Abstract:
:It has been reported that the three-dimensional structure of thymic epithelial cells (TECs) is responsible for thymic positive selection but that this ability disappears when TECs are cultured in monolayer. These results have supported the hypothesis that certain TEC-specific molecules are extinguished during monolayer culture. In this study, using MHC class II-restricted T-cell receptor transgenic mice, we demonstrated that preselected CD4(+)8(+) (DP) thymocytes were inhibited from developing into CD4(+)8(-) (CD4SP) cells in reaggregate thymus organ culture with monolayer-cultured TECs, but this inhibition was removed when TECs were cultured in monolayer with protein synthesis inhibitor or when the cultured TECs were treated with fixative. These results seem to be inconsistent with the previous hypothesis and indicate that monolayer culture allows TECs to retain the surface molecules necessary for positive selection but interferes with their function, which must be sustained for three dimensional structure.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Sato T,Sato C,Suzuki D,Yoshida Y,Nunomura S,Matsumura T,Hozumi K,Habu Sdoi
10.1006/cimm.2001.1823subject
Has Abstractpub_date
2001-07-10 00:00:00pages
71-9issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(01)91823-4journal_volume
211pub_type
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