Abstract:
:MicroRNAs (miRNAs) are aberrant in many human tumors which can be transferred to immune cells by tumor-derived exosomes. Dendritic cells (DCs) play an important role in activation of immune response. However, the effect of tumor-derived exosomes on toll-like receptor (TLR) in DCs remains unclear. We investigated the influence of pancreatic cancer derived exosomes on TLR4, and downstream cytokines via miR-203. Our results showed that miR-203 expressed in panc-1 cells and exosomes, and upregulated in exosomes-treated DCs. TLR4 decreased after treatment of exosomes and miR-203 mimics, while increased in exosomes-treated DCs by miR-203 inhibitors. But the mRNA level of TLR4 was not significantly different between DCs and exosomes-treated DCs. Tumor necrosis factor-α (TNF-α) and interleukin-12 (IL-12) also decreased under treatment of exosomes and miR-203 mimics, both of which increased in exosomes-treated DCs by miR-203 inhibitors. Collectively, pancreatic cancer derived exosomes downregulate TLR4 and downstream cytokines in DCs via miR-203.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Zhou M,Chen J,Zhou L,Chen W,Ding G,Cao Ldoi
10.1016/j.cellimm.2014.09.004subject
Has Abstractpub_date
2014-11-01 00:00:00pages
65-9issue
1-2eissn
0008-8749issn
1090-2163pii
S0008-8749(14)00147-6journal_volume
292pub_type
杂志文章abstract::We tested the effects of salen manganese (Salen-Mn) complexes, which are scavengers of reactive oxygen species exhibiting superoxide dismutase and catalase activities on the rejection of and alloresponse to fully allogeneic skin grafts in mice. We showed that pre-transplant treatment of C57Bl/6 donor skin or of BALB/c...
journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0020
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.06.005
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journal_title:Cellular immunology
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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