Abstract:
:TH1 cytokines have recently been detected in rheumatoid arthritis (RA) and osteoarthritis (OA). For this reason we studied the TH-1-promoting cytokine IL-12 in synovial membranes from patients with RA and OA. IL-12 transcripts and protein were analyzed by reverse transcriptase-polymerase chain reaction (PCR) and immunohistochemistry, respectively. In addition, IL-12 transcripts were quantitated by competitive PCR. IL-12 transcripts (p40) were detected in 8 of 13 patients with RA and in 10 of 18 patients with OA. Their levels did not differ significantly between RA and OA. IL-12 heterodimer protein was detected by immunostaining using an anti-IL-12p70 mAb. Double labeling with anti-IL-12p70 and anti-CD68 mAbs showed that synovial lining cells and monocytes/macrophages expressed IL-12 p70 protein. The presence of IL-12 p70 protein in the synovial membranes of patients with RA and OA suggests that IL-12 may play an important immunoregulatory role in these diseases by perpetuating inflammation.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Sakkas LI,Johanson NA,Scanzello CR,Platsoucas CDdoi
10.1006/cimm.1998.1363subject
Has Abstractpub_date
1998-09-15 00:00:00pages
105-10issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(98)91363-6journal_volume
188pub_type
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