Effect of the antimicrobial peptide LL-37 on Toll-like receptors 2-, 3- and 4-triggered expression of IL-6, IL-8 and CXCL10 in human gingival fibroblasts.


:The antimicrobial peptide LL-37 is known to have a potent LPS-neutralizing activity in monocytes and macrophages. Recently, LL-37 in gingival crevicular fluids is suggested to be the major protective factor preventing infection of periodontogenic pathogens. In this study, we tried to address the effect of LL-37 on proinflammatory responses of human gingival fibroblasts (HGFs) stimulated with Toll-like receptor (TLR)-stimulant microbial compounds. LL-37 potently suppressed LPS-induced gene expression of IL6, IL8 and CXCL10 and intracellular signaling events, degradation of IRAK-1 and IkappaBalpha and phosphorylation of p38 MAPK and IRF3, indicating that the LPS-neutralizing activity is also exerted in HGFs. LL-37 also suppressed the expression of IL6, IL8 and CXCL10 induced by the TLR3 ligand poly(I:C). LL-37 modestly attenuated the expression of IL6 and IL8 induced by the TLR2/TLR1 ligand Pam(3)CSK(4), but did not affect the expression induced by the TLR2/TLR6 ligand MALP-2. Interestingly, LL-37 rather upregulated the expression of IL6, IL8 and CXCL10 induced by another TLR2/TLR6 ligand FSL-1. Thus, the regulatory effect of LL-37 is differently exerted towards proinflammatory responses of HGFs induced by different microbial stimuli, which may lead to unbalanced proinflammatory responses of the gingival tissue to infection of oral microbes.


Cell Immunol


Cellular immunology


Into T,Inomata M,Shibata K,Murakami Y




Has Abstract


2010-01-01 00:00:00














  • Functional role of self IA molecules in polyclonal B cell activation using an autoreactive B cell clone derived from (NZB X NZW) F1 mice.

    abstract::The mechanism of polyclonal B cell activation in autoimmune diseases was investigated by using an autoreactive B cell clone established by somatic hybridization with B cells derived from NZB X NZW (B/W) F1 mice. Briefly, splenic B cells from B/W F1 mice were fused with M12.4.1, a mutant of a B cell line, in the presen...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Iwasaki T,Hamano T,Hase K,Murata Y,Yamasaki T,Kakishita E

    更新日期:1992-02-01 00:00:00

  • Interleukin 2 production by human T lymphocytes identified by antibodies to dipeptidyl peptidase IV.

    abstract::T-Cell subsets identified by polyclonal and monoclonal antibodies to dipeptidyl peptidase IV (DP IV) were investigated. Analysis in a cytofluorograf revealed 63 +/- 7% positive scatter-gated T lymphocytes. DP IV-positive cells were found to be T11+, 74-81% OKT4+, and 12-19% OKT8+. DP IV-negative cells were T11+ and co...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Scholz W,Mentlein R,Heymann E,Feller AC,Ulmer AJ,Flad HD

    更新日期:1985-06-01 00:00:00

  • Evaluation of macrophage plasticity in brown and white adipose tissue.

    abstract::There are still questions about whether macrophage differentiation is predetermined or is induced in response to tissue microenvironments. C2D macrophage cells reside early in the macrophage lineage in vitro, but differentiate to a more mature phenotype after adoptive transfer to the peritoneal cavity (PEC-C2D). Since...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ortega MT,Xie L,Mora S,Chapes SK

    更新日期:2011-01-01 00:00:00

  • Lysis of herpes simplex virus-infected targets. II. Nature of the effector cells.

    abstract::Peripheral blood lymphocytes (PBL) from patients with herpes simplex virus (HSV)-1 recurrences in the cornea only (Group I) exhibited reduced lysis of HSV-1-infected targets compared to PBL from patients with oral-facial and corneal HSV recurrences (Group II). The cytotoxic lymphocytes appeared to belong to a subpopul...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Hendricks RL,Sugar J

    更新日期:1984-02-01 00:00:00

  • TGF-beta1 expression in EL4 lymphoma cells overexpressing growth hormone.

    abstract::Our previous studies show that growth hormone overexpression (GHo) upregulates the expression of the IGF-1R and IGF-2R resulting in the protection of the EL4 lymphoma cell line from apoptosis. In this study, we report that GHo also increases TGF-beta1 protein expression measured by luciferase promoter assay, Western a...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Farmer JT,Weigent DA

    更新日期:2006-03-01 00:00:00

  • Cellular immunity in allograft rejection: role of lymphocyte subpopulations and T-cell subsets in rat cardiac allograft rejection.

    abstract::In this study, cellular requirements for rejection are examined by the use of adoptive transfer assays in the ACI to Lewis cardiac allograft model. The findings show that adoptive transfer of 1 x 10(8) spleen cells (SpL), 5 x 10(7) T-cells, and 2 x 10(7) helper T-cells (W3/25+) obtained from normal, nonsensitized dono...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Oluwole SF,Engelstad K,Hardy MA

    更新日期:1989-11-01 00:00:00

  • Estrogen and antiestrogen modulation of the levels of mouse natural killer activity and large granular lymphocytes.

    abstract::We investigated the time course of the 17 beta-estradiol effect on mouse natural killer (NK) activity and the number of splenic large granular lymphocytes (LGL), a cell population recently associated with natural cytotoxicity and enriched in low density fractions of Percoll discontinuous density gradients. After 7 day...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Screpanti I,Santoni A,Gulino A,Herberman RB,Frati L

    更新日期:1987-05-01 00:00:00

  • Studies of the in vitro activation and differentiation of human B lymphocytes. II. Optimization of activation by anti-immunoglobulin antibody bound to beads: analysis of the role of autocrine effects on B-cell proliferation and of T-cell help in B-cell di

    abstract::We report experiments attempting to optimize the proliferative response of human B cells to rabbit anti-immunoglobulin antibody (RAHIg)-linked beads (anti-Ig beads). By choosing polyacrylamide beads of small size (3 micron) and coupling anti-Ig to them at high concentrations, beads were obtained which were both B-cell...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Boyd AW,Freedman AS,Horowitz JC,Anderson KC,Fisher DC,Rosen KJ,Schlossman SF,Nadler LM

    更新日期:1986-04-15 00:00:00

  • Functional defect of heat-inactivated human lymphocytes in mixed-lymphocyte culture.

    abstract::Possible causes were examined for the inability of heat-inactivated lymphocytes to induce proliferative responses in mixed-lymphocyte cultures (MLC). Cells heated at 45 degrees C for 60 min lost greater than 90% of their capacity to stimulate in primary (1 degree) or secondary (2 degrees) MLC. This was not due to acce...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Boyer CM,Kostyu DD,Brissette CS,Amos DB

    更新日期:1986-09-01 00:00:00

  • Role of the production of natural killer cell stimulatory factor (NKSF/IL-12) in the ability of B cell lines to stimulate T and NK cell proliferation.

    abstract::We have previously used human Epstein Barr virus (EBV)-transformed B lymphoblastoid cell lines for the identification and purification of a novel cytokine, natural killer cell stimulatory factor (NKSF/IL-12), that has pleiotropic effects on human lymphocytes. B cell lines are also routinely employed as feeder cells fo...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Valiante NM,Rengaraju M,Trinchieri G

    更新日期:1992-11-01 00:00:00

  • Inhibition of interleukin 2 production by prostaglandin E2 is not absolute but depends on the strength of the stimulating signal.

    abstract::In view of the eminently important role of interleukin-2 (IL-2) in T-cell responses, and in view of reports about immune stimulatory effects of PGE2, we reinvestigated the question whether PGE2 inhibits IL-2 production. It was found that PGE2 does not inhibit IL-2 production in murine spleen cell cultures after optima...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Wolf M,Falk W,Männel D,Dröge W

    更新日期:1985-01-01 00:00:00

  • Macrophage I-A/I-E expression and macrophage-stimulating lymphokines in murine lupus.

    abstract::Seeking common abnormalities in mice genetically predisposed to lupus-like autoimmune disease, we investigated (1) the ontogeny of Ia antigens (I-A/I-E) on the surfaces of resident peritoneal macrophages (rpM phi) of lupus and normal mice, (2) spontaneous and lectin-induced in vitro production of M phi-stimulating fac...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kofler R,Schreiber RD,Dixon FJ,Theofilopoulos AN

    更新日期:1984-08-01 00:00:00

  • Evaluation of metabolic and immunological changes in streptozotocin-nicotinamide induced diabetic rats.

    abstract::Type 2 diabetes is a chronic disease with growing public health concern globally. Finding remedies to assist this health issue requires recruiting appropriate animal model for experimental studies. This study was designated to evaluate metabolic and immunologic changes in streptozotocin-nicotinamide induced diabetic r...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Mojani MS,Sarmadi VH,Vellasamy S,Sandrasaigaran P,Rahmat A,Peng LS,Ramasamy R

    更新日期:2014-05-01 00:00:00

  • Divergent effects of cyclosporine A on interferon- and mitogen-induced Ly-6A antigen suggest autocrine regulation of Ly-6A expression in activated T cells.

    abstract::The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we com...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Dumont FJ

    更新日期:1987-04-01 00:00:00

  • Igh-6b expression in BALB.nude mice.

    abstract::Mice that differ at Igh loci and the reagents that permit resolution of such distinctions are powerful genetic tools for studying B cell heterogeneity. In this report, IgM-allotype-specific ELISA revealed that two of three sources of mice designated as BALB.nude express the Igh-6b (IgMb) allotype characteristic of C57...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Riggs JE

    更新日期:1993-05-01 00:00:00

  • Induction of T-lymphocyte adhesion by histidine-proline-rich glycoprotein and concanavalin A.

    abstract::Histidine-proline-rich glycoprotein (HPRG) is a plasma protein which binds to a specific receptor on T-lymphocytes and represses T-cell activation and proliferation. In the presence of Concanavalin A (Con A), HPRG causes human T-lymphoblastic MOLT-3 cells and a fraction of normal human peripheral blood lymphocytes to ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Lamb-Wharton RJ,Morgan WT

    更新日期:1993-12-01 00:00:00

  • Phenotypic differences between mice deficient in XIAP and SAP, two factors targeted in X-linked lymphoproliferative syndrome (XLP).

    abstract::Mutations in the X-linked inhibitor of apoptosis (XIAP) have recently been identified in patients with the rare genetic disease, X-linked lymphoproliferative syndrome (XLP), which was previously thought to be solely attributable to mutations in a distinct gene, SAP. To further understand the roles of these two factors...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Rumble JM,Oetjen KA,Stein PL,Schwartzberg PL,Moore BB,Duckett CS

    更新日期:2009-01-01 00:00:00

  • Impaired rearrangement of IgH V to DJ segments in bone marrow Pro-B cells from old mice.

    abstract::There are fewer bone marrow Pre-B cells in old compared to young mice. We have demonstrated both decreased rearrangement of the V to DJ IgH gene segments and low levels of VH germline transcripts in Pro-B cells, the precursors of Pre-B cells, from old compared to young mice. However, there was no difference in the lev...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Szabo P,Shen S,Telford W,Weksler ME

    更新日期:2003-03-01 00:00:00

  • A cell permeant peptide containing the cytoplasmic tail sequence of Fc receptor type IIA reduces calcium signaling and phagolysosome formation in neutrophils.

    abstract::Receptors for the Fc domain of IgG mediate target recognition, signal transduction, and effector functions including antibody-dependent cytolysis, phagocytosis, and phagolysosome formation. To better understand FcR-mediated functions and to identify potential therapeutic strategies, we employed cell-penetrating ("Troj...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Clark AJ,Petty HR

    更新日期:2010-01-01 00:00:00

  • Beta2-integrin, LFA-1, and TCR/CD3 synergistically induce tyrosine phosphorylation of focal adhesion kinase (pp125(FAK)) in PHA-activated T cells.

    abstract::Complete T cell activation requires not only a first signal via TCR/CD3 engagement but also a costimulatory signal through accessory receptors such as CD2, CD28, or integrins. Focal adhesion kinase, pp125(FAK) (FAK), was previously shown to be localized in focal adhesions in fibroblasts and to be involved in integrin-...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Tabassam FH,Umehara H,Huang JY,Gouda S,Kono T,Okazaki T,van Seventer JM,Domae N

    更新日期:1999-05-01 00:00:00

  • The effects of temperature and oleic acid on murine memory and virgin T cell activation: interleukin-2 secretion and interleukin-2 receptor expression.

    abstract::Previous studies established that low in vitro temperatures (27 degrees C, termed nonpermissive) suppressed murine primary thymus-dependent (TD), but not primary thymus-independent (TI) or secondary TD, antibody responses. Suppression was rescued by the addition of oleic acid (18:1), recombinant interleukin (rIL)-2 an...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Wooten RM,Clem LW,Bly JE

    更新日期:1993-11-01 00:00:00

  • Induction of hyporesponsiveness in human lymphocytes-T despite their expression of both the co-receptor CD28 and its ligand B7.

    abstract::A majority (42/62) of TCR2+ interleukin 2-dependent human T lymphocyte clones was found concordantly to express not only the CD28 co-receptor structure at the cell surface but also its ligand B7. Interactions between CD28 and B7 can have important consequences for T cell activation, particularly in providing "signal 2...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Friccius H,Siegels-Hübenthal P,Rehbein A,Schlotz E,Pawelec G

    更新日期:1993-10-15 00:00:00

  • Characterization of cadherins expressed by murine thymocytes.

    abstract::Thymocytes develop in close apposition to the stromal cells of the thymus. The ontogeny of thymocytes is dependent on intimate interactions between these cells and the stromal cells. The molecular mechanisms involved in regulating thymocyte-stromal cell interactions remain to be clearly defined. In this study, we util...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Munro SB,Duclos AJ,Jackson AR,Baines MG,Blaschuk OW

    更新日期:1996-05-01 00:00:00

  • Mechanisms in opposite modulation of spleen cell and lymph node cell responses to mitogens following muramyl dipeptide treatment in vivo.

    abstract::It has been reported that a muramyl dipeptide (MDP) treatment regimen (200 micrograms MDP per mouse, Days -4, -3, -2, and -1) that, given prophylactically, affords protection against several infectious agents also induces lymph node hyperplasia, lymph node cell (LNC) hyperresponsiveness to mitogens, and spleen cell hy...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Brummer E,Stevens DA

    更新日期:1985-04-01 00:00:00

  • Analysis of B-cell subpopulations. I. Relationships among splenic xid, Ly 1+, and Lyb 5+ B cells.

    abstract::The phenotypic and functional relationships among the various B-cell subsets is of importance for better understanding studies of the immune system. In this report, the realm of B cells encompassed by Lyb 5, Ly 1, and xid has been examined through the use of the alloantiserum anti-Lyb 5, the unique functional properti...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Smith HR,Yaffe LJ,Chused TM,Raveche ES,Klinman DM,Steinberg AD

    更新日期:1985-04-15 00:00:00

  • Leukocyte complement: interleukin-like properties of factor Bb.

    abstract::It has been previously shown that the activated form of Factor B (Factor Bb) of the alternative pathway of complement activation stimulates monocyte spreading and killing of xenogenic erythrocytes and staphylococci. Factor Bb also stimulates lymphocyte blastogenesis in vitro, and native (uncleaved) Factor B is a major...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Hirani S,Fair DS,Papin RA,Sundsmo JS

    更新日期:1985-05-01 00:00:00

  • Aging, sex, inflammation, frailty, and CMV and HIV infections.

    abstract::Aging is characterized by significant immune remodeling at both cellular and molecular levels, also known as immunosenescence. Older adults often manifest a chronic low-grade inflammatory phenotype. These age-related immune system changes have increasingly been recognized not only to lead to immune functional decline ...

    journal_title:Cellular immunology

    pub_type: 杂志文章,评审


    authors: Leng SX,Margolick JB

    更新日期:2020-02-01 00:00:00

  • Powerful immunosuppression mediated by interleukin 2-activated, nonantigen-specific, or H-2-restricted THY-1+ CD8+ cells.

    abstract::Normal spleen cells cultured in high or low concentrations of interleukin (IL) 2 for 3 days contain Thy-1+ CD4- CD8+ cells that powerfully suppress primary but not ongoing or active lymphocyte responses. The precursors of these cells are Thy-1+ AGM-1- and are absent or present in greatly diminished numbers in athymic ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Torre-Amione G,Tuetken R,Rowley DA

    更新日期:1989-11-01 00:00:00

  • Lymphocyte subsets involved in tumor-activated nonspecific feedback suppression.

    abstract::Cells from the spleen, lymph nodes, and peritoneum of DBA/2 mice bearing a subcutaneous tumor mediate nonspecific suppression of an in vitro antibody response to sheep red blood cells (SRBC) when cocultured with a normal T-cell subset(s). The spleen cells from the tumor-bearing mouse required for the suppression bear ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ingenito GG,Calkins CE

    更新日期:1983-07-01 00:00:00

  • Piper nigrum extract ameliorated allergic inflammation through inhibiting Th2/Th17 responses and mast cells activation.

    abstract::Piper nigrum (Piperaceae) is commonly used as a spice and traditional medicine in many countries. P. nigrum has been reported to have anti-oxidant, anti-bacterial, anti-tumor, anti-mutagenic, anti-diabetic, and anti-inflammatory properties. However, the effect of P. nigrum on allergic asthma has not been known. This s...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Bui TT,Piao CH,Song CH,Shin HS,Shon DH,Chai OH

    更新日期:2017-12-01 00:00:00