Notch signaling regulates CXCR4 expression and the migration of mesenchymal stem cells.


:Mesenchymal stem cells (MSCs) have been used to repair injured tissues through immune-suppression and/or cell replace mechanisms. However, a significant barrier to MSC therapy is insufficient MSC engraftment in injured tissues after systemic administration. Here, we report that cell surface, total protein, and mRNA levels of CXCR4 were significantly increased in MSCs when Notch signaling was interrupted by γ-secretase inhibitor (GSI) or knockout of the transcription factor RBP-J, which mediates signaling from all four mammalian Notch receptors. The GSI-treated or RBP-J deficient MSCs showed stronger migration toward stromal cell-derived factor-1α (SDF-1α) than that of the control. In a mouse hepatic ischemia/reperfusion model, RBP-J deficient MSCs migrated into the injured liver tissues at a significantly higher efficiency than that of the control MSCs. Mice transfused with RBP-J deficient MSCs showed reduced liver damage. Therefore, Notch signaling regulates MSC migration and function, at least partially via the modulation of CXCR4 expression.


Cell Immunol


Cellular immunology


Xie J,Wang W,Si JW,Miao XY,Li JC,Wang YC,Wang ZR,Ma J,Zhao XC,Li Z,Yi H,Han H




Has Abstract


2013-01-01 00:00:00














  • Generation of lymphokine-activated killer (LAK) cells from tumor-infiltrating lymphocytes.

    abstract::Culture of tumor-infiltrating lymphocytes (TIL) containing about 20% BMC2 tumor cells with recombinant human interleukin 2 (rIL-2) resulted in the diminish of tumor cells and the growth of lymphocytes. These IL-2-activated lymphocytes showed a strong cytotoxic activity against not only syngeneic tumor cells but also a...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Nishimura T,Yagi H,Uchiyama Y,Hashimoto Y

    更新日期:1986-06-01 00:00:00

  • The conventional CD4+ T cell response to staphylococcal enterotoxin B is modified by its superantigenic activity.

    abstract::Because of the massive cytokine response elicited by superantigen exposure, it has been suggested that superantigens may act as adjuvants to boost conventional antigen responses. However, most previous studies have shown that in vivo exposure to superantigen suppressed subsequent T cell responses. Here we analyzed the...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Wen R,Surman S,Blackman MA,Woodland DL

    更新日期:1997-03-15 00:00:00

  • Influence of immunomodulatory drugs on the cytotoxicity induced by monoclonal antibody 17-1A and interleukin-2.

    abstract::Patients treated with monoclonal antibodies and cytokines for cancer receive often co-medication, which may influence treatment efficacy. Therefore, we investigated with a flowcytometric cytotoxicity assay the effect of several immunomodulatory drugs on antibody dependent cellular cytotoxicity (ADCC), interleukin-2 (I...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Flieger D,Varvenne M,Kleinschmidt R,Schmidt-Wolf IG

    更新日期:2007-03-01 00:00:00

  • Tumor necrosis factor-alpha analyzed within individual macrophages by combined immunocytochemistry and computer-aided image analysis.

    abstract::Immunocytochemical staining procedures were combined with computer-aided image analysis to quantitate the relative intracellular production of tumor necrosis factor-alpha (TNF) within individual macrophages. Optimal conditions for time and methods for the activation of TNF production, fixation of cells for optimal imm...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Brown AR,Fishman M

    更新日期:1990-10-15 00:00:00

  • Thymic alterations in Plasmodium berghei-infected mice.

    abstract::The primary function of the thymus is to develop immature T-cells into cells that further in the periphery will be able to carry out immune functions. The Literature has shown that thymus can be a target for many pathogens and severe structural alterations take place in this organ during infectious diseases. Here, we ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Andrade CF,Gameiro J,Nagib PR,Carvalho BO,Talaisys RL,Costa FT,Verinaud L

    更新日期:2008-05-01 00:00:00

  • Key genes and proteins involved in CTCM-reducing microvascular endothelial cell permeability induced by SLT-IIv using gene chips and DIGE.

    abstract::An Affymetrix mouse genome array and differential in-gel electrophoresis (DIGE) techniques were used to investigate the pharmacological mechanisms of a mixture of herbs, designated CTCM, a compound of traditional Chinese medicine, for the treatment of increased permeability in mouse intestinal microvascular endothelia...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Yi P,Guo Y,Wang X,Mu X,Wei X

    更新日期:2010-01-01 00:00:00

  • Studies on the relationship of human natural killer and lymphokine-activated killer cells with lysosomal staining and analysis of surface marker phenotypes.

    abstract::We have previously demonstrated that natural killer (NK) cells are lysosome-rich and stain more intensely with lysosomotropic agents such as neutral red and quinacrine (Qu) than do non-NK cells. In this study we combined the quantitation of Qu staining with surface marker staining to define subpopulations of NK cells....

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Shau H,Gray D,Mitchell MS

    更新日期:1988-08-01 00:00:00

  • Characterization of IL-2-induced murine cells which exhibit ADCC activity.

    abstract::The incubation of murine splenocytes in recombinant interleukin 2 (IL-2) gives rise to both lymphokine-activated killer (LAK) cells capable of lysing fresh tumor cells and cells capable of mediating antibody-dependent cellular cytotoxicity (ADCC) in the presence of anti-H2 allosera. A similarity between these two IL-2...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Eisenthal A,Shiloni E,Rosenberg SA

    更新日期:1988-09-01 00:00:00

  • Implications of a rise in cytosolic free calcium in the activation of RAW-264 macrophages for tumor cell killing.

    abstract::The concentration of cytosolic-free calcium (Ca2+i) was determined with aequorin in RAW-264 macrophage-like cells activated in vitro for tumor cell killing with lymphokine (LK) and lipopolysaccharide (LPS). Treatments of these cells with optimal doses of stimulants, which evoked the development of cytolytic activity, ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Gorecka-Tisera AM,Snowdowne KW,Borle AB

    更新日期:1986-07-01 00:00:00

  • Proliferative effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) and calcium ionophores on human large granular lymphocytes (LGL).

    abstract::The proliferative responses of natural killer (NK) cells to 12-O-tetradecanoylphorbol-13-acetate (TPA), which directly activates protein kinase c(PKC), and to the Ca2+ ionophores A23817 and ionomycin, known to enhance the intracellular calcium, have been investigated. Highly purified large granular lymphocytes (LGL) w...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Procopio A,Gismondi A,Paolini R,Morrone S,Testi R,Piccoli M,Frati L,Herberman RB,Santoni A

    更新日期:1988-04-15 00:00:00

  • Treatment of murine gliomas by adoptive transfer of ex vivo activated tumor-draining lymph node cells.

    abstract::The adoptive transfer of tumor-reactive T lymphocytes has recently been demonstrated to be an effective means for mediating the regression of experimental intracranial fibrosarcomas. In this study, mice bearing syngeneic intracranial GL261 gliomas were cured by the combination of sublethal whole body irradiation follo...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Plautz GE,Touhalisky JE,Shu S

    更新日期:1997-06-15 00:00:00

  • Activin A down-regulates the phagocytosis of lipopolysaccharide-activated mouse peritoneal macrophages in vitro and in vivo.

    abstract::Activin A is a multifunctional factor of the transforming growth factor-beta (TGF-beta) superfamily and acts as an anti-inflammatory cytokine produced by microglia and macrophages. In this study, we investigated the regulatory effect and possible mechanism of activin A on activation of lipopolysaccharide (LPS)-induced...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Zhou J,Tai G,Liu H,Ge J,Feng Y,Chen F,Yu F,Liu Z

    更新日期:2009-01-01 00:00:00

  • The CR2 receptor (CD21) shows increased expression in the more differentiated cells of an antigen-specific B cell line.

    abstract::The complement receptor 2 (CR2; CD21), a 145,000 MW glycoprotein, has been useful as a marker of B lymphocyte maturation. It is expressed on the 1:13 monoclonal, EBV-transformed, B cell line which produces TNP-specific IgM-kappa and displays an in vitro capacity for differentiation. The line expresses the CD20+CD21+ p...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Pillemer SR,Tsokos GC,Barbieri SA,Balow JE,Golding B

    更新日期:1990-02-01 00:00:00

  • Characterization of human leucocytes bearing the IL-3 receptor.

    abstract::Human leucocytes from peripheral blood and tonsil were examined for the presence of the IL-3 receptor using monoclonal antibodies directed to epitopes of the alpha and beta chains of the receptor. We found that the beta chain, common to IL-3, IL-5, and GM-CSF, was either present at low levels or not detected on the ma...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Macardle PJ,Chen Z,Shih CY,Huang CM,Weedon H,Sun Q,Lopez AF,Zola H

    更新日期:1996-02-25 00:00:00

  • Arginine analogues suppress antigen-specific and -nonspecific T lymphocyte proliferation.

    abstract::Using analogues of arginine to inhibit nitric oxide (NO.) production, investigators have demonstrated the intermediary role of NO. in a variety of physiological events including the antimicrobial activity exhibited by macrophages in vitro. In an effort to establish the effector function of NO. in the antimicrobial act...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Gregory SH,Sagnimeni AJ,Wing EJ

    更新日期:1994-02-01 00:00:00

  • Disruption of membrane cholesterol stimulates MyD88-dependent NF-kappaB activation in immature B cells.

    abstract::Agents that extract or sequester membrane cholesterol stimulate IkappaB degradation and lead to NF-kappaB activation in a subset of B cells. Although the extraction of cholesterol by methyl-beta-cyclodextrin is the most potent stimulus of NF-kappaB, other agents that sequester cholesterol have similar effects. B cells...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Flemming JA,Perkins KH,Luus L,Ferguson AR,Corley RB

    更新日期:2004-05-01 00:00:00

  • Alterations in structure and cellular localization of molecular forms of DP IV/CD26 during T cell activation.

    abstract::Dipeptidyl peptidase IV (DP IV, CD26), known as an activation marker of T lymphocytes, is a proline-specific protease thought to be involved in the regulation of the immune response. The physiological role of dipeptidyl peptidase IV in the immune system and the molecular events of lymphocyte activation mediated by thi...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kähne T,Kröning H,Thiel U,Ulmer AJ,Flad HD,Ansorge S

    更新日期:1996-05-25 00:00:00

  • Mechanism of augmentation of the antibody response in vitro by 2-mercaptoethanol in murine lymphocytes. II. A major role of the mixed disulfide between 2-mercaptoethanol and cysteine.

    abstract::Five thiol compounds including 2-mercaptoethanol (2-ME) were examined for their augmenting effects on in vitro antibody response to sheep erythrocytes. Three compounds were effective with the following order of activity; 2-ME greater than dithiothreitol greater than cysteamine. Glutathione and thioglycollate failed to...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ohmori H,Yamamoto I

    更新日期:1983-07-01 00:00:00

  • Transduction of human melanoma cells with interleukin-2 gene reduces tumorigenicity and enhances host antitumor immunity: a nude mouse model.

    abstract::Human melanoma tumor cells were genetically modified in vitro by transferring the interleukin-2 (IL-2) gene via a retroviral vector into established or fresh tumor cells. In addition, human melanoma cells were transduced in vivo by the direct injection of the IL-2/retroviral vector into melanoma xenografts in nude mic...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Abdel-Wahab Z,Li WP,Osanto S,Darrow TL,Hessling J,Vervaert CE,Burrascano M,Barber J,Seigler HF

    更新日期:1994-11-01 00:00:00

  • Cytostatic and cytolytic activities of macrophages regulation by prostaglandins.

    abstract::Murine peritoneal macrophages (M phi), activated in vivo or in vitro, remarkably inhibited the uptake of thymidine by a lens epithelial cell line, while resident M phi, or M phi induced by thioglycollate, exhibited much lower or no cytostatic capacity. The target cells were partially protected from the cytostatic acti...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Mochizuki M,Zigler JS Jr,Russell P,Gery I

    更新日期:1984-01-01 00:00:00

  • Distinct immunologic specificity of tumor regression mediated by effector cells isolated from immunized and tumor-bearing mice.

    abstract::Sensitized T lymphocytes can mediate potent antitumor effects when transferred to tumor-bearing animals. Employing the MCA 105 and MCA 106 sarcomas, we were able to generate antitumor effector cells by immunization of syngeneic mice with tumor cells admixed with Corynebacterium parvum. These immune splenocytes could b...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Chang AE,Perry-Lalley DM,Shu S

    更新日期:1989-05-01 00:00:00

  • A role for L3T4+ T cells and their lymphokines in the generation of suppressor effector (TS3) cells.

    abstract::The cellular requirements for the in vitro induction of antigen-specific suppressor T cells were examined. Previous reports indicated that Ia-bearing macrophages and anti-idiotypic B cells are required as accessory cells to facilitate the generation of suppressor effector (TS3) cells which regulate the response to the...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: O'Hara RM Jr,Hausman PB,Sherr DH,Abromson-Leeman SR,Dorf ME

    更新日期:1988-10-15 00:00:00

  • Anti-CD3-induced anergy in cloned human Th0, Th1, and Th2 cells.

    abstract::In the mouse, activation of T cells by T cell receptor (TCR) crosslinking with anti-CD3 antibodies in the absence of a costimulatory signal induces Th1 but not Th2 cell anergy. Furthermore, anti-CD3 induces anergy of Th1- but not Th2-type lymphokine secretion in Th0 cells. This study was designed to determine whether ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Nguyen DD,Beck L,Spiegelberg HL

    更新日期:1995-10-01 00:00:00

  • Down modulation of human TLR3 function by a monoclonal antibody.

    abstract::Toll-like receptors are a family of pattern-recognition receptors that contribute to the innate immune response. Toll-like receptor 3 (TLR3) signals in response to foreign, endogenous and synthetic ligands including viral dsRNA, bacterial RNA, mitochondrial RNA, endogenous necrotic cell mRNA and the synthetic dsRNA an...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Duffy KE,Lamb RJ,San Mateo LR,Jordan JL,Canziani G,Brigham-Burke M,Korteweg J,Cunningham M,Beck HS,Carton J,Giles-Komar J,Duchala C,Sarisky RT,Mbow ML

    更新日期:2007-08-01 00:00:00

  • In situ analysis of T cell subset composition in experimental autoimmune thyroiditis after adoptive transfer of activated spleen cells.

    abstract::T cells from genetically susceptible mice developing experimental autoimmune thyroiditis (EAT) proliferate in response to restimulation with mouse thyroglobulin (MTg) in vitro. The in vitro-activated cells adoptively transfer EAT as well as differentiate into cells cytotoxic for syngeneic thyroid monolayers. To examin...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Conaway DH,Giraldo AA,David CS,Kong YC

    更新日期:1990-01-01 00:00:00

  • Native soluble CD5 delivers a costimulatory signal to resting human B lymphocytes.

    abstract::Recently, we reported that the CD5 protein can bind to the B cell antigen CD72. So far, no functional evidence has been given for this interaction. We used soluble native CD5 and two anti-CD72 monoclonal antibodies, JT3 and WL225, produced and characterized in our laboratory in order to investigate the role of CD5 in ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Van de Velde H,Thielemans K

    更新日期:1996-08-25 00:00:00

  • Effect of the antimicrobial peptide LL-37 on Toll-like receptors 2-, 3- and 4-triggered expression of IL-6, IL-8 and CXCL10 in human gingival fibroblasts.

    abstract::The antimicrobial peptide LL-37 is known to have a potent LPS-neutralizing activity in monocytes and macrophages. Recently, LL-37 in gingival crevicular fluids is suggested to be the major protective factor preventing infection of periodontogenic pathogens. In this study, we tried to address the effect of LL-37 on pro...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Into T,Inomata M,Shibata K,Murakami Y

    更新日期:2010-01-01 00:00:00

  • Degradation signals in ErbB-2 dictate proteasomal processing and immunogenicity and resist protection by cis glycine-alanine repeat.

    abstract::ErbB-2 is ubiquitinated and degraded when dissociated from its membrane chaperone or bound by specific antibody. Reagents which induce such degradation have demonstrated antitumor activity and may impact ErbB-2 immunogenicity. To further understand ErbB-2 degradation and immunogenicity, a glycine-alanine repeat (GAr) ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Piechocki MP,Pilon SA,Kelly C,Wei WZ

    更新日期:2001-09-15 00:00:00

  • Immature T lymphocytes in human cord blood identified by monoclonal antibodies: a model for the study of the differentiation pathway of T cells in humans.

    abstract::The reactivity of human cord blood lymphocytes was assessed against a panel of monoclonal antibodies (MoAb). The mean proportion of OKT3+ cells (pan-T) was significantly lower in cord blood (52 +/- 13.8%; mean +/- SD) compared with that of adult blood (75 +/- 8.9%) and paralleled well with the E-rosette-forming capaci...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Foa R,Giubellino MC,Fierro MT,Lusso P,Ferrando ML

    更新日期:1984-11-01 00:00:00

  • Surface mu heavy chain expressed on pre-B lymphomas transduces Ca2+ signals but fails to cause growth arrest of pre-B lymphomas.

    abstract::Little is known about the role of signals transduced by cell surface IgM (sIgM) expressed during early B cell development. A subclone (1.6) of the late pre-B cell lymphoma 70Z/3.12 was used to study signal transduction by surface mu heavy (H) chain before and after transition to the early immature B cell stage, and th...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Tsutsumi A,Terajima J,Jung W,Ransom J

    更新日期:1992-01-01 00:00:00