Abstract:
:The NK-susceptibility of dystrophic mouse myoblast cells was investigated. Spleen cells from 8- to 10-week-old normal (+/+) and dystrophic (dy2J/dy2J) male C57BL/6J mice were fractionated on Percoll density gradients and the cells at each density interface were incubated with either 51Cr-labeled YAC-1 or myoblast cells in a 6 hr 51Cr-release assay. Myoblast target cells were obtained from either heterozygous (+/dy2J) or homozygous (dy2J/dy2J) muscle cultures or a transformed tetraploid myoblast line (M14D2). The data indicate that the interface between the 50 and 60% (1.060-1.075 g/ml) Percoll density fractions of spleen cells from either normal or dystrophic mice contains the largest proportion of asialo GM-1 positive and NK-1 positive cells displaying NK activity. Myoblast cells from either heterozygous (phenotypically normal) or homozygous dystrophic mice were not significantly different in susceptibility to NK-mediated lysis by Percoll enriched normal or dystrophic mouse NK cells. However, dystrophic mouse spleen cells had the highest NK activity against both myoblast targets as compared with normal mouse spleen cells. The transformed myoblast cell line, M14D2, was significantly less susceptible to NK-mediated lysis by dystrophic mouse spleen cells when compared with freshly cultured myoblast target cells. Target cell binding studies revealed that conjugate forming cells from the 50% Percoll density interface of dystrophic mouse spleen cells were approximately twofold greater than that of normal mouse spleen cells against either heterozygous or homozygous dystrophic mouse myoblast targets. Cold target inhibition studies revealed that the natural killing of dystrophic mouse myoblast cells was due to a YAC-1 reactive NK cell. Breeding experiments between C57BL/6J homozygous "beige" (bgJ/bgJ) mutant mice and dystrophic (dy2J/dy2J) mice produced beige/dystrophic hybrid mice which displayed clinical symptoms of the dystrophy process by 3 to 4 weeks of age. Spleen cells from these hybrid mice showed no significant differences in NK activity against YAC-1 target cells when compared with homozygous beige mice. Taken together, these results demonstrate the first reported evidence that murine myoblasts are susceptible to NK-mediated lysis. In addition, the data indicate that although dystrophic mouse NK cells recognize myoblast cells as targets, the NK cell studies with the beige/dystrophic hybrid mice do not indicate a direct in vivo role for NK cells in the dystrophy process.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Semple JW,Szewczuk MRdoi
10.1016/0008-8749(86)90003-1subject
Has Abstractpub_date
1986-06-01 00:00:00pages
20-33issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(86)90003-1journal_volume
100pub_type
杂志文章abstract::Class II major histocompatibility antigens (Ia) play a major role in regulating T-B cell interactions; therefore, regulation of the amount of Ia on B cells may be an important point of control in the immune response. Mitogens in human and murine systems have been reported to increase Ia expression on B cells, and in t...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90108-6
更新日期:1988-02-01 00:00:00
abstract::The actions of interleukin-4 (IL-4) in vivo are likely to be positively influenced by the expression of membrane IL-4 receptors (mIL-4R) on target cells and negatively by the concentration of soluble IL-4 receptors (sIL-4R) in the extracellular environment. Inasmuch as the two forms of the mouse IL-4R are differential...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1181
更新日期:1997-09-15 00:00:00
abstract::A low-virulence, agerminative strain of Candida albicans (PCA-2) is able to confer a high degree of nonspecific protection against subsequent challenge with highly virulent microorganisms in mice. In an attempt to better define the effect of PCA-2 vaccination on the immune system and the nature of the mechanisms invol...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90135-4
更新日期:1989-12-01 00:00:00
abstract::PNK-E and G7 mAbs regulate porcine NK and ADCC activities by binding to distinct NK function-associated trigger molecules on porcine NK cells. Previous work demonstrates that PNK-E mAb binds to a 205-kDa tetrameric molecule composed of two 47-kDa peptides and two 50-kDa peptides and G7 mAb binds to a distinct 40-kDa h...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1026
更新日期:1993-02-01 00:00:00
abstract::To determine the contribution of B cells to brain myelin injury in Semliki Forest Virus (SFV) encephalomyelitis, normal C57BL/6 (B6) and B-cell-deficient (C57BL/6-tm1Cgn) B6 mice were infected with SFV. The peak of clinical disease, i.e., the time at which the greatest proportions of mice had moderate to severe clinic...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1613
更新日期:2000-02-25 00:00:00
abstract::The expression and function of surface immunoglobulin (sIg) receptors was analyzed using antigen-specific monoclonal B cells and T cells. When B cells were incubated with antigen (hapten carrier), hapten-specific sIg receptor expression was greatly reduced. The specific antigen-presentation activity of the B cells was...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90291-2
更新日期:1988-03-01 00:00:00
abstract::B-1a cells represent a distinct B cell population with unique phenotype, self-renewing capacity and restricted Igμ repertoire. They primarily locate in body cavity and also exist in spleen. The different subpopulations of B-1a cells are heavily affected by local environment. Our previous studies revealed that MARVEL-d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104100
更新日期:2020-06-01 00:00:00
abstract::Although engagement of MHC class II molecules on human monocytes triggers various cellular events, their possible role in monocyte death is yet unknown. We demonstrate that ligation of MHC class II on primary monocytes induces a rapid cell death that has all the characteristics of monocyte apoptosis, does not require ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1428
更新日期:1999-02-25 00:00:00
abstract::Mice that differ at Igh loci and the reagents that permit resolution of such distinctions are powerful genetic tools for studying B cell heterogeneity. In this report, IgM-allotype-specific ELISA revealed that two of three sources of mice designated as BALB.nude express the Igh-6b (IgMb) allotype characteristic of C57...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1125
更新日期:1993-05-01 00:00:00
abstract::We investigated the anti-Fas antibody-induced cell death in two different types of human cell lines, U937 and B104. IFN-gamma increased the surface expression of Fas antigen and susceptibility to anti-Fas Ab-induced cell death of B104 and U937 cells. Anti-Fas Ab-induced death of U937 and B104 cells required neither a ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1016
更新日期:1994-01-01 00:00:00
abstract::CXCL10 production is a critical step in limiting mycobacterial infection. Although induction of this chemokine by mycobacteria in epithelial cells has been reported, it is still unclear how CXCL10 is regulated in Mycobacterium bovis BCG-infected epithelial cells. In this study, we demonstrate that phosphatidylinoditol...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.12.002
更新日期:2009-01-01 00:00:00
abstract::One of the auto-antigens aberrantly targeted in Multiple sclerosis is myelin basic protein (MBP). In this study, chronic progressive multiple sclerosis (CPMS) patients receiving the experimental drug MBP8298, on a compassionate care trial, were examined before and after high dose peptide treatment for their circulatin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.11.004
更新日期:2012-11-01 00:00:00
abstract::The precise role of the granular enzyme A (granzyme A), a serine protease, in the lytic process of cytotoxic T lymphocytes (CTL) is not clear. We have recently constructed a CTL line transfected with the antisense gene of granzyme A (a-GrA). These a-GrA CTL had lower GrA activity as well as decreased lytic activities,...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0300
更新日期:1996-12-15 00:00:00
abstract::In the present study we show that the opioid peptide beta-endorphin (beta-End) enhances Ia expression on murine B cells in cultures of unseparated spleen cells, mediated by low concentrations of IL-4 in the absence of antigenic or mitogenic stimulation. Since this effect was not found with purified B cells and no enha...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1146
更新日期:1993-06-01 00:00:00
abstract::We examined the effects of a rare sugar, D-allose, which is 6-carbon monosaccharide, on endocytosis and T cell stimulation by dendritic cells (DCs). The endocytosis of BCG-anti-BCG immune complexes by DCs markedly decreased in D-allose-containing medium. Co-culture with T cells (mixed leukocyte reaction, MLR) of DCs, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.015
更新日期:2011-01-01 00:00:00
abstract::Neoplastic thymocytes from rat thymic lymphoma-leukemias induced by the rat-adapted Gross leukemia virus (RAGV) were analyzed for a variety of differentiation markers. The neoplasms from individual rats all expressed the antigenic phenotype MP+, W3/13+, Thy-1+, RT-1+, RT-7+, W3/25-. However, approximately two-thirds o...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90089-9
更新日期:1985-08-01 00:00:00
abstract::The effects of oral administration of methylated bovine serum albumin (mBSA) on antigen-induced arthritis (AIA) in rats that was induced by immunization with mBSA followed by the intraarticular injection of the same antigen were investigated. When mBSA was orally given to animals before immunization with the antigen, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1098
更新日期:1995-06-01 00:00:00
abstract::The lpr mutation in mice results in premature termination of transcription of the gene encoding the apoptosis-signaling receptor Fas. As a result, lpr mice develop severe lymphoproliferation and lupus-like autoantibodies. Growing evidence suggests that the lpr mutation is "leaky" and that low levels of Fas are express...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1290
更新日期:1998-05-25 00:00:00
abstract::The "NK-triggering-antigen regulator" (Nktar) gene is a locus identified in the C57BL/6 genome which regulates the ability of unlabeled activated Con A blasts to compete for recognition of labeled syngeneic Con A blasts by BALB/c NK cells. Linkage analysis on Con A blasts from (BALB/c x CByB6F1) N2 backcross progeny f...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1624
更新日期:2000-03-15 00:00:00
abstract::Curcumin has commonly been used for the treatment of various allergic diseases. However, its precise anti-allergic rhinitis effect and mechanism remain unknown. In the present study, the effect of curcumin on allergic responses in ovalbumin (OVA)-induced allergic rhinitis mouse was investigated. We explored the effect...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.09.010
更新日期:2015-11-01 00:00:00
abstract::Immunosuppression is a well-established risk factor for Visceral Leishmaniasis. Post-immunosuppression leishmaniasis is characterized by an increase of parasite burden, hematopoietic disorders and unusual clinical manifestations. Although there are many reports on bone marrow findings in VL, less is known about the re...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.10.016
更新日期:2018-04-01 00:00:00
abstract::Neuroendocrine hormones such as growth hormone (GH) and prolactin (PRL) have been demonstrated to accelerate the recovery of the immune response after chemotherapy and bone marrow transplantation and to enhance the restoration of immunity in individuals infected with HIV and in normal individuals with compromised immu...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2007.12.003
更新日期:2008-03-01 00:00:00
abstract::Murine histocompatibility antigens were solubilized from the spleens and lungs of C57BL/6 (H-2b) animals with hypertonic salt (3 M KC1). Aggregate-free soluble antigens were incubated with nonadherent lymph node cells from BALB/c (H-2d) mice for 18 hr prior to their use as responder cells in the mixed-lymphocyte react...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90281-2
更新日期:1983-06-01 00:00:00
abstract::We have investigated the ability of long-term cultured T helper (Th) cell lines to help an in vivo cytotoxic T lymphocyte (CTL) response to non-H-2 alloantigens (minor antigens). Th cell lines specific for various single or undefined minor antigens were selected by regular restimulation with antigen in vitro. They wer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90309-5
更新日期:1984-02-01 00:00:00
abstract::Coxsackievirus group B type 3 (CVB3) induces myocarditis in male Balb/c mice but produces little cardiac injury in females. Males develop cytolytic T lymphocytes (CTL) reactive to heart antigens which primarily cause the inflammation and cardiac injury observed in the disease. Infected female mice lack this CTL respon...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90271-6
更新日期:1986-03-01 00:00:00
abstract::The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90302-4
更新日期:1988-04-01 00:00:00
abstract::The development of B cell tolerance is believed to involve negative signaling to B cells derived from the binding of antigen to the B cell surface immunoglobulin. B cell lines that receive negative signals may provide useful models for studying the mechanisms of B cell tolerance. We have established a human B lymphoma...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1314
更新日期:1994-12-01 00:00:00
abstract::We recently reported that a cloned murine cell line with "natural killer (NK)-like" cytolytic function and prominent cytoplasmic granules also expressed large numbers of plasma membrane receptors (Fc epsilon R) which bound mouse immunoglobulin E (IgE) with high affinity (S.J. Galli et al., 1982, Nature (London) 298, 2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90353-3
更新日期:1985-11-01 00:00:00
abstract::In the present work we have studied the occurrence and the characteristics of human B lymphocytes which are capable of spontaneous immunoglobulin (Ig) production in short-term cultures. It was found that this type of B cells secreted predominantly IgG and were present in the circulation as well as in the lymphoid tiss...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90280-7
更新日期:1986-03-01 00:00:00
abstract::The role of class II antigens for B-cell activation was analyzed using purified human B cells and anti-class II monoclonal antibodies. The stimulation of purified B cells with Staphylococcus aureus Cowan I induced proliferation and differentiation into immunoglobulin-producing cells in the presence of interleukin-1 an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90295-x
更新日期:1988-04-01 00:00:00