Abstract:
:We investigated the role of IL-12 in regulating IL-2 and IFN-gamma production in primary culture of human T cells. Addition of neutralizing antiserum against the 40-kDa subunit of IL-12 to PHA-stimulated PBMC markedly reduced both IFN-gamma protein production and mRNA accumulation and stability. Moreover, concurrent treatment of partially purified T cells (> 90% CD3+) with PHA and rIL-12 selectively enhanced IFN-gamma mRNA stability and protein production, while IL-2 protein and mRNA levels were unaffected. These studies also show that IFN-gamma and IL-2 mRNA stability are temporally dissociated during the course of T cell activation, and we propose that this dissociation may be mediated through the production of IL-12. The effect of IL-12 on modulation of IFN-gamma mRNA turnover is not associated with detectable changes in either the levels or affinity of cytoplasmic RNA-binding proteins capable of recognizing AU-rich sequences in the 3'UTR of IFN-gamma mRNA.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Nagy E,Buhlmann JE,Henics T,Waugh M,Rigby WFdoi
10.1006/cimm.1994.1303subject
Has Abstractpub_date
1994-12-01 00:00:00pages
140-51issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(84)71303-7journal_volume
159pub_type
杂志文章abstract::The administration of low-density lipoproteins from chicken egg yolk (EyLDL) to mice induced T-dependent antibodies against bromelain-treated mouse erythrocytes (BrMRBC). The anti-BrMRBC antibodies produced antigen specifically by EyLDL appear to be identical to the antibodies produced polyclonally by lipopolysacchari...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90195-5
更新日期:1988-09-01 00:00:00
abstract::Anti-TNF-α therapies have been applied in RA treatment, but the regulatory effect of the drug on immune system is not clear. In this study, we included 33 active RA patients and divided them into two groups. One group received anti-TNF-α mAb+methotrexate for 24 weeks, the other group got placebo+methotrexate for the f...
journal_title:Cellular immunology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.cellimm.2012.09.001
更新日期:2012-09-01 00:00:00
abstract::Thirty-two cord blood samples taken after caesarean section or vaginal delivery and concurrent venous blood samples obtained from normal adult controls were evaluated using monoclonal antibodies. The percentage of circulating pan-T-cell+ lymphocytes was significantly lower in cord blood (46%) compared with adult contr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90069-3
更新日期:1985-04-15 00:00:00
abstract::The functional specificity of three types of macrophage chemotactic factors ( MCFs ), -a, -b, and -c, from purified protein derivative of tuberculin (PPD)-induced delayed hypersensitivity reaction (DHR) skin sites on guinea pigs, was analyzed using macrophage cell line cells, M1, established from myeloid leukemia cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90354-x
更新日期:1984-06-01 00:00:00
abstract::Severe aplastic anemia (SAA) is a syndrome of severe bone marrow failure with high mortality. Our previous studies have demonstrated that both immature and activated DC1 increased in the bone marrow of SAA patients, and the balance of DC1 subsets shifted the stable form to active one, which might promote Th0 cells to ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.09.007
更新日期:2013-09-01 00:00:00
abstract::PICOT (PKC-interacting cousin of thioredoxin) consists of one thioredoxin homology domain in the N-terminal and two tandem PICOT homology domains in the C-terminal. PICOT specifically interacts with protein kinase C theta (PKC-theta) via its thioredoxin homology domain and acts as an important modulator of T cell rece...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.04.005
更新日期:2008-01-01 00:00:00
abstract::Accumulating evidence indicates that inflammatory signals required for maximizing effector T cell generation have opposing effects on the development of memory T cell precursors. Toll-like receptor (TLR)2, and TLR9 significantly contribute to the inflammatory milieu and therefore in this study we examined whether the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.12.009
更新日期:2016-04-01 00:00:00
abstract::TJ6 is a novel protein which has immunosuppressive activity and may have a functional role in fetal allograft survival during pregnancy. Initial studies indicated that when mice were treated with an anti-TJ6 binding mAb early in pregnancy, the pregnancies were completely ablated and that TJ6 expression is enhanced dra...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1114
更新日期:1994-04-15 00:00:00
abstract::A mouse IgG1 monoclonal antibody ED12F8C10 (C10) binds a constant percentage of peripheral blood neutrophils in the same individual when studied over time, defining a distinct subset of neutrophils in all normal individuals studied to date. Bone marrow studies confirm that the heterogeneity is present to the same degr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90010-9
更新日期:1991-01-01 00:00:00
abstract::The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90302-4
更新日期:1988-04-01 00:00:00
abstract::Al adjuvants are used in vaccines to increase the immune response. NTPDase and AChE play a pivotal role and act in the regulation of the immune system. The effect of Al exposure in vitro and in vivo on NTPDase and AChE activities in the lymphocytes of rats was determined. In vitro, ATP hydrolysis was decreased by 20.4...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.08.001
更新日期:2010-01-01 00:00:00
abstract::The kinetics of viability of lymph node and spleen cells of mice genetically selected for "high" or "low" in vitro lymphocyte responsiveness to PHA were studied in PHA or PPD-stimulated short-term cultures. Lo/PHA cells were found to be less viable than Hi/PHA cells in unstimulated control cultures. PHA improved the v...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90008-4
更新日期:1983-04-01 00:00:00
abstract::Previous studies established that low in vitro temperatures (27 degrees C, termed nonpermissive) suppressed murine primary thymus-dependent (TD), but not primary thymus-independent (TI) or secondary TD, antibody responses. Suppression was rescued by the addition of oleic acid (18:1), recombinant interleukin (rIL)-2 an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1265
更新日期:1993-11-01 00:00:00
abstract::Cluster of differentiation ([CD]) 19 antigens are B-cell-specific molecules expressed on virtually all human cells of the B-lymphocyte lineage except plasma cells. We produced a new anti-CD19 monoclonal antibody (McAb), CLB-CD19, that was used to study the role of CD19 molecules in B-cell activation. Anti-CD19 McAb in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90385-7
更新日期:1989-02-01 00:00:00
abstract::The role of lymphostromal complexes in T-cell differentiation is far from elucidated, mainly because a clear association of a particular stromal cell type with a distinct thymocyte subset has never been identified. Using an in vitro system, detecting the adherence of thymocytes to a thymic medullary epithelial cell li...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90307-d
更新日期:1990-03-01 00:00:00
abstract::Evidence is presented that rat serosal mast cells and cells of the rat basophilic leukemia line, RBL-1, each contain a calcium-activated, phospholipid-dependent protein kinase. The enzymes are very similar in their activation requirements to the calcium-dependent enzymes termed protein kinase Cs in brain. The enzyme i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90098-5
更新日期:1986-12-01 00:00:00
abstract::Acting through CD14 and TLR4/MD-2, lipopolysaccharide (LPS) triggers strong pro-inflammatory activation of macrophages, which, if not appropriately controlled, may lead to lethal septic shock. Therefore, numerous mechanisms of negative regulation of responses to LPS exist, but whether they include down-regulation of L...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.11.008
更新日期:2017-02-01 00:00:00
abstract::Immunocytochemical staining procedures were combined with computer-aided image analysis to quantitate the relative intracellular production of tumor necrosis factor-alpha (TNF) within individual macrophages. Optimal conditions for time and methods for the activation of TNF production, fixation of cells for optimal imm...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90278-y
更新日期:1990-10-15 00:00:00
abstract::Purified monomorphic monoclonal antibodies against Class I and Class II antigens in the inhibition of in vitro allogenic response were assayed. As expected, anti-Class II antibodies are highly inhibitory when used in concentrations greater than 5 micrograms/ml in MLRI and 50 micrograms/ml in MLRII. Surprisingly, anti-...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90078-3
更新日期:1983-07-15 00:00:00
abstract::The actions of interleukin-4 (IL-4) in vivo are likely to be positively influenced by the expression of membrane IL-4 receptors (mIL-4R) on target cells and negatively by the concentration of soluble IL-4 receptors (sIL-4R) in the extracellular environment. Inasmuch as the two forms of the mouse IL-4R are differential...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1181
更新日期:1997-09-15 00:00:00
abstract::Human skin contains epidermal Langerhans cells (LCs) and dermal dendritic cells (DCs) that are key players in induction of adaptive immunity upon infection. After major burn injury, suppressed adaptive immunity has been observed in patients. Here we demonstrate that burn injury affects adaptive immunity by altering bo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.01.007
更新日期:2011-01-01 00:00:00
abstract::Chemotactic migration of T lymphocytes and neutrophil granulocytes within a three-dimensional collagen matrix is distinct from spontaneous, matrix-induced migration concerning dynamic parameters and regulatory intracellular signaling. Both spontaneous T lymphocyte locomotion and stromal-cell-derived factor-1 (SDF-1)-i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1605
更新日期:2000-02-01 00:00:00
abstract::Neoplastic thymocytes from rat thymic lymphoma-leukemias induced by the rat-adapted Gross leukemia virus (RAGV) were analyzed for a variety of differentiation markers. The neoplasms from individual rats all expressed the antigenic phenotype MP+, W3/13+, Thy-1+, RT-1+, RT-7+, W3/25-. However, approximately two-thirds o...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90089-9
更新日期:1985-08-01 00:00:00
abstract::We previously demonstrated that injection of a high dose (4 X 10(9] of sheep erythrocytes (SRBC) into C57BL/6 mice results in the generation of splenic B cells (plastic nonadherent, Thy-1- and Ig+) which, when transferred to normal syngeneic recipients, subsequently induce antigen-specific suppressor T cells to suppre...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90242-9
更新日期:1984-01-01 00:00:00
abstract::Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus transformed B cells is mainly mediated by LFA-1 (CD11a/CD18) and CD2 molecules. Low-affinity binding of resting T cells can be transiently upregulated by cross-linking of CD3-TCR (T cell receptor) complexes. This inside-out signaling influences i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1211
更新日期:1993-09-01 00:00:00
abstract::Patients treated with monoclonal antibodies and cytokines for cancer receive often co-medication, which may influence treatment efficacy. Therefore, we investigated with a flowcytometric cytotoxicity assay the effect of several immunomodulatory drugs on antibody dependent cellular cytotoxicity (ADCC), interleukin-2 (I...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.05.002
更新日期:2007-03-01 00:00:00
abstract::Activation of lck-fyn kinases during T cell receptor signaling leads to Vav phosphorylation, activation of downstream targets including Rac1, and a transient decline in ezrin and moesin phosphorylation. We have shown that age increases Rac1 activity and lowers ezrin and moesin phosphorylation in resting mouse CD4 cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.06.001
更新日期:2009-01-01 00:00:00
abstract::The aim of this study was to investigate the effect of fasting-induced orexin-A (OXA) on inflammation and macrophage phagocytic activity. Fifty six male wistar rats were fasted for 36 h to stimulate OXA synthesis. In 24 rats, air pouches were induced subcutaneously in the intrascapular area. After (6 h) carrageenan in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.005
更新日期:2011-01-01 00:00:00
abstract::A decapeptide (1182-1191) derived from the bovine interphotoreceptor retinoid-binding protein (IRBP) was found to contain two completely distinct antigenic sites when tested in Lewis rats. One site, localized in sequence 1182-1191, is the core of the immunodominant and highly uveitogenic determinant of IRBP. The secon...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90181-n
更新日期:1992-03-01 00:00:00
abstract::The delayed-type hypersensitivity (DTH) reaction, a peripheral expression of cell-mediated immunity is still a crucial in vivo immunological test. Nevertheless, the biological significance of its time course remains unclear. Thus, an exhaustive study of DTH was undertaken in mice immunized with increasing doses of she...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90287-y
更新日期:1992-07-01 00:00:00