Abstract:
:Study of primary immune responses in leprosy has been limited, since disease becomes manifest long after infection or is not detectable. To study primary immune responses, we immunized in vitro human peripheral blood mononuclear cells from unexposed individuals using phenolic glycolipid 1 (PGL-1), an important water-insoluble antigenic constituent of Mycobacterium leprae. PGL-1, encapsulated in liposomes, induced lymphoproliferation or, less frequently, suppression of lymphoproliferation in 11-day lymphocyte cultures. The primary lymphocyte responses resembled those elicited with keyhole limpet hemocyanin (KLH). HLA-DR2 expression, associated with tuberculoid leprosy, did not influence the outcome of in vitro sensitization. The association of HLA-DR2 and tuberculoid leprosy is not explained by differential ability to generate primary lymphoproliferative responses to PGL-1 or KLH. We have extended in vitro sensitization methodology to include a water-insoluble antigen in antigen-bearing liposomes. This methodology is potentially useful for studies of immunogenetics and immunopathology, and for vaccine research.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Schauf V,Holobaugh P,Miller P,Mittal Kdoi
10.1016/0008-8749(91)90058-jsubject
Has Abstractpub_date
1991-10-01 00:00:00pages
81-7issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(91)90058-Jjournal_volume
137pub_type
杂志文章abstract::Bcl-2 proto-oncogene is expressed in normal lymphoid tissues and its role has been suggested to involve the selection of B and T cells. Here we report three-color flow cytometric studies on the expression of bcl-2 protein in the thymus and periphery. Bcl-2 was expressed in the majority of T and B cells and down-regula...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1127
更新日期:1994-05-01 00:00:00
abstract::CD40--CD40L interactions between resting B cells and activated T cells are essential for germinal center formation. It has been shown that CD40L can induce both Fas expression and susceptibility to Fas-mediated killing in B cells, while anti-Ig can partially rescue B cells from Fas-mediated killing. However, the intra...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0260
更新日期:1996-10-10 00:00:00
abstract::We have performed immunoperoxidase staining on cryostat tissue sections and immunofluorescence analysis on cell suspensions to identify cells expressing the alpha/beta T cell antigen receptor during ontogeny and adult life in chickens. We used the mouse monoclonal antibody, TCR2, which was previously shown to recogniz...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90079-7
更新日期:1990-01-01 00:00:00
abstract::Mycobacterium bovis BCG and its subcellular components (bacterial extract, culture filtrate, purified protein derivative, and muramyl dipeptide MDP) are potent in vitro IL-6 inducers in spleen cell cultures from uninfected and BCG-infected BALB/c mice. Both plastic adherent and nonadherent spleen cells are capable of ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90071-i
更新日期:1991-10-01 00:00:00
abstract::The effect of the addition of dialyzable leukocyte extract (DLE)(transfer factor) to tuberculostatic drugs in the treatment of superinfected fistulating tuberculosis of bones and joints was evaluated in a controlled study. Eleven patients whose disease had persisted for a mean of 20 +/- 4.8 years and had proved to be ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90091-1
更新日期:1984-03-01 00:00:00
abstract::We have investigated the ability of interleukin-10 (IL-10) to modulate murine intrathymic T cell differentiation using a fetal thymic organ culture (FTOC) model. Addition of as little as 11 ng of recombinant murine IL-10 (mIL-10) per day produced a significant increase in the proportion and number of gamma delta-TCR+ ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1105
更新日期:1994-04-15 00:00:00
abstract::Semaphorins are important regulators of peripheral T and B-cell mediated immune responses in mice and humans. Modulatory roles of semaphorins in T cell development are also being characterized. We carefully analyzed the gene expression and protein levels of semaphorins 4A, 4D, and 7A at various developmental stages of...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.03.006
更新日期:2013-02-01 00:00:00
abstract::To understand the role of cAMP during macrophage activation, we investigated the effects of various cAMP analogues in the induction of nitric oxide (NO) synthesis in murine peritoneal macrophages. Intracellular cAMP modulators such as N6,O2'-dibutyryl cyclic AMP (DB-cAMP), 8-bromo-cAMP, or 8-chloro-cAMP had no effect ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1144
更新日期:1997-07-10 00:00:00
abstract::An important subset in regulating antitumor immunity is the maturation and accumulation of intratumor dendritic cells (DCs), inducing potent T cell cytotoxicity. In this study, we explored how the soluble abundant high-mobility group box 1 protein (HMGB1) affected DC activation and retention within lung cancers, and i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.08.011
更新日期:2019-09-01 00:00:00
abstract::Rotavirus infection of permissive cells is a multi-step process that requires interaction with several cell surface receptors. Integrins alpha2beta1, alpha4beta1, alphaXbeta2, and alphavbeta3 are involved in the attachment and entry into permissive cells for many rotavirus strains. However, possible roles of known par...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.08.025
更新日期:2005-07-01 00:00:00
abstract::Acting through CD14 and TLR4/MD-2, lipopolysaccharide (LPS) triggers strong pro-inflammatory activation of macrophages, which, if not appropriately controlled, may lead to lethal septic shock. Therefore, numerous mechanisms of negative regulation of responses to LPS exist, but whether they include down-regulation of L...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.11.008
更新日期:2017-02-01 00:00:00
abstract::Stroke is the leading cause of disability in the United States. Sex differences, including smaller infarcts in females and greater involvement of immune-mediated inflammation in males may affect the efficacy of immune-modulating interventions. To address these differences, we sought to identify distinct stroke-modifyi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.06.003
更新日期:2017-08-01 00:00:00
abstract::Blood and tissue eosinophilia is obtained when mice pretreated with cyclophosphamide (CY) and sensitized with picryl chloride are challenged on each ear lobe on day 13. To gain important insights into the cellular mechanisms involved in CY-induced eosinophilia in the contact-sensitivity reaction, we examined the cytok...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1677
更新日期:2000-08-01 00:00:00
abstract::The murine Ly-6A cell surface antigen is normally present on a minor subset of mature T cells. This marker has been shown to become highly expressed on mitogen-activated T cells. We found that expression of Ly-6A is also markedly increased in resting T cells by incubation with IFN-alpha/beta or IFN-gamma. Here, we com...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90083-9
更新日期:1987-04-01 00:00:00
abstract::It is becoming increasingly clear that regulatory T cells (Treg cells) in specific tissues are important parts of immune system. Tissue-resident Treg cells, which are largely Foxp3-expressing CD4(+) Treg cells, are distinct from one another and conventional Treg cells, and have tissue-specific phenotype and function. ...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2015.06.005
更新日期:2015-09-01 00:00:00
abstract::We report experiments attempting to optimize the proliferative response of human B cells to rabbit anti-immunoglobulin antibody (RAHIg)-linked beads (anti-Ig beads). By choosing polyacrylamide beads of small size (3 micron) and coupling anti-Ig to them at high concentrations, beads were obtained which were both B-cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90231-5
更新日期:1986-04-15 00:00:00
abstract::As an atypical guanine nucleotide exchange factor (GEF), Dock5 has been extensively studied in cellular functions. However, the role of Dock5 on B-cell immunity still remain elusive. In this study, we generated a Dock5 knockout mouse model to study the effect of Dock5 deficiency on B cell development, differentiation ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2019.01.001
更新日期:2019-03-01 00:00:00
abstract::The effect of programmed death 1 (PD-1) on cytomegalovirus (CMV)-specific T cells has not been thoroughly examined. We evaluated the involvement of exhausted CMV-specific T cells in persistent CMV infection after allogeneic hematopoietic stem cell transplantation (HSCT). CMV-specific CD8+ T cells obtained from an HLA-...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2014.02.007
更新日期:2014-03-01 00:00:00
abstract::This report compares the ability of cyclosporin A and FK-506 to inhibit human T cell activation triggered via cell surface molecules that utilize different intracellular processes. We stimulated highly purified peripheral blood T lymphocytes with mitogens (Con A and PHA), ionomycin + PMA, or monoclonal antibodies spec...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90103-i
更新日期:1991-04-01 00:00:00
abstract::We previously reported that protein tyrosine phosphatase non-receptor type 3 (PTPN3), which is upregulated in activated lymphocytes, acts as an immune checkpoint. However, the mechanism by which PTPN3 expression is enhanced in activated lymphocytes is unknown. In this study, we analyzed the mechanism of PTPN3 expressi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104237
更新日期:2020-12-01 00:00:00
abstract::Vaccination can elicit CD8(+) cytotoxic T lymphocytes (CTL) that recognize peptides presented by class I MHC molecules. Relatively little is known, however, about the genetic factors that shape the repertoire of T cell clonotypes responding to any given epitope. We report here that H-2(b) mice immunized with a plasmid...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1338
更新日期:1998-08-25 00:00:00
abstract::Following interaction of the random polymer (Glu60,Phe40)n (GPhe) with antigen-presenting accessory cells (APC), unusual costimulatory activities were noted in several murine T cell systems. When GPhe, in contrast with other random copolymers (GT,GL), was added during "inhibition" and T cell "repertoire" studies as a ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90198-z
更新日期:1990-08-01 00:00:00
abstract::Despite significant improvements in the overall survival of patients with multiple myeloma (MM) over the past 15 years, the disease remains incurable. Treatment options are limited for patients who have relapsed or are refractory to immunomodulatory drugs (IMiDs), proteasome inhibitors, and monoclonal antibodies. In t...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2018.10.003
更新日期:2018-12-01 00:00:00
abstract::Previously, we reported that human B lymphocytes can be stimulated by trinitrophenylated Brucella abortus (TNP-Ba) in vitro to generate T-independent (TI) hapten specific plaque-forming cells (PFC). Furthermore we showed that addition of Con A on Day 3 of culture enhanced the anti-TNP response. In this report the char...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90245-4
更新日期:1984-05-01 00:00:00
abstract::The phenotypic and functional relationships among the various B-cell subsets is of importance for better understanding studies of the immune system. In this report, the realm of B cells encompassed by Lyb 5, Ly 1, and xid has been examined through the use of the alloantiserum anti-Lyb 5, the unique functional properti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90077-2
更新日期:1985-04-15 00:00:00
abstract::Sialoadhesin (Sn) is a surface receptor expressed on macrophages in steady state conditions, but during inflammation, Sn can be upregulated both on macrophages and on circulating monocytes. It was shown for different species that Sn becomes internalized after binding with monoclonal antibodies. These features suggest ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.11.009
更新日期:2017-02-01 00:00:00
abstract::With old age (∼2y old), mice show substantial differences in B cell composition within the lymphoid tissues. In particular, a novel subset of IgM+ CD21/35lo/- CD23- mature B cells, the age-associated B cells or ABC, increases numerically and proportionately. This occurs at the expense of other B cell subsets, includin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.04.008
更新日期:2017-11-01 00:00:00
abstract::Immunization with synthetic mRNA encoding tumor-associated antigens is an emerging vaccine strategy for the treatment of cancer. In order to prevent mRNA degradation, promote antigen-presenting cells antigen presentation, and induce an anti-tumor immune response, we investigated the nasal administration of mRNA vaccin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104143
更新日期:2020-08-01 00:00:00
abstract::A variant nonkiller (K-) T-cell clone derived from the MHC nonrestricted killer (K+) cell line TALL-103/2 (CD3/TCR gamma delta +) was studied to determine whether its lytic defects were at the tumor-binding or post-binding level. The two TALL-103/2 clones were found to display similar mRNA expression of TCR/CD3 comple...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1212
更新日期:1995-10-15 00:00:00
abstract::To improve the engraftment of human peripheral blood lymphocytes (PBL) to severe combined immunodeficient (SCID) mice, and to elucidate the factors which prevent the PBL's survival, we treated SCID mice with mAb, which neutralizes murine IFN-gamma's ability to activate cell-mediated immunity. Compared with untreated m...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1238
更新日期:1998-01-10 00:00:00