Selective modulation of IFN-gamma mRNA stability by IL-12/NKSF.


:We investigated the role of IL-12 in regulating IL-2 and IFN-gamma production in primary culture of human T cells. Addition of neutralizing antiserum against the 40-kDa subunit of IL-12 to PHA-stimulated PBMC markedly reduced both IFN-gamma protein production and mRNA accumulation and stability. Moreover, concurrent treatment of partially purified T cells (> 90% CD3+) with PHA and rIL-12 selectively enhanced IFN-gamma mRNA stability and protein production, while IL-2 protein and mRNA levels were unaffected. These studies also show that IFN-gamma and IL-2 mRNA stability are temporally dissociated during the course of T cell activation, and we propose that this dissociation may be mediated through the production of IL-12. The effect of IL-12 on modulation of IFN-gamma mRNA turnover is not associated with detectable changes in either the levels or affinity of cytoplasmic RNA-binding proteins capable of recognizing AU-rich sequences in the 3'UTR of IFN-gamma mRNA.


Cell Immunol


Cellular immunology


Nagy E,Buhlmann JE,Henics T,Waugh M,Rigby WF




Has Abstract


1994-12-01 00:00:00














  • Immunosuppression and induction of anergy by CTLA4Ig in vitro: effects on cellular and antibody responses of lymphocytes from rats with experimental autoimmune myasthenia gravis.

    abstract::The pathogenic antibody response to acetylcholine receptor (AChR) in experimental autoimmune myasthenia gravis (EAMG) is T cell dependent. Therefore, it should be possible to design specific immunotherapeutic approaches to treat EAMG (and human MG) by interfering with AChR-specific helper T cells. Productive T cell ac...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: McIntosh KR,Linsley PS,Drachman DB

    更新日期:1995-11-01 00:00:00

  • Comparison of the capacity of murine and human class I MHC molecules to stimulate T cell activation.

    abstract::The mechanism underlying the apparent differences in the capacity of murine and human class I MHC molecules to function as signal transducing structures in T cells was examined. Cross-linking murine class I MHC molecules on splenic T cells did not stimulate an increase in intracellular calcium ([Ca2+]i) and failed to ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Gur H,Wacholtz MC,Lie WR,Lipsky PE,Geppert TD

    更新日期:1992-10-15 00:00:00

  • An increased proportion of circulating Th22 and Tc22 cells in psoriasis.

    abstract::Psoriasis is a common dermatosis mediated by T cells. This study investigated the correlation of Th22 cells and Tc22 cells with psoriasis. A total of 30 psoriasis patients and 11 age- and sex-matched healthy controls were recruited for this study. The proportions of circulating Th22 and Tc22 cells, expression of aryl ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Luan L,Ding Y,Han S,Zhang Z,Liu X

    更新日期:2014-08-01 00:00:00

  • Mesenchymal stromal cells use PGE2 to modulate activation and proliferation of lymphocyte subsets: Combined comparison of adipose tissue, Wharton's Jelly and bone marrow sources.

    abstract::Due to their immunomodulatory properties, adipose tissue (AT) and Wharton's Jelly (WJ) constitute valuable alternatives to BM as sources of MSCs for managing graft-versus-host disease. To ensure the efficiency of AT- and WJ-MSCs implies the characterization of their immunomodulatory functions in comparison to those of...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Najar M,Raicevic G,Boufker HI,Fayyad Kazan H,De Bruyn C,Meuleman N,Bron D,Toungouz M,Lagneaux L

    更新日期:2010-01-01 00:00:00

  • Both exogenous commensal and endogenous self antigens stimulate T cell proliferation under lymphopenic conditions.

    abstract::Within lymphopenic recipients, naïve T cells undergo proliferation that is induced by homeostatic mechanisms. Earlier studies have demonstrated that commensal antigens play a key role in inducing the proliferation. However, a relative contribution of endogenous self antigens in this process has not been formally inves...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Do JS,Foucras G,Kamada N,Schenk AF,Shaw M,Nuñez G,Paul WE,Min B

    更新日期:2012-01-01 00:00:00

  • Eomesodermin driven IL-10 production in effector CD8+ T cells promotes a memory phenotype.

    abstract::CD8+ T cell differentiation is controlled by the transcription factors T-bet and Eomesodermin, in concert with the cytokines IL-2, IL-10 and IL-12. Among these pathways, the mechanisms by which T-box proteins and IL-10 interact to promote a memory T cell fate remain poorly understood. Here, we show that Eomes and IL-1...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Reiser J,Sadashivaiah K,Furusawa A,Banerjee A,Singh N

    更新日期:2019-01-01 00:00:00

  • Migration of lymphocytes through endothelial cell monolayers: augmentation by interferon-gamma.

    abstract::During normal lymphocyte recirculation and in chronic inflammation, lymphocytes emigrate from blood into the perivascular tissue. The mechanism of lymphocyte migration through the endothelial cell (EC) layer of blood vessels is poorly understood. To identify factors that control lymphocyte emigration, a method has bee...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Oppenheimer-Marks N,Ziff M

    更新日期:1988-07-01 00:00:00

  • Augmentation of innate immunity by low-dose irradiation.

    abstract::The effect of low-dose irradiation on the immune system was investigated in mice. When a 0.2 Gy dose of X-ray irradiation was administered every other day for a total of four times, the number of lymphocytes yielded by the liver, spleen and thymus decreased at the initial stage (around day 10). At this stage, NK cells...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ren H,Shen J,Tomiyama-Miyaji C,Watanabe M,Kainuma E,Inoue M,Kuwano Y,Abo T

    更新日期:2006-11-01 00:00:00

  • Induction of unresponsiveness to Heymann's nephritis: inhibited by monoclonal antibody to CD4 but not to CD8.

    abstract::The effects of mAb therapy to CD4 or CD8 on induction of unresponsiveness to Heymann's nephritis by preimmunization with renal tubular antigen in IFA. Anti-CD4 mAbs (MRC Ox35) given for 2 weeks after RTA/IFA completely prevented the induction of resistance to HN, all rats developing proteinuria as well as high titers ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Quiza CG,Leenaerts PL,Hall BM

    更新日期:1991-04-01 00:00:00

  • FK-506 and cyclosporin A inhibit highly similar signal transduction pathways in human T lymphocytes.

    abstract::This report compares the ability of cyclosporin A and FK-506 to inhibit human T cell activation triggered via cell surface molecules that utilize different intracellular processes. We stimulated highly purified peripheral blood T lymphocytes with mitogens (Con A and PHA), ionomycin + PMA, or monoclonal antibodies spec...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Lin CS,Boltz RC,Siekierka JJ,Sigal NH

    更新日期:1991-04-01 00:00:00

  • B cell differentiation factor-induced B cell maturation: regulation via reduction in cAMP.

    abstract::We have previously described a novel human B cell differentiation factor (BCDF), 446-BCDF, that is distinct biochemically and functionally from other cytokines. Since signal transduction pathways involved in human B cell differentiation have been incompletely studied and are poorly understood, we assessed the effects ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Huang R,Cioffi J,Berg K,London R,Cidon M,Maayani S,Mayer L

    更新日期:1995-04-15 00:00:00

  • Intrathymic T cell repertoire after prenatal trinitrobenzene-sulfonic acid-treatment.

    abstract::It is still a matter of debate, whether tolerance toward self-non-MHC antigens is due to intrathymic deletion or to regulatory processes in the periphery. To further pursue this question, responsiveness toward TNP and an anti-TNP monoclonal antibody (Sp6) carrying a recurrent idiotype was evaluated in prenatally trini...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Zöller M

    更新日期:1990-03-01 00:00:00

  • Studies on the relationship of human natural killer and lymphokine-activated killer cells with lysosomal staining and analysis of surface marker phenotypes.

    abstract::We have previously demonstrated that natural killer (NK) cells are lysosome-rich and stain more intensely with lysosomotropic agents such as neutral red and quinacrine (Qu) than do non-NK cells. In this study we combined the quantitation of Qu staining with surface marker staining to define subpopulations of NK cells....

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Shau H,Gray D,Mitchell MS

    更新日期:1988-08-01 00:00:00

  • Experimental food allergy leads to adipose tissue inflammation, systemic metabolic alterations and weight loss in mice.

    abstract::To investigate the consequences of food allergy in adipose tissue and metabolism, we used a murine model in which mice have been sensitized subcutaneously with ovalbumin and further received antigen-containing diet. Allergic mice presented a significant weight loss 7 days after oral challenge with a concomitant decrea...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Dourado LP,Noviello Mde L,Alvarenga DM,Menezes Z,Perez DA,Batista NV,Menezes GB,Ferreira AV,de Souza Dda G,Cara DC

    更新日期:2011-01-01 00:00:00

  • Antigenic phenotype of TdT-positive cells in human peripheral blood.

    abstract::Double immunofluorescence studies for terminal deoxynucleotidyl transferase (TdT) and leucocyte surface membrane antigens have been used to characterize the small subpopulation of TdT-positive cells in human peripheral blood. The predominant antigens demonstrated were those coded for by the major histocompatibility co...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Bradstock KF,Kerr A,Bollum FJ

    更新日期:1985-02-01 00:00:00

  • In vitro and in vivo suppression of interleukin-2-activated killer cell activity by chimeric proteins between interleukin-2 and Pseudomonas exotoxin.

    abstract::The biological effects of IL-2 are mediated through high (complex of alpha and beta chain) or intermediate (beta chain) affinity IL-2 receptors. Previously, chimeric proteins composed of IL-2 and Pseudomonas exotoxin (IL-2-PE) were shown to be specifically cytotoxic to cells bearing IL-2 receptors. It has also been sh...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Puri RK,FitzGerald D,Leland P,Kozak RW,Pastan I

    更新日期:1992-09-01 00:00:00

  • Characterization of functional GTP binding proteins in Jurkat T cell mutants lacking either CD3-Ti or CD2 surface receptors.

    abstract::G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T lymphocytes. Given that mature T cells which lack antigen receptors (CDl-Ti) are refractory to stimulation through CD2 or other accessory molecules, T cell rece...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Moingeon P,Jin YJ,Stebbins CC,Lopez PA,Alcover A,Reinherz EL

    更新日期:1990-07-01 00:00:00

  • Specific immune responses but not basal functions of B and T cells are impaired in aged mice.

    abstract::Senescence is characterized by several alterations in the immune system. Such modifications can be found in lymphoid organs as well as in the cellular components of the immune system. Several reports have suggested that immune dysfunction can affect both T and B cells, but T cells have been shown to be more susceptibl...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Speziali E,Santiago AF,Fernandes RM,Vaz NM,Menezes JS,Faria AM

    更新日期:2009-01-01 00:00:00

  • Stimulation of murine B cells by the combination of calcium ionophore plus phorbol ester.

    abstract::The ability of calcium ionophore and phorbol ester to stimulate entry into S phase was assessed during short-term cultures of murine B cells extensively depleted of T cells. Neither ionomycin alone nor PMA alone stimulated DNA synthesis. However, the combination of ionomycin plus PMA acted in synergy to induce initiat...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Rothstein TL,Baeker TR,Miller RA,Kolber DL

    更新日期:1986-10-15 00:00:00

  • Influence of immunomodulatory drugs on the cytotoxicity induced by monoclonal antibody 17-1A and interleukin-2.

    abstract::Patients treated with monoclonal antibodies and cytokines for cancer receive often co-medication, which may influence treatment efficacy. Therefore, we investigated with a flowcytometric cytotoxicity assay the effect of several immunomodulatory drugs on antibody dependent cellular cytotoxicity (ADCC), interleukin-2 (I...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Flieger D,Varvenne M,Kleinschmidt R,Schmidt-Wolf IG

    更新日期:2007-03-01 00:00:00

  • Cellular immune response evaluation of cutaneous leishmaniasis patients cells stimulated with Leishmania (Viannia) braziliensis antigenic fractions before and after clinical cure.

    abstract::American cutaneous leishmaniasis (ACL) is a disease where susceptibility or resistance is dependent on T cell response. This is characterized by an increased in CD4⁺ T cells, capable of inducing opposite disease profiles, and CD8⁺ T cells, that are related to immuno protection. We characterized T lymphocytes from pati...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Brelaz-de-Castro MC,de Almeida AF,de Oliveira AP,de Assis-Souza M,da Rocha LF,Pereira VR

    更新日期:2012-10-01 00:00:00

  • Macrophage I-A/I-E expression and macrophage-stimulating lymphokines in murine lupus.

    abstract::Seeking common abnormalities in mice genetically predisposed to lupus-like autoimmune disease, we investigated (1) the ontogeny of Ia antigens (I-A/I-E) on the surfaces of resident peritoneal macrophages (rpM phi) of lupus and normal mice, (2) spontaneous and lectin-induced in vitro production of M phi-stimulating fac...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kofler R,Schreiber RD,Dixon FJ,Theofilopoulos AN

    更新日期:1984-08-01 00:00:00

  • Prolonged allogeneic and xenogeneic microchimerism in unmatched primates without immunosuppression by intrathymic implantation of CD34+ donor marrow cells.

    abstract::Engraftment of stem cell-enriched donor marrow implanted in the thymus of a foreign host might facilitate acceptance of donor-specific organ or tissue grafts. To test this hypothesis, allogeneic and xenogeneic CD34+ marrow cells from unrelated adult male baboons and humans were injected intrathymically in eight infant...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Allen MD,Weyhrich J,Gaur L,Akimoto H,Hall J,Dalesandro J,Sai S,Thomas R,Nelson KA,Andrews RG

    更新日期:1997-11-01 00:00:00

  • Comparative effectiveness of lymphotoxin anticarcinogenic and tumor cell growth-inhibitory activities.

    abstract::Prevention of ultraviolet radiation- or chemical carcinogen-induced morphologic transformation and inhibition of tumor-producing transformed cell growth by lymphotoxin and by normal spleen leukocytes were quantitatively compared to define the antineoplastic activity spectra of these natural immune mediators. When Syri...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Evans CH,Heinbaugh JA,DiPaolo JA

    更新日期:1983-03-01 00:00:00

  • The host response in graft versus host disease. II. The emergence of host protective cells is in part determined by background genomic compatibility.

    abstract::Murine graft versus host (GVH) disease takes two forms depending on the parental/F1 strain combination employed. In an accompanying paper (Singh et al., Clin. Immunol. 151, 1993) many of the clinical features of these two forms of GVH disease are described. In addition to these clinical characteristics, acute lethal G...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Mann RA,Singh AB,Singh M,Jetzt AE

    更新日期:1993-10-01 00:00:00

  • Antitumor synthetic lipid A analog DT-5461a upregulates cytokine expression in a murine macrophage cell line through LPS pathway.

    abstract::We previously showed that DT-5461a, a lipid A analog, has antitumor activity and relatively low toxicity in animals. To clarify its immunostimulatory effect we have studied the cytokine inducibility of DT-5461a and the mechanism involved in the cytokine expression in macrophages. While the activity is lower than that ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Akahane K,Someya K,Tsutomi Y,Akimoto T,Tohgo A

    更新日期:1994-04-15 00:00:00

  • Interferon-gamma receptor polymorphisms determine strain differences in accessibility of activated lymphocyte NK-triggering antigens to recognition by self-reactive NK cells.

    abstract::The "NK-triggering-antigen regulator" (Nktar) gene is a locus identified in the C57BL/6 genome which regulates the ability of unlabeled activated Con A blasts to compete for recognition of labeled syngeneic Con A blasts by BALB/c NK cells. Linkage analysis on Con A blasts from (BALB/c x CByB6F1) N2 backcross progeny f...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Chou SR,Brownell A,Ko MS,Kaplan J

    更新日期:2000-03-15 00:00:00

  • Estrogen induces suppression of natural killer cell cytotoxicity and augmentation of polyclonal B cell activation.

    abstract::Estrogen displays potent immunoregulatory properties. In the present study we investigated the in vivo effect of estrogen on natural killer (NK) cell cytotoxic activity in several mouse strains. After castration, 17 beta-estradiol was administered in low or high doses. After 3-6 weeks of hormone administration an in v...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Nilsson N,Carlsten H

    更新日期:1994-10-01 00:00:00

  • Human T-lymphocyte chemotactic activity: nature and production in response to antigen.

    abstract::Previous studies have shown that supernatants from concanavalin A-stimulated human peripheral blood mononuclear cells and isolated Leu-2 suppressor/cytotoxic T cells are chemotactic for Leu-3 helper/inducer T cells. The current study shows that lymphocyte chemotactic factor (LCF) is also produced following antigen (te...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Potter JW,Van Epps DE

    更新日期:1986-01-01 00:00:00

  • Cytotoxic properties of CD4(+) T-cell clones which lyse HLA class II negative autologous non-small-cell lung cancer cells.

    abstract::In this report, a subset of CD4(+) cells which kills autologous HLA class I positive and HLA class II negative non-small-cell lung cancer (NSCLC) cells is described. Killing was performed both by direct cytolytic mechanisms and by apoptosis. The peculiar characteristic of these effectors was their capability of lysing...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Semino C,Ferlazzo G,Ratto GB,Melioli G

    更新日期:1999-09-15 00:00:00