Monoclonal anti-actin antibody recognizes a surface molecule on normal and transformed human B lymphocytes: expression varies with phase of cell cycle.

Abstract:

:A monoclonal anti-actin antibody, 2C9, was used to study the distribution of an actin-like cell-surface antigen (hereafter termed actin) on a lymphoblastoid cell line LA350 and on human peripheral blood lymphocytes. It was determined that 8-40% of LA350 cells and 3-15% of peripheral blood lymphocytes from healthy donors stain specifically with 2C9, almost exclusively on IgM-positive cells. Treatment of cells with 2C9 prior to incubation caused cell-surface actin to first patch and then to cap. Treatment of cells with nonspecific protease caused a loss of surface actin, with reexpression of the marker after 8-12 hr. The expression of LA350 surface actin also increased with DNA synthesis and was demonstrated to be maximal during late G1/early S phase. Thus, this antigen may be a sensitive marker for activated lymphocytes. These studies contribute to our understanding of the expression and distribution of actin-like membrane proteins that may participate in regulatory signals mediated by anti-actin antibody.

journal_name

Cell Immunol

journal_title

Cellular immunology

authors

Bach MA,Lewis DE,McClure JE,Parikh N,Rosenblatt HM,Shearer WT

doi

10.1016/0008-8749(86)90296-0

subject

Has Abstract

pub_date

1986-04-01 00:00:00

pages

364-74

issue

2

eissn

0008-8749

issn

1090-2163

pii

0008-8749(86)90296-0

journal_volume

98

pub_type

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