Abstract:
:Cluster of differentiation ([CD]) 19 antigens are B-cell-specific molecules expressed on virtually all human cells of the B-lymphocyte lineage except plasma cells. We produced a new anti-CD19 monoclonal antibody (McAb), CLB-CD19, that was used to study the role of CD19 molecules in B-cell activation. Anti-CD19 McAb induced mobilization of free intracellular calcium ([Ca2+]i) in Daudi cells, but not in normal spleen or tonsillar B cells, for which crosslinking with a second anti-mouse Ig antibody was not required. Anti-CD19 McAb inhibited B-cell proliferation induced by anti-IgM coupled to Sepharose beads. This inhibitory effect was overcome by the addition of nonmitogenic concentrations of phorbol myristate acetate. Anti-CD19 McAb did not interfere with Staphylococcus aureus- or B-cell growth factor-induced B-cell proliferation. Anti-CD19 McAb inhibited T-cell-dependent polyclonal B-cell differentiation in pokeweed mitogen-, interleukin 2-, or anti-CD3-driven culture systems. Delayed addition studies showed that once differentiation of B cells was induced, CD19 molecules lost their regulating function. Taken together, our results indicate that CD19 molecules play a regulatory role in B-cell proliferation and differentiation.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
de Rie MA,Schumacher TN,van Schijndel GM,van Lier RA,Miedema Fdoi
10.1016/0008-8749(89)90385-7subject
Has Abstractpub_date
1989-02-01 00:00:00pages
368-81issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(89)90385-7journal_volume
118pub_type
杂志文章abstract::Senescence is characterized by several alterations in the immune system. Such modifications can be found in lymphoid organs as well as in the cellular components of the immune system. Several reports have suggested that immune dysfunction can affect both T and B cells, but T cells have been shown to be more susceptibl...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.01.010
更新日期:2009-01-01 00:00:00
abstract::Microglia are important immunoregulatory cells within the central nervous system (CNS). Viral infection of primary microglia and splenic macrophage clones revealed that both exhibited a heterogeneous, but relatively low, sensitivity to cytolysis mediated by CD8(+) cytotoxic T lymphocytes (CTL). The majority of clones ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1581
更新日期:1999-11-25 00:00:00
abstract::TE44, an H-2b-restricted, self-reactive T-cell line, did not produce autocrine-acting growth factors, neither after antigenic nor after mitogenic activation; they remained for their proliferation completely dependent on exogenously added IL-2. Administration of IL-4, which poorly promotes growth by itself, resulted in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90163-l
更新日期:1990-10-01 00:00:00
abstract::Peripheral blood lymphocytes (PBL) from patients with herpes simplex virus (HSV)-1 recurrences in the cornea only (Group I) exhibited reduced lysis of HSV-1-infected targets compared to PBL from patients with oral-facial and corneal HSV recurrences (Group II). The cytotoxic lymphocytes appeared to belong to a subpopul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90305-8
更新日期:1984-02-01 00:00:00
abstract::Although it is well established that the processes of cellular proliferation and apoptosis are linked, the role of cell cycle regulators in T cell responses in vivo is not well understood. In recent years, tumor suppressor molecule p19(ARF) has emerged as a key cell cycle regulator important in cellular apoptosis agai...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00597-x
更新日期:2002-10-01 00:00:00
abstract::Cyclosporin A (CsA) was tested for its modulatory effects on the mIgM-mediated signaling of G0*-associated increases in class II MHC expression, G1-related RNA synthesis, and S phase-related DNA synthesis in human B cells. While CsA at concentrations as low as 10-100 ng/ml could completely ablate anti-IgM-induced DNA ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90213-9
更新日期:1992-04-01 00:00:00
abstract::Transduction with recombinant, replication-defective adenoviral (rAd) vectors encoding a transgene is an efficient method for gene transfer into human dendritic cells (DCs). Livin is a good candidate for cancer immunotherapy since it is overexpressed in most common human cancers, poorly expressed in most normal adult ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.06.003
更新日期:2015-09-01 00:00:00
abstract::Like the thymus, the bursa of Fabricius is a site of massive lymphopoiesis accompanied by cell death in vivo. In the present study we have, therefore, examined whether chicken bursa and thymus cells exhibit apoptosis. Bursa and thymus cells from SC chickens, 4-10 weeks of age, were incubated for 8-24 hr with various r...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1058
更新日期:1993-03-01 00:00:00
abstract::Mature thymocytes are usually defined and separated from other less mature thymocytes on the basis of their mutually exclusive expression of either CD4 or CD8. However, such murine "single positives" include a subpopulation of immature cells with properties resembling CD4- CD8- thymocytes or CD4+ CD8+ cortical blasts....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90042-1
更新日期:1988-05-01 00:00:00
abstract::The NK-susceptibility of dystrophic mouse myoblast cells was investigated. Spleen cells from 8- to 10-week-old normal (+/+) and dystrophic (dy2J/dy2J) male C57BL/6J mice were fractionated on Percoll density gradients and the cells at each density interface were incubated with either 51Cr-labeled YAC-1 or myoblast cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90003-1
更新日期:1986-06-01 00:00:00
abstract::Transforming growth factor beta1 (TGFbeta1) is a potent negative immunoregulatory molecule. We have previously shown that the autoimmune-mediated weaning-age lethality of Tgfb1-/- mice is reversed upon genetic combination with Scid or Rag null alleles. Here, we show that elimination of T but not B cells is sufficient ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.02.004
更新日期:2004-11-01 00:00:00
abstract::We showed previously that the expressions of various src family protein tyrosine kinases (PTKs) were induced independently during the monocytic differentiation of HL-60 cells. The role of PTKs was further assessed in the present study by investigating the effects of PTK inhibitors on the differentiation. It was demons...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90196-v
更新日期:1992-04-01 00:00:00
abstract::The effect of the addition of dialyzable leukocyte extract (DLE)(transfer factor) to tuberculostatic drugs in the treatment of superinfected fistulating tuberculosis of bones and joints was evaluated in a controlled study. Eleven patients whose disease had persisted for a mean of 20 +/- 4.8 years and had proved to be ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90091-1
更新日期:1984-03-01 00:00:00
abstract::B-1a cells represent a distinct B cell population with unique phenotype, self-renewing capacity and restricted Igμ repertoire. They primarily locate in body cavity and also exist in spleen. The different subpopulations of B-1a cells are heavily affected by local environment. Our previous studies revealed that MARVEL-d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104100
更新日期:2020-06-01 00:00:00
abstract::Development of contact hypersensitivity in mice depends on the migration of Langerhans cells from the epidermis to regional lymph nodes. Since ICAM-1 and LFA-1 play important roles in leukocyte migration, we sought to determine whether in vivo administration of anti-ICAM-1 and anti-LFA-1 antibodies would inhibit conta...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1285
更新日期:1994-10-15 00:00:00
abstract::The lymphokine IL-4 is a potent enhancer of anti-IgM-induced B cell proliferation. Although the mechanism of this enhancement is not known, a commonly held view suggests that IL-4 acts together with anti-IgM as a costimulating factor for the activation of a subpopulation of B cells. To evaluate this hypothesis we exam...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90327-8
更新日期:1991-04-15 00:00:00
abstract:OBJECTIVES:Piperine, a main component of Piper species, is a plant alkaloid with a long history of medical use in a variety of inflammatory disorders like rheumatoid arthritis. Due to side effects in current treatment modalities of rheumatoid arthritis, the interest in alternative, well tolerated anti-inflammatory reme...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.07.004
更新日期:2013-07-01 00:00:00
abstract::We have recently established a new animal model for primary Sjögren's syndrome in NFS/sld mutant mice thymectomized 3 days after birth (3dTX) bearing an autosomal recessive gene with sublingual gland differentiation arrest. In this study, we analyze developing mechanisms of experimental autoimmune sialadenitis (EAS) i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0196
更新日期:1996-08-01 00:00:00
abstract::Coxsackievirus group B type 3 (CVB3) induces myocarditis in male Balb/c mice but produces little cardiac injury in females. Males develop cytolytic T lymphocytes (CTL) reactive to heart antigens which primarily cause the inflammation and cardiac injury observed in the disease. Infected female mice lack this CTL respon...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90271-6
更新日期:1986-03-01 00:00:00
abstract::We investigated the role of IL-12 in regulating IL-2 and IFN-gamma production in primary culture of human T cells. Addition of neutralizing antiserum against the 40-kDa subunit of IL-12 to PHA-stimulated PBMC markedly reduced both IFN-gamma protein production and mRNA accumulation and stability. Moreover, concurrent t...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1303
更新日期:1994-12-01 00:00:00
abstract::Macrophages can be divided into two groups as M1 and M2 phenotype. Our results and other groups revealed that IFN-γ can up-regulate the IDO expression and differentiate THP-1 cells to M1 phenotype. Therefore we hypothesized that IDO may play potential roles in macrophage differentiation. Interesting, our results indic...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2014.02.005
更新日期:2014-05-01 00:00:00
abstract::Expression of certain autologous lymphocyte-activating antigenic determinants on the developing embryo is known to provide a stimulus for maternal anti-fetal autoproliferative responses. If left unregulated these responses could exert negative influences on the reproductive process by converting to autoaggressive form...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90173-1
更新日期:1989-04-15 00:00:00
abstract::We examined the effects of a rare sugar, D-allose, which is 6-carbon monosaccharide, on endocytosis and T cell stimulation by dendritic cells (DCs). The endocytosis of BCG-anti-BCG immune complexes by DCs markedly decreased in D-allose-containing medium. Co-culture with T cells (mixed leukocyte reaction, MLR) of DCs, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.015
更新日期:2011-01-01 00:00:00
abstract::We previously reported that T cell proliferation in response to a primary signal through the T cell receptor (TCR) and a costimulatory signal via the CD28 molecule is impaired in healthy, aged mice. Here we extend these studies to examine factors which may be involved in this defect in T cells from aged mice. To deter...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0144
更新日期:1996-05-25 00:00:00
abstract::Viruses are related to the etiology of periodontitis. However, the role of viruses on Th17 cells infiltration in periodontitis lesions is unknown. Therefore, we examined the effects of TLR3 ligand on CCL20, which is related to Th17 cells migration, production in human gingival fibroblasts (HGFs). Polyinosinic-polycyti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.05.005
更新日期:2013-05-01 00:00:00
abstract::Neoplastic thymocytes from rat thymic lymphoma-leukemias induced by the rat-adapted Gross leukemia virus (RAGV) were analyzed for a variety of differentiation markers. The neoplasms from individual rats all expressed the antigenic phenotype MP+, W3/13+, Thy-1+, RT-1+, RT-7+, W3/25-. However, approximately two-thirds o...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90089-9
更新日期:1985-08-01 00:00:00
abstract::We have established murine T-cell clones which respond to allotypic and species-specific determinants found on chicken erythrocytes (cRBC). Their relative antigen specificities were determined by assessing lymphokine production and proliferation in response to syngeneic spleen cells and cRBC obtained from chickens hom...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90406-5
更新日期:1986-02-01 00:00:00
abstract::The purpose of the present study was to compare the phenotype of tissue macrophages with that of their precursors in the bone marrow and blood. The phenotype was determined on the basis of the quantitative binding of monoclonal antibodies to cell-surface antigens (antigen F4/80, complement receptor III, Fc receptor II...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90085-2
更新日期:1987-04-01 00:00:00
abstract::The candidacidal activity (CA) of peritoneal cells (PC) in vitro was used as a measure of nonspecific microbicidal activity of phagocytes after intraperitoneal injection of mice with different adjuvants. Dilutions of PC were incubated with constant numbers of C. parapsilosis in a 96-well culture plate. The PC number c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90164-9
更新日期:1985-01-01 00:00:00
abstract::The impact of BCR:CD21 co-engagement on B cell expression of molecules critical for T cell activation was investigated with receptor-specific mAbs conjugated to high MW dextran as stimulatory ligands. In the absence of IL-4, BCR:CD21 co-ligation augmented BCR-triggered CD86 only under conditions of very low BCR ligand...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00512-9
更新日期:2002-03-01 00:00:00