Abstract：
:This study examined the characteristics of functional anergy of natural killer cells (NK) following their interaction with target cells. Purified NK cells were cocultured with K562 for 15 min or 4 hr to allow for binding of targets to NK cells. The resulting NK-target conjugates were then dissociated by EDTA, and the unbound NK cells were separated from the targets by flow cytometry and cell sorting. Compared to untreated NK cells, the K562-dissociated NK cells were inhibited for cytotoxic function as assessed by the 51Cr release assay and by the single killer frequency assay and also responded poorly following activation by IL-2 or IFN-alpha. The inactivated NK cells had a diminished ability to reform conjugates with the target cells. Following cell sorting of the NK subsets, the conjugate subset had the least cytotoxic activity when compared to both the free NK subset or the unfractionated NK population. The IL-2 response observed with the unfractionated anergic NK cells was found to be due to the activation of the NK free cell subset while the conjugate subset was poorly responsive to IL-2. The cell surface CD16, CD2, and CD56 antigen expression was downmodulated in the conjugate subset but not in the free cells. However, the CD69 surface expression was significantly upregulated on the surface of the NK conjugate subset and was potentiated following treatment with IFN-alpha and IL-2. These results demonstrate that target-mediated anergy of NK cells is restricted to the NK-target conjugate subset while sparing the remaining free cell subset. Further, the findings demonstrate that the anergic NK cells express the phenotype CD16dimCD2dimCD56dimCD69brightCD11bbright.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Jewett A,Bonavida Bdoi
10.1016/0008-8749(95)80013-9subject
Has Abstractpub_date
1995-01-01 00:00:00pages
91-7issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(95)80013-9journal_volume
160pub_type
杂志文章abstract：:The glycobiology of the immune response is a topic that has garnered increased attention due to a number of key discoveries surrounding IgG function, the specificity of some broadly neutralizing anti-HIV antibodies, cancer immunoregulation by galectin molecules and others. This review is the opening article in a Speci...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2018.04.010
更新日期：2018-11-01 00:00:00
abstract：:Class I antigens are necessary for the recognition of tumor cells by cytotoxic T lymphocytes (CTL). The line 1 lung carcinoma is a spontaneous murine tumor deficient in class I antigen expression. Consistent with this, line 1 cells are highly metastatic in vivo. We investigated whether increasing class I antigen expre...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90134-d
更新日期：1990-05-01 00:00:00
abstract：:Within the lymphoid compartment, CD8 is expressed either as an alphaalpha homodimer or as an alphabeta heterodimer. Prior functional characterization of CD8alpha transfectants has demonstrated that CD8alphaalpha homodimers can reconstitute T cell responses in the absence of the CD8beta subunit. In order to now examine...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1412
更新日期：1999-02-01 00:00:00
abstract：:The long-term exposure of mice to superantigen SEA using a mini-osmotic pump (SEA pump) induced a long-lasting expansion of Vbeta3+ CD4+ T cells with T helper (Th) 2 cell-type properties. Removal of the SEA pump 10 days after pump implantation did not significantly alter the level of Vbeta3+ CD4+ T cell expansion/main...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.09.002
更新日期：2007-08-01 00:00:00
abstract：:The loss of thymic function with age may be due to diminished numbers of T-cell progenitors and the loss of critical mediators within the thymic microenvironment. To assess the molecular changes associated with this loss, we examined transcriptomes of progressively aging mouse thymi, of different sexes and on caloric-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.03.008
更新日期：2007-01-01 00:00:00
abstract：:The primary and secondary responses to Tnp-Ficoll, a class 2 thymus-independent antigen, were assessed in various inbred strains of mice. The eventual implication of H-2 or IgH linked genes was searched for. Contrasting with our previous reports using Tnp-LPS, a class 1 thymus-independent antigen, no homologous memory...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90135-7
更新日期：1984-08-01 00:00:00
abstract：:The primary objective of this study was to determine whether the n-3 PUFA-mediated changes in host response to a Listeria monocytogenes infection (e.g., cytokine production and bacterial clearance) were dependent upon neutrophils. Balb/c mice were fed one of two semi-purified diets that contained either 0 or 41 g of n...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2005.07.003
更新日期：2005-05-01 00:00:00
abstract：:A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1279
更新日期：1998-05-01 00:00:00
abstract：:Antigen-specific signal transduction leading to IL2 induction and secretion in the T cell line 171 is augmented by association of p56lck with CD4. Although no change in cytoplasmic calcium level ([Ca2+]i) was detectable during antigen-specific signal transduction of 171-CD4+ cells, IL2 induction was inhibited by FK506...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1255
更新日期：1994-10-01 00:00:00
abstract：:Facilitating cells (FC) are bone marrow-derived cells that facilitate allogeneic hematopoietic stem cell (SC) engraftment and induce transplantation tolerance without causing graft vs. host disease. Although there is evidence for FC directing the development of FoxP3+CD4+CD25+ regulatory T cells, the specific FC subse...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.03.003
更新日期：2008-01-01 00:00:00
abstract：:Purified monomorphic monoclonal antibodies against Class I and Class II antigens in the inhibition of in vitro allogenic response were assayed. As expected, anti-Class II antibodies are highly inhibitory when used in concentrations greater than 5 micrograms/ml in MLRI and 50 micrograms/ml in MLRII. Surprisingly, anti-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90078-3
更新日期：1983-07-15 00:00:00
abstract：:To investigate the consequences of food allergy in adipose tissue and metabolism, we used a murine model in which mice have been sensitized subcutaneously with ovalbumin and further received antigen-containing diet. Allergic mice presented a significant weight loss 7 days after oral challenge with a concomitant decrea...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2011.05.008
更新日期：2011-01-01 00:00:00
abstract：:Participation of IE antigens (Ag) in immune response as the transplantation Ag was examined. IE- B10.A(4R)(4R; Kk, IAk, IE-, Db) mice could not reject skin graft from IE Ag alone-disparate B10.A(2R) (2R; Kk, IAk, IEk, Db) mice despite intravenous (iv) injection of 2R spleen cells (SC) before or after skin grafting, in...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90037-p
更新日期：1992-09-01 00:00:00
abstract：:We investigated the anti-Fas antibody-induced cell death in two different types of human cell lines, U937 and B104. IFN-gamma increased the surface expression of Fas antigen and susceptibility to anti-Fas Ab-induced cell death of B104 and U937 cells. Anti-Fas Ab-induced death of U937 and B104 cells required neither a ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1016
更新日期：1994-01-01 00:00:00
abstract：:We have examined signal transduction via membrane IgM (mIgM) in resting and cycling human B cells. Crosslinking mIgM on all of the cell types studied transduced a signal through the phosphatidylinositol pathway, producing inositol 1,4,5-trisphosphate and release of intracellular free calcium. These second messengers w...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90156-j
更新日期：1992-05-01 00:00:00
abstract：:Oxidants, heavy metals, and heat shock, collectively known as stress stimuli, induce the synthesis of a variety of proteins, termed stress proteins, and enhance glucose uptake. In this study, we have demonstrated that stress stimuli enhance protein tyrosine phosphorylation (PTyr-P), modulate protein tyrosine phosphata...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90319-k
更新日期：1992-11-01 00:00:00
abstract：:The cellular requirements for the in vitro induction of antigen-specific suppressor T cells were examined. Previous reports indicated that Ia-bearing macrophages and anti-idiotypic B cells are required as accessory cells to facilitate the generation of suppressor effector (TS3) cells which regulate the response to the...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90242-0
更新日期：1988-10-15 00:00:00
abstract：:The myelin-associated stress protein alphaB-crystallin triggers strong proliferative responses and IFN-gamma production by human T cells and it is considered a candidate autoantigen in multiple sclerosis. In this study we examined the capacity of alphaB-crystallin or peptides derived thereof to induce experimental aut...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2000.1698
更新日期：2000-09-15 00:00:00
abstract：:Monoclonal-nonspecific suppressor factor (MNSF) is a lymphokine derived from murine T cell hybridoma. The target tissues are both LPS-stimulated B cells and Con A-stimulated T cells. Since the action of MNSF may be mediated by its binding to specific cell surface receptors, we characterized the mode of this binding. T...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90271-r
更新日期：1990-10-15 00:00:00
abstract：:Abnormalities in immune response play a major role in the increased susceptibility to infection after hemorrhage and trauma. Infections occurring after injury often originate in the intestine. In order to determine the effects of hemorrhage on intestinal B cell function, we examined hemorrhage-induced alterations in a...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90015-j
更新日期：1990-06-01 00:00:00
abstract：:Interleukins play a role in the process of T-cell development and, like other cytokines, seem able to modulate apoptosis. Interleukin-2 has been reported to inhibit apoptotic cell death of thymocytes induced in vitro by either activation of CD3/TCR complex or treatment with glucocorticoid hormone. We demonstrate here ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1005
更新日期：1993-01-01 00:00:00
abstract：:Study of primary immune responses in leprosy has been limited, since disease becomes manifest long after infection or is not detectable. To study primary immune responses, we immunized in vitro human peripheral blood mononuclear cells from unexposed individuals using phenolic glycolipid 1 (PGL-1), an important water-i...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90058-j
更新日期：1991-10-01 00:00:00
abstract：:Latex microspheres-based artificial antigen-presenting cell constructs (aAPCs) are proved to be valuable tools to expand T cells ex vivo for adoptive cell therapy, but little is known about their potential for active immunization. In this report, HLA-A2/peptide tetramers were generated and co-coated with anti-mouse CD...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.07.002
更新日期：2007-05-01 00:00:00
abstract：:The activation of large granular lymphocytes (LGLs)/natural killer (NK) cells with interleukin-2 (IL-2) has been shown to increase the ability of these cells to lyse NK-resistant tumor target cells. Activated LGLs, termed LAK (lymphokine-activated killer) cells, have been demonstrated to be of therapeutic value in viv...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90132-b
更新日期：1990-05-01 00:00:00
abstract：:The proapoptotic caspase adaptor protein (PACAP) is involved in cell-cycle regulation and promotes apoptosis. Both MGC29506 and PACAP are isoforms of the MGC29506 gene and are generated by differential splicing of the alternative splice-acceptor. In studying PACAP, we inadvertently constructed the eukaryotic expressio...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.01.006
更新日期：2013-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) have been used to repair injured tissues through immune-suppression and/or cell replace mechanisms. However, a significant barrier to MSC therapy is insufficient MSC engraftment in injured tissues after systemic administration. Here, we report that cell surface, total protein, and mRNA le...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.02.001
更新日期：2013-01-01 00:00:00
abstract：:The activation by concanavalin A (Con A) of murine lymphocytes derived from aged animals is impaired, as demonstrated by lower IL-2 synthesis, display of fewer IL-2 receptors, and less [3H]thymidine incorporation relative to similarly treated cells from young animals. The ability of the phorbol ester, phorbol 12-myris...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90250-x
更新日期：1988-06-01 00:00:00
abstract：:Osteopontin (OPN) expression in tumors is associated with more aggressive tumor growth; however, several studies have suggested that OPN as a host protein can regulate tumor growth as well. OPN is produced by macrophages and T cells, and reportedly modifies macrophage function. Here, we have investigated the effect of...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2004.01.001
更新日期：2004-01-01 00:00:00
abstract：:Over the last three decades it has become increasingly clear that monocytes, originally thought to have fixed, stereotypic responses to foreign stimuli, mediate exquisitely balanced protective and pathogenic roles in disease and immunity. This balance is crucial in core functional organs, such as the central nervous s...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2014.07.002
更新日期：2014-09-01 00:00:00
abstract：:Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is caused by mutations in the AIRE gene. Murine studies suggest that AIRE controls thymic expression of tissue-restricted antigens, its absence allowing nonselected autoreactive cells to escape. We tested this in humans using the TCRβ CDR3 length ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.03.005
更新日期：2015-06-01 00:00:00
