Abstract:
:To clarify the role of B cells in the establishment of T cell response against intracellular bacteria, B-cell-deficient (muMT-/-) mice were infected with an intracellular bacteria, Listeria monocytogenes, and T cell response against the bacteria was analyzed. On day 6 of primary Listeria infection, spleen T cells of the muMT-/- mice showed significantly lower levels of proliferative response and IFN-gamma production than those of normal infected mice after in vitro stimulation with listerial antigen. Even in the secondary Listeria infection after immunization with viable bacteria, spleen T cells of the muMT-/- mice proliferated and produced IFN-gamma against listerial antigen at significantly lower levels than those of normal immunized mice. These results demonstrate participation of B cells in priming of Listeria-specific T cells in vivo. However, B cells failed to present Listeria antigen to Listeria-specific T cells in vitro unless Listeria antigen was solubilized. Furthermore, transfer of immune serum from Listeria-infected normal mice failed to enhance the Listeria-specific T cell response of muMT-/- mice. The results indicate that B cells support the T cell response against intracellular bacteria through a mechanism other than their Ig production or antigen presentation function.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Matsuzaki G,Vordermeier HM,Hashimoto A,Nomoto K,Ivanyi Jdoi
10.1006/cimm.1999.1503subject
Has Abstractpub_date
1999-06-15 00:00:00pages
178-85issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(99)91503-4journal_volume
194pub_type
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journal_title:Cellular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1006/cimm.1997.1204
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doi:10.1006/cimm.1995.1180
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pub_type: 杂志文章
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pub_type: 杂志文章
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