Abstract：
:In the mouse, activation of T cells by T cell receptor (TCR) crosslinking with anti-CD3 antibodies in the absence of a costimulatory signal induces Th1 but not Th2 cell anergy. Furthermore, anti-CD3 induces anergy of Th1- but not Th2-type lymphokine secretion in Th0 cells. This study was designed to determine whether this is also the case in man. Human rye grass allergen Lol p I-specific cloned CD4+ T helper cells of subtypes Th0, Th1, and Th2 were treated with immobilized anti-CD3. The cells were rested for 4 days and then activated under optimal conditions with antigen and antigen-presenting cells (APCs). Cell proliferation and IL-2, IFN-gamma, and IL-4 secretion was determined to test for the anergic state. The initial anti-CD3 treatment induced cell proliferation, IL-2, IFN-gamma, and/or IL-4 secretion by T cells of all three subsets which was followed by an anergic state in Th0, Th1, and Th2 cells as shown by a 51 to > 94% decrease in cell proliferation and IFN-gamma and/or IL-4 secretion after subsequent APC and Lol p I activation. Addition of IL-2 or IL-4 during anti-CD3 treatment of the cells did not prevent unresponsiveness. However, the addition of IL-2 but not IL-4 during APC and Lol p I stimulation partially reversed the anergic state. These data demonstrate that, contrary to the mouse, cloned T cells of all three human T helper cell subtypes are anergized by anti-CD3 TCR activation in the absence of costimulatory signals. The fact that human Th2 cells can be anergized may be important for the development of new treatments in Th2-mediated allergic disorders.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Nguyen DD,Beck L,Spiegelberg HLdoi
10.1006/cimm.1995.1199subject
Has Abstractpub_date
1995-10-01 00:00:00pages
153-7issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(85)71199-9journal_volume
165pub_type
杂志文章abstract：:Previous studies have validated the capacity of classical (HLA-A,B,C) and nonclassical (HLA-G) MHC class I antigens expressed by target cells to influence natural killer (NK) cell-mediated cytolysis. Generally, elevated expression of these HLA class I molecules is correlated with enhanced resistance to lysis by NK cel...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0020
更新日期：1996-01-10 00:00:00
abstract：:T-Cell subsets identified by polyclonal and monoclonal antibodies to dipeptidyl peptidase IV (DP IV) were investigated. Analysis in a cytofluorograf revealed 63 +/- 7% positive scatter-gated T lymphocytes. DP IV-positive cells were found to be T11+, 74-81% OKT4+, and 12-19% OKT8+. DP IV-negative cells were T11+ and co...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90400-9
更新日期：1985-06-01 00:00:00
abstract：:With DNA vaccines, it is important to monitor the movement of transfectants and to overcome immune deviations. We used a pCMV-LacZ plasmid (expressing β-galactosidase) and a pcDNA-hNIS plasmid (expressing the human sodium/iodide symporter [hNIS] gene) as non-secreted visual-imaging markers. Transfectants carrying the ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.04.003
更新日期：2018-07-01 00:00:00
abstract：:Observational studies in multiple sclerosis (MS) demonstrated altered expression of chemokine receptors (CkRs) on comparable populations of mononuclear cells (e.g. CD4(+)/CD45RO(+) T-cells) in brain sections compared with blood. These findings raised questions about the regulation of CkRs on trafficking cells. Regulat...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.12.001
更新日期：2006-09-01 00:00:00
abstract：:Intravenous injection of spleen cells (SC) coated with an antitrinitrophenyl (anti-TNP) IgM monoclonal antibody, Sp6 (Sp6-SC), which carries a recurrent idiotype, resulted in activation of a Lyt-2-positive population which did adhere to Sp6-coated plates. No effect of Sp6-SC injection could be observed in vivo on an a...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90314-x
更新日期：1986-04-01 00:00:00
abstract：:Supplementation of the diet with fish oil (FO) decreases growth of the Walker 256 tumor and decreases the cachexia associated with tumor-bearing. The mechanisms by which FO inhibits tumor growth and cachexia are unknown. Macrophages are very important in host defence against tumors since they produce several anti-tumo...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2004.12.001
更新日期：2004-09-01 00:00:00
abstract：:The biochemical events leading to enhanced membrane expression of HLA-DR and CR3 by human peripheral blood monocytes (MO) following exposure to bacterial lipopolysaccharide (LPS) were examined. In a previous study we demonstrated that an increase in intracellular calcium was necessary, but not sufficient, for MO to in...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90209-7
更新日期：1987-08-01 00:00:00
abstract：:Burn injury induces immune dysfunction and alters numerous physiological parameters. While clinical studies indicate that burn injury size profoundly impacts patient immune status, only limited experimental studies have systematically addressed its impact on immune functional parameters. In the present study, mice wer...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/s0008-8749(03)00024-8
更新日期：2002-11-01 00:00:00
abstract：:The administration of low-density lipoproteins from chicken egg yolk (EyLDL) to mice induced T-dependent antibodies against bromelain-treated mouse erythrocytes (BrMRBC). The anti-BrMRBC antibodies produced antigen specifically by EyLDL appear to be identical to the antibodies produced polyclonally by lipopolysacchari...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90195-5
更新日期：1988-09-01 00:00:00
abstract：:The lymphokine IL-4 is a potent enhancer of anti-IgM-induced B cell proliferation. Although the mechanism of this enhancement is not known, a commonly held view suggests that IL-4 acts together with anti-IgM as a costimulating factor for the activation of a subpopulation of B cells. To evaluate this hypothesis we exam...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90327-8
更新日期：1991-04-15 00:00:00
abstract：:Large suppressive macrophages (LSM) were induced by restimulating spleen cells from rats with experimental autoimmune myasthenia gravis (EAMG) in vitro, with the autoantigen acetylcholine receptor (AChR) in the presence of cyclosporine A. LSM, purified from these cultures, are extremely potent suppressors of AChR-stim...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1445
更新日期：1999-04-10 00:00:00
abstract：:It has long been believed that mast cells play a crucial role in the development of many physiological changes during immediate allergic responses. This study was conducted to evaluate the anti-inflammation mechanism of Schizonepeta tenuifolia (ST) extract and ST purified chemicals on the PMA plus A23187-induced stimu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2012.05.012
更新日期：2012-05-01 00:00:00
abstract：:The proliferative response of peripheral blood mononuclear cells (PBMC) in synthetic serum-free media depends on the presence of sufficient amounts of transferrin (Tf). In the present communication we show that the reduction of Tf concentration in culture media results in a decreased proliferation, whereas lymphokine ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90038-d
更新日期：1991-02-01 00:00:00
abstract：:Activated macrophages are known to release a variety of immunoregulatory substances including the low-molecular-weight substances hydrogen peroxide and lactate. We report here that lactate but not hydrogen peroxide is capable of supporting a substantial production of T-cell growth factor (TCGF) in cultures of accessor...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90224-3
更新日期：1987-09-01 00:00:00
abstract：BACKGROUND:Piper nigrum L. (Piperaceae) is commonly used as a spice and traditional medicine in many countries. It has been reported to have anti-oxidant, anti-bacterial, anti-tumor, anti-mutagenic, anti-diabetic, and anti-inflammatory properties. However, the protective role of P. nigrum on epithelial function of uppe...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2019.104035
更新日期：2020-05-01 00:00:00
abstract：:Cytotoxic T-cell (CTL) cultures were generated from five ovarian cancer patients (OvCTL) and from three breast cancer patients (BrCTL). All CTL lines were T-cell receptor (TcR) alphabeta+ and predominantly CD8+ (73 +/- 13%). These CTL lines preferentially recognized autologous tumor cells in an HLA class I-restricted,...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.1055
更新日期：1997-02-01 00:00:00
abstract：:With old age (∼2y old), mice show substantial differences in B cell composition within the lymphoid tissues. In particular, a novel subset of IgM+ CD21/35lo/- CD23- mature B cells, the age-associated B cells or ABC, increases numerically and proportionately. This occurs at the expense of other B cell subsets, includin...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2017.04.008
更新日期：2017-11-01 00:00:00
abstract：:The engulfment of apoptotic cells by monocytes and unprimed macrophages results in M2 polarization. In the current study, we investigated whether apoptotic cells influence the phenotypic and functional characteristics of GM-CSF-differentiated human macrophages (GM-Mφ). Our results demonstrate that GM-Mφ preincubated w...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.06.002
更新日期：2018-09-01 00:00:00
abstract：:Within lymphopenic recipients, naïve T cells undergo proliferation that is induced by homeostatic mechanisms. Earlier studies have demonstrated that commensal antigens play a key role in inducing the proliferation. However, a relative contribution of endogenous self antigens in this process has not been formally inves...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.11.002
更新日期：2012-01-01 00:00:00
abstract：:The antifungal activity of nonactivated resident murine peritoneal macrophages for Cryptococcus neoformans was studied. Macrophages from five of six mouse strains tested had significant (40 to 80%) fungistatic activity, depending on the inoculum size, in a 24-hr coculture system. Macrophages from two outbred (SW and I...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1200
更新日期：1994-08-01 00:00:00
abstract：:We have performed immunoperoxidase staining on cryostat tissue sections and immunofluorescence analysis on cell suspensions to identify cells expressing the alpha/beta T cell antigen receptor during ontogeny and adult life in chickens. We used the mouse monoclonal antibody, TCR2, which was previously shown to recogniz...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90079-7
更新日期：1990-01-01 00:00:00
abstract：:Nontypeable Haemophilus influenzae (NTHi) is the etiological agent most frequently associated with bacterial exacerbations of chronic obstructive pulmonary disease (COPD). The present work shows that NTHi strains induced in primary normal human bronchial epithelial cells (NHBE) a cytokine/chemokine response in which C...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.09.008
更新日期：2010-01-01 00:00:00
abstract：:Mice from strains showing either phenotypical expression of Bcg gene (C57BL/6, BALB/c, DBA/1, and (C57BL/6 X DBA/2)/F1, CBA, A/J, DBA/2) were infected intravenously with 10(7) Mycobacterium lepraemurium (MLM). The number of acid-fast bacilli (AFB) within the spleens, the ability of spleen cells to produce in vitro int...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90026-7
更新日期：1985-03-01 00:00:00
abstract：:During normal lymphocyte recirculation and in chronic inflammation, lymphocytes emigrate from blood into the perivascular tissue. The mechanism of lymphocyte migration through the endothelial cell (EC) layer of blood vessels is poorly understood. To identify factors that control lymphocyte emigration, a method has bee...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90324-3
更新日期：1988-07-01 00:00:00
abstract：:Five thiol compounds including 2-mercaptoethanol (2-ME) were examined for their augmenting effects on in vitro antibody response to sheep erythrocytes. Three compounds were effective with the following order of activity; 2-ME greater than dithiothreitol greater than cysteamine. Glutathione and thioglycollate failed to...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90060-6
更新日期：1983-07-01 00:00:00
abstract：:Intravital imaging of cutaneous immune responses has revealed intricate links between the skin's structural properties, the immune cells that reside therein, and the carefully orchestrated migratory dynamics that enable rapid sensing and subsequent elimination of skin pathogens. In particular, the development of 2-pho...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2019.04.001
更新日期：2020-04-01 00:00:00
abstract：BACKGROUND:Interleukin (IL)-23, composed of p19 and p40 subunits, has diverse functions in regulating immune systems, enhancing cell-mediated immunity. In the present study, we investigated whether forced expression of the p19-linked p40 gene in murine mammary cancer cells (MA891) produced antitumor effects in vivo. Tu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.05.002
更新日期：2009-01-01 00:00:00
abstract：:Experimental autoimmune neuritis (EAN) is a T-cell-mediated autoimmune disease characterized by demyelination and mononuclear cell infiltration of the peripheral nervous system. It is induced in Lewis rats by administration of myelin P2 protein or a synthetic peptide (SP-26) corresponding to amino acid residues 53-78 ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1156
更新日期：1994-06-01 00:00:00
abstract：:Immunization with synthetic mRNA encoding tumor-associated antigens is an emerging vaccine strategy for the treatment of cancer. In order to prevent mRNA degradation, promote antigen-presenting cells antigen presentation, and induce an anti-tumor immune response, we investigated the nasal administration of mRNA vaccin...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2020.104143
更新日期：2020-08-01 00:00:00
abstract：:Vaccination can elicit CD8(+) cytotoxic T lymphocytes (CTL) that recognize peptides presented by class I MHC molecules. Relatively little is known, however, about the genetic factors that shape the repertoire of T cell clonotypes responding to any given epitope. We report here that H-2(b) mice immunized with a plasmid...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1338
更新日期：1998-08-25 00:00:00
