneal macrophages (M phi), activated in vivo or in vitro, remarkably inhibited the uptake of thymidine by a lens epithelial cell line, while resident M phi, or M phi induced by thioglycollate, exhibited much lower or no cytostatic capacity. The target cells were partially protected from the cytostatic acti...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90222-3
更新日期：1984-01-01 00:00:00
abstract：:Sensitized T lymphocytes can mediate potent antitumor effects when transferred to tumor-bearing animals. Employing the MCA 105 and MCA 106 sarcomas, we were able to generate antitumor effector cells by immunization of syngeneic mice with tumor cells admixed with Corynebacterium parvum. These immune splenocytes could b...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90209-8
更新日期：1989-05-01 00:00:00
