Abstract:
:Tumors harbor a population of cancer stem cells (CSCs) which can drive tumor progression and therapeutical resistance. Nature killer (NK) cells are best known for their ability to directly recognize and kill malignant cells. However, the susceptibility of cancer stem cells to NK cells is not fully understood. Here we demonstrated that human CD44+CD24- breast CSCs were shown enhanced sensitivity to IL-2 and IL-15 activated NK cells. CD44+CD24- CSCs expressed higher levels of NKG2D ligands ULBP1, ULBP2 and MICA. Blockade assay showed that the sensitivity of CSCs to NK cells-mediated lysis was mainly dependent on NKG2D. Furthermore, redox oxygen species (ROS)-low tumor cells were more sensitive to NK cells. The presence of antioxidant enzymes inhibitor L-S,R-buthionine sulfoximine or H2O2 retarded the cytotoxicity of NK cells to CD44+CD24- CSCs. In addition, NK cells could readily target CD133+ colonal CSCs. Our findings provide novel targets for NK cells-based immunotherapy and are of great importance for translational medicine.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Yin T,Wang G,He S,Liu Q,Sun J,Wang Ydoi
10.1016/j.cellimm.2015.11.009subject
Has Abstractpub_date
2016-02-01 00:00:00pages
41-5eissn
0008-8749issn
1090-2163pii
S0008-8749(15)30043-5journal_volume
300pub_type
杂志文章abstract::The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate th...
journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1997-08-25 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.01.002
更新日期:2006-10-01 00:00:00
abstract::Two interleukin-2 (IL-2)-dependent cytotoxic T-cell clones were obtained by limiting dilution from a lymphocyte culture stimulated in vitro with the autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) in the presence of fetal calf serum (FCS). Both clones uniformly had a T3+, T4+, Dr+ phenotype an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90304-7
更新日期:1986-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90003-2
更新日期:1988-04-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90180-7
更新日期:1985-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0123
更新日期:1996-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0071
更新日期:1996-03-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90158-9
更新日期:1986-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1058
更新日期:1993-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0232
更新日期:1996-09-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.103987
更新日期:2020-01-01 00:00:00
abstract::Short-term culture of murine lymphocytes in interleukin 2 (IL-2), in the absence of any priming antigen, has been shown to result in the differentiation of an activated killer cell population capable of potent cytotoxic activity against tumor cells. The progenitor and lineage of these lymphokine activated killer cells...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90089-4
更新日期:1986-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0208
更新日期:1996-08-25 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90400-9
更新日期:1985-06-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90275-3
更新日期:1986-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1059
更新日期:1994-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90278-y
更新日期:1990-10-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90156-5
更新日期:1986-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90033-9
更新日期:1984-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.04.017
更新日期:2005-03-01 00:00:00
abstract::It is becoming increasingly clear that regulatory T cells (Treg cells) in specific tissues are important parts of immune system. Tissue-resident Treg cells, which are largely Foxp3-expressing CD4(+) Treg cells, are distinct from one another and conventional Treg cells, and have tissue-specific phenotype and function. ...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
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更新日期:2015-09-01 00:00:00
abstract::Attempts have been made to induce cytolytic T cells to kill target cells that do not express the appropriate target molecules by crosslinking the T cells and the target cells in various ways. One successful strategy has been to use heteroconjugates or bispecific monoclonal antibodies reacting with T cell molecules wit...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90218-g
更新日期:1990-09-01 00:00:00
abstract::It is still a matter of debate, whether tolerance toward self-non-MHC antigens is due to intrathymic deletion or to regulatory processes in the periphery. To further pursue this question, responsiveness toward TNP and an anti-TNP monoclonal antibody (Sp6) carrying a recurrent idiotype was evaluated in prenatally trini...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90298-6
更新日期:1990-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.09.008
更新日期:2012-09-01 00:00:00
abstract::In this study the influence of a non-mitogenic anti-CD3 antibody on accessory cell-dependent antigen and mitogen-induced T-lymphocyte proliferation has been investigated. The antibody was found to completely inhibit PHA, Con A, PWM, and tetanus toxoid stimulation, with no effect on the proliferation induced by the cal...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90014-6
更新日期:1986-06-01 00:00:00
abstract::Peroxynitrite (ONOO(-)) has been vastly implicated in mutagenesis and cancer development. Present study probes the antigenicity of peroxynitrite damaged DNA (ONOO(-)-DNA) in cancer patients. Purified human placental DNA was damaged by the synergistic action of sodium nitroprusside (SNP) and Pyrogallol for 3h at 37 deg...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.08.002
更新日期:2009-01-01 00:00:00