Abstract：
:Rat IL-2 produced by spleen cells in culture with concanavalin A was purified using gel filtration, hydrophobic chromatography, and ion-exchange chromatography. At least two forms of rat IL-2 were found to be separable by ion-exchange chromatography. These two forms have been designated form I and form II. Form I of rat IL-2 was purified by a factor of 1297 and found to have a pI of 6.4. Form II was purified by a factor of 669 and found to have a pI between 5.4 and 6.1. Lectin chromatography was used to demonstrate that these two forms most likely differ in the extent of glycosylation. In the presence of tunicamycin the production of form II was significantly reduced. The two forms of rat IL-2 differ in their abilities to promote a mixed-lymphocyte reaction. Their differences in glycosylation may be the reason for these differences in activity.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Thompson LP,Di Sabato Gdoi
10.1016/0008-8749(88)90251-1subject
Has Abstractpub_date
1988-06-01 00:00:00pages
12-26issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(88)90251-1journal_volume
114pub_type
杂志文章abstract：:The proliferative responses of natural killer (NK) cells to 12-O-tetradecanoylphorbol-13-acetate (TPA), which directly activates protein kinase c(PKC), and to the Ca2+ ionophores A23817 and ionomycin, known to enhance the intracellular calcium, have been investigated. Highly purified large granular lymphocytes (LGL) w...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90007-x
更新日期：1988-04-15 00:00:00
abstract：:During the gestational cycle the placental tissue does not express class II MHC antigens and whether this phenomenon is important to fetal survival has not yet been evoked. It has been reported that class II antigen expression precedes renal and cardiac graft rejection, which may also be the case in fetal abortion. In...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90039-t
更新日期：1990-07-01 00:00:00
abstract：AIMS:Evaluate the participation of IL-17 pathway in T1D pathogenesis. T helper 17 cells are potent, highly inflammatory cells that produce interleukin 17A (IL-17A), considered a mediator of various immune disorders. However, their role in Type 1 diabetes (T1D) pathogenesis in humans is not totally elucidated. METHODS:...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2017.11.005
更新日期：2018-02-01 00:00:00
abstract：:Prior studies have shown that intraperitoneal (ip) injection of 25 IU of human rIL-2 can effectively modulate in vivo immune reactivity to thymus-dependent and thymus-independent type 2 immunogens in Xenopus laevis, the South African clawed toad, but is less successful at affecting toad cells in vitro. Here we compare...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1224
更新日期：1994-08-01 00:00:00
abstract：:Mice infected with virulent Mycobacterium tuberculosis exhibit an age-related increase in susceptibility to disease. The basis of this susceptibility has previously been shown to reflect an inability of the aged host to generate protective CD4 T cells during the early course of the infection. The results of the presen...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1062
更新日期：1993-03-01 00:00:00
abstract：:The pim-1 gene encodes a serine/threonine protein kinase with expression restricted primarily to cells of hematopoietic lineage and is thought to play a role in the signal transduction events associated with lymphocyte activation. A rapid increase in pim-1 mRNA levels was found after stimulation of normal unseparated ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1059
更新日期：1995-04-15 00:00:00
abstract：:Osteopontin (OPN) expression in tumors is associated with more aggressive tumor growth; however, several studies have suggested that OPN as a host protein can regulate tumor growth as well. OPN is produced by macrophages and T cells, and reportedly modifies macrophage function. Here, we have investigated the effect of...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2004.01.001
更新日期：2004-01-01 00:00:00
abstract：:Human skin contains epidermal Langerhans cells (LCs) and dermal dendritic cells (DCs) that are key players in induction of adaptive immunity upon infection. After major burn injury, suppressed adaptive immunity has been observed in patients. Here we demonstrate that burn injury affects adaptive immunity by altering bo...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.01.007
更新日期：2011-01-01 00:00:00
abstract：:NK activity is regulated by arachidonic acid metabolites. More precisely PGE2 and LTB4 decreases and increases respectively non-MHC-restricted cytotoxicity in humans. We have observed similar data in mice since NK activity was inhibited by PGE2 (10(-6) to 10(-8) M) and enhanced by LTB4 (10(-8) to 10(-12) M). On the ot...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90117-8
更新日期：1992-01-01 00:00:00
abstract：:Extracellular adenine nucleotides are inhibitors of the human natural killer cell line NK3.3 natural cytotoxicity activity. Natural cytotoxicity was inhibited approximately 26% by 1 mM ATP and 21% by 1 mM ADP. 5'-Adenylyl imidodiphosphate, a nonhydrolyzable ATP analog, inhibited natural cytotoxicity by 41% at a concen...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(95)80028-h
更新日期：1995-02-01 00:00:00
abstract：:The MCA 102 sarcoma has been defined by a variety of immunologic studies as a tumor lacking intrinsic immunogenicity. Nevertheless, we have recently demonstrated the feasibility of generating therapeutically effective lymphocytes for adoptive immunotherapy of this tumor. Procedures to achieve this required in vivo pri...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90201-2
更新日期：1990-08-01 00:00:00
abstract：:Recently we have shown that the generation of IFN-gamma-producing cells (IFN-gamma pc) were severely suppressed in cultures of Con A-stimulated splenocytes obtained from HgCl2-exposed BN but not Lewis rats. Since BN rats develop a TH2-biased polyclonal autoimmune syndrome upon exposure to HgCl2, whereas Lewis rats exh...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1027
更新日期：1995-04-01 00:00:00
abstract：:The antigenic phenotype of individual PHA-induced T lymphocyte colonies was studied with a direct immunofluorescence technique using fluorescein-labeled anti-Leu-2a and anti-Leu-3a antibodies. Of the colonies grown from mononuclear peripheral blood cells 85% were Leu-3a+ (inducer/helper phenotype), 12% were Leu-2a+ (s...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90235-6
更新日期：1983-10-15 00:00:00
abstract：:Functional helper and suppressor hybridomas were constructed from fractionated Type II NFR/nude mouse splenocytes which resemble precursors of T cells. After carrier priming with rabbit erythrocytes (RE), splenocytes from Type II NFR/nude mice were fractionated on nylon wool columns. Cells from two distinct fractions ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90329-0
更新日期：1984-12-01 00:00:00
abstract：:Ferric ammonium citrate (FAC) is nonmitogenic for human peripheral blood mononuclear cells (PBM) but has a potent mitogenic activity in the presence of IL-2. FAC in the presence of IL-2 increases the number of human peripheral blood mononuclear cells (PBM) expressing receptors for IL-2 and transferrin. FAC also marked...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90105-k
更新日期：1991-04-01 00:00:00
abstract：:An Affymetrix mouse genome array and differential in-gel electrophoresis (DIGE) techniques were used to investigate the pharmacological mechanisms of a mixture of herbs, designated CTCM, a compound of traditional Chinese medicine, for the treatment of increased permeability in mouse intestinal microvascular endothelia...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.06.004
更新日期：2010-01-01 00:00:00
abstract：:The primary function of the thymus is to develop immature T-cells into cells that further in the periphery will be able to carry out immune functions. The Literature has shown that thymus can be a target for many pathogens and severe structural alterations take place in this organ during infectious diseases. Here, we ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.06.001
更新日期：2008-05-01 00:00:00
abstract：:The transfer of B cells from mice immunized with Type III pneumococcal polysaccharide (SSS-III) results in the activation of suppressor and amplifier T cells that control the magnitude of the antibody response in recipient mice, immunized subsequently with SSS-III. Prior treatment of transferred B cells with an excess...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90368-5
更新日期：1985-12-01 00:00:00
abstract：:The pre-B cell stage of B lymphocyte development is characterized by the presence of immunoglobulin heavy chains of the IgM isotype in the cytoplasm and no other heavy or light chains in the cytoplasm or on the surface. We established several cell lines that were identical in their serologically defined pre-B cell phe...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90253-0
更新日期：1989-04-01 00:00:00
abstract：:The purpose of this study was to determine whether multiple types of suppressor factors play a role in the regulation of immune responses by ultraviolet radiation-induced suppressor T lymphocytes (UV Ts). The UV Ts were induced by applying contact allergens to the ventral, unirradiated skin of mice exposed 5 days earl...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90007-5
更新日期：1989-06-01 00:00:00
abstract：OBJECTIVE:Many CD95-expressing cells don't always undergo apoptosis after stimulation with CD95 ligation. The purpose of this paper is to investigate the role of expression of CD95 (Fas/Apo1) on inflammatory response in fibroblast-like synoviocytes (FLS) obtained from rheumatoid arthritis (RA) and to evaluate the role ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2014.07.004
更新日期：2014-08-01 00:00:00
abstract：:Oxidants, heavy metals, and heat shock, collectively known as stress stimuli, induce the synthesis of a variety of proteins, termed stress proteins, and enhance glucose uptake. In this study, we have demonstrated that stress stimuli enhance protein tyrosine phosphorylation (PTyr-P), modulate protein tyrosine phosphata...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90319-k
更新日期：1992-11-01 00:00:00
abstract：:Although CD4(+)CD25(+) regulatory T cells are pivotal in the suppression of autoimmunity, little is known about the effect of antigen-specific regulatory T cells on the formation of atheromatous plaques. Here, we describe the induction of heat-shock protein 60 (HSP60)-specific CD4(+)CD25(high) T cells by rapamycin (RP...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.01.002
更新日期：2006-10-01 00:00:00
abstract：:A monoclonal anti-actin antibody, 2C9, was used to study the distribution of an actin-like cell-surface antigen (hereafter termed actin) on a lymphoblastoid cell line LA350 and on human peripheral blood lymphocytes. It was determined that 8-40% of LA350 cells and 3-15% of peripheral blood lymphocytes from healthy dono...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90296-0
更新日期：1986-04-01 00:00:00
abstract：:We have established a long-lasting heart allograft tolerance in H-2-incompatible combinations by intravenous injection of 10(8) allogeneic spleen cells followed by the administration of 200 mg/kg of cyclophosphamide (CP). When C57BL/10 mice (B10, H-2b) were primed iv with 1 x 10(8) viable spleen cells from naive BALB/...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1142
更新日期：1994-05-01 00:00:00
abstract：:Experimental autoimmune encephalomyelitis was induced in a Lewis rat by sensitization with synthetic peptide GP68-86, representing the 68-86 sequence of guinea pig myelin basic protein (GPMBP). To delineate T cell determinants of GP68-86, lymph node cells from this rat were activated in culture with GP68-86 and were f...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90099-3
更新日期：1989-09-01 00:00:00
abstract：:The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we com...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90083-9
更新日期：1987-04-01 00:00:00
abstract：:Neutrophils exhibit intrinsic sinusoidal metabolite concentration oscillations of 3 min in resting cells and an additional approximately 10- or 20-s oscillation in migrating/adhering cells. To better understand immune complex (IC)-mediated leukocyte activation, we have studied neutrophil metabolic oscillations in the ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1999.1481
更新日期：1999-05-25 00:00:00
abstract：:The effects of oral administration of methylated bovine serum albumin (mBSA) on antigen-induced arthritis (AIA) in rats that was induced by immunization with mBSA followed by the intraarticular injection of the same antigen were investigated. When mBSA was orally given to animals before immunization with the antigen, ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1098
更新日期：1995-06-01 00:00:00
abstract：:Our previous studies show that growth hormone overexpression (GHo) upregulates the expression of the IGF-1R and IGF-2R resulting in the protection of the EL4 lymphoma cell line from apoptosis. In this study, we report that GHo also increases TGF-beta1 protein expression measured by luciferase promoter assay, Western a...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.06.003
更新日期：2006-03-01 00:00:00
