Abstract:
:AK-5 is a spontaneously regressing rat histiocytoma and the tumor regression is mediated by CD8+ NK cells. We have investigated the functional status of effector cells mediating complete remission of the tumor. The effector-target interaction takes place with the help of antibody to AK-5 and the mechanism of lysis involves ADCC. The immune lymphocytes displayed higher ADCC against AK-5 and spontaneous NK activity against YAC1 targets when compared with normal lymphocytes. Our observations demonstrate a direct correlation between antitumor antibody response and the activation of NK cell function in tumor-rejecting animals. The cold target inhibition assay indicated that both the ADCC and spontaneous NK activities were expressed by an identical or overlapping clonotype. The tumor-infiltrating lymphocytes were effective in killing the tumor by ADCC, while the freshly isolated TILs from actively regressing tumors exhibited reduced in vitro cytotoxicity. Exposure of immune splenocytes to AK-5 or YAC1 cells in vitro resulted in the concomitant loss of ADCC as well as spontaneous NK activity, indicating that both the activities are expressed by common lytic pathway and the inactivation of TILs may be due to target-directed exocytosis of NK lytic factors. Based on these results, we propose that the spontaneous regression of AK-5 tumor is achieved by direct lysis of antibody-tagged AK-5 cells by tumor-infiltrating NK cells and the intrinsic ability of the activation/reactivation of NK cells may restore their effector function to participate in the complete regression of the tumor.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Bright JJ,Hegde SP,Begum Z,Khar Adoi
10.1006/cimm.1994.1056subject
Has Abstractpub_date
1994-03-01 00:00:00pages
54-65issue
1eissn
0008-8749issn
1090-2163pii
S0008874984710562journal_volume
154pub_type
杂志文章abstract::Antigen-specific T-helper factor (ThF) of CBA (H-2k) origin in the picryl (TNP) contact sensitivity system (Mr 60-70 kDa) was reduced with dithiothreitol under mild conditions. Affinity chromatography on antigen yielded an antigen-binding chain (Mr 20-30 kDa) and an antigen-nonbinding chain (Mr 40-50 kDa). Both chains...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90037-8
更新日期:1988-05-01 00:00:00
abstract::Viruses are related to the etiology of periodontitis. However, the role of viruses on Th17 cells infiltration in periodontitis lesions is unknown. Therefore, we examined the effects of TLR3 ligand on CCL20, which is related to Th17 cells migration, production in human gingival fibroblasts (HGFs). Polyinosinic-polycyti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.05.005
更新日期:2013-05-01 00:00:00
abstract::Tumors harbor a population of cancer stem cells (CSCs) which can drive tumor progression and therapeutical resistance. Nature killer (NK) cells are best known for their ability to directly recognize and kill malignant cells. However, the susceptibility of cancer stem cells to NK cells is not fully understood. Here we ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.11.009
更新日期:2016-02-01 00:00:00
abstract::Staphylococcal enterotoxin B (SEB) is a potent polyclonal activator of both human and murine T cells. We previously reported data which show that SEB-induced T cells suppress antibody secretion by various mouse plasmacytoma cell lines. This suppression of antibody secretion was found to be both idiotype and isotype no...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90227-i
更新日期:1990-09-01 00:00:00
abstract::We previously reported the amino acid sequence of the human macrophage chemotactic factor with a molecular weight of 1000 (MCF-1) was WLGREDGSE. In this study we examined the roles of MCF-1 and tumor necrosis factor (TNF) in a guinea pig delayed-type hypersensitivity (DTH) reaction. When macrophage chemotactic activit...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1038
更新日期:1995-04-01 00:00:00
abstract::PNK-E and G7 mAbs regulate porcine NK and ADCC activities by binding to distinct NK function-associated trigger molecules on porcine NK cells. Previous work demonstrates that PNK-E mAb binds to a 205-kDa tetrameric molecule composed of two 47-kDa peptides and two 50-kDa peptides and G7 mAb binds to a distinct 40-kDa h...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1026
更新日期:1993-02-01 00:00:00
abstract::Infection of certain strains of mice, such as C57BL/6 and C57BL/10 [B10], with LP-BM5 murine leukemia virus (MuLV) rapidly causes a profound and lethal immune suppression. The H2d congenic strain of B10, B10.D2, is resistant to disease, but B10 x B10.D2 F1 mice are susceptible, indicating that disease sensitivity is d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0086
更新日期:1996-04-10 00:00:00
abstract::Despite significant improvements in the overall survival of patients with multiple myeloma (MM) over the past 15 years, the disease remains incurable. Treatment options are limited for patients who have relapsed or are refractory to immunomodulatory drugs (IMiDs), proteasome inhibitors, and monoclonal antibodies. In t...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2018.10.003
更新日期:2018-12-01 00:00:00
abstract::Macrophages are key cell types of innate immunity and play a central role in inflammation and host defense. Leukocyte-associated Ig-like receptor-1 (LAIR-1) is highly expressed on macrophages and regulates macrophage functions in several conditions. However, whether LAIR-1 is involved in governing macrophage polarizat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.05.011
更新日期:2018-09-01 00:00:00
abstract::As fascinating a molecule as it can potentially get, nuclear factor-κB (NF-κB), a regulatory transcription factor, is as intriguing. NF-κB is a dimeric complex that controls the transcription of essential genes. NF-κB is involved in a variety of responses that play a pivotal role in regulating the immune response to i...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2011.06.021
更新日期:2011-01-01 00:00:00
abstract::To improve the engraftment of human peripheral blood lymphocytes (PBL) to severe combined immunodeficient (SCID) mice, and to elucidate the factors which prevent the PBL's survival, we treated SCID mice with mAb, which neutralizes murine IFN-gamma's ability to activate cell-mediated immunity. Compared with untreated m...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1238
更新日期:1998-01-10 00:00:00
abstract::Neuroinflammation plays a pivotal role in the incidence and progression of Alzheimer's disease (AD). Cathelicidin-related antimicrobial peptide (CRAMP) is critically involved in the innate neuronal responses of chronic neuroinflammation in AD and thus plays a key role in the disease. Here, we show that Aβ42 induced mi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.05.008
更新日期:2018-09-01 00:00:00
abstract::It has been reported that a muramyl dipeptide (MDP) treatment regimen (200 micrograms MDP per mouse, Days -4, -3, -2, and -1) that, given prophylactically, affords protection against several infectious agents also induces lymph node hyperplasia, lymph node cell (LNC) hyperresponsiveness to mitogens, and spleen cell hy...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90248-5
更新日期:1985-04-01 00:00:00
abstract::Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca(2+)-dependent activation pathway. In the present experiments we studied the pr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.01.004
更新日期:2013-01-01 00:00:00
abstract::The proliferative responses of human peripheral blood mononuclear cells (PBL) and thymocytes to OKT3 monoclonal antibody have been investigated. The PBL response to OKT3 was maximal after 72 hr while that of thymocytes was inappreciable at all times measured. Unlike phytohemagglutinin, OKT3 was unable to elicit the mi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90105-9
更新日期:1984-04-01 00:00:00
abstract::Epstein-Barr virus (EBV) readily immortalizes human peripheral blood lymphocytes (PBL) in vitro. However, during the past several years, we found that PBL from two exceptional EBV-seropositive healthy adult individuals were refractory to immortalization by EBV. We report here a study aimed at learning about the immuno...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90090-7
更新日期:1989-09-01 00:00:00
abstract::Neutrophils exhibit intrinsic sinusoidal metabolite concentration oscillations of 3 min in resting cells and an additional approximately 10- or 20-s oscillation in migrating/adhering cells. To better understand immune complex (IC)-mediated leukocyte activation, we have studied neutrophil metabolic oscillations in the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1481
更新日期:1999-05-25 00:00:00
abstract::Spontaneous rosette formation in humans is restricted to a subpopulation of the circulating T cells. We have previously shown that the interaction between lymphocytes and autologous red blood cells (auto-RBC) is not mediated by a self-recognition mechanism, since allogeneic (allo-) RBC bind to T cells through the same...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90345-4
更新日期:1985-11-01 00:00:00
abstract::Dendritic cells (DC), as professional antigen presenting cells, play the central role in the process of body initiating the anti-tumor immunity, and the study on DC anti-tumor vaccine has become heated in recent years. In this study, we used polyethylene glycol (PEG) to induce renal cell carcinoma (RCC) 786-O cell lin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.01.004
更新日期:2010-01-01 00:00:00
abstract::In the mouse, activation of T cells by T cell receptor (TCR) crosslinking with anti-CD3 antibodies in the absence of a costimulatory signal induces Th1 but not Th2 cell anergy. Furthermore, anti-CD3 induces anergy of Th1- but not Th2-type lymphokine secretion in Th0 cells. This study was designed to determine whether ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1199
更新日期:1995-10-01 00:00:00
abstract::B-cell clonal expansions (BCE) in young mice are transient, detectable for less than 4 weeks. In contrast, BCE in old mice persist more than 2 months. The greater persistence of BCE in old mice does not appear to be due to the age of the host as the survival of phenyloxazolone chicken serum albumin-induced BCE in most...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.01.002
更新日期:2004-09-01 00:00:00
abstract::A series of anti-DNA antibody-producing hybridomas were obtained by fusing spleen cells from 6-month-old MRL/lpr, autoimmune prone, mice with P3X63-Ag8 myeloma cells. Rabbit anti-idiotype (id) antibodies specific for several of the hybridoma proteins were prepared. It was shown that the anti-id antibody inhibited immu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90056-6
更新日期:1987-03-01 00:00:00
abstract::The membrane phenotype of T cells involved in delayed hypersensitivity (DH), protective immunity, and suppression of delayed hypersensitivity to herpes simplex virus (HSV) has been determined. T cells from immune lymph nodes transferring DH and antiviral immunity to normal recipients were characterized as Lyt 1+2-. Th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90332-5
更新日期:1983-02-01 00:00:00
abstract::Class II major histocompatibility antigens (Ia) play a major role in regulating T-B cell interactions; therefore, regulation of the amount of Ia on B cells may be an important point of control in the immune response. Mitogens in human and murine systems have been reported to increase Ia expression on B cells, and in t...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90108-6
更新日期:1988-02-01 00:00:00
abstract::To determine whether human antibody responses to T cell-independent pneumococcal polysaccharide antigens are derived from CD5+ or CD5- B cells, we utilized an ELISPOT assay to detect individual anti-polysaccharide antibody-secreting cells. Human anti-type IV pneumococcal polysaccharide antibody-secreting cells were fo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90006-b
更新日期:1992-08-01 00:00:00
abstract::Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus transformed B cells is mainly mediated by LFA-1 (CD11a/CD18) and CD2 molecules. Low-affinity binding of resting T cells can be transiently upregulated by cross-linking of CD3-TCR (T cell receptor) complexes. This inside-out signaling influences i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1211
更新日期:1993-09-01 00:00:00
abstract::Antigen processing in B cells is initiated by antigen binding to the surface B cell antigen receptor (BCR). The BCR is a signaling receptor which also functions to endocytose bound antigen for subsequent intracellular processing and presentation with class II molecules. Previously, using subcellular fractionation, we ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1264
更新日期:1998-02-25 00:00:00
abstract::To characterize the binding properties of the Fc receptors on human macrophages, the binding of radiolabeled human IgG1 to peritoneal macrophages was assessed. Cells were obtained at the time of diagnostic laparoscopy from women undergoing evaluation of infertility. Macrophages bound on the average more IgG1 monomer t...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90403-9
更新日期:1984-07-01 00:00:00
abstract::Guinea pig myelin basic protein (MBP) was inserted into phosphatidylserine liposomes and Lewis rats were injected by the intracardiac (ic) route with 75 microgram doses of MBP-liposomes according to various schedules. After challenge with 75 microgram guinea pig MBP in complete Freund's adjuvant, the rats were followe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90088-1
更新日期:1984-03-01 00:00:00
abstract::MHC class II-restricted antigen presentation requires trafficking of newly synthesized class II-invariant chain complexes from the trans-Golgi network to endosomal, peptide-loading compartments. This transport is mediated by dileucine-like motifs within the cytosolic tail of the invariant chain. Although these signals...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1817
更新日期:2001-06-15 00:00:00