当前位置： SCI文献检索 > CELLULAR IMMUNOLOGY期刊下所有文献 > Solid nanoemulsion as antigen and immunopotentiator carrier for transcutaneous immunization.

Solid nanoemulsion as antigen and immunopotentiator carrier for transcutaneous immunization.

Abstract：

:Imiquimod, a toll-like receptor 7 (TLR7) agonist, is an active pharmaceutical ingredient (API) established for the topical treatment of several dermal cancerous and precancerous skin lesions. Within this work, the immunostimulatory effect of imiquimod is further exploited in a transcutaneous immunization (TCI) approach based on a solid nanoemulsion (SN) formulation. SN contains a combination of imiquimod with the model peptide antigen SIINFEKL as a novel approach to omit needle and syringe and optimize dermal antigen administration. Excipients including sucrose fatty acid esters and the pharmaceutically acceptable oils MCT (middle chain triglycerides), avocado oil, jojoba wax and squalene are high pressure homogenized together with the antigen SIINFEKL. Freeze drying was performed to eliminate water and to achieve spreadable properties of the formulation for dermal administration. The influence of the different oil components was assessed regarding in vitro drug permeation in a Franz diffusion cell model using a murine skin setup. In vivo performance in terms of cytotoxic T-cell response was assessed in a C57BL/6 mouse model. Whereas Aldara® cream contains imiquimod in a dissolved state, the SN formulations carry the active in a suspended state. This resulted in a reduction of imiquimod permeation across murine skin from the SN when compared to Aldara® cream. In spite of this permeation rate reduction, each SN induced an in vivo immune response by specific T-cell lysis. A stabilized solid nanosuspension containing squalene/tocopherol exhibited a significantly higher performance (p⩽0.05) in comparison with Aldara® cream. MCT based SN exerted an in vivo effect comparable to Aldara®. In conclusion, anhydrous highly dispersed vehicles containing imiquimod in a submicron particle size distribution can represent promising formulations for TCI. The choice of the oil component has a strong influence on SN performance, independent of in vitro drug permeation.

journal_name

Cell Immunol

journal_title

Cellular immunology

authors

Gogoll K,Stein P,Lee KD,Arnold P,Peters T,Schild H,Radsak M,Langguth P

doi

10.1016/j.cellimm.2016.06.001

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

35-43

eissn

0008-8749

issn

1090-2163

pii

S0008-8749(16)30046-6

journal_volume

308

pub_type

杂志文章

相关文献

CELLULAR IMMUNOLOGY文献大全
  • Active target cell processes, possibly involving receptor-mediated endocytosis, are critical for expression of cytotoxicity by natural killer cell-derived cytolytic factor.

    abstract：:Inhibitors of energy metabolism, 2-deoxyglucose and cyanide were shown to inhibit NKCF-mediated lysis of L929 target cells at the same molar concentrations that effectively inhibited cellular ATP levels and the toxic effects of pseudomonas toxin A. In addition, inhibitors of receptor-mediated endocytosis, cytochalasin...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(86)90337-0

    authors： Deem RL,Niederlehner A,Targan SR

    更新日期：1986-10-01 00:00:00

  • Mesenchymal stromal cells use PGE2 to modulate activation and proliferation of lymphocyte subsets: Combined comparison of adipose tissue, Wharton's Jelly and bone marrow sources.

    abstract：:Due to their immunomodulatory properties, adipose tissue (AT) and Wharton's Jelly (WJ) constitute valuable alternatives to BM as sources of MSCs for managing graft-versus-host disease. To ensure the efficiency of AT- and WJ-MSCs implies the characterization of their immunomodulatory functions in comparison to those of...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2010.06.006

    authors： Najar M,Raicevic G,Boufker HI,Fayyad Kazan H,De Bruyn C,Meuleman N,Bron D,Toungouz M,Lagneaux L

    更新日期：2010-01-01 00:00:00

  • 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans.

    abstract：:Murine 4-1BB is an approximately 30-kDa glycoprotein expressed on activated T cells and plays a role in T-cell-mediated proliferative response. To date the majority of work on 4-1BB has been conducted in the mouse. To assess the role of 4-1BB in humans, mAbs were made against the recombinant human (rh) 4-1BB protein. ...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1996.0095

    authors： Garni-Wagner BA,Lee ZH,Kim YJ,Wilde C,Kang CY,Kwon BS

    更新日期：1996-04-10 00:00:00

  • IL-4 acts synergistically on the IL-2 response of an autoreactive T-cell clone; synergism correlates with increased intracellular IL-2, but not with a modified IL-2 receptor expression.

    abstract：:TE44, an H-2b-restricted, self-reactive T-cell line, did not produce autocrine-acting growth factors, neither after antigenic nor after mitogenic activation; they remained for their proliferation completely dependent on exogenously added IL-2. Administration of IL-4, which poorly promotes growth by itself, resulted in...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(90)90163-l

    authors： Vandenabeele P,Grooten J,Fiers W

    更新日期：1990-10-01 00:00:00

  • Rabbit bone marrow suppressor cells block the production or release of a soluble bone marrow growth factor.

    abstract：:Fc gamma-receptor (Fc gamma R)-bearing cells from normal rabbit bone marrow suppressed the constitutive proliferation rates of the remaining, Fc gamma R-, cells. In the absence of the suppressive influences of Fc gamma R+ cells, cells in the Fc gamma R- population spontaneously elaborated a soluble growth factor (GF) ...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(85)90012-7

    authors： Soderberg LS

    更新日期：1985-05-01 00:00:00

  • Human Vγ9/Vδ2 T cells: Innate adaptors of the immune system.

    abstract：:Unconventional T cells are gaining center stage as important effector and regulatory cells that orchestrate innate and adaptive immune responses. Human Vγ9/Vδ2 T cells are amongst the best understood unconventional T cells, as they are easily accessible in peripheral blood, can readily be expanded and manipulated in v...

    journal_title：Cellular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellimm.2015.01.008

    authors： Tyler CJ,Doherty DG,Moser B,Eberl M

    更新日期：2015-07-01 00:00:00

  • Ontogeny and expression of non-MHC-restricted T cell antigen-binding molecules by thymocytes and peripheral T cell subsets.

    abstract：:The ontogeny of T cells which express major histocompatibility complex (MHC)-unrestricted T cell antigen-binding molecules (TABM) on the cell membrane was investigated. We used a rabbit anti-mouse TABM antiserum to investigate the expression of TABM by subsets of adult thymocytes, peripheral T cells, and thymocytes du...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(90)90171-m

    authors： Rellahan BL,Cone RE

    更新日期：1990-10-01 00:00:00

  • The autoimmune mouse MRL/Mp-lpr/lpr contains cells with spontaneous cytotoxic activity against target cells bearing self-determinants.

    abstract：:Spontaneously cytotoxic cells possessing specificity and having a complex pattern of reactivity directed at least partly against self-determinants develop by the age of 10 weeks in the autoimmune mouse strain MRL/Mp-lpr/lpr (MRL-lpr) and by the age of 6 months in C57BL/6-lprl/lpr. Similar effector cells do not develop...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(88)90003-2

    authors： Muraoka S,Miller RG

    更新日期：1988-04-15 00:00:00

  • Evidence for protein tyrosine kinase involvement in CD6-induced T cell proliferation.

    abstract：:Several studies have demonstrated that addition of soluble anti-CD6 mAbs to 12-O-tetradecanoylphorbol 13-acetate (TPA)-treated naive T cells can induce cell proliferation. We showed in the present study that cell proliferation in TPA-treated T cell cultures can be enhanced several fold when the anti-CD6 mAbs are eithe...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1995.0006

    authors： Osorio LM,Ordonez C,Garcia CA,Jondal M,Chow SC

    更新日期：1995-11-01 00:00:00

  • Clonotypic heterogeneity of Lewis rat T cells specific for the encephalitogenic 68-86 region of myelin basic protein.

    abstract：:Experimental autoimmune encephalomyelitis was induced in a Lewis rat by sensitization with synthetic peptide GP68-86, representing the 68-86 sequence of guinea pig myelin basic protein (GPMBP). To delineate T cell determinants of GP68-86, lymph node cells from this rat were activated in culture with GP68-86 and were f...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(89)90099-3

    authors： Mannie MD,Paterson PY,U'Prichard DC,Thomas DW

    更新日期：1989-09-01 00:00:00

  • An autoreactive T hybridoma expressing nonspecific helper activity.

    abstract：:Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d present...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90371-x

    authors： Folks TM,Sell KW

    更新日期：1984-06-01 00:00:00

  • Beta2-integrin, LFA-1, and TCR/CD3 synergistically induce tyrosine phosphorylation of focal adhesion kinase (pp125(FAK)) in PHA-activated T cells.

    abstract：:Complete T cell activation requires not only a first signal via TCR/CD3 engagement but also a costimulatory signal through accessory receptors such as CD2, CD28, or integrins. Focal adhesion kinase, pp125(FAK) (FAK), was previously shown to be localized in focal adhesions in fibroblasts and to be involved in integrin-...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1999.1472

    authors： Tabassam FH,Umehara H,Huang JY,Gouda S,Kono T,Okazaki T,van Seventer JM,Domae N

    更新日期：1999-05-01 00:00:00

  • Studies on the mechanism of suppression of experimental allergic encephalomyelitis induced by myelin basic protein-cell conjugates.

    abstract：:The mechanism of suppression of experimental allergic encephalomyelitis (EAE) induced in Lewis rats by pretreatment with myelin basic protein (MBP) coupled to syngeneic spleen leukocytes (SL) was examined. Studies on the kinetics of the tolerance induction showed that pretreatment with MBP-SL suppressed EAE if given 7...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90159-x

    authors： McKenna RM,Carter BG,Sehon AH

    更新日期：1984-10-15 00:00:00

  • Inhibition of proinflammatory macrophage responses and lymphocyte proliferation in vitro by ethyl acetate leaf extract from Daphne gnidium.

    abstract：:Medicinal plants are considered immunomodulatory as they display various biological activities. There is no report addressing the anti-inflammatory effects of Daphne gnidium. In this study, we investigated the effects of D. gnidium ethyl acetate (EA) leaf extract on mice immune cell function in vitro. Production of pr...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2010.12.002

    authors： Harizi H,Chaabane F,Ghedira K,Chekir-Ghedira L

    更新日期：2011-01-01 00:00:00

  • The appearance of eosinophil-directed chemotactic inhibitory factor in the serum of complete Freund's adjuvant-treated guinea pigs.

    abstract：:When guinea pigs were treated by complete Freund's adjuvant (CFA), eosinophil-directed chemotactic inhibitory factor (ECIF) appeared in the serum of the treated animals. The inhibition of the response of eosinophils by ECIF was selective for the T cell-derived chemotactic factor, which has been isolated from DNP-ascar...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90202-8

    authors： Hirashima M,Tashiro K,Hayashi H

    更新日期：1984-11-01 00:00:00

  • Interleukin-12 is expressed by infiltrating macrophages and synovial lining cells in rheumatoid arthritis and osteoarthritis.

    abstract：:TH1 cytokines have recently been detected in rheumatoid arthritis (RA) and osteoarthritis (OA). For this reason we studied the TH-1-promoting cytokine IL-12 in synovial membranes from patients with RA and OA. IL-12 transcripts and protein were analyzed by reverse transcriptase-polymerase chain reaction (PCR) and immun...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1998.1363

    authors： Sakkas LI,Johanson NA,Scanzello CR,Platsoucas CD

    更新日期：1998-09-15 00:00:00

  • The regulatory role of Hyper-IL-6 in the differentiation of myeloid and erythroid progenitors derived from human cord blood.

    abstract：:This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34(+)CD38(-...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2006.07.008

    authors： Yu Z,Liu W,Liu D,Fan L

    更新日期：2006-05-01 00:00:00

  • The effects of an induced adenosine deaminase deficiency on T-cell differentiation in the rat.

    abstract：:Inherited deficiency of the enzyme adenosine deaminase (ADA) has been found in a significant proportion of patients with severe combined immunodeficiency disease and inherited defect generally characterized by a deficiency of both B and T cells. Two questions are central to understanding the pathophysiology of this di...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(85)90317-x

    authors： Barton RW

    更新日期：1985-10-15 00:00:00

  • Intranasal delivery of cationic liposome-protamine complex mRNA vaccine elicits effective anti-tumor immunity.

    abstract：:Immunization with synthetic mRNA encoding tumor-associated antigens is an emerging vaccine strategy for the treatment of cancer. In order to prevent mRNA degradation, promote antigen-presenting cells antigen presentation, and induce an anti-tumor immune response, we investigated the nasal administration of mRNA vaccin...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2020.104143

    authors： Mai Y,Guo J,Zhao Y,Ma S,Hou Y,Yang J

    更新日期：2020-08-01 00:00:00

  • Role of heme oxygenase-1 in potentiation of phagocytic activity of macrophages by taurine chloramine: Implications for the resolution of zymosan A-induced murine peritonitis.

    abstract：:Phagocytosis of pathogens by macrophages is crucial for the successful resolution of inflammation induced by microbial infection. Taurine chloramine (TauCl), an endogenous anti-inflammatory and antioxidative substance, is produced by reaction between taurine and hypochlorous acid by myeloperoxidase activity in neutrop...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2018.02.003

    authors： Kim W,Kim SH,Jang JH,Kim C,Kim K,Suh YG,Joe Y,Chung HT,Cha YN,Surh YJ

    更新日期：2018-05-01 00:00:00

  • Complement deposition on immune complexes reduces the frequencies of metabolic, proteolytic, and superoxide oscillations of migrating neutrophils.

    abstract：:Neutrophils exhibit intrinsic sinusoidal metabolite concentration oscillations of 3 min in resting cells and an additional approximately 10- or 20-s oscillation in migrating/adhering cells. To better understand immune complex (IC)-mediated leukocyte activation, we have studied neutrophil metabolic oscillations in the ...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1999.1481

    authors： Amit A,Kindzelskii AL,Zanoni J,Jarvis JN,Petty HR

    更新日期：1999-05-25 00:00:00

  • The 2H4 molecule but not the T3-receptor complex is involved in suppressor inducer signals in the AMLR system.

    abstract：:It is suggested that autologous mixed lymphocyte reaction (AMLR) may play an important role in generating suppressor inducer signals and in down-regulating the immune response following self-major histocompatibility recognition. In the present study, monoclonal antibodies directed at cell surface structures on T4+ cel...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(87)90270-x

    authors： Takeuchi T,Schlossman SF,Morimoto C

    更新日期：1987-06-01 00:00:00

  • Purification and characterization of two forms of rat interleukin-2.

    abstract：:Rat IL-2 produced by spleen cells in culture with concanavalin A was purified using gel filtration, hydrophobic chromatography, and ion-exchange chromatography. At least two forms of rat IL-2 were found to be separable by ion-exchange chromatography. These two forms have been designated form I and form II. Form I of r...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(88)90251-1

    authors： Thompson LP,Di Sabato G

    更新日期：1988-06-01 00:00:00

  • The CD8beta ectodomain contributes to the augmented coreceptor function of CD8alphabeta heterodimers relative to CD8alphaalpha homodimers.

    abstract：:Within the lymphoid compartment, CD8 is expressed either as an alphaalpha homodimer or as an alphabeta heterodimer. Prior functional characterization of CD8alpha transfectants has demonstrated that CD8alphaalpha homodimers can reconstitute T cell responses in the absence of the CD8beta subunit. In order to now examine...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1006/cimm.1998.1412

    authors： Witte T,Spoerl R,Chang HC

    更新日期：1999-02-01 00:00:00

  • The choriocarcinoma cell line JEG-3 upregulates regulatory T cell phenotypes and modulates pro-inflammatory cytokines through HLA-G.

    abstract：:An understanding of the interactions between immune cells and trophoblast cells, as well as choriocarcinoma cells, are of extreme importance in reproductive immunology and cancer immunology. In this study, we found that the human HLA-G-positive choriocarcinoma cell line JEG-3 upregulates CD4+CD25hiCD127lo T cells, inc...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/j.cellimm.2017.11.008

    authors： Melsted WN,Matzen SH,Andersen MH,Hviid TVF

    更新日期：2018-02-01 00:00:00

  • T helper cell lines that augment in vivo cytotoxic T-cell responses to minor alloantigens.

    abstract：:We have investigated the ability of long-term cultured T helper (Th) cell lines to help an in vivo cytotoxic T lymphocyte (CTL) response to non-H-2 alloantigens (minor antigens). Th cell lines specific for various single or undefined minor antigens were selected by regular restimulation with antigen in vitro. They wer...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(84)90309-5

    authors： Gascoigne NR,Lai PK

    更新日期：1984-02-01 00:00:00

  • The challenges of checkpoint inhibition in the treatment of multiple myeloma.

    abstract：:Despite significant improvements in the overall survival of patients with multiple myeloma (MM) over the past 15 years, the disease remains incurable. Treatment options are limited for patients who have relapsed or are refractory to immunomodulatory drugs (IMiDs), proteasome inhibitors, and monoclonal antibodies. In t...

    journal_title：Cellular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellimm.2018.10.003

    authors： Paul B,Kang S,Zheng Z,Kang Y

    更新日期：2018-12-01 00:00:00

  • Sequential analysis of experimental autoimmune thyroiditis induced by neonatal thymectomy in the Buffalo strain rat.

    abstract：:We have studied the evolution of thyroiditis induced by neonatal thymectomy in Buffalo strain rats, with particular emphasis on the thyroid lymphocytic infiltrate. The earliest change was increased endothelial Ia expression, and infiltration of the thyroid at 5 weeks by ED1- and ED2-positive macrophages and B and T ce...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(88)90260-2

    authors： Cohen SB,Dijkstra CD,Weetman AP

    更新日期：1988-06-01 00:00:00

  • Hemorrhage in mice produces alterations in intestinal B cell repertoires.

    abstract：:Abnormalities in immune response play a major role in the increased susceptibility to infection after hemorrhage and trauma. Infections occurring after injury often originate in the intestine. In order to determine the effects of hemorrhage on intestinal B cell function, we examined hemorrhage-induced alterations in a...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(90)90015-j

    authors： Abraham E,Chang YH

    更新日期：1990-06-01 00:00:00

  • A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.

    abstract：:Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and greater than 95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates ...

    journal_title：Cellular immunology

    pub_type： 杂志文章

    doi：10.1016/0008-8749(92)90212-8

    authors： Dato ME,Wierda WG,Kim YB

    更新日期：1992-04-01 00:00:00