Regulation of antibody secretion by hybridoma cells. II. Mechanism of idiotype-induced suppression of antibody secretion by hybridoma cells.

Abstract:

:We have previously reported that antibody secretion by B.22 hybridoma cells can be suppressed in an MHC-restricted manner, by idiotype-specific T cells. It was shown that T cells of both helper and suppressor phenotypes are involved, and that the suppression is mediated by soluble factors. In the present paper, we have characterized the effects of T-cell-mediated suppression at the level of B.22 antibody mRNA expression and stability. Nuclear run-on analysis comparing suppressed and control B.22 cells indicates no change in the transcription rates of heavy and light chains. Northern blot analysis demonstrates that steady-state levels of heavy and light chain mRNAs are also unchanged. Thus, the suppression of antibody secretion by B.22 cells probably occurs at the levels of translation or secretion.

journal_name

Cell Immunol

journal_title

Cellular immunology

authors

Gulati P,Falck-Pedersen E,Siskind GW

doi

10.1016/0008-8749(89)90288-8

subject

Has Abstract

pub_date

1989-10-15 00:00:00

pages

276-82

issue

2

eissn

0008-8749

issn

1090-2163

pii

0008-8749(89)90288-8

journal_volume

123

pub_type

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