Abstract：
:The role of protein synthesis during the activation of macrophages (M phi) by lymphokines (LK) was studied. Peritoneal murine macrophages elicited by proteose-peptone (pM phi) were activated with LK (supernatants from normal mouse spleen cells pulsed with concanavalin A) and tested for cytotoxicity in an 18 hr assay against 111In-labeled L5178Y lymphoma target cells. Reversible (cycloheximide and puromycin) or poorly reversible (emetine and pactamycin) inhibitors of protein synthesis were added during activation, and their effects on pM phi-mediated cytotoxicity and pM phi protein synthesis were measured. Minimal concentrations of inhibitors, reducing the rate of protein synthesis by more than 90% without toxic effects on macrophages, were chosen. Exposure of pM phi to LK for 2 to 18 hr in the presence of reversible inhibitors of protein synthesis did not affect the induction of cytolytic activity, indicating that protein synthesis was not required during the activation period. In contrast, activation of macrophages for 2 hr in the presence of poorly reversible inhibitors of protein synthesis resulted in a considerable reduction of cytolytic activity. The impairment of cytotoxic activity was also evident when pM phi were treated with such drugs during the first 2 hr of an 18 hr exposure to LK or when LK-activated macrophages were treated for 2 hr with the drugs before the addition of the targets. These results demonstrate that active protein synthesis is not required during the exposure of pM phi to LK, but that new proteins have to be synthesized to allow the expression of the cytotoxic activity in LK-activated pM phi.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Blasi E,Varesio Ldoi
10.1016/0008-8749(84)90273-9subject
Has Abstractpub_date
1984-04-15 00:00:00pages
15-24issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(84)90273-9journal_volume
85pub_type
杂志文章abstract：:7 anterior gradient homolog 2 (AGR2)-inhibited different molecular mutual positive correlation network was constructed in lung adenocarcinoma compared with human normal adjacent tissues by 17 overlapping molecules of 358 GRNInfer and 19 Pearson (AGR2 CC⩽-0.25). Based on GO, KEGG, GenMAPP, BioCarta and disease database...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2014.05.008
更新日期：2014-07-01 00:00:00
abstract：:Mycobacterium bovis BCG and its subcellular components (bacterial extract, culture filtrate, purified protein derivative, and muramyl dipeptide MDP) are potent in vitro IL-6 inducers in spleen cell cultures from uninfected and BCG-infected BALB/c mice. Both plastic adherent and nonadherent spleen cells are capable of ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90071-i
更新日期：1991-10-01 00:00:00
abstract：:The internalization of surface immunoglobulin (Ig) by B lymphocytes is the first step in the antigen-presenting function performed by these cells. Mature B cells coexpress on their surface IgM and IgD. At this time, there is controversy over whether these two isotypes serve different functions in the antigen-presentin...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90305-5
更新日期：1989-10-15 00:00:00
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journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90243-6
更新日期：1985-04-01 00:00:00
abstract：:Estrogen displays potent immunoregulatory properties. In the present study we investigated the in vivo effect of estrogen on natural killer (NK) cell cytotoxic activity in several mouse strains. After castration, 17 beta-estradiol was administered in low or high doses. After 3-6 weeks of hormone administration an in v...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1262
更新日期：1994-10-01 00:00:00
abstract：:The proliferative responses of natural killer (NK) cells to 12-O-tetradecanoylphorbol-13-acetate (TPA), which directly activates protein kinase c(PKC), and to the Ca2+ ionophores A23817 and ionomycin, known to enhance the intracellular calcium, have been investigated. Highly purified large granular lymphocytes (LGL) w...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90007-x
更新日期：1988-04-15 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSCs) play a major role in modulating immune response, but only a few reports focused on MDSCs in the liver of sepsis states. Here, we investigated the changes in MDSCs in liver of the cecal ligation and puncture (CLP) mice. The results of flow cytometry showed that MDSCs accumulate ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2012.08.005
更新日期：2012-09-01 00:00:00
abstract：:Two interleukin-2 (IL-2)-dependent cytotoxic T-cell clones were obtained by limiting dilution from a lymphocyte culture stimulated in vitro with the autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) in the presence of fetal calf serum (FCS). Both clones uniformly had a T3+, T4+, Dr+ phenotype an...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90304-7
更新日期：1986-04-01 00:00:00
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journal_title：Cellular immunology
pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90289-3
更新日期：1986-04-01 00:00:00
abstract：:The third complementarity determining region (CDR3) is the most variable part of alpha beta T cell receptors (TCR) and represents the putative antigen contacting site. To identify parameters determining the structural diversity of the CDR3 region, the CDR3 length distributions of 66 BV-J combinations in peripheral CD4...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0071
更新日期：1996-03-15 00:00:00
abstract：:Prevention of ultraviolet radiation- or chemical carcinogen-induced morphologic transformation and inhibition of tumor-producing transformed cell growth by lymphotoxin and by normal spleen leukocytes were quantitatively compared to define the antineoplastic activity spectra of these natural immune mediators. When Syri...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90372-6
更新日期：1983-03-01 00:00:00
abstract：:A fibroblastoid cell line TSt-4 was established from fetal thymus tissue of C57BL/6 mice. When fetal thymus (FT) cells or CD4-8- (DN) cells of adult thymuses were cultured on the monolayer of TSt-4, a considerable proportion of lymphocytes expressed CD4 or both CD4 and CD8 within 1 day, and the CD4+CD8- cells were mai...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90299-5
更新日期：1992-07-01 00:00:00
abstract：:A series of Lyt 2+, trinitrophenyl (TNP)-specific T-cell lines are shown to lyse 51Cr-labeled target cells in an antigen-specific, H-2-restricted fashion in a 4-hr assay. These cells also produce lymphotoxin, in addition to other factors, upon stimulation with TNP-haptenated syngeneic splenocytes. A technique for intr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90389-2
更新日期：1985-06-01 00:00:00
abstract：:Peripheral blood lymphocytes (PBL) isolated from BALB/c mice bearing a B-cell leukemia (BCL1) showed a marked proliferative response upon two days culturing with poly(L-lysine) (PLL) of various molecular weights. An inverse relationship was noted between the molecular weight of the PLL and the dose required for optima...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90203-5
更新日期：1985-02-01 00:00:00
abstract：:Lung cancer cells harboring multiple mutations as a consequence of long-term damage by different etiologic factors are responsible for high immunogenicity. Immune checkpoint inhibitors significantly improve treatment results in non-small cell lung cancer (NSCLC). Unfortunately, the role of T-lymphocytes in early NSCLC...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2017.09.007
更新日期：2017-12-01 00:00:00
abstract：:The mechanism underlying the apparent differences in the capacity of murine and human class I MHC molecules to function as signal transducing structures in T cells was examined. Cross-linking murine class I MHC molecules on splenic T cells did not stimulate an increase in intracellular calcium ([Ca2+]i) and failed to ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90254-m
更新日期：1992-10-15 00:00:00
abstract：:In vitro ultraviolet-B (UVB) irradiation of murine and rodent bone marrow cells prevents GVHD without compromising engraftment while inducing tolerance to donor-type allografts. In anticipation of clinical trials of UVB-modified bone marrow grafts, we studied the in vitro effects of UVB irradiation (50-300 J/m2) on hu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1202
更新日期：1995-10-15 00:00:00
abstract：:It is still a matter of debate, whether tolerance toward self-non-MHC antigens is due to intrathymic deletion or to regulatory processes in the periphery. To further pursue this question, responsiveness toward TNP and an anti-TNP monoclonal antibody (Sp6) carrying a recurrent idiotype was evaluated in prenatally trini...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90298-6
更新日期：1990-03-01 00:00:00
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journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.04.013
更新日期：2013-04-01 00:00:00
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journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1181
更新日期：1997-09-15 00:00:00
abstract：:Casitas B lymphoma-b (Cbl-b), a RING finger E3 ubiquitin ligase, has been identified as a critical regulator of adaptive immune responses. Cbl-b is essential for establishing the threshold for T cell activation and regulating peripheral T cell tolerance through various mechanisms. Intriguingly, recent studies indicate...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2018.11.002
更新日期：2019-06-01 00:00:00
abstract：:Mice that differ at Igh loci and the reagents that permit resolution of such distinctions are powerful genetic tools for studying B cell heterogeneity. In this report, IgM-allotype-specific ELISA revealed that two of three sources of mice designated as BALB.nude express the Igh-6b (IgMb) allotype characteristic of C57...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1125
更新日期：1993-05-01 00:00:00
abstract：:The mechanism of polyclonal B cell activation in autoimmune diseases was investigated by using an autoreactive B cell clone established by somatic hybridization with B cells derived from NZB X NZW (B/W) F1 mice. Briefly, splenic B cells from B/W F1 mice were fused with M12.4.1, a mutant of a B cell line, in the presen...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90080-9
更新日期：1992-02-01 00:00:00
abstract：:Virus-immune spleen cells induce fatal immunopathology following adoptive transfer into adult C57B1/6J mice that have been infected with lymphocytic choriomeningitis virus (LCMV) and immunosuppressed with cyclophosphamide. This is accompanied by the development of potent cytotoxic T-lymphocyte (CTL) activity of donor ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90260-7
更新日期：1987-06-01 00:00:00
abstract：:Previously, we reported that human B lymphocytes can be stimulated by trinitrophenylated Brucella abortus (TNP-Ba) in vitro to generate T-independent (TI) hapten specific plaque-forming cells (PFC). Furthermore we showed that addition of Con A on Day 3 of culture enhanced the anti-TNP response. In this report the char...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90245-4
更新日期：1984-05-01 00:00:00
abstract：:The antimicrobial peptide LL-37 is known to have a potent LPS-neutralizing activity in monocytes and macrophages. Recently, LL-37 in gingival crevicular fluids is suggested to be the major protective factor preventing infection of periodontogenic pathogens. In this study, we tried to address the effect of LL-37 on pro...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.05.005
更新日期：2010-01-01 00:00:00
abstract：:Germ-free (GF) mice have increased bone mass that is normalized by colonization with gut microbiota (GM) from conventionally raised (CONV-R) mice. To determine if innate immune signaling pathways mediated the effect of the GM, we studied the skeleton of GF and CONV-R mice with targeted inactivation of MYD88, NOD1 or N...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2017.05.003
更新日期：2017-07-01 00:00:00
abstract：:The long-term exposure of mice to superantigen SEA using a mini-osmotic pump (SEA pump) induced a long-lasting expansion of Vbeta3+ CD4+ T cells with T helper (Th) 2 cell-type properties. Removal of the SEA pump 10 days after pump implantation did not significantly alter the level of Vbeta3+ CD4+ T cell expansion/main...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.09.002
更新日期：2007-08-01 00:00:00
abstract：:The concentration of cytosolic-free calcium (Ca2+i) was determined with aequorin in RAW-264 macrophage-like cells activated in vitro for tumor cell killing with lymphokine (LK) and lipopolysaccharide (LPS). Treatments of these cells with optimal doses of stimulants, which evoked the development of cytolytic activity, ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90040-7
更新日期：1986-07-01 00:00:00
