Abstract:
:The lineage of lymphokine-activated killer (LAK) cells is poorly understood. To examine the relationship between LAK and natural killer (NK) cells we utilized two congenitally immunodeficient mice, namely severe combined immunodeficient (scid) and athymic (nude) mice that lack T cells but have normal NK cells. LAK activity was evaluated by the ability to lyze NK-resistant P815 cells. When cultured with human recombinant interleukin 2, splenocytes of scid and nude mice could generate LAK activity at levels comparable to or more than those of normal C.B-17 mice. LAK effector cells in these immunodeficient mice as well as normal mice had the phenotype resembling that of NK cells with asialo-GM1 (aGM1) expression. In vivo treatment with anti-aGM1 antiserum completely abolished the induction of LAK activity from splenocytes of normal mice. In contrast, LAK activity in splenocytes of scid and nude mice was still demonstrable even after this treatment, indicating that most LAK precursors in both mice were cells without aGM1 antigen. The aGM1- progenitors for LAK activity, probably in common with NK progenitors, appeared to be more expanded in scid and nude mice than in normal mice. The use of such congenitally immunodeficient mice should be helpful in studying the differentiation step of LAK as well as NK cells from their precursors.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Hasui M,Saikawa Y,Miura M,Takano N,Ueno Y,Yachie A,Miyawaki T,Taniguchi Ndoi
10.1016/0008-8749(89)90190-1subject
Has Abstractpub_date
1989-04-15 00:00:00pages
230-9issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(89)90190-1journal_volume
120pub_type
杂志文章abstract::Two monoclonal antibodies specific for different rat T-cell subpopulations, the anti-helper-T-cell antibody, W3/25, and the OX8 suppressor cell antibody were used to investigate lectin-stimulated T-lymphocyte differentiation of F-344 rat bone marrow cells in culture. Cytofluorometric analysis of freshly isolated lymph...
journal_title:Cellular immunology
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更新日期:1983-12-01 00:00:00
abstract::The MCA 102 sarcoma has been defined by a variety of immunologic studies as a tumor lacking intrinsic immunogenicity. Nevertheless, we have recently demonstrated the feasibility of generating therapeutically effective lymphocytes for adoptive immunotherapy of this tumor. Procedures to achieve this required in vivo pri...
journal_title:Cellular immunology
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abstract::This report describes a model for investigating the role of the thymus in regulating natural killer (NK) cell activity in vivo. Evidence is presented that the thymus can regulate NK cells, and that at least some NK cells can develop without thymic help. Marrow from thymectomized rats depleted of circulating T cells by...
journal_title:Cellular immunology
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abstract::The susceptibility of murine embryonic cells to CTL lysis has been assessed. Zona-free blastocysts were not lysed during 6 hr incubation with CTL sensitized against the embryonic genotype and remained capable of implantation and normal development in vivo as well as outgrowth in vitro. Freshly dissociated cells from p...
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abstract::Interleukin-1 is a potent stimulator of arachidonic acid (AA) metabolism and this activity could be attributed to the activation of the prostaglandin-forming enzyme cyclooxygenase or of the arachidonic-releasing enzyme phospholipase A2 or both. Prostaglandin E2 (PGE2), a cyclooxygenase product, and LTB4 (5-(S),12-(R)-...
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
abstract::Short-term culture of murine lymphocytes in interleukin 2 (IL-2), in the absence of any priming antigen, has been shown to result in the differentiation of an activated killer cell population capable of potent cytotoxic activity against tumor cells. The progenitor and lineage of these lymphokine activated killer cells...
journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00
abstract::Estrogen displays potent immunoregulatory properties. In the present study we investigated the in vivo effect of estrogen on natural killer (NK) cell cytotoxic activity in several mouse strains. After castration, 17 beta-estradiol was administered in low or high doses. After 3-6 weeks of hormone administration an in v...
journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1984-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1677
更新日期:2000-08-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90238-m
更新日期:1990-11-01 00:00:00
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journal_title:Cellular immunology
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更新日期:2004-09-01 00:00:00
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journal_title:Cellular immunology
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更新日期:1998-07-10 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1002
更新日期:1995-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1998-03-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90007-5
更新日期:1989-06-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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更新日期:2009-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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更新日期:2018-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1983-09-01 00:00:00
abstract::We have previously shown that interleukin-2 (IL-2) is able to induce the generation of natural killer (NK) activity in bone marrow (BM) cells from mice pretreated with 5-fluorouracil. IL-2 alone could dose-dependently induce NK activity in marrow cells and interleukin-4 (IL-4) has dual effect on the NK activity in tha...
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更新日期:1991-08-01 00:00:00
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abstract::To investigate the process of tolerance induction we have developed an in vivo model using TCR beta-chain transgenic mice tolerized with the superantigen staphylococcal enterotoxin B. We have previously demonstrated that tolerized peripheral T cells were anergic when stimulated in vitro with immunogenic peptides, supe...
journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
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