Abstract:
:Natural suppressor (NS) cells, which nonspecifically suppress immune responses, are present in the spleen following exposure to radiation, chronic graft-versus-host disease, or cancer and in normal bone marrow. A model system is described which allows the study of cytokines activating and inhibiting NS cells, cytokines mediating NS activity, and NS effects on cytokine synthesis. Recombinant interleukin-3 (rIL-3) and granulocyte-macrophage colony-stimulating factor (rGM-CSF) efficiently activated NS cells present in normal bone marrow and were effective at concentrations as low as 5 U/ml. At high concentrations, GM-CSF, but not IL-3, did not activate NS cells. Recombinant interferon-gamma (rIFN-gamma) blocked the activation of bone marrow NS cells by rIL-3, but did not down-regulate NS cells once activated. The NS cells secreted one or more soluble suppressor factors, which blocked IL-2 synthesis and also inhibited IL-2-dependent T cell proliferation in the presence of excess IL-2.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Moore SC,Theus SA,Barnett JB,Soderberg LSdoi
10.1016/0008-8749(92)90158-lsubject
Has Abstractpub_date
1992-05-01 00:00:00pages
398-408issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(92)90158-Ljournal_volume
141pub_type
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