Pancreatic cancer derived exosomes regulate the expression of TLR4 in dendritic cells via miR-203.

Abstract:

:MicroRNAs (miRNAs) are aberrant in many human tumors which can be transferred to immune cells by tumor-derived exosomes. Dendritic cells (DCs) play an important role in activation of immune response. However, the effect of tumor-derived exosomes on toll-like receptor (TLR) in DCs remains unclear. We investigated the influence of pancreatic cancer derived exosomes on TLR4, and downstream cytokines via miR-203. Our results showed that miR-203 expressed in panc-1 cells and exosomes, and upregulated in exosomes-treated DCs. TLR4 decreased after treatment of exosomes and miR-203 mimics, while increased in exosomes-treated DCs by miR-203 inhibitors. But the mRNA level of TLR4 was not significantly different between DCs and exosomes-treated DCs. Tumor necrosis factor-α (TNF-α) and interleukin-12 (IL-12) also decreased under treatment of exosomes and miR-203 mimics, both of which increased in exosomes-treated DCs by miR-203 inhibitors. Collectively, pancreatic cancer derived exosomes downregulate TLR4 and downstream cytokines in DCs via miR-203.

journal_name

Cell Immunol

journal_title

Cellular immunology

authors

Zhou M,Chen J,Zhou L,Chen W,Ding G,Cao L

doi

10.1016/j.cellimm.2014.09.004

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

65-9

issue

1-2

eissn

0008-8749

issn

1090-2163

pii

S0008-8749(14)00147-6

journal_volume

292

pub_type

杂志文章
  • Prolongation of alloskin graft survival by catalytic scavengers of reactive oxygen species.

    abstract::We tested the effects of salen manganese (Salen-Mn) complexes, which are scavengers of reactive oxygen species exhibiting superoxide dismutase and catalase activities on the rejection of and alloresponse to fully allogeneic skin grafts in mice. We showed that pre-transplant treatment of C57Bl/6 donor skin or of BALB/c...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2006.06.009

    authors: Tocco G,Illigens BM,Malfroy B,Benichou G

    更新日期:2006-06-01 00:00:00

  • Relationship between MIF-173 G/C polymorphism and susceptibility to chronic hepatitis B and HBV-induced liver cirrhosis.

    abstract::Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, played an important role in immune-mediated diseases. The promoter region of MIF, which had functional polymorphisms, controlled MIF expression. MIF polymorphism was associated with many inflammatory diseases. But the association of MIF polymor...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2013.04.013

    authors: Zhang K,Pan X,Shu X,Cao H,Chen L,Zou Y,Deng H,Li G,Xu Q

    更新日期:2013-04-01 00:00:00

  • Toll-like receptor 3 is involved in airway epithelial cell response to nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is the etiological agent most frequently associated with bacterial exacerbations of chronic obstructive pulmonary disease (COPD). The present work shows that NTHi strains induced in primary normal human bronchial epithelial cells (NHBE) a cytokine/chemokine response in which C...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2009.09.008

    authors: Teng F,Slavik V,Duffy KE,San Mateo L,Goldschmidt R

    更新日期:2010-01-01 00:00:00

  • Semaphorin 4A is dynamically regulated during thymocyte development in mice.

    abstract::Semaphorins are important regulators of peripheral T and B-cell mediated immune responses in mice and humans. Modulatory roles of semaphorins in T cell development are also being characterized. We carefully analyzed the gene expression and protein levels of semaphorins 4A, 4D, and 7A at various developmental stages of...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2013.03.006

    authors: Linder GE,Chuntova PD,McLelland BT,Añó L,Obodo UC,Crider NJ,Matthes DJ,García-Ojeda ME,Manilay JO,Chatterjea D

    更新日期:2013-02-01 00:00:00

  • Effector phenotype and immunologic specificity of T-cell-mediated adoptive therapy for a murine tumor that lacks intrinsic immunogenicity.

    abstract::The MCA 102 sarcoma has been defined by a variety of immunologic studies as a tumor lacking intrinsic immunogenicity. Nevertheless, we have recently demonstrated the feasibility of generating therapeutically effective lymphocytes for adoptive immunotherapy of this tumor. Procedures to achieve this required in vivo pri...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(90)90201-2

    authors: Sakai K,Chang AE,Shu S

    更新日期:1990-08-01 00:00:00

  • Natural cytotoxic T cells (NCTC) that differ from natural killer (NK) and natural cytotoxic (NC) cells are present in Peyer's patches of mice.

    abstract::We have examined noninduced cytotoxicity of mouse gut associated and peripheral lymphoid tissues for a wide variety of syngeneic as well as allogeneic cell lines and lymphoblasts. Lymphoid cells from Peyer's patches were found to lyse these targets in a 3-hr chromium release assay whereas lymphoid cells from intestina...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(86)90158-9

    authors: Gautam SC,Beckman K,Battisto JR

    更新日期:1986-09-01 00:00:00

  • Effects of modulators of adenylyl cyclase on interleukin-2 production, cytosolic Ca2+ elevation, and K+ channel activity in Jurkat T cells.

    abstract::We have studied the effects of prostaglandin E2 (PGE2) and cholera toxin, two modulators of adenylyl cyclase, and 8-bromo cAMP (8-BrcAMP) on various parameters of lymphocyte activation using the human T cell line Jurkat. Our results show that PGE2 and cholera toxin inhibit, in a dose-related manner, the phytohemagglut...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(90)90035-p

    authors: Bastin B,Payet MD,Dupuis G

    更新日期:1990-07-01 00:00:00

  • Vascular and cellular events in post-mastectomy seroma: an immunohistochemical study.

    abstract::This study aimed to describe the vascular and cellular histopathological changes that occurred in post-mastectomy seroma in an animal model. Unilateral mastectomies were conducted on 45 female albino rabbits. On day seven, the skin flap and the underlying tissues of the mastectomy regions were dissected and processed ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2011.10.021

    authors: Al-Gaithy ZK,Ayuob NN

    更新日期:2012-01-01 00:00:00

  • Modulation of granulomatous inflammation in murine schistosomiasis mansoni by enteric exposure to schistosome ova: in vitro characterization of a regulatory mechanism within the granuloma.

    abstract::Enteric immunization with schistosome ova results in a diminished granulomatous response. This study explored a mechanism by which enteric immunization may decrease granuloma size. Granulomas from livers of acutely infected mice were dissociated and the dispersed cells were depleted of macrophages. As defined by a dir...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(87)90227-9

    authors: Weinstock JV,Blum AM

    更新日期:1987-09-01 00:00:00

  • A comparison of the effects of human rIL-2 and autologous TCGF on Xenopus laevis splenocytes in vitro.

    abstract::Prior studies have shown that intraperitoneal (ip) injection of 25 IU of human rIL-2 can effectively modulate in vivo immune reactivity to thymus-dependent and thymus-independent type 2 immunogens in Xenopus laevis, the South African clawed toad, but is less successful at affecting toad cells in vitro. Here we compare...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1994.1224

    authors: Ruben LN,Rak JC,Johnson RO,Shiigi S,Clothier RH

    更新日期:1994-08-01 00:00:00

  • Noradrenaline through β-adrenoceptor contributes to sexual dimorphism in primary CD4+ T-cell response in DA rat EAE model?

    abstract::Males exhibit stronger sympathetic nervous system (SNS) activity, but weaker primary CD4+ T-cell (auto)immune responses. To test the role of catecholamines, major end-point SNS mediators, in this dimorphism, influence of propranolol (β-adrenoceptor blocker) on mitogen/neuroantigen-stimulated CD4+ T cells from female a...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2018.12.009

    authors: Vujnović I,Pilipović I,Jasnić N,Petrović R,Blagojević V,Arsenović-Ranin N,Stojić-Vukanić Z,Djordjević J,Leposavić G

    更新日期:2019-02-01 00:00:00

  • Lack of detectable immunoglobulin E receptor expression on 33 of 34 cell lines with natural killer-like or cytotoxic-T-lymphocyte activity.

    abstract::We recently reported that a cloned murine cell line with "natural killer (NK)-like" cytolytic function and prominent cytoplasmic granules also expressed large numbers of plasma membrane receptors (Fc epsilon R) which bound mouse immunoglobulin E (IgE) with high affinity (S.J. Galli et al., 1982, Nature (London) 298, 2...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(85)90353-3

    authors: Galli SJ,Brooks CG,Dvorak AM,Ishizaka T

    更新日期:1985-11-01 00:00:00

  • Class I-like CD1A-C do not protect target cells from NK-mediated cytolysis.

    abstract::Previous studies have validated the capacity of classical (HLA-A,B,C) and nonclassical (HLA-G) MHC class I antigens expressed by target cells to influence natural killer (NK) cell-mediated cytolysis. Generally, elevated expression of these HLA class I molecules is correlated with enhanced resistance to lysis by NK cel...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1996.0020

    authors: Storkus WJ,Wei M,Cresswell P,Dawson JR

    更新日期:1996-01-10 00:00:00

  • Generation of a soluble IFN-gamma inducer by oxidation of galactose residues on macrophages.

    abstract::Depletion of macrophages from human peripheral blood mononuclear cells (PBMC) caused a marked decrease in galactose oxidase and sodium periodate, but not a calcium ionophore, stimulated Interferon-gamma (IFN-gamma) production. Reconstitution of such depleted cultures with galactose oxidase treated macrophages, but not...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(85)90268-0

    authors: Antonelli G,Blalock JE,Dianzani F

    更新日期:1985-09-01 00:00:00

  • Irreversible inhibition of human natural killer cell natural cytotoxicity by modification of the extracellular membrane by the adenine nucleotide analog 5'-p-(fluorosulfonyl)benzoyl adenosine.

    abstract::Extracellular adenine nucleotides are inhibitors of the human natural killer cell line NK3.3 natural cytotoxicity activity. Natural cytotoxicity was inhibited approximately 26% by 1 mM ATP and 21% by 1 mM ADP. 5'-Adenylyl imidodiphosphate, a nonhydrolyzable ATP analog, inhibited natural cytotoxicity by 41% at a concen...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(95)80028-h

    authors: Dombrowski KE,Cone JC,Bjorndahl JM,Phillips CA

    更新日期:1995-02-01 00:00:00

  • Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.

    abstract::In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.2001.1895

    authors: Schaefer BC,Schaefer ML,Kappler JW,Marrack P,Kedl RM

    更新日期:2001-12-15 00:00:00

  • Protective effect of chitin oligosaccharides against lipopolysaccharide-induced inflammatory response in BV-2 microglia.

    abstract::Chitin oligosaccharides (NA-COS) of two different molecular weight ranges (below 1 and 1-3 kDa) were examined for their capabilities against lipopolysaccharide-induced inflammatory responses in BV-2 murine microglia. It was found that NA-COS reduced the level of nitric oxide (NO) and prostaglandin E(2) (PGE(2)) produc...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2012.06.005

    authors: Vo TS,Ngo DH,Ta QV,Wijesekara I,Kong CS,Kim SK

    更新日期:2012-05-01 00:00:00

  • Inhibition of T cell activation and adhesion functions by soluble CD2 protein.

    abstract::The CD2 (T11) molecule belongs to a family of cell-surface glycoproteins that function as adhesion molecules in the immune system. Human CD2 is found exclusively on cells of the T lineage: peripheral T lymphocytes, NK cells, and thymocytes. CD2 binds specifically to the surface glycoprotein LFA-3. CD2/LFA-3 adhesion i...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1993.1133

    authors: Rabin EM,Gordon K,Knoppers MH,Luther MA,Neidhardt EA,Flynn JF,Sardonini CA,Sampo TM,Concino MF,Recny MA

    更新日期:1993-06-01 00:00:00

  • Effects of anti-CD4 antibody: enhancement of lymph node PPD-memory T cell response.

    abstract::In vitro incubations of CD4+ T cells with anti-CD4 monoclonal antibody (Mab) demonstrated saturation binding and inhibited both PPD-stimulated proliferation and delayed-type hypersensitivity (DTH) after passive transfer of these cells into naive rats. In comparison, in vivo administration of anti-CD4 Mab into rats, 2 ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1995.1104

    authors: Morrison WJ,Kennedy NJ,Offner H,Vandenbark AA

    更新日期:1995-06-01 00:00:00

  • "Cross-wiring" of the immune response in old mice: increased autoantibody response despite reduced antibody response to nominal antigen.

    abstract::Older humans and experimental animals have been repeatedly found to have higher titers of autoantibodies than do younger individuals despite the impaired responses of older individuals to foreign antigens. The studies reported here were designed to examine the relationship between these two age-related changes in anti...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(91)90294-l

    authors: Bovbjerg DH,Kim YT,Schwab R,Schmitt K,DeBlasio T,Weksler ME

    更新日期:1991-07-01 00:00:00

  • Anti-inflammatory properties of estrogen. I. In vivo suppression of leukocyte production in bone marrow and redistribution of peripheral blood neutrophils.

    abstract::We have recently demonstrated that estrogen has differential immunomodulatory properties in mice because it stimulates antibody responses but inhibits T cell-mediated inflammation. In the present study we have examined the influence of estrogen on T cell-independent inflammatory responses. A local inflammation was ind...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(92)90269-u

    authors: Josefsson E,Tarkowski A,Carlsten H

    更新日期:1992-06-01 00:00:00

  • Increased Zap-70 association with CD3zeta in CD4 T cells from old mice.

    abstract::Aging diminishes the amount of phosphotyrosine in the CD3zeta chains of resting and activated mouse CD4 T cells by about threefold and might therefore be expected to a corresponding decline in Zap-70 association with CD3zeta and in Zap-70 kinase function in CD3zeta complexes. We show here that aging leads, unexpectedl...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1998.1394

    authors: Garcia GG,Miller RA

    更新日期:1998-12-15 00:00:00

  • A thymic nurse cell-specific monoclonal antibody.

    abstract::A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1998.1279

    authors: Pezzano M,King KD,Philp DD,Adeyemi A,Gardiner B,Yang J,Samms M,Boto W,Guyden JC

    更新日期:1998-05-01 00:00:00

  • Androgens alter B cell development in normal male mice.

    abstract::Castration of normal male mice leads to splenic enlargement and expansion of the B cell population. Since the spleen does not express receptors for androgens, these changes are most likely mediated by effects of androgens on other target organs. Two potential sites of androgen-mediated effects on B cells are evaluated...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1997.1227

    authors: Viselli SM,Reese KR,Fan J,Kovacs WJ,Olsen NJ

    更新日期:1997-12-15 00:00:00

  • Sex differences and estrogen regulation of miRNAs in lupus, a prototypical autoimmune disease.

    abstract::Unique dysregulated expression patterns of microRNAs (miRNAs) have been reported in many disease conditions including autoimmune diseases such as systemic lupus erythematosus (SLE). We have recently reported that miRNAs are differentially expressed not only between autoimmune and control mice, but also between male an...

    journal_title:Cellular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cellimm.2015.01.004

    authors: Khan D,Dai R,Ansar Ahmed S

    更新日期:2015-04-01 00:00:00

  • CTLA-4 and autoimmune thyroid disease: lack of influence of the A49G signal peptide polymorphism on functional recombinant human CTLA-4.

    abstract::A single nucleotide A49G polymorphism (SNP) of CTLA-4 has been linked and associated with the autoimmune thyroid diseases (AITD) and thyroid autoantibody secretion. We have explored the functional mechanisms of CTLA-4 by means of recombinant human CTLA-4 expressed on transfected Jurkat T cells. Analysis of CTLA-4 tran...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/s0008-8749(02)00018-7

    authors: Xu Y,Graves PN,Tomer Y,Davies TF

    更新日期:2002-02-01 00:00:00

  • In vivo mechanisms of acquired thymic tolerance.

    abstract::Injection of antigen into the thymus of adult animals induces specific systemic tolerance, but the mechanisms of acquired thymic tolerance are not well understood. To investigate these mechanisms we used a model of intrathymic injection of ovalbumin (OVA) in BALB/c mice. We show an antigen-specific decrease in prolife...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1997.1165

    authors: Chen W,Issazadeh S,Sayegh MH,Khoury SJ

    更新日期:1997-08-01 00:00:00

  • Physiochemical characterization of lymphocyte inhibitory factor (LyIF) isolated from avian B and T cells.

    abstract::Thymic (T) or bursal (B) lymphocytes from chicks sensitized to Mycobacterium tuberculosis produce an avian lymphocyte inhibitory factor (LyIF). The physiochemical properties of both T and B LyIF were established by ultrafiltration which yielded four fractions with molecular weight ranges of greater than 100,000; 50,00...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/0008-8749(83)90080-1

    authors: Martin DE,Glick B

    更新日期:1983-07-15 00:00:00

  • Lifelong exposure to dietary fish oil alters macrophage responses in Walker 256 tumor-bearing rats.

    abstract::Supplementation of the diet with fish oil (FO) decreases growth of the Walker 256 tumor and decreases the cachexia associated with tumor-bearing. The mechanisms by which FO inhibits tumor growth and cachexia are unknown. Macrophages are very important in host defence against tumors since they produce several anti-tumo...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1016/j.cellimm.2004.12.001

    authors: Bonatto SJ,Folador A,Aikawa J,Yamazaki RK,Pizatto N,Oliveira HH,Vecchi R,Curi R,Calder PC,Fernandes LC

    更新日期:2004-09-01 00:00:00

  • Interleukin-12 (IL-12) augments cytolytic activity of natural killer cells toward Mycobacterium tuberculosis-infected human monocytes.

    abstract::We tested the impact of interleukin-12 (IL-12) and of cytotoxic leukocytes (particularly natural killer (NK) cells) from normal and HIV-1-infected subjects on lysis of human monocytes infected with Mycobacterium tuberculosis. Nylon wool nonadherent cells stimulated with interleukin-2 (IL-2) or with IL-12 developed sig...

    journal_title:Cellular immunology

    pub_type: 杂志文章

    doi:10.1006/cimm.1994.1196

    authors: Denis M

    更新日期:1994-07-01 00:00:00