Abstract：
:Mice carrying a rearranged TCR Vbeta 8.2 transgene express the Vbeta protein on the vast majority of peripheral T-cells. The bone marrow and peripheral blood, as well as other lymphoid organs of both untreated animals and animals depleted of T-cells by neonatal thymectomy and/or injection from birth of monoclonal anti-TCR antibodies, contain a small population of cells that express low levels of the Vbeta transgene product, but no T-cell or other detectable lineage-specific phenotypic markers. When such TG-bearing BM cells are purified and injected directly into the non-TG thymus, they show the phenotypic maturation sequences of intrathymic T-cell development and, subsequently, mature TG-bearing peripheral T-cells. However, this population failed to support long-term recovery from lethal irradiation. Both Vbeta 8.2 TG and CD3delta mRNA transcripts are strongly expressed in the cell population, but no CD3gamma, CD3epsilon, CD3zeta, CD4, CD8beta, pre-Talpha, or RAG-1 transcript was detected. The transgene-encoded TCR component is not bound to the cell membrane exclusively by a phosphatidylinositol linkage. The data show that the fully rearranged TCR transgene and transcripts for at least one of the associated CD3 components, CD3delta, can be expressed on a subpopulation of BM and PBL cells that has not passed through the thymus. The phenotypic characteristics of this cell population resemble those described for the earliest thymocyte described by others. The TG protein molecule in this model may provide a specific developmental marker for a prothymocyte lineage subset that lacks pluripotential properties.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Lincoln GC,Sheng B,Odebralski J,Lucchetta R,Smith RTdoi
10.1006/cimm.1997.1168subject
Has Abstractpub_date
1997-10-10 00:00:00pages
1-12issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(97)91168-0journal_volume
181pub_type
杂志文章abstract：:The oral administration of myelin basic protein (MBP) to Lewis rats prior to an encephalitogenic challenge resulted in total inhibition or a significant delay in the onset of experimental autoimmune encephalomyelitis (EAE). In vitro lymphocyte proliferative responses to MBP were significantly decreased in MBP-fed rats...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90305-x
更新日期：1988-04-01 00:00:00
abstract：:In vitro incubations of CD4+ T cells with anti-CD4 monoclonal antibody (Mab) demonstrated saturation binding and inhibited both PPD-stimulated proliferation and delayed-type hypersensitivity (DTH) after passive transfer of these cells into naive rats. In comparison, in vivo administration of anti-CD4 Mab into rats, 2 ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1104
更新日期：1995-06-01 00:00:00
abstract：:Activation of lck-fyn kinases during T cell receptor signaling leads to Vav phosphorylation, activation of downstream targets including Rac1, and a transient decline in ezrin and moesin phosphorylation. We have shown that age increases Rac1 activity and lowers ezrin and moesin phosphorylation in resting mouse CD4 cell...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.06.001
更新日期：2009-01-01 00:00:00
abstract：:This study was conducted on human K562 lymphocytes to investigate the mechanisms implicated in the regulation of the serotonin transport process. The uptake of serotonin in these cells was saturable (Km, 3.37 microM; Vmax, 2.03 nmol/10(6) cells) and Na+ dependent; isoosmotic replacement of Na+ with choline chloride in...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0242
更新日期：1996-09-15 00:00:00
abstract：:Mesenchymal stromal cells (MSCs) are promising candidates for developing cell therapies for intractable diseases. To assess the feasibility of transplantation with human umbilical cord blood (hUCB)-derived MSCs, we analyzed the ability of these cells to function as alloantigen-presenting cells (APC) in vitro. hUCB-MSC...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.04.003
更新日期：2008-02-01 00:00:00
abstract：:As an atypical guanine nucleotide exchange factor (GEF), Dock5 has been extensively studied in cellular functions. However, the role of Dock5 on B-cell immunity still remain elusive. In this study, we generated a Dock5 knockout mouse model to study the effect of Dock5 deficiency on B cell development, differentiation ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2019.01.001
更新日期：2019-03-01 00:00:00
abstract：:Guinea pig myelin basic protein (MBP) was inserted into phosphatidylserine liposomes and Lewis rats were injected by the intracardiac (ic) route with 75 microgram doses of MBP-liposomes according to various schedules. After challenge with 75 microgram guinea pig MBP in complete Freund's adjuvant, the rats were followe...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90088-1
更新日期：1984-03-01 00:00:00
abstract：:Antigen-specific signal transduction leading to IL2 induction and secretion in the T cell line 171 is augmented by association of p56lck with CD4. Although no change in cytoplasmic calcium level ([Ca2+]i) was detectable during antigen-specific signal transduction of 171-CD4+ cells, IL2 induction was inhibited by FK506...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1255
更新日期：1994-10-01 00:00:00
abstract：:The aim of the study was to investigate the interaction between manLAM and DC-SIGN influencing DCs maturation and downstream immune response using small interfering RNA-expressing lentiviral vectors to specifically knockdown DC-SIGN. Our data indicated that DC-SIGN knockdown alone in DCs did not affect the maturation ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.09.001
更新日期：2011-01-01 00:00:00
abstract：:Some further aspects of B-lymphocyte-promotor factor (B-LPF) activity have been studied. This activity was present in the supernatants of certain helper-T-cell lines, and it induced polyclonal activation of Ig+ B cells into Ig-secreting cells. It was found that B-LPF induced polyclonal, terminal B-cell differentiation...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90067-x
更新日期：1985-04-15 00:00:00
abstract：:Twenty-seven different CD45 monoclonal antibodies (mAb) were assessed for their ability to block cytotoxicity of alloreactive CD4+ MHC class II-specific or CD8+ class I-specific human T cell clones (n = 3 and 5, respectively). Twelve of 27 blocked the former but only 1/27 the latter, although all 27 significantly inhi...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90151-g
更新日期：1990-05-01 00:00:00
abstract：:Our strategy to use saturation mutagenesis to produce an unbiased collection of major histocompatibility class I mutants has resulted in unpredicted mutant phenotypes. First, we have shown data supporting our earlier work of the Dp20(Y27N) mutant. Allorecognition is altered at the clonal level while no variation in ly...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90020-r
更新日期：1990-06-01 00:00:00
abstract：:Although it is well established that the processes of cellular proliferation and apoptosis are linked, the role of cell cycle regulators in T cell responses in vivo is not well understood. In recent years, tumor suppressor molecule p19(ARF) has emerged as a key cell cycle regulator important in cellular apoptosis agai...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/s0008-8749(02)00597-x
更新日期：2002-10-01 00:00:00
abstract：:Chronic relapsing experimental allergic encephalomyelitis (CR-EAE) can be adoptively transferred using myelin basic protein (BP)-specific helper T cell lines, and suppressor cells may be important in recovery from EAE. In order to generate suppressor cells, spleen cells obtained from BP-complete Freund's adjuvant (CFA...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90322-i
更新日期：1990-04-01 00:00:00
abstract：:The clinical outcome of Leishmania pathogenesis ranges from active skin lesions to fatal visceral dissemination and severely impaired T cell immunity. It is well established that a strong Th1 immune response is protective against cutaneous forms of the disease, however a mixed Th1/Th2 response is most commonly observe...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2016.07.004
更新日期：2016-11-01 00:00:00
abstract：:Estrogen displays potent immunoregulatory properties. In the present study we investigated the in vivo effect of estrogen on natural killer (NK) cell cytotoxic activity in several mouse strains. After castration, 17 beta-estradiol was administered in low or high doses. After 3-6 weeks of hormone administration an in v...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1262
更新日期：1994-10-01 00:00:00
abstract：:Our earlier studies revealed that a rapid and progressive loss of splenic NK activity in mice during the development of a number of transplanted tumors as well as of spontaneous tumors was due to an inactivation of natural killer (NK) lineage cells rather than to their disappearance. The mechanism of this inactivation...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90133-9
更新日期：1985-07-01 00:00:00
abstract：:Senescence is characterized by several alterations in the immune system. Such modifications can be found in lymphoid organs as well as in the cellular components of the immune system. Several reports have suggested that immune dysfunction can affect both T and B cells, but T cells have been shown to be more susceptibl...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.01.010
更新日期：2009-01-01 00:00:00
abstract：:The membrane phenotype of T cells involved in delayed hypersensitivity (DH), protective immunity, and suppression of delayed hypersensitivity to herpes simplex virus (HSV) has been determined. T cells from immune lymph nodes transferring DH and antiviral immunity to normal recipients were characterized as Lyt 1+2-. Th...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90332-5
更新日期：1983-02-01 00:00:00
abstract：:The cellular requirements for stimulating primed alloreactive T cells have been investigated. In vitro-primed secondary alloreactive cells, long-term lines, and Ly 1+2- noncytolytic clones which reacted with allo-H-2K, D, or Mls (M locus) antigens were tested. The data indicated that a specialized antigen-presenting c...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90032-2
更新日期：1985-03-01 00:00:00
abstract：:Although most intraepithelial lymphocytes (IEL) in mouse small intestine bear surface markers classically associated with T lymphocytes, the T-cell nature of these cells remains controversial. In the present study IEL from normal mice, or from mice infected with the gut nematode Trichinella spiralis, were therefore te...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90093-6
更新日期：1986-12-01 00:00:00
abstract：:Semaphorins are important regulators of peripheral T and B-cell mediated immune responses in mice and humans. Modulatory roles of semaphorins in T cell development are also being characterized. We carefully analyzed the gene expression and protein levels of semaphorins 4A, 4D, and 7A at various developmental stages of...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.03.006
更新日期：2013-02-01 00:00:00
abstract：:To investigate the process of tolerance induction we have developed an in vivo model using TCR beta-chain transgenic mice tolerized with the superantigen staphylococcal enterotoxin B. We have previously demonstrated that tolerized peripheral T cells were anergic when stimulated in vitro with immunogenic peptides, supe...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1177
更新日期：1994-07-01 00:00:00
abstract：:To understand the role of cAMP during macrophage activation, we investigated the effects of various cAMP analogues in the induction of nitric oxide (NO) synthesis in murine peritoneal macrophages. Intracellular cAMP modulators such as N6,O2'-dibutyryl cyclic AMP (DB-cAMP), 8-bromo-cAMP, or 8-chloro-cAMP had no effect ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1144
更新日期：1997-07-10 00:00:00
abstract：:We tested the effects of salen manganese (Salen-Mn) complexes, which are scavengers of reactive oxygen species exhibiting superoxide dismutase and catalase activities on the rejection of and alloresponse to fully allogeneic skin grafts in mice. We showed that pre-transplant treatment of C57Bl/6 donor skin or of BALB/c...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.06.009
更新日期：2006-06-01 00:00:00
abstract：:Intercellular adhesion molecule (ICAM)-1, or CD54, is a member of the immunoglobulin superfamily that binds to lymphocyte function-associated antigen-1 and macrophage-1 antigen. ICAM-1:LFA-1/Mac-1 interaction may be involved in both activation and extravasation of leukocytes. To determine the roles of ICAM-1 in the de...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1395
更新日期：1998-11-25 00:00:00
abstract：:Dendritic cell (DC)-based immunotherapy has promising for treatment of non-small cell lung cancer (NSCLC). Melanoma-associated antigen 3 (MAGE-A3) is a tumor-specific antigen and expressed in approximately 35-40% of NSCLC tissues. Calreticulin (CALR) is a protein chaperone and can enhance DC maturation and antigen pre...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.12.003
更新日期：2016-02-01 00:00:00
abstract：:The biological effects of IL-2 are mediated through high (complex of alpha and beta chain) or intermediate (beta chain) affinity IL-2 receptors. Previously, chimeric proteins composed of IL-2 and Pseudomonas exotoxin (IL-2-PE) were shown to be specifically cytotoxic to cells bearing IL-2 receptors. It has also been sh...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90029-o
更新日期：1992-09-01 00:00:00
abstract：:Macrophages are highly plastic immune cells with temporally distinct transcriptome changes upon lipopolysaccride (LPS) activation. However, to what extent transcriptome reprogramming is mediated via spatial chromatin looping is not well studied. We generated high resolution chromatin interaction maps for LPS-stimulate...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2020.104148
更新日期：2020-09-01 00:00:00
abstract：:It is becoming increasingly clear that regulatory T cells (Treg cells) in specific tissues are important parts of immune system. Tissue-resident Treg cells, which are largely Foxp3-expressing CD4(+) Treg cells, are distinct from one another and conventional Treg cells, and have tissue-specific phenotype and function. ...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2015.06.005
更新日期：2015-09-01 00:00:00
