Abstract:
:This report compares the ability of cyclosporin A and FK-506 to inhibit human T cell activation triggered via cell surface molecules that utilize different intracellular processes. We stimulated highly purified peripheral blood T lymphocytes with mitogens (Con A and PHA), ionomycin + PMA, or monoclonal antibodies specific for cell surface antigens involved in activation (CD2, CD3, CD28) either in combination with each other or in conjunction with PMA. Using measurements of the proliferative response, IL-2 production, and changes in intracellular Ca2+ ([Ca2+]i), we demonstrate that FK-506 exerts its inhibitory effect on early events of T-cell activation in a manner indistinguishable from that of CsA. An important finding in this study is the strict correlation between those activation pathways that are inhibited by FK-506 and CsA and the requirement that the sensitive pathways induce a measurable rise in [Ca2+]i. This correlation held even for the CD28/CD2 pathway which was previously shown to be calcium-independent; however by employing FACS analysis of [Ca2+]i within individual cells, a subset of cells activated via CD28/CD2 was found to respond with a measurable rise in [Ca2+]i. We also noted that the proliferative response induced by certain stimuli, such as ionomycin + PMA and PHA + PMA, was partially resistant to FK-506 and CsA, while IL-2 production was completely suppressed. The partial FK-506/CsA-resistance of these responses was shown to be determined by the amount of PMA added to the cultures. We conclude from our investigations that FK-506 and CsA inhibit highly similar signal transduction pathways in human T lymphocytes.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Lin CS,Boltz RC,Siekierka JJ,Sigal NHdoi
10.1016/0008-8749(91)90103-isubject
Has Abstractpub_date
1991-04-01 00:00:00pages
269-84issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(91)90103-Ijournal_volume
133pub_type
杂志文章abstract::Using analogues of arginine to inhibit nitric oxide (NO.) production, investigators have demonstrated the intermediary role of NO. in a variety of physiological events including the antimicrobial activity exhibited by macrophages in vitro. In an effort to establish the effector function of NO. in the antimicrobial act...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1048
更新日期:1994-02-01 00:00:00
abstract::Putative monocytic myeloid-derived suppressor cells (mMDSC; lineage-HLA-DR-/lo) were generated in 7-day cultures from normal rhesus macaque bone marrow (BM) cells in GM-CSF and IL-6. Three subsets were identified based on their differential expression of CD14, CD33, CD34 and CD11b. Following flow sorting, assessment o...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.04.013
更新日期:2018-07-01 00:00:00
abstract::Neuroinflammation plays a pivotal role in the incidence and progression of Alzheimer's disease (AD). Cathelicidin-related antimicrobial peptide (CRAMP) is critically involved in the innate neuronal responses of chronic neuroinflammation in AD and thus plays a key role in the disease. Here, we show that Aβ42 induced mi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.05.008
更新日期:2018-09-01 00:00:00
abstract::As an atypical guanine nucleotide exchange factor (GEF), Dock5 has been extensively studied in cellular functions. However, the role of Dock5 on B-cell immunity still remain elusive. In this study, we generated a Dock5 knockout mouse model to study the effect of Dock5 deficiency on B cell development, differentiation ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.01.001
更新日期:2019-03-01 00:00:00
abstract::IL-5 is a pleiotropic cytokine that promotes eosinophil differentiation and survival. While naïve bronchial epithelial cells (BEC) produce low levels of IL-5, the role of BEC-derived IL-5 in allergic airway inflammation is unknown. We now show that BEC, isolated from mice with OVA-induced allergic airway disease (AAD)...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.04.008
更新日期:2010-01-01 00:00:00
abstract::Murine C57BL/6 thymocytes were cultivated together with syngeneic thymus-derived macrophages (TDM phi) for up to 96 hr to determine whether TDM phi participate in thymocyte maturation. The expression level of H-2b and Thy-1.2 antigens served as thymocyte differentiation surface markers as analyzed by flow cytometry. I...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90310-8
更新日期:1991-05-01 00:00:00
abstract::Here, we established CD4(+)αβTh1 clones specific for rat vascular smooth muscle antigen (VSMAg) that induced vasculitis lesions in the lungs of MRL/Mp-Fas(+/+) mice following adoptive transfer. Six different T cell clones, MV1b1 (Vβ1), MV1b4 (Vβ4), MV1b8.3 (Vβ8.3), MV1b61 (Vβ6), MV1b62 (Vβ6), and MV1b63 (Vβ6), were is...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.03.004
更新日期:2016-05-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis (MS), and is induced by immunization with disease-causative self-antigens such as myelin oligodendrocyte glycoprotein (MOG). We have previously reported that transplantation of MOG expressing thymic epithelial progenitors (TEPs) ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.10.007
更新日期:2017-12-01 00:00:00
abstract::Interleukin-1 is a potent stimulator of arachidonic acid (AA) metabolism and this activity could be attributed to the activation of the prostaglandin-forming enzyme cyclooxygenase or of the arachidonic-releasing enzyme phospholipase A2 or both. Prostaglandin E2 (PGE2), a cyclooxygenase product, and LTB4 (5-(S),12-(R)-...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90323-h
更新日期:1992-11-01 00:00:00
abstract::Accumulating evidence indicates that inflammatory signals required for maximizing effector T cell generation have opposing effects on the development of memory T cell precursors. Toll-like receptor (TLR)2, and TLR9 significantly contribute to the inflammatory milieu and therefore in this study we examined whether the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.12.009
更新日期:2016-04-01 00:00:00
abstract::Prevention of ultraviolet radiation- or chemical carcinogen-induced morphologic transformation and inhibition of tumor-producing transformed cell growth by lymphotoxin and by normal spleen leukocytes were quantitatively compared to define the antineoplastic activity spectra of these natural immune mediators. When Syri...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90372-6
更新日期:1983-03-01 00:00:00
abstract::Mice infected with murine acquired immunodeficiency syndrome (MAIDS) virus developed lymphoadenopathy and profound immunodeficiency. Concomitantly the expression of endogenous mammary tumor virus (MTV) mRNA increased significantly, especially for the 1.7-kb 3' open reading frame (ORF) mRNA encoding MTV superantigen. B...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1326
更新日期:1998-08-01 00:00:00
abstract::We have compared the interaction of Mycobacterium tuberculosis with the human macrophage-like cell line THP-1 and with human monocyte-derived macrophages (MDM). The association of M. tuberculosis with THP-1 and MDM was comparable in both the presence and the absence of serum. For both cells, serum-mediated binding was...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1554
更新日期:1999-10-10 00:00:00
abstract::A previous report (G. E. Woloschak and D. Senitzer, submitted for publication) has demonstrated that mitogen-stimulated splenocytes fuse at a much higher frequency than untreated splenocytes as measured by the fusion index (a calculation of the number of nuclei in fused cells vs the total number of nuclei). A measurem...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90059-x
更新日期:1983-07-01 00:00:00
abstract::A majority (42/62) of TCR2+ interleukin 2-dependent human T lymphocyte clones was found concordantly to express not only the CD28 co-receptor structure at the cell surface but also its ligand B7. Interactions between CD28 and B7 can have important consequences for T cell activation, particularly in providing "signal 2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1238
更新日期:1993-10-15 00:00:00
abstract::Interleukin (IL)-4 has been shown to be secreted simultaneously with IL-2 and interferon (IFN)-gamma by the majority of CD4+ human T cell clones isolated and cultured using IL-2 as a growth factor. Moreover, IL-4 was found to be as efficient as IL-2 to promote the outgrowth of human T cell clones. In this study we hav...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90283-h
更新日期:1991-07-01 00:00:00
abstract::Conditioned medium (CM) from 24-hr culture of guinea pig L2C B lymphoblastic leukemia cells contained an inhibitor(s) of mitogen- and antigen-stimulated proliferation of syngeneic (strain 2 guinea pigs), allogeneic (Hartley guinea pigs), and xenogeneic (Balb/c mouse, NZW rabbit) lymphocytes. The proliferation of sever...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90087-6
更新日期:1987-04-01 00:00:00
abstract::The transfer of B cells from mice immunized with Type III pneumococcal polysaccharide (SSS-III) results in the activation of suppressor and amplifier T cells that control the magnitude of the antibody response in recipient mice, immunized subsequently with SSS-III. Prior treatment of transferred B cells with an excess...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90368-5
更新日期:1985-12-01 00:00:00
abstract::We previously reported that T cell proliferation in response to a primary signal through the T cell receptor (TCR) and a costimulatory signal via the CD28 molecule is impaired in healthy, aged mice. Here we extend these studies to examine factors which may be involved in this defect in T cells from aged mice. To deter...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0144
更新日期:1996-05-25 00:00:00
abstract::Interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) are major proinflammatory cytokines inducing the synthesis and release of many inflammatory mediators. They are involved in immune regulation, autoimmune diseases, and inflammation. Acanthoic acid, (-)-pimara-9(11),15-dien-19-oic acid, is a pimaradiene d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0154
更新日期:1996-06-15 00:00:00
abstract::We have previously used human Epstein Barr virus (EBV)-transformed B lymphoblastoid cell lines for the identification and purification of a novel cytokine, natural killer cell stimulatory factor (NKSF/IL-12), that has pleiotropic effects on human lymphocytes. B cell lines are also routinely employed as feeder cells fo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90322-g
更新日期:1992-11-01 00:00:00
abstract::Monolayers of freshly isolated human monocytes are known to ingest particulate activators of the human alternative complement pathway. The ingestion of rabbit erythrocytes, ER, by human monocytes in serum-free medium was studied. The process is Mg2+-dependent and optimum phagocytic activity was obtained at approximate...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90024-x
更新日期:1988-05-01 00:00:00
abstract::Peripheral blood lymphocytes (PBL) isolated from BALB/c mice bearing a B-cell leukemia (BCL1) showed a marked proliferative response upon two days culturing with poly(L-lysine) (PLL) of various molecular weights. An inverse relationship was noted between the molecular weight of the PLL and the dose required for optima...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90203-5
更新日期:1985-02-01 00:00:00
abstract::OVA-specific T cells were immortalized by infection with radiation leukemia virus (RadLV). Some clones derived from such population were shown to exhibit helper activity. We then tested clones without such function and found among them some that secreted macrophage migration inhibition factor (MIF) and leukocyte migra...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90328-x
更新日期:1986-10-01 00:00:00
abstract::In vitro ultraviolet-B (UVB) irradiation of murine and rodent bone marrow cells prevents GVHD without compromising engraftment while inducing tolerance to donor-type allografts. In anticipation of clinical trials of UVB-modified bone marrow grafts, we studied the in vitro effects of UVB irradiation (50-300 J/m2) on hu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1202
更新日期:1995-10-15 00:00:00
abstract::Macrophages are highly plastic immune cells with temporally distinct transcriptome changes upon lipopolysaccride (LPS) activation. However, to what extent transcriptome reprogramming is mediated via spatial chromatin looping is not well studied. We generated high resolution chromatin interaction maps for LPS-stimulate...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104148
更新日期:2020-09-01 00:00:00
abstract::The mechanism underlying the apparent differences in the capacity of murine and human class I MHC molecules to function as signal transducing structures in T cells was examined. Cross-linking murine class I MHC molecules on splenic T cells did not stimulate an increase in intracellular calcium ([Ca2+]i) and failed to ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90254-m
更新日期:1992-10-15 00:00:00
abstract::Macrophages are key cell types of innate immunity and play a central role in inflammation and host defense. Leukocyte-associated Ig-like receptor-1 (LAIR-1) is highly expressed on macrophages and regulates macrophage functions in several conditions. However, whether LAIR-1 is involved in governing macrophage polarizat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.05.011
更新日期:2018-09-01 00:00:00
abstract::Spontaneously cytotoxic cells possessing specificity and having a complex pattern of reactivity directed at least partly against self-determinants develop by the age of 10 weeks in the autoimmune mouse strain MRL/Mp-lpr/lpr (MRL-lpr) and by the age of 6 months in C57BL/6-lprl/lpr. Similar effector cells do not develop...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90003-2
更新日期:1988-04-15 00:00:00
abstract::G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T lymphocytes. Given that mature T cells which lack antigen receptors (CDl-Ti) are refractory to stimulation through CD2 or other accessory molecules, T cell rece...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90050-2
更新日期:1990-07-01 00:00:00