Abstract:
:Poxviruses are a large, complex group of highly successful pathogens that cause disease in humans and other animals. They encode several proteins postulated to be involved in the evasion of host immunity and therefore serve as excellent models for understanding virus-host interaction during the early stages of viral infection. Vaccinia virus, the best characterized member of the poxviridae family, encodes a 35-kDa major secretory polypeptide termed vaccinia virus complement control protein (VCP), which is structurally related to the family of human and mouse complement control proteins. Members of the family of complement control proteins have been shown to inhibit complement-mediated opsonization of bacteria and induction of inflammatory and phagocytic responses in vitro. Insertional inactivation of the VCP gene results in attenuation of viral virulence in vivo. The role of host complement in the inflammatory response to poxvirus infection has not been systematically investigated. Prior to determining the role of VCP on inflammatory responses in vivo, we decided to investigate the role of host complement in the progression of viral infection. We have compared the effects of injection of cowpox virus, primarily a rodent virus, into footpads of congenic mice strains B10.D2/nSnJ (C5-sufficient) and B10.D2/oSnJ (C5-deficient). The effects of the injection were monitored macroscopically by measuring the specific swelling response immediately following primary injection and subsequently after reinfection and by histological examination of the stained sections of the footpads. Our results indicate that there is a significant variation in the primary response in the two different mouse strains to cowpox virus infection. The specific swelling response observed in measurements from the footpads of the B10.D2/oSnJ mice was significantly greater, persisted for a longer duration, and was accompanied by severe ulceration, edema, induration, and hemorrhaging. Reinjection of the footpads after a 3-month period, during which time the swelling had subsided and the footpad had fully recovered to its original size and appearance, showed no significant differences between the two strains. This strongly suggests that the host complement plays a significant role during the initial response to poxvirus infection.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Miller CG,Justus DE,Jayaraman S,Kotwal GJdoi
10.1006/cimm.1995.1086subject
Has Abstractpub_date
1995-05-01 00:00:00pages
326-32issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(85)71086-6journal_volume
162pub_type
杂志文章abstract::Extracellular adenine nucleotides are inhibitors of the human natural killer cell line NK3.3 natural cytotoxicity activity. Natural cytotoxicity was inhibited approximately 26% by 1 mM ATP and 21% by 1 mM ADP. 5'-Adenylyl imidodiphosphate, a nonhydrolyzable ATP analog, inhibited natural cytotoxicity by 41% at a concen...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(95)80028-h
更新日期:1995-02-01 00:00:00
abstract::We tested the effect of different inhibitors of cytochrome P450 on tumor necrosis factor (TNF) production. Metyrapone and SKF525A (100 and 50 mg/kg, ip, respectively) suppressed serum TNF induced by cotreatment with endotoxin (LPS), (2.5 micrograms/mouse). Inhibition was independent of endogenous corticosteroids since...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1209
更新日期:1993-09-01 00:00:00
abstract::Murine graft versus host (GVH) disease takes two forms depending on the parental/F1 strain combination employed. In an accompanying paper (Singh et al., Clin. Immunol. 151, 1993) many of the clinical features of these two forms of GVH disease are described. In addition to these clinical characteristics, acute lethal G...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1220
更新日期:1993-10-01 00:00:00
abstract::The mechanism underlying the apparent differences in the capacity of murine and human class I MHC molecules to function as signal transducing structures in T cells was examined. Cross-linking murine class I MHC molecules on splenic T cells did not stimulate an increase in intracellular calcium ([Ca2+]i) and failed to ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90254-m
更新日期:1992-10-15 00:00:00
abstract::ErbB-2 is ubiquitinated and degraded when dissociated from its membrane chaperone or bound by specific antibody. Reagents which induce such degradation have demonstrated antitumor activity and may impact ErbB-2 immunogenicity. To further understand ErbB-2 degradation and immunogenicity, a glycine-alanine repeat (GAr) ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1853
更新日期:2001-09-15 00:00:00
abstract::Chemokine receptors CCR5 and CXCR4 are of major importance in the pathogenesis of HIV-1 infection because they are co-receptors for human immunodeficiency virus (HIV) entry. We examined the frequency of CD3-CD56+CCR5+ and CD3-CD56+CXCR4+ in HIV-infected long-term slow progressors (SPs), HIV typical progressors (TPs) w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.01.010
更新日期:2008-01-01 00:00:00
abstract::Although engagement of MHC class II molecules on human monocytes triggers various cellular events, their possible role in monocyte death is yet unknown. We demonstrate that ligation of MHC class II on primary monocytes induces a rapid cell death that has all the characteristics of monocyte apoptosis, does not require ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1428
更新日期:1999-02-25 00:00:00
abstract::PNK-E and G7 mAbs regulate porcine NK and ADCC activities by binding to distinct NK function-associated trigger molecules on porcine NK cells. Previous work demonstrates that PNK-E mAb binds to a 205-kDa tetrameric molecule composed of two 47-kDa peptides and two 50-kDa peptides and G7 mAb binds to a distinct 40-kDa h...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1026
更新日期:1993-02-01 00:00:00
abstract::Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a chronically intestinal autoimmune disease, the pathological mechanisms of which are not very clear. Wild type p-53 induced phosphatase 1 (Wip1), a serine/threonine protein phosphatase, has been reported to negatively reg...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.07.012
更新日期:2016-12-01 00:00:00
abstract::Treatment of human blood mononuclear cells with nanomolar concentrations of phorbol 12,13-dibutyrate (P(Bu)2) induced their aggregation. The phenomenon was seen within a few minutes and reached its maximum manifestation after 20 min. At this time, 20-30% of unfractionated and nylon wool-passed mononuclear leukocytes w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90313-1
更新日期:1983-01-01 00:00:00
abstract::Human B-cell lines derived from normal donors (LCL) or from Burkitt lymphomas (BL) were compared for their sensitivity to natural (NK) and interferon (IFN)-activated (IAK) cytotoxicity, mediated by effector cells from normal human blood. In four cases, a BL and an LCL line were derived from the same donor and had been...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90382-4
更新日期:1984-07-01 00:00:00
abstract::Adhesion of hematopoietic cells to extracellular matrix components is important for blood cell development. However, little is known regarding the potential influence of IL-3 on this process for precursor B cells and Flt3-ligand has not yet been implicated in induction of adhesion of any blood cell types to extracellu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1318
更新日期:1998-07-10 00:00:00
abstract::Although it is well known that the CD3/T-cell receptor (TCR) complex modulates from the surface of T cells upon exposure to monoclonal antibodies (mAb) directed against it, the fate of bound mAb has not been yet elucidated. We therefore perform direct binding experiments of 125I-labeled mAb against CD3 or TCR to inves...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90209-2
更新日期:1988-10-01 00:00:00
abstract::Spontaneous rosette formation in humans is restricted to a subpopulation of the circulating T cells. We have previously shown that the interaction between lymphocytes and autologous red blood cells (auto-RBC) is not mediated by a self-recognition mechanism, since allogeneic (allo-) RBC bind to T cells through the same...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90345-4
更新日期:1985-11-01 00:00:00
abstract::The phenotypic and functional relationships among the various B-cell subsets is of importance for better understanding studies of the immune system. In this report, the realm of B cells encompassed by Lyb 5, Ly 1, and xid has been examined through the use of the alloantiserum anti-Lyb 5, the unique functional properti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90077-2
更新日期:1985-04-15 00:00:00
abstract::Using analogues of arginine to inhibit nitric oxide (NO.) production, investigators have demonstrated the intermediary role of NO. in a variety of physiological events including the antimicrobial activity exhibited by macrophages in vitro. In an effort to establish the effector function of NO. in the antimicrobial act...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1048
更新日期:1994-02-01 00:00:00
abstract::The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we com...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90083-9
更新日期:1987-04-01 00:00:00
abstract::Exposure to pathogens induces dendritic cells to release inflammatory cytokines and chemokines. The inflammatory response is controlled by endogenous agents such as anti-inflammatory cytokines, glucocorticoids, anti-inflammatory neuropeptides, and lipid mediators. This study is the first report on the inhibition by pr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00158-8
更新日期:2003-06-01 00:00:00
abstract::The cell-averaged microviscosity of intact murine peritoneal mononuclear phagocytes in various stages of activation was assessed by quantifying fluorescent depolarization of 1,6-diphenyl-1,3,5-hexatriene. Macrophages activated in vivo with Mycobacterium bovis, strain BCG, were significantly more fluid than resident pe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90026-8
更新日期:1987-02-01 00:00:00
abstract::Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus transformed B cells is mainly mediated by LFA-1 (CD11a/CD18) and CD2 molecules. Low-affinity binding of resting T cells can be transiently upregulated by cross-linking of CD3-TCR (T cell receptor) complexes. This inside-out signaling influences i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1211
更新日期:1993-09-01 00:00:00
abstract::We investigated the role of HLA-DR molecules in T cell stimulation by staphylococcal enterotoxin A (SEA). Previous results with immobilized purified HLA-DR preincubated with peptide showed that peptide-specific T cell clones were able to bind to and proliferate in response to purified HLA-DR/peptide complexes in the a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1255
更新日期:1993-10-15 00:00:00
abstract::The ontogeny of T cells which express major histocompatibility complex (MHC)-unrestricted T cell antigen-binding molecules (TABM) on the cell membrane was investigated. We used a rabbit anti-mouse TABM antiserum to investigate the expression of TABM by subsets of adult thymocytes, peripheral T cells, and thymocytes du...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90171-m
更新日期:1990-10-01 00:00:00
abstract::This report describes a model for investigating the role of the thymus in regulating natural killer (NK) cell activity in vivo. Evidence is presented that the thymus can regulate NK cells, and that at least some NK cells can develop without thymic help. Marrow from thymectomized rats depleted of circulating T cells by...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90201-1
更新日期:1985-02-01 00:00:00
abstract::Strong delayed-type hypersensitivity (DTH) to Babesia microti was elicited when intraerythrocytic parasites (IEP) were inoculated subcutaneously into the flank of normal mice 6 to 14 days before challenge in the ipsilateral footpad with 10(8) IEP. Intraperitoneal or intravenous administration of antigen did not sensit...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90289-3
更新日期:1986-04-01 00:00:00
abstract::The kinetic and the H-2 requirements for the induction of nucleoside-specific suppression were examined in several strains of mice; specifically, whether adenosine (A)-coupled spleen cells given intravenously suppress the primary response to adenosine-KLH. The adenosine system was chosen because C57Bl/6 mice were orig...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90333-7
更新日期:1983-02-01 00:00:00
abstract::Several lines of evidence suggest that nitric oxide (NO), generated through nitric oxide synthase (NOS) by cleavage of terminal guanidino nitrogen from L-arginine, mediates tumor cell killing by mononuclear phagocytes. Natural killer (NK) cells are cytotoxic effector cells that lyse a variety of tumor and virus-infect...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1215
更新日期:1994-08-01 00:00:00
abstract::We tested the impact of interleukin-12 (IL-12) and of cytotoxic leukocytes (particularly natural killer (NK) cells) from normal and HIV-1-infected subjects on lysis of human monocytes infected with Mycobacterium tuberculosis. Nylon wool nonadherent cells stimulated with interleukin-2 (IL-2) or with IL-12 developed sig...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1196
更新日期:1994-07-01 00:00:00
abstract::B-1a cells represent a distinct B cell population with unique phenotype, self-renewing capacity and restricted Igμ repertoire. They primarily locate in body cavity and also exist in spleen. The different subpopulations of B-1a cells are heavily affected by local environment. Our previous studies revealed that MARVEL-d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104100
更新日期:2020-06-01 00:00:00
abstract::The loss of thymic function with age may be due to diminished numbers of T-cell progenitors and the loss of critical mediators within the thymic microenvironment. To assess the molecular changes associated with this loss, we examined transcriptomes of progressively aging mouse thymi, of different sexes and on caloric-...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.03.008
更新日期:2007-01-01 00:00:00
abstract::Antigen-nonspecific CD8+ T suppressor cells, which suppressed delayed-type hypersensitivity (DTH) against sheep red blood cells in BALB/c mice, were induced by incubating spleen cells from mice treated with 7,12-dimethylbenz[a]anthracene (DMBA), a tumor initiator, with 12-O-tetradecanoylphorbol 13-acetate (TPA), a tum...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90167-a
更新日期:1991-12-01 00:00:00