Abstract:
:The voltage gated potassium channel (Kv1.3) has been shown to play a role in immune responsiveness. Blockade of the channel led to diminution of T cell activation and delayed type hypersensitivity. Previous in vitro studies of the blockade were focused on T cell activation and proliferation. In this study we examined other T and monocytic cell mediated events to glean the extent of the immunosuppressive effects of a Kv1.3 specific inhibitor, Margatoxin (MgTX). We found that MgTX inhibited the intracellular production of Th-1 as well as Th-2 cytokines. MgTX can also inhibit IL-2 production and proliferation of T cells upon stimulation with anti-CD3 and VCAM-1. Furthermore, a redirected cytolytic activity was also inhibited by MgTX. However, MgTX did not inhibit generation of CTL to EBV transformed lymphoma cells or antibody-dependent cellular cytolysis mediated by monocytes. It appears that a Kv1.3 blockade does not affect all immune responses, particularly those of innate immunity.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Shah K,Tom Blake J,Huang C,Fischer P,Koo GCdoi
10.1016/s0008-8749(03)00063-7subject
Has Abstractpub_date
2003-02-01 00:00:00pages
100-6issue
2eissn
0008-8749issn
1090-2163pii
S0008874903000637journal_volume
221pub_type
杂志文章abstract::MicroRNAs (miRNAs) are aberrant in many human tumors which can be transferred to immune cells by tumor-derived exosomes. Dendritic cells (DCs) play an important role in activation of immune response. However, the effect of tumor-derived exosomes on toll-like receptor (TLR) in DCs remains unclear. We investigated the i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2014.09.004
更新日期:2014-11-01 00:00:00
abstract::In Jurkat cells, the three Ca(2+)-ATPase blockers, thapsigargin, cyclopiazonic acid, and di-tert-butylhydroquinone (DtBuHQ) induced both a release of Ca2+ from intracellular stores and a Ca2+ influx. In contrast to CD3 mAb, the Ca(2+)-ATPase inhibitors did not induce the formation of inositol trisphosphate from the hy...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1152
更新日期:1993-07-01 00:00:00
abstract::Recently we have shown that the generation of IFN-gamma-producing cells (IFN-gamma pc) were severely suppressed in cultures of Con A-stimulated splenocytes obtained from HgCl2-exposed BN but not Lewis rats. Since BN rats develop a TH2-biased polyclonal autoimmune syndrome upon exposure to HgCl2, whereas Lewis rats exh...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1027
更新日期:1995-04-01 00:00:00
abstract::CD8+ T cell differentiation is controlled by the transcription factors T-bet and Eomesodermin, in concert with the cytokines IL-2, IL-10 and IL-12. Among these pathways, the mechanisms by which T-box proteins and IL-10 interact to promote a memory T cell fate remain poorly understood. Here, we show that Eomes and IL-1...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.11.008
更新日期:2019-01-01 00:00:00
abstract::The role of macrophages (M phi) in the in vitro primary antibody response of murine lymphocytes to sheep erythrocytes was investigated. Peritoneal M phi were activated to express Ia antigens either in vitro or in vivo. Nonactivated Ia- M phi were also examined. We observed that only Ia- M phi but also Ia+ M phi failed...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90316-8
更新日期:1985-10-15 00:00:00
abstract::Patients treated with monoclonal antibodies and cytokines for cancer receive often co-medication, which may influence treatment efficacy. Therefore, we investigated with a flowcytometric cytotoxicity assay the effect of several immunomodulatory drugs on antibody dependent cellular cytotoxicity (ADCC), interleukin-2 (I...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.05.002
更新日期:2007-03-01 00:00:00
abstract::Tumour necrosis factor (TNF) family ligands and their corresponding receptors play important roles in the immune system and are involved in immune regulation such as lymphoid development, cell proliferation, differentiation, activation and death. Antibodies against these ligands and receptors together with Fc-fusion p...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.08.010
更新日期:2005-07-01 00:00:00
abstract::Mycobacterial antigens including BCG stimulate human peripheral blood mononuclear cells resulting in cellular proliferation and the release of inflammatory cytokines such as TNF-alpha. However, the signal transduction mechanisms responsible for the BCG-induced cell activation are not completely understood. In this stu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0185
更新日期:1996-07-10 00:00:00
abstract::Although pulmonary inflammation is a serious, sometimes life-threatening, consequence of respiratory syncytial virus (RSV) infection, the mechanisms involved are not well understood. Since the process of inflammation is initiated by a complex series of events including the activation of specific adhesion molecules on ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00058-3
更新日期:2003-01-01 00:00:00
abstract::Previous studies have demonstrated that murine thymocytes proliferate in the presence of submitogenic concentrations of phytohemagglutinin-P (PHA-P) and various cytokines such as interleukin-1 (IL-1), interleukin-4 (IL-4), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6). We report that C3H/HeJ thymoc...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90253-n
更新日期:1990-12-01 00:00:00
abstract::Anti-TNF-α therapies have been applied in RA treatment, but the regulatory effect of the drug on immune system is not clear. In this study, we included 33 active RA patients and divided them into two groups. One group received anti-TNF-α mAb+methotrexate for 24 weeks, the other group got placebo+methotrexate for the f...
journal_title:Cellular immunology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.cellimm.2012.09.001
更新日期:2012-09-01 00:00:00
abstract::During normal lymphocyte recirculation and in chronic inflammation, lymphocytes emigrate from blood into the perivascular tissue. The mechanism of lymphocyte migration through the endothelial cell (EC) layer of blood vessels is poorly understood. To identify factors that control lymphocyte emigration, a method has bee...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90324-3
更新日期:1988-07-01 00:00:00
abstract::Intravenous injection of spleen cells (SC) coated with an antitrinitrophenyl (anti-TNP) IgM monoclonal antibody, Sp6 (Sp6-SC), which carries a recurrent idiotype, resulted in activation of a Lyt-2-positive population which did adhere to Sp6-coated plates. No effect of Sp6-SC injection could be observed in vivo on an a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90314-x
更新日期:1986-04-01 00:00:00
abstract::Stimulated macrophages are known to inhibit the growth of certain tumor cells. Using mouse peritoneal exudates as a source of macrophages and the mastocytoma cell line P815 as the target, the inhibition was found to depend on direct contact between the macrophages and the growing cells. Cytostatic activities were dete...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90395-3
更新日期:1986-02-01 00:00:00
abstract::Altered neutrophil function may contribute to the development of AIDS during the course of HIV infection. It has been described that Nef, a regulatory protein from HIV, can modulate superoxide production in other cells, therefore altered superoxide production in neutrophils from HIV infected patients, could be seconda...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.03.012
更新日期:2010-01-01 00:00:00
abstract::Macrophages are highly plastic immune cells with temporally distinct transcriptome changes upon lipopolysaccride (LPS) activation. However, to what extent transcriptome reprogramming is mediated via spatial chromatin looping is not well studied. We generated high resolution chromatin interaction maps for LPS-stimulate...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104148
更新日期:2020-09-01 00:00:00
abstract::Immunization with synthetic mRNA encoding tumor-associated antigens is an emerging vaccine strategy for the treatment of cancer. In order to prevent mRNA degradation, promote antigen-presenting cells antigen presentation, and induce an anti-tumor immune response, we investigated the nasal administration of mRNA vaccin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104143
更新日期:2020-08-01 00:00:00
abstract::Peripheral blood polymorphonuclear neutrophils (PMN) from mice immunized with Blastomyces dermatitidis and then stimulated locally (intraperitoneally, ip) with B. dermatitidis antigen had enhanced killing of B. dermatitidis in vitro (54.4 +/- 19.49 of inoculum) compared to nonimmune mice (32.7 +/- 8.7%; P less than 0....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90111-0
更新日期:1987-11-01 00:00:00
abstract::Interleukin (IL)-35 strongly suppresses the immune effects of CD8+ T cells. However, the mechanisms mediating these effects are not clear. Here, we investigated the potential inhibitory mechanisms of IL-35 using proteomics technology. The changes of differentially expressed proteins (DEPs) were evaluated using gene on...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.104022
更新日期:2020-02-01 00:00:00
abstract::Chitin oligosaccharides (NA-COS) of two different molecular weight ranges (below 1 and 1-3 kDa) were examined for their capabilities against lipopolysaccharide-induced inflammatory responses in BV-2 murine microglia. It was found that NA-COS reduced the level of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) produc...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.06.005
更新日期:2012-05-01 00:00:00
abstract::The lymphokine IL-4 is a potent enhancer of anti-IgM-induced B cell proliferation. Although the mechanism of this enhancement is not known, a commonly held view suggests that IL-4 acts together with anti-IgM as a costimulating factor for the activation of a subpopulation of B cells. To evaluate this hypothesis we exam...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90327-8
更新日期:1991-04-15 00:00:00
abstract::Cells from the spleen, lymph nodes, and peritoneum of DBA/2 mice bearing a subcutaneous tumor mediate nonspecific suppression of an in vitro antibody response to sheep red blood cells (SRBC) when cocultured with a normal T-cell subset(s). The spleen cells from the tumor-bearing mouse required for the suppression bear ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90047-3
更新日期:1983-07-01 00:00:00
abstract::We have previously demonstrated that natural killer (NK) cells are lysosome-rich and stain more intensely with lysosomotropic agents such as neutral red and quinacrine (Qu) than do non-NK cells. In this study we combined the quantitation of Qu staining with surface marker staining to define subpopulations of NK cells....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90158-x
更新日期:1988-08-01 00:00:00
abstract::Two functional T hybridomas were prepared by fusing BW5147 with ovalbumin (OVA)-primed splenic T blast cells. One was OVA specific for helper function requiring concanavalin A supernatant (CAS) for activity while the other, termed autoreactive, was nonspecific for helper-augmenting activity. Both required H-2d present...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90371-x
更新日期:1984-06-01 00:00:00
abstract::Blockade of the immune cell checkpoint inhibitors programmed death 1 (PD-1) and programmed death-ligand 1 (PD-L1) has become a powerful tool in cancer treatment, which is effective across various solid cancer types and hematologic malignancies. Our previous studies showed that by reducing immune tolerance, clustered r...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.09.003
更新日期:2017-10-01 00:00:00
abstract::C3H/HeJ mice are homozygous for the Lpsd allele and, as a consequence, are hyporesponsive to all of the biological effects of bacterial lipopolysaccharide (LPS) that have been studied. These mice die in the early phase of infection when inoculated with virulent Salmonella. This susceptibility is also regulated by the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90326-6
更新日期:1986-10-01 00:00:00
abstract::PIM kinases are a family of three serine/threonine kinases expressed following T cell activation. Using potent selective small molecule antagonists of PIM-1/3 kinases, we demonstrate a potential role for these enzymes in naïve and effector CD4+ T cell activation. PIM-1/3 inhibition prevented CD4+ T cell proliferation ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.10.011
更新日期:2012-01-01 00:00:00
abstract::We previously reported that T cell proliferation in response to a primary signal through the T cell receptor (TCR) and a costimulatory signal via the CD28 molecule is impaired in healthy, aged mice. Here we extend these studies to examine factors which may be involved in this defect in T cells from aged mice. To deter...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0144
更新日期:1996-05-25 00:00:00
abstract:AIMS:Evaluate the participation of IL-17 pathway in T1D pathogenesis. T helper 17 cells are potent, highly inflammatory cells that produce interleukin 17A (IL-17A), considered a mediator of various immune disorders. However, their role in Type 1 diabetes (T1D) pathogenesis in humans is not totally elucidated. METHODS:...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.11.005
更新日期:2018-02-01 00:00:00
abstract::The aim of the study was to investigate the interaction between manLAM and DC-SIGN influencing DCs maturation and downstream immune response using small interfering RNA-expressing lentiviral vectors to specifically knockdown DC-SIGN. Our data indicated that DC-SIGN knockdown alone in DCs did not affect the maturation ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.09.001
更新日期:2011-01-01 00:00:00