Runx-dependent regulation of G-protein gamma3 expression in T-cells.

Abstract:

:Heterotrimeric G-proteins control diverse biological processes by conveying signals from seven-transmembrane receptors to intracellular effectors. Although their signaling roles were originally ascribed to their GTP-bound alpha-subunits, more recent evidence points to the equally active roles played by their betagamma-dimers. To elucidate the individual contributions of their gamma-subtypes, we used a gene targeting approach to show that mice lacking the gamma3-subtype display a defective T-cell dependent immune response. To identify the cellular basis for this defect, we demonstrated that gamma3-mRNA is strongly induced in activated CD4+ T-cells. To determine the mechanism for this regulated expression, we used several strategies to identify the importance of a Runx consensus sequence element in the first intron of the gamma3 gene and the Runx1 protein. Overall, these data provide the first genetic evidence for the tight regulation and involvement of the G protein gamma3-subtype in mounting an effective immune response in mice.

journal_name

Cell Immunol

journal_title

Cellular immunology

authors

Dubeykovskiy A,McWhinney C,Robishaw JD

doi

10.1016/j.cellimm.2006.06.008

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

86-95

issue

2

eissn

0008-8749

issn

1090-2163

pii

S0008-8749(06)00117-1

journal_volume

240

pub_type

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