Apoptotic cell death induced by anti-IgM antibody and phorbol esters is inhibited by interleukin-4 in human B lymphoma cell line MBC-1.


:The development of B cell tolerance is believed to involve negative signaling to B cells derived from the binding of antigen to the B cell surface immunoglobulin. B cell lines that receive negative signals may provide useful models for studying the mechanisms of B cell tolerance. We have established a human B lymphoma cell line, MBC-1, positive for both surface IgM and IgD. The growth of MBC-1 cells is inhibited by anti-IgM antibody but not by anti-IgD antibody. The rapid time course of MBC-1 cell death, the morphologic feature of dying cells, and DNA fragmentation indicate that surface IgM cross-linking induces apoptotic cell death. Interestingly, interleukin-4 (IL-4) could rescue MBC-1 cells from this apoptotic signal. BCL-2 protein is neither expressed nor induced in MBC-1 cells. The treatment of MBC-1 cells with IL-4 does not interfere with mobilization of Ca2+ or induce any phenotypical change. In addition, phorbol 12-myristate 13-acetate and phorbol 12, 13-dibutyrate also induced growth inhibition followed by apoptotic cell death in MBC-1 cells. IL-4 is able to protect MBC-1 cells from cell death, but not from growth inhibition induced by protein kinase C activators. The results collectively suggest that IL-4 could inhibit the transduction of apoptotic signal following the activation of protein kinase C in MBC-1 cells.


Cell Immunol


Cellular immunology


Komada Y,Zhang XL,Zhou YW,Tanaka S,Higashigawa M,Ido M,Sakurai M




Has Abstract


1994-12-01 00:00:00














  • Pancreatic cancer derived exosomes regulate the expression of TLR4 in dendritic cells via miR-203.

    abstract::MicroRNAs (miRNAs) are aberrant in many human tumors which can be transferred to immune cells by tumor-derived exosomes. Dendritic cells (DCs) play an important role in activation of immune response. However, the effect of tumor-derived exosomes on toll-like receptor (TLR) in DCs remains unclear. We investigated the i...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Zhou M,Chen J,Zhou L,Chen W,Ding G,Cao L

    更新日期:2014-11-01 00:00:00

  • The priority dispute over the function of the lymphatic system and Glisson's ghost (the 18th-century Hunter-Monro Feud).

    abstract::Basic Immunology has had only two significant public priority disputes. The first began in the late 1650s and concerned the recognition of the peripheral network of vessels which collects lymph throughout the body. The publication of this major anatomical discovery prompted a priority feud discussed in a previous pape...

    journal_title:Cellular immunology

    pub_type: 历史文章,杂志文章


    authors: Ambrose CT

    更新日期:2007-01-01 00:00:00

  • Omega-3 polyunsaturated fatty acid impairment of early host resistance against Listeria monocytogenes infection is independent of neutrophil infiltration and function.

    abstract::The primary objective of this study was to determine whether the n-3 PUFA-mediated changes in host response to a Listeria monocytogenes infection (e.g., cytokine production and bacterial clearance) were dependent upon neutrophils. Balb/c mice were fed one of two semi-purified diets that contained either 0 or 41 g of n...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Fritsche K,Irons R,Pompos L,Janes J,Zheng Z,Brown C

    更新日期:2005-05-01 00:00:00

  • In vitro production of antigen-specific T cells from unprimed mice: role of dexamethasone and anti-IL-10 antibodies.

    abstract::We describe here a culture system for studying the development, in vitro, of antigen-specific CD4 T cells from unprimed mice. T cells from young mice are initially exposed to antigen, such as pigeon cytochrome C or keyhole limpet hemocyanin, in the presence of adherent accessory cells and then allowed to proliferate i...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Dozmorov I,Miller RA

    更新日期:1997-06-15 00:00:00

  • Dock5 controls the peripheral B cell differentiation via regulating BCR signaling and actin reorganization.

    abstract::As an atypical guanine nucleotide exchange factor (GEF), Dock5 has been extensively studied in cellular functions. However, the role of Dock5 on B-cell immunity still remain elusive. In this study, we generated a Dock5 knockout mouse model to study the effect of Dock5 deficiency on B cell development, differentiation ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Chen A,Yang D,Xuan X,Miller H,Luo X,Yu J,Yang G,Wang H,Liu C

    更新日期:2019-03-01 00:00:00

  • Generation of phosphatidic acid and diacylglycerols following ligation of surface immunoglobulin in human B lymphocytes: potential role in PKC activation.

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Valentine MA,Bursten SL,Harris WE,Draves KE,Pollok BA,Ostrowski J,Bomsztyk K,Clark EA

    更新日期:1992-05-01 00:00:00

  • CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes.

    abstract::Non-obese diabetic (NOD) mice develop spontaneous T-cell responses against pancreatic beta-cells, leading to islet cell destruction and diabetes. Despite high genetic similarity, non-obese resistant (NOR) mice do not develop diabetes. We show here that spleen cells of both NOD and NOR mice respond to the islet cell an...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ott PA,Anderson MR,Tary-Lehmann M,Lehmann PV

    更新日期:2005-05-01 00:00:00

  • Role of C chemokine lymphotactin in mediating recruitment of antigen-specific CD62L(lo) cells in vitro and in vivo.

    abstract::In this study we investigated whether T cells expressing high or low levels of CD62L were differentially susceptible to the T cell chemokine lymphotactin. We found that lymphotactin induced preferential migration of antigen-specific (CD62L(lo)) T cells over the nonspecific (CD62L(hi)) T cells in vitro and in vivo. The...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kurt RA,Bauck M,Harma S,McCulloch K,Baher A,Urba WJ

    更新日期:2001-05-01 00:00:00

  • Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression.

    abstract::The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Bohacova P,Kossl J,Hajkova M,Hermankova B,Javorkova E,Zajicova A,Krulova M,Holan V

    更新日期:2020-01-01 00:00:00

  • Production of biologically active recombinant human soluble CD23 and its effect on PBMCs isolated from hyper-IgE blood.

    abstract::A recombinant form of human soluble CD23 (sCD23), the low affinity receptor for IgE (FcepsilonRII), was produced by PCR cloning the lectin-binding domain sequence into a bacterial expression vector. After renaturation and purification, the sCD23 bound IgE and divalent metal ions, indicating its activity. The recombina...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Daniels BB,Askew SL,van de Venter M,Oosthuizen V

    更新日期:2005-04-01 00:00:00

  • A proliferation-inducing ligand (APRIL) induced hyper-production of IgA from tonsillar mononuclear cells in patients with IgA nephropathy.

    abstract::IgA nephropathy (IgAN) is a tonsil-related disease. We previously showed that oligodeoxynucleotides with CpG (CpG-ODN) and B-cell activation factor (BAFF) are involved in hyperproduction of IgA from tonsillar mononuclear cells of patients with IgAN (IgAN-TMCs). In this study, we focused on a proliferation-inducing lig...

    journal_title:Cellular immunology

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    authors: Takahara M,Nagato T,Nozaki Y,Kumai T,Katada A,Hayashi T,Harabuchi Y

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  • Triggering HLA-DR molecules on human peripheral monocytes induces their death.

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Thibeault A,Zekki H,Mourad W,Charron D,Al-Daccak R

    更新日期:1999-02-25 00:00:00

  • L3T4+ T-cell-independent reactivity of Lyt2+ T cells in vivo.

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Heeg K,Steeg C,Wagner H

    更新日期:1988-01-01 00:00:00

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    abstract::Recently, it has been reported that IL-4 production by murine Th2 cell lines is insensitive to inhibition by E-type prostaglandins. In the present study, IL-4 production in vitro by freshly isolated concanavalin A (Con A)-stimulated murine spleen cells was readily suppressed by PGE2 with an I50 of 2 nM. Comparable sup...

    journal_title:Cellular immunology

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    authors: Parker CW,Huber MG,Godt SM

    更新日期:1995-02-01 00:00:00

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Newcomb JR,Lin YS,Rogers TJ

    更新日期:1990-09-01 00:00:00

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    journal_title:Cellular immunology

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    authors: Mongini PK,Tolani S,Fattah RJ,Inman JK

    更新日期:2002-03-01 00:00:00

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    journal_title:Cellular immunology

    pub_type: 杂志文章,评审


    authors: Banerjee A,Bhattacharya P,Joshi AB,Ismail N,Dey R,Nakhasi HL

    更新日期:2016-11-01 00:00:00

  • Transduction of human melanoma cells with interleukin-2 gene reduces tumorigenicity and enhances host antitumor immunity: a nude mouse model.

    abstract::Human melanoma tumor cells were genetically modified in vitro by transferring the interleukin-2 (IL-2) gene via a retroviral vector into established or fresh tumor cells. In addition, human melanoma cells were transduced in vivo by the direct injection of the IL-2/retroviral vector into melanoma xenografts in nude mic...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Abdel-Wahab Z,Li WP,Osanto S,Darrow TL,Hessling J,Vervaert CE,Burrascano M,Barber J,Seigler HF

    更新日期:1994-11-01 00:00:00

  • Leukocyte complement: interleukin-like properties of factor Bb.

    abstract::It has been previously shown that the activated form of Factor B (Factor Bb) of the alternative pathway of complement activation stimulates monocyte spreading and killing of xenogenic erythrocytes and staphylococci. Factor Bb also stimulates lymphocyte blastogenesis in vitro, and native (uncleaved) Factor B is a major...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Hirani S,Fair DS,Papin RA,Sundsmo JS

    更新日期:1985-05-01 00:00:00

  • Monoclonal anti-actin antibody recognizes a surface molecule on normal and transformed human B lymphocytes: expression varies with phase of cell cycle.

    abstract::A monoclonal anti-actin antibody, 2C9, was used to study the distribution of an actin-like cell-surface antigen (hereafter termed actin) on a lymphoblastoid cell line LA350 and on human peripheral blood lymphocytes. It was determined that 8-40% of LA350 cells and 3-15% of peripheral blood lymphocytes from healthy dono...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Bach MA,Lewis DE,McClure JE,Parikh N,Rosenblatt HM,Shearer WT

    更新日期:1986-04-01 00:00:00

  • Differential effect on fresh and cultured T cells of PHA-induced changes in free cytoplasmic calcium: relation to IL-2 receptor expression, IL-2 production, and proliferation.

    abstract::PHA stimulation of both freshly isolated and IL-2-dependent cultured T cells induced a rapid rise in intracytoplasmic calcium concentration. Chelation of extracellular calcium with EGTA resulted in a failure of PHA to induce a rise in intracytoplasmic calcium, resulting in the fresh T cells in an inhibition of IL-2 pr...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Watman NP,Crespo L,Davis B,Poiesz BJ,Zamkoff KW

    更新日期:1988-01-01 00:00:00

  • A clinical correlation of anti-DNA-AGE autoantibodies in type 2 diabetes mellitus with disease duration.

    abstract::Nonenzymatic glycation of amino groups of DNA bases by reducing sugars can generate advanced glycation end products (AGEs). Cellular formation of AGEs under normal physiology is continuously scanned and removed by efficient system in the cells. However, excess formation and accumulation of AGEs may be cause or consequ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ashraf JM,Arfat MY,Arif Z,Ahmad J,Moinuddin,Alam K

    更新日期:2015-02-01 00:00:00

  • Induction of apoptosis in activated T cell blasts by suppressive macrophages: a possible immunotherapeutic approach for treatment of autoimmune disease.

    abstract::Large suppressive macrophages (LSM) were induced by restimulating spleen cells from rats with experimental autoimmune myasthenia gravis (EAMG) in vitro, with the autoantigen acetylcholine receptor (AChR) in the presence of cyclosporine A. LSM, purified from these cultures, are extremely potent suppressors of AChR-stim...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: McIntosh KR,Drachman DB

    更新日期:1999-04-10 00:00:00

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Xie J,Wang W,Si JW,Miao XY,Li JC,Wang YC,Wang ZR,Ma J,Zhao XC,Li Z,Yi H,Han H

    更新日期:2013-01-01 00:00:00

  • Lymphocyte subsets involved in tumor-activated nonspecific feedback suppression.

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ingenito GG,Calkins CE

    更新日期:1983-07-01 00:00:00

  • Ia- and IgG-Fc receptor-positive accessory cell sustains peripheral T lymphocyte but not thymocyte mitogenesis induced by OKT3 monoclonal antibody.

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Musiani P,Maggiano N,Aiello FB,Lauriola L,Ranelletti FO,Piantelli M

    更新日期:1984-04-01 00:00:00

  • Induction of hyporesponsiveness in human lymphocytes-T despite their expression of both the co-receptor CD28 and its ligand B7.

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Friccius H,Siegels-Hübenthal P,Rehbein A,Schlotz E,Pawelec G

    更新日期:1993-10-15 00:00:00

  • Regulation of antibody secretion by hybridoma cells. II. Mechanism of idiotype-induced suppression of antibody secretion by hybridoma cells.

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    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Gulati P,Falck-Pedersen E,Siskind GW

    更新日期:1989-10-15 00:00:00

  • Dialyzable leukocyte extract (transfer factor) in the treatment of superinfected fistulating tuberculosis of the bone.

    abstract::The effect of the addition of dialyzable leukocyte extract (DLE)(transfer factor) to tuberculostatic drugs in the treatment of superinfected fistulating tuberculosis of bones and joints was evaluated in a controlled study. Eleven patients whose disease had persisted for a mean of 20 +/- 4.8 years and had proved to be ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Zielinski CC,Savoini E,Ciotti M,Orani R,Königswieser H,Eibl MM

    更新日期:1984-03-01 00:00:00

  • VCAM-1 influences lymphocyte proliferation and cytokine production during mixed lymphocyte responses.

    abstract::The allogeneic mixed lymphocyte reaction (MLR) is regarded as an effective model for examining the events which occur during an allospecific immune response. Numerous studies have delineated the role of adherence molecule interactions during the MLR response. In the present study we have identified VCAM-1 as having a ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Lukacs NW,Strieter RM,Evanoff HL,Burdick MD,Kunkel SL

    更新日期:1994-03-01 00:00:00