Abstract:
:Patients treated with monoclonal antibodies and cytokines for cancer receive often co-medication, which may influence treatment efficacy. Therefore, we investigated with a flowcytometric cytotoxicity assay the effect of several immunomodulatory drugs on antibody dependent cellular cytotoxicity (ADCC), interleukin-2 (IL-2) induced cytotoxicity and IL-2-induced-ADCC. We found that dexamethasone markedly inhibited the IL-2 induced cytotoxicity and the IL-2-induced-ADCC. Ondansetron, a 5-HT-3 serotonin receptor antagonist augmented significantly ADCC. Clemastine, a histamine type-2 receptor antagonist augmented the IL-2-induced-ADCC. The TNF antagonist thalidomide suppressed ADCC whereas pentoxifylline proved to be ineffective. Other tested drugs namely ibuprofen and indomethacin, both prostaglandin E2 antagonists, cimetidine a histamine type-2 receptor antagonist, the opioid pethidine, prostaglandin E2 and histamine exerted minor effects or had no influence on the tested parameters. We conclude that glucocorticosteroids should be avoided with monoclonal antibody and cytokine treatment. According to our in vitro data the other drugs tested did not have a negative impact on cellular cytotoxicity and ADCC.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Flieger D,Varvenne M,Kleinschmidt R,Schmidt-Wolf IGdoi
10.1016/j.cellimm.2007.05.002subject
Has Abstractpub_date
2007-03-01 00:00:00pages
26-33issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(07)00083-4journal_volume
246pub_type
杂志文章abstract::The quantitative organ distribution and tissue microenvironment positioning of radioisotopically labeled cloned T cells were characterized. Intravenous (iv) injection of 51chromium (51Cr)-labeled, long-term cultured cloned T-helper cells and cells from several cloned cytolytic T-lymphocyte lines (CTLL) resulted in poo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90349-0
更新日期:1983-02-15 00:00:00
abstract::Differences in gender immune response have resulted in differences in immune protection and susceptibility to inflammatory diseases. Cultured peripheral blood mononuclear cells (PBMC) are widely used in immunomodulation studies, yet the influence of gender is usually not considered. We examined the effect of in vitro ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.10.009
更新日期:2012-01-01 00:00:00
abstract::The systemic adoptive transfer of activated T cells, derived from tumor-draining lymph nodes (LNs), mediates the regression of established tumors. In this study, the requirement of cell adhesion molecules, CD11a/CD18 (LFA-1), CD54 (ICAM-1), CD49d/CD29 (VLA-4), and CD106 (VCAM-1), for T cell infiltration into tumors an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1439
更新日期:1999-03-15 00:00:00
abstract::It is still a matter of debate, whether tolerance toward self-non-MHC antigens is due to intrathymic deletion or to regulatory processes in the periphery. To further pursue this question, responsiveness toward TNP and an anti-TNP monoclonal antibody (Sp6) carrying a recurrent idiotype was evaluated in prenatally trini...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90298-6
更新日期:1990-03-01 00:00:00
abstract::The murine T cell hybridoma line, MBI-1.15, secretes a 17-kDa protein which decreases binding activity of the CD23 molecule for its natural ligand, IgE. This protein, denoted epsilon receptor-modulating protein (epsilon RMP), was previously characterized and shown to be a novel serine protease. The present studies sho...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1036
更新日期:1994-02-01 00:00:00
abstract::In view of the eminently important role of interleukin-2 (IL-2) in T-cell responses, and in view of reports about immune stimulatory effects of PGE2, we reinvestigated the question whether PGE2 inhibits IL-2 production. It was found that PGE2 does not inhibit IL-2 production in murine spleen cell cultures after optima...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90180-7
更新日期:1985-01-01 00:00:00
abstract::Recent studies have indicated that Macrophage migration inhibitory factor (MIF) plays an important role in the prevention and treatment of asthma. However the role of MIF in airway inflammation and airway epithelial barrier disruption in house dust mite (HDM)-induced asthma has not been addressed. We hypothesized that...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.103965
更新日期:2020-01-01 00:00:00
abstract::PHA stimulation of both freshly isolated and IL-2-dependent cultured T cells induced a rapid rise in intracytoplasmic calcium concentration. Chelation of extracellular calcium with EGTA resulted in a failure of PHA to induce a rise in intracytoplasmic calcium, resulting in the fresh T cells in an inhibition of IL-2 pr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90060-3
更新日期:1988-01-01 00:00:00
abstract::The role of macrophages (M phi) and that of splenic dendritic cells (DC) in the allogeneic mixed leukocyte reaction (MLR) in the mouse have been investigated. In contrast with the high stimulatory capacity of DC, we obtained no evidence in favor of the competence of M phi, whether Ia + or Ia-, as an autonomous stimula...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90169-2
更新日期:1984-10-15 00:00:00
abstract::A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1279
更新日期:1998-05-01 00:00:00
abstract::The MCA 102 sarcoma has been defined by a variety of immunologic studies as a tumor lacking intrinsic immunogenicity. Nevertheless, we have recently demonstrated the feasibility of generating therapeutically effective lymphocytes for adoptive immunotherapy of this tumor. Procedures to achieve this required in vivo pri...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90201-2
更新日期:1990-08-01 00:00:00
abstract::Blood and tissue eosinophilia is obtained when mice pretreated with cyclophosphamide (CY) and sensitized with picryl chloride are challenged on each ear lobe on day 13. To gain important insights into the cellular mechanisms involved in CY-induced eosinophilia in the contact-sensitivity reaction, we examined the cytok...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1677
更新日期:2000-08-01 00:00:00
abstract::Transformed murine fetal thymocyte cell lines were derived by incubating fetal thymic organ cultures with a v-myc/v-raf-containing retroviral construct in order to model developmental stages within the early triple negative (CD3-CD4-CD8-) thymocyte population. The resulting 10 cell lines had a lymphoid morphology, wer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0170
更新日期:1996-07-10 00:00:00
abstract::Many studies have shown that tumor necrosis factor (TNF) is a potent soluble mediator of immunoregulation and inflammation. Neuropeptide substance P (SP) has been known to exert significant influence on production of certain inflammatory cytokine by immune cells. Immunopathogenic mechanism underlying the effect of neu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.9969
更新日期:1995-12-01 00:00:00
abstract::That M1/70, a monoclonal anti-murine macrophage antibody, recognizes murine natural killer cells (NK) and that there is an increase in NK following intraperitoneal infection with live Listeria monocytogenes (LM) was previously reported. Here, LM-induced NK cells were further characterized with respect to tumor target ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90206-5
更新日期:1984-11-01 00:00:00
abstract::We studied the effect of vasoactive intestinal peptide (VIP), somatostatin (SOM), and substance P (SP) on IL-4-stimulated human IgE and IgG subclass production. VIP and SOM, but not SP, inhibited IgE production without affecting IgM or IgA production by mononuclear cells (MNC) from nonatopic donors from 10 pM to 10 nM...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90257-p
更新日期:1992-10-15 00:00:00
abstract::TJ6 is a novel protein which has immunosuppressive activity and may have a functional role in fetal allograft survival during pregnancy. Initial studies indicated that when mice were treated with an anti-TJ6 binding mAb early in pregnancy, the pregnancies were completely ablated and that TJ6 expression is enhanced dra...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1114
更新日期:1994-04-15 00:00:00
abstract::There are still questions about whether macrophage differentiation is predetermined or is induced in response to tissue microenvironments. C2D macrophage cells reside early in the macrophage lineage in vitro, but differentiate to a more mature phenotype after adoptive transfer to the peritoneal cavity (PEC-C2D). Since...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.012
更新日期:2011-01-01 00:00:00
abstract:AIMS:Non-enzymatic reaction of biomolecules leads to the formation of advanced glycation end products (AGEs). AGEs plays significant role in the pathophysiology of type 2 diabetes mellitus. Methylglyoxal (MG) is a highly reactive carbonyl compound which causes formation of early (ketoamines), intermediate (dicarbonyls)...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104062
更新日期:2020-05-01 00:00:00
abstract::In this study we assessed the viability of cultured human umbilical vein endothelial cells (HUVE) treated with bacterial lipopolysaccharide (LPS), recombinant human interleukin-1 (rhIL-1), or recombinant human tumor necrosis factor-alpha (rhTNF-alpha) during inhibition of RNA or protein synthesis. Cytotoxicity was det...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90222-0
更新日期:1989-03-01 00:00:00
abstract::This study provides evidence that both rat and mouse thymic and splenic T cells express significant levels of MHC class II glycoproteins (MHCII) in vivo. Derivation of rat and mouse chimeras revealed that a major source of MHCII on thymic T cells was acquired from radioresistant host APC. Expression of MHC on thymic T...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00577-4
更新日期:2002-07-01 00:00:00
abstract::Tumor necrosis factor (TNF) is a 17-kDa protein produced by endotoxin-stimulated macrophages. We have demonstrated that recombinant human TNF activates human macrophages to kill intracellular bacteria of the Mycobacterium avium complex (MAC) in a dose-related manner. TNF also primed macrophages to produce superoxide a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90243-i
更新日期:1992-10-15 00:00:00
abstract::The actions of interleukin-4 (IL-4) in vivo are likely to be positively influenced by the expression of membrane IL-4 receptors (mIL-4R) on target cells and negatively by the concentration of soluble IL-4 receptors (sIL-4R) in the extracellular environment. Inasmuch as the two forms of the mouse IL-4R are differential...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1181
更新日期:1997-09-15 00:00:00
abstract::Antigen-specific signal transduction leading to IL2 induction and secretion in the T cell line 171 is augmented by association of p56lck with CD4. Although no change in cytoplasmic calcium level ([Ca2+]i) was detectable during antigen-specific signal transduction of 171-CD4+ cells, IL2 induction was inhibited by FK506...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1255
更新日期:1994-10-01 00:00:00
abstract::Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, played an important role in immune-mediated diseases. The promoter region of MIF, which had functional polymorphisms, controlled MIF expression. MIF polymorphism was associated with many inflammatory diseases. But the association of MIF polymor...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.04.013
更新日期:2013-04-01 00:00:00
abstract::The precise role of the granular enzyme A (granzyme A), a serine protease, in the lytic process of cytotoxic T lymphocytes (CTL) is not clear. We have recently constructed a CTL line transfected with the antisense gene of granzyme A (a-GrA). These a-GrA CTL had lower GrA activity as well as decreased lytic activities,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0300
更新日期:1996-12-15 00:00:00
abstract::The mechanism of how superantigens function to activate cells has been linked to their ability to bind and cross-link the major histocompatibility complex class II (MHCII) molecule. Cells that lack the MHCII molecule also respond to superantigens, however, with much less efficiency. Therefore, the purpose of this stud...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1571
更新日期:1999-11-01 00:00:00
abstract::We tested the effects of salen manganese (Salen-Mn) complexes, which are scavengers of reactive oxygen species exhibiting superoxide dismutase and catalase activities on the rejection of and alloresponse to fully allogeneic skin grafts in mice. We showed that pre-transplant treatment of C57Bl/6 donor skin or of BALB/c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.06.009
更新日期:2006-06-01 00:00:00
abstract::The biological response modifier OK-432 has been shown both to exert the enhancement of several immunological activities and to have a direct anti-tumor effect. The present study examines the immunopotentiating effect of OK-432 on peripheral blood monocytes (PBM) derived from normal humans. Monocyte activation was ass...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90297-9
更新日期:1989-10-15 00:00:00
abstract::Mice infected with murine acquired immunodeficiency syndrome (MAIDS) virus developed lymphoadenopathy and profound immunodeficiency. Concomitantly the expression of endogenous mammary tumor virus (MTV) mRNA increased significantly, especially for the 1.7-kb 3' open reading frame (ORF) mRNA encoding MTV superantigen. B...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1326
更新日期:1998-08-01 00:00:00