Abstract:
:PHA stimulation of both freshly isolated and IL-2-dependent cultured T cells induced a rapid rise in intracytoplasmic calcium concentration. Chelation of extracellular calcium with EGTA resulted in a failure of PHA to induce a rise in intracytoplasmic calcium, resulting in the fresh T cells in an inhibition of IL-2 production, IL-2 receptor expression, and proliferation. However, cultured T cells grown in recombinant IL-2 were able to re-express IL-2 receptors and proliferate in response to PHA stimulation in the presence of EGTA. Thus the PHA-induced signal for expression of IL-2 receptors and T-cell proliferation differs in fresh and cultured T cells and required extracellular calcium in fresh but not in cultured T cells.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Watman NP,Crespo L,Davis B,Poiesz BJ,Zamkoff KWdoi
10.1016/0008-8749(88)90060-3subject
Has Abstractpub_date
1988-01-01 00:00:00pages
158-66issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(88)90060-3journal_volume
111pub_type
杂志文章abstract::Positive selection of T lymphocytes expressing self. MHC-restricted T cell receptors is mediated by MHC molecules expressed on thymic stroma. Among the more controversial aspects of this process is the relative role of MHC molecules on epithelial versus bone marrow-derived stromal elements (BM-APC). For CD4+ T cells, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0175
更新日期:1996-07-10 00:00:00
abstract::Cultures of gradient-purified human peripheral blood mononuclear cells (PBMC) have been employed to examine the effects of three bacteria-derived human leukocyte interferon subtypes on certain aspects of in vitro immune responses. The addition of highly purified IFN-alpha 1, -alpha 2, -alpha 2/alpha 1 to PMBC cultures...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90161-2
更新日期:1983-12-01 00:00:00
abstract::During ultrastructural studies of the early kinetics of anaphylactic degranulation and early and late recovery intervals from these release reactions induced in isolated, partially purified, cultured human lung mast cells stimulated to release histamine and granules by exposure to anti-IgE, we noted the ability of mas...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90068-2
更新日期:1987-03-01 00:00:00
abstract::The induction of ornithine decarboxylase (ODC) activity in lymphocytes is associated with activation and the initiation of cellular proliferation. ODC is also an essential component in tumor promotion. Phorbol myristic acetate (PMA) is a mitogen for lymphocytes, but can also promote tumor formation. Tumor promotion is...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90015-9
更新日期:1988-04-15 00:00:00
abstract::The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we com...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90083-9
更新日期:1987-04-01 00:00:00
abstract::Toll-like receptor (TLR) 2/1 signalling is linked to autophagy through transcriptional actions of the 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)-vitamin D receptor (VDR) complex. Population-specific effects have been reported for TLR2/1-VDR signalling. We hypothesized that population effects extend to autophagy and are in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104082
更新日期:2020-06-01 00:00:00
abstract::Imiquimod, a toll-like receptor 7 (TLR7) agonist, is an active pharmaceutical ingredient (API) established for the topical treatment of several dermal cancerous and precancerous skin lesions. Within this work, the immunostimulatory effect of imiquimod is further exploited in a transcutaneous immunization (TCI) approac...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.06.001
更新日期:2016-10-01 00:00:00
abstract::Interleukin (IL)-35 strongly suppresses the immune effects of CD8+ T cells. However, the mechanisms mediating these effects are not clear. Here, we investigated the potential inhibitory mechanisms of IL-35 using proteomics technology. The changes of differentially expressed proteins (DEPs) were evaluated using gene on...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.104022
更新日期:2020-02-01 00:00:00
abstract::Osteopontin (OPN) expression in tumors is associated with more aggressive tumor growth; however, several studies have suggested that OPN as a host protein can regulate tumor growth as well. OPN is produced by macrophages and T cells, and reportedly modifies macrophage function. Here, we have investigated the effect of...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2004.01.001
更新日期:2004-01-01 00:00:00
abstract::The cell-averaged microviscosity of intact murine peritoneal mononuclear phagocytes in various stages of activation was assessed by quantifying fluorescent depolarization of 1,6-diphenyl-1,3,5-hexatriene. Macrophages activated in vivo with Mycobacterium bovis, strain BCG, were significantly more fluid than resident pe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90026-8
更新日期:1987-02-01 00:00:00
abstract::Type 2 diabetes is a chronic disease with growing public health concern globally. Finding remedies to assist this health issue requires recruiting appropriate animal model for experimental studies. This study was designated to evaluate metabolic and immunologic changes in streptozotocin-nicotinamide induced diabetic r...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2014.04.004
更新日期:2014-05-01 00:00:00
abstract::Respiratory immunization is an attractive way to generate systemic and mucosal protective memory responses that are required for preventing mucosally transmitted infections. However, the molecular and cellular mechanisms for controlling memory T cell responses remain incompletely understood. In this study, we investig...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.07.006
更新日期:2016-12-01 00:00:00
abstract::The mechanism of polyclonal B cell activation in autoimmune diseases was investigated by using an autoreactive B cell clone established by somatic hybridization with B cells derived from NZB X NZW (B/W) F1 mice. Briefly, splenic B cells from B/W F1 mice were fused with M12.4.1, a mutant of a B cell line, in the presen...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90080-9
更新日期:1992-02-01 00:00:00
abstract::Theiler's murine encephalomyelitis virus-induced demyelinating disease (TMEV-IDD), a model for multiple sclerosis, is a chronic T cell-mediated disease. Development of clinical symptoms in susceptible mouse strains generally correlates with TMEV-specific delayed-type hypersensitivity (DTH) responses. These responses, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1303
更新日期:1993-12-01 00:00:00
abstract::We analyzed the effect of adherent cells on the induction of lymphokine-activated killer (LAK) cell activity by depleting adherent cells from peripheral blood lymphocytes (PBL) or by adding adherent cells to PBL before culture with interleukin-2. We found that adherent cells clearly down-regulate LAK cell induction. T...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90129-8
更新日期:1987-12-01 00:00:00
abstract::The aim of this study was to analyze in vivo the L3T4+ T-cell-subset-independent reactivity of Lyt2+ T cells toward transplantation alloantigens. To this end, we depleted normal mice of L3T4+ T cells by injection of monoclonal antibodies to the L3T4 antigen. This procedure not only led phenotypically to a disappearanc...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90059-7
更新日期:1988-01-01 00:00:00
abstract::During normal lymphocyte recirculation and in chronic inflammation, lymphocytes emigrate from blood into the perivascular tissue. The mechanism of lymphocyte migration through the endothelial cell (EC) layer of blood vessels is poorly understood. To identify factors that control lymphocyte emigration, a method has bee...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90324-3
更新日期:1988-07-01 00:00:00
abstract::Allergic asthma affects more women than men. It is mediated partially by IL-4/IL-13-driven polarization of monocyte-derived macrophages in the lung. We tested whether sex differences in asthma are due to differential IL-4 responsiveness and/or chemokine receptor expression in monocytes and monocyte-derived macrophages...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104252
更新日期:2021-02-01 00:00:00
abstract::CD48, the murine homolog of human CD58, binds to CD2 in rats and mice. Whereas inhibition of CD2 signaling leads to profound immunosuppression, no information is available on CD48-targeted therapy in the rat. We could show that anti-CD2 treatment (OX34) efficiently inhibited TCR-driven as well as CD2-mediated prolifer...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1204
更新日期:1997-11-25 00:00:00
abstract::The candidacidal activity (CA) of peritoneal cells (PC) in vitro was used as a measure of nonspecific microbicidal activity of phagocytes after intraperitoneal injection of mice with different adjuvants. Dilutions of PC were incubated with constant numbers of C. parapsilosis in a 96-well culture plate. The PC number c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90164-9
更新日期:1985-01-01 00:00:00
abstract::Vitamin D regulates homeostasis, anti-microbial response, and inflammation. The vitamin D receptors are expressed in the macrophages and other immune cells, regulating the transcription of many different genes, including those coding the anti-microbial peptides. One of the most severe complications of the SARS-CoV-2 i...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2020.104259
更新日期:2021-02-01 00:00:00
abstract::Unconventional T cells are gaining center stage as important effector and regulatory cells that orchestrate innate and adaptive immune responses. Human Vγ9/Vδ2 T cells are amongst the best understood unconventional T cells, as they are easily accessible in peripheral blood, can readily be expanded and manipulated in v...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2015.01.008
更新日期:2015-07-01 00:00:00
abstract::We describe here a culture system for studying the development, in vitro, of antigen-specific CD4 T cells from unprimed mice. T cells from young mice are initially exposed to antigen, such as pigeon cytochrome C or keyhole limpet hemocyanin, in the presence of adherent accessory cells and then allowed to proliferate i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1134
更新日期:1997-06-15 00:00:00
abstract::Idiotypic immunoglobulin, which can be considered to bear tumour-associated antigens in the context of B-cell lymphoma, has been obtained from the splenic A31 tumour, purified, and used to immunise syngeneic mice. On subsequent exposure to a lethal challenge of lymphoma cells, the mice showed no overt tumour developme...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90007-x
更新日期:1991-01-01 00:00:00
abstract::Interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) are major proinflammatory cytokines inducing the synthesis and release of many inflammatory mediators. They are involved in immune regulation, autoimmune diseases, and inflammation. Acanthoic acid, (-)-pimara-9(11),15-dien-19-oic acid, is a pimaradiene d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0154
更新日期:1996-06-15 00:00:00
abstract::Like the thymus, the bursa of Fabricius is a site of massive lymphopoiesis accompanied by cell death in vivo. In the present study we have, therefore, examined whether chicken bursa and thymus cells exhibit apoptosis. Bursa and thymus cells from SC chickens, 4-10 weeks of age, were incubated for 8-24 hr with various r...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1058
更新日期:1993-03-01 00:00:00
abstract::The effects of progesterone on murine suppressor cell function generated in allogeneic MLCs were investigated. BALB/c splenic lymphocytes stimulated in vitro with C3H/He cells significantly suppressed the proliferative response of BALB/c lymphocytes in a secondary MLC. This suppression was highly specific for the sens...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90377-5
更新日期:1983-03-01 00:00:00
abstract::Adenomatous polyposis coli (APC) functions are involved in the heterotypic interactions occurring between intestinal epithelial cells (IECs) and intra-epithelial lymphocytes (IELs). These interactions may be of interest in cancer prevention, since recent data provide evidence for lymphocyte mediated immunosurveillance...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00149-7
更新日期:2003-05-01 00:00:00
abstract::The purpose of this study is to assess the potential of dendritic cells transfected with PD-L1 recombinant adenovirus induces CD8+ T cell suppression and kidney allograft tolerance. To prove it, DCs transfected with PD-L1 recombinant adenovirus (DC/Ad-PD-L1) were transferred into the MHC-mismatched rat kidney transpla...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.007
更新日期:2011-01-01 00:00:00
abstract::The fine specificity of anti-Glu60Ala30Tyr10 (GAT) and anti-Glu60Ala40 (GA) proliferating cells was studied. T cells primed with GAT proliferate both to GAT and GA and GA-primed T cells proliferate also to GA and GAT. This cross-reactivity was unexpected given the results previously reported on the fine specificity of...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90309-x
更新日期:1983-01-01 00:00:00