Abstract:
:Differences in gender immune response have resulted in differences in immune protection and susceptibility to inflammatory diseases. Cultured peripheral blood mononuclear cells (PBMC) are widely used in immunomodulation studies, yet the influence of gender is usually not considered. We examined the effect of in vitro culture and phytohaemagglutinin (PHA) stimulation on PBMC lymphocyte subsets using flowcytometry. Full blood counts of whole blood showed higher levels of lymphocyte in male subjects. Lymphocyte subsets enumeration revealed higher NK cell counts in males and higher B cells in females. Cultured PBMC resulted in significant increases in B and total T cell percentages among females and NK cells among males. PHA stimulated significantly increased percentages of NK and total T cells in males and total activated T cells (CD69+) in females. Our results showed significant gender differences in lymphocyte subsets in cultured conditions. This may affect experimental outcome.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Abdullah M,Chai PS,Chong MY,Tohit ER,Ramasamy R,Pei CP,Vidyadaran Sdoi
10.1016/j.cellimm.2011.10.009subject
Has Abstractpub_date
2012-01-01 00:00:00pages
214-9issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(11)00270-Xjournal_volume
272pub_type
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journal_title:Cellular immunology
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journal_title:Cellular immunology
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pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章,评审
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更新日期:2016-08-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90309-5
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pub_type: 杂志文章
doi:10.1016/0008-8749(89)90190-1
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pub_type: 杂志文章
doi:10.1006/cimm.1999.1503
更新日期:1999-06-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/0008-8749(87)90304-2
更新日期:1987-10-01 00:00:00
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更新日期:2013-07-01 00:00:00
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