Abstract：
:PNK-E and G7 mAbs regulate porcine NK and ADCC activities by binding to distinct NK function-associated trigger molecules on porcine NK cells. Previous work demonstrates that PNK-E mAb binds to a 205-kDa tetrameric molecule composed of two 47-kDa peptides and two 50-kDa peptides and G7 mAb binds to a distinct 40-kDa heterodispersed monomeric peptide on porcine NK cells. The data presented herein demonstrate that all PNK-E+ PBLs are G7+ and all G7+ PBLs are PNK-E+ indicating that the PNK-E and G7 molecules are coexpressed by porcine NK cells. Bound G7 mAb blocks subsequent binding of PNK-E mAb but not the converse. Bound F(ab')2 G7 mAb abrogates the ability of whole PNK-E mAb to enhance NK activity but bound F(ab')2 PNK-E mAb has no affect on G7 mAb enhancement of NK activity. PNK-E mAb enhanced NK activity is inhibited by binding of F(ab')2 G7 mAb even though whole PNK-E mAb remains bound. However, bound F(ab')2 PNK-E mAb has no affect on G7 mAb-enhanced NK activity. When PNK-E and G7 mAbs were tested alone and together in NK assays, comparable levels of enhancement were observed. PNK-E and G7 hybridomas express surface mAb through which NK cells bind and specifically lyse these hybridomas. Lysis of PNK-E and G7 hybridomas is inhibited by pretreatment of PBLs with F(ab')2 G7 mAb. These data indicate a physical association between the PNK-E and G7 molecules on NK cells and suggest that the G7 molecule is external to the PNK-E molecule.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Wierda WG,Johnson BD,Dato ME,Kim YBdoi
10.1006/cimm.1993.1026subject
Has Abstractpub_date
1993-02-01 00:00:00pages
270-83issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(83)71026-9journal_volume
146pub_type
杂志文章abstract：:A series of anti-DNA antibody-producing hybridomas were obtained by fusing spleen cells from 6-month-old MRL/lpr, autoimmune prone, mice with P3X63-Ag8 myeloma cells. Rabbit anti-idiotype (id) antibodies specific for several of the hybridoma proteins were prepared. It was shown that the anti-id antibody inhibited immu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90056-6
更新日期：1987-03-01 00:00:00
abstract：:Seeking common abnormalities in mice genetically predisposed to lupus-like autoimmune disease, we investigated (1) the ontogeny of Ia antigens (I-A/I-E) on the surfaces of resident peritoneal macrophages (rpM phi) of lupus and normal mice, (2) spontaneous and lectin-induced in vitro production of M phi-stimulating fac...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90133-3
更新日期：1984-08-01 00:00:00
abstract：:We have examined noninduced cytotoxicity of mouse gut associated and peripheral lymphoid tissues for a wide variety of syngeneic as well as allogeneic cell lines and lymphoblasts. Lymphoid cells from Peyer's patches were found to lyse these targets in a 3-hr chromium release assay whereas lymphoid cells from intestina...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90158-9
更新日期：1986-09-01 00:00:00
abstract：:Upregulation of immunoglobulin D-specific receptors (IgD-R) on CD4+ T cells may facilitate their interaction with specific carbohydrate moieties uniquely associated with membrane IgD on B cells. Previous studies have shown that upregulation of IgD-R facilitates cognate T-B cell interactions by mediating bidirectional ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2000.1747
更新日期：2001-02-01 00:00:00
abstract：:The mechanism by which purinergic agonists modulate murine T-lymphocyte activation and proliferation was investigated. Adenosine and other compounds such as ATP and 2-chloroadenosine (ClAdo) were found to block T-cell mitogenesis induced by concanavalin A (Con A) in a dose-dependent fashion. The nonmetabolizable adeno...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90199-1
更新日期：1986-08-01 00:00:00
abstract：:During the gestational cycle the placental tissue does not express class II MHC antigens and whether this phenomenon is important to fetal survival has not yet been evoked. It has been reported that class II antigen expression precedes renal and cardiac graft rejection, which may also be the case in fetal abortion. In...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90039-t
更新日期：1990-07-01 00:00:00
abstract：:The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we com...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90083-9
更新日期：1987-04-01 00:00:00
abstract：:CD8+ T cell differentiation is controlled by the transcription factors T-bet and Eomesodermin, in concert with the cytokines IL-2, IL-10 and IL-12. Among these pathways, the mechanisms by which T-box proteins and IL-10 interact to promote a memory T cell fate remain poorly understood. Here, we show that Eomes and IL-1...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.11.008
更新日期：2019-01-01 00:00:00
abstract：:The many signals that control the progress of various immune responses to both foreign and self antigens can be divided into no less than three major groups. The first group is the initial positive stimulus, associated with activation events through antigen receptors and their associated proteins. These signals launch...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/0008-8749(90)90174-p
更新日期：1990-10-01 00:00:00
abstract：:Although most intraepithelial lymphocytes (IEL) in mouse small intestine bear surface markers classically associated with T lymphocytes, the T-cell nature of these cells remains controversial. In the present study IEL from normal mice, or from mice infected with the gut nematode Trichinella spiralis, were therefore te...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90093-6
更新日期：1986-12-01 00:00:00
abstract：:It has been reported that the three-dimensional structure of thymic epithelial cells (TECs) is responsible for thymic positive selection but that this ability disappears when TECs are cultured in monolayer. These results have supported the hypothesis that certain TEC-specific molecules are extinguished during monolaye...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2001.1823
更新日期：2001-07-10 00:00:00
abstract：:The majority of autoimmune diseases have a strong gender bias, affecting mostly females. Gender-specific factors like sex-hormones, the presence or absence of a second X chromosome, and gender-specific gut microbiota may contribute to this bias. In this review we will discuss the role of the X chromosome encoded toll-...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2014.12.002
更新日期：2015-04-01 00:00:00
abstract：:The kinetics of viability of lymph node and spleen cells of mice genetically selected for "high" or "low" in vitro lymphocyte responsiveness to PHA were studied in PHA or PPD-stimulated short-term cultures. Lo/PHA cells were found to be less viable than Hi/PHA cells in unstimulated control cultures. PHA improved the v...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90008-4
更新日期：1983-04-01 00:00:00
abstract：:This report compares the ability of cyclosporin A and FK-506 to inhibit human T cell activation triggered via cell surface molecules that utilize different intracellular processes. We stimulated highly purified peripheral blood T lymphocytes with mitogens (Con A and PHA), ionomycin + PMA, or monoclonal antibodies spec...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90103-i
更新日期：1991-04-01 00:00:00
abstract：:The development of B cell tolerance is believed to involve negative signaling to B cells derived from the binding of antigen to the B cell surface immunoglobulin. B cell lines that receive negative signals may provide useful models for studying the mechanisms of B cell tolerance. We have established a human B lymphoma...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1314
更新日期：1994-12-01 00:00:00
abstract：:Mygalin is an antibacterial molecule isolated from the hemocytes of the spider Acanthoscurria gomesiana. It was identified as bis-acylpolyamine spermidine. We evaluated the modulator effects of synthetic Mygalin in the innate immune response. We demonstrate that Mygalin induces IFN-γ synthesis by splenocytes increasin...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2012.04.003
更新日期：2012-01-01 00:00:00
abstract：:Normal spleen cells cultured in high or low concentrations of interleukin (IL) 2 for 3 days contain Thy-1+ CD4- CD8+ cells that powerfully suppress primary but not ongoing or active lymphocyte responses. The precursors of these cells are Thy-1+ AGM-1- and are absent or present in greatly diminished numbers in athymic ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90111-1
更新日期：1989-11-01 00:00:00
abstract：:When lymphoid cells from rats recovered from experimental autoimmune encephalomyelitis (EAE) were incubated in vitro for 1 hr with myelin basic protein (BP), then washed and transferred along with anti-BP immune serum to naive recipients, those recipients immediately developed a solid, long-lasting resistance to activ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90272-4
更新日期：1989-10-01 00:00:00
abstract：:Mice that received a sublethal, intraperitoneal dose of viable Listeria monocytogenes, virulent strain 10403, exhibited a systemic increase in natural killer (NK) activity. The kinetics of the response differed with respect to the various effector cell populations analyzed. Resident peritoneal cells and peripheral blo...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90108-4
更新日期：1984-04-01 00:00:00
abstract：:Dendritic cells initiate the immune response by presenting antigen in the context of varying levels of costimulation. The maturation state of the dendritic cell determines the quantity and quality (Th1, Th2) of the subsequent T cell response. Members of the NF-kappaB family of transcription factors have previously bee...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/s0008-8749(03)00114-x
更新日期：2003-04-01 00:00:00
abstract：:Blood and tissue eosinophilia is obtained when mice pretreated with cyclophosphamide (CY) and sensitized with picryl chloride are challenged on each ear lobe on day 13. To gain important insights into the cellular mechanisms involved in CY-induced eosinophilia in the contact-sensitivity reaction, we examined the cytok...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2000.1677
更新日期：2000-08-01 00:00:00
abstract：:Serum of an autoimmune MRL/Mp-lpr/lpr (MRL/l) mouse supported the proliferation of interleukin 3 (IL-3)-dependent cell line, FDC-P2. This IL-3-like activity initially appeared at 1 month of age and increased with age. Females showed higher titers than did males. MRL/Mp-+/+ mouse sera also exhibited such activity, thou...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90030-0
更新日期：1989-07-01 00:00:00
abstract：:Our previous studies show that growth hormone overexpression (GHo) upregulates the expression of the IGF-1R and IGF-2R resulting in the protection of the EL4 lymphoma cell line from apoptosis. In this study, we report that GHo also increases TGF-beta1 protein expression measured by luciferase promoter assay, Western a...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.06.003
更新日期：2006-03-01 00:00:00
abstract：:Inherited deficiency of the enzyme adenosine deaminase (ADA) has been found in a significant proportion of patients with severe combined immunodeficiency disease and inherited defect generally characterized by a deficiency of both B and T cells. Two questions are central to understanding the pathophysiology of this di...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90317-x
更新日期：1985-10-15 00:00:00
abstract：:Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is caused by mutations in the AIRE gene. Murine studies suggest that AIRE controls thymic expression of tissue-restricted antigens, its absence allowing nonselected autoreactive cells to escape. We tested this in humans using the TCRβ CDR3 length ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.03.005
更新日期：2015-06-01 00:00:00
abstract：:The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90302-4
更新日期：1988-04-01 00:00:00
abstract：:AK-5 is a spontaneously regressing rat histiocytoma and the tumor regression is mediated by CD8+ NK cells. We have investigated the functional status of effector cells mediating complete remission of the tumor. The effector-target interaction takes place with the help of antibody to AK-5 and the mechanism of lysis inv...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1056
更新日期：1994-03-01 00:00:00
abstract：:We showed previously that the expressions of various src family protein tyrosine kinases (PTKs) were induced independently during the monocytic differentiation of HL-60 cells. The role of PTKs was further assessed in the present study by investigating the effects of PTK inhibitors on the differentiation. It was demons...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90196-v
更新日期：1992-04-01 00:00:00
abstract：:We examined the effects of a rare sugar, D-allose, which is 6-carbon monosaccharide, on endocytosis and T cell stimulation by dendritic cells (DCs). The endocytosis of BCG-anti-BCG immune complexes by DCs markedly decreased in D-allose-containing medium. Co-culture with T cells (mixed leukocyte reaction, MLR) of DCs, ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.06.015
更新日期：2011-01-01 00:00:00
abstract：:Inhibition of DPP IV has been shown to abrogate the stress-related increase in murine abortions and a concomitant increase in gamma-interferon. The aim of the present study was to investigate a potential impact of the DPP IV inhibitor Isoleucine Cyanopyrrolidide on the expression of surface antigens involved in T-cell...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/s0008-8749(03)00028-5
更新日期：2002-12-01 00:00:00
