Abstract:
:In the present work we have studied the occurrence and the characteristics of human B lymphocytes which are capable of spontaneous immunoglobulin (Ig) production in short-term cultures. It was found that this type of B cells secreted predominantly IgG and were present in the circulation as well as in the lymphoid tissue (tonsil and lymph node) of normal subjects. Tissular and circulating cell subsets exhibited many similarities: These cells produced Ig during a 3-day culture period without apparent need for T cells or monocytes. Protein and DNA synthesis were required for Ig secretion to occur. In Percoll fractionation experiments these cells showed low density, as they were mainly collected in the 42.5-45% Percoll fractions. These subsets consisted of cells considerably larger in size than the majority of B lymphocytes, as determined by Ig sedimentation. They were commonly defined as SmIg- Ia+ B cells by panning fractionation techniques. All these common characteristics suggest that these B cells have reached an advanced stage of maturation in vivo in both circulation and lymphoid tissue. Further surface marker analysis demonstrated that tissular but not circulating B-cell subset reacted with peanut agglutinin and the BA-2 monoclonal antibody which probably indicates their germinal center origin.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Sen ML,Garcia-Alonso A,Brieva JAdoi
10.1016/0008-8749(86)90280-7subject
Has Abstractpub_date
1986-03-01 00:00:00pages
200-10issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(86)90280-7journal_volume
98pub_type
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90372-6
更新日期:1983-03-01 00:00:00
abstract::Non-obese diabetic (NOD) mice develop spontaneous T-cell responses against pancreatic beta-cells, leading to islet cell destruction and diabetes. Despite high genetic similarity, non-obese resistant (NOR) mice do not develop diabetes. We show here that spleen cells of both NOD and NOR mice respond to the islet cell an...
journal_title:Cellular immunology
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journal_title:Cellular immunology
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journal_title:Cellular immunology
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abstract::Because of the massive cytokine response elicited by superantigen exposure, it has been suggested that superantigens may act as adjuvants to boost conventional antigen responses. However, most previous studies have shown that in vivo exposure to superantigen suppressed subsequent T cell responses. Here we analyzed the...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.1077
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abstract::Investigation into the mechanism of action of vaccine adjuvants provides opportunities to define basic immune principles underlying the induction of strong immune responses and insights useful for the rational development of subunit vaccines. A novel HIV vaccine composed of plasmid DNA-encoding p55 gag formulated with...
journal_title:Cellular immunology
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journal_title:Cellular immunology
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doi:10.1016/0008-8749(89)90376-6
更新日期:1989-02-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
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更新日期:1986-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章,评审
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更新日期:1990-10-01 00:00:00
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0271
更新日期:1996-11-01 00:00:00
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journal_title:Cellular immunology
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doi:10.1016/0008-8749(84)90242-9
更新日期:1984-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90289-3
更新日期:1986-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-04-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
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更新日期:1987-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1024
更新日期:1994-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2007.12.003
更新日期:2008-03-01 00:00:00
abstract::Although it is known that IGF-1 increases T lymphocyte proliferation, the regulation of its receptor expression during the process has not been defined. Consequently the regulation of IGF-1R expression by IGF-1 and the activation events in human blood T lymphocytes and the Jurkat T cell line were now investigated. IGF...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1237
更新日期:1998-02-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90294-l
更新日期:1991-07-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1016
更新日期:1994-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90050-x
更新日期:1986-07-01 00:00:00
abstract::Recognition of lipopolysaccharide (LPS) by Toll-like receptor 4 (TLR4) activates two district proinflammatory signaling pathway and initiates LPS internalization. To investigate roles of LPS internalization, a traditionally regarded metabolic pathway for LPS, in regulation of these two pathways, three internalization ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.12.007
更新日期:2012-01-01 00:00:00
abstract::CD20 is expressed strictly by B-cells and is ubiquitously expressed at high surface densities of malignant human B-cells. This suggests that CD20 may be a tumor target for immunotherapy of B-cell lymphomas. Rituximab, a chimeric monoclonal antibody directed against CD20, has been demonstrated to be an effective treatm...
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pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.05.005
更新日期:2006-02-01 00:00:00