Abstract:
:We have addressed the differential regulatory properties that IFN-gamma and IL-4 exert on macrophage (M phi) subpopulations. For this purpose, Thyoglicolate-, Peptone-, and Con A-elicited M phi, as well as bone marrow-derived M phi and P388D1 cells, were cultured in the presence of either IFN-gamma or IL-4. The expression of LFA-1, Mac-1, and Mac-2 after this treatment was studied by FACS analysis. We have found that these surface molecules are differentially modulated by the two lymphokines, depending on the M phi subpopulation studied. Mac-1 is upregulated only in Thyoglicolate-elicited cells after treatment with IFN-gamma, while no change in the expression of Mac-2 was observed in any of the groups. LFA-1 is upregulated by IFN-gamma in Thyoglicolate- and bone marrow-derived M phi and P388D1 cells, while IL-4 does not induce LFA-1 on these cells. Interestingly, however, we have observed the reverse situation on Con A-elicited M phi, where a strong induction of LFA-1 is achieved by treatment of the cells with IL-4, while IFN-gamma does not modify the expression of this antigen. Our results obtained with the lymphokine-stimulated M phi are interpreted in the context of functionally induced M phi subpopulations, which might be regulated by either Th1 or Th2 CD4+ T cells. Thyoglicolate-elicited M phi may represent the in vitro equivalent of a M phi subpopulation regulated in vivo by Th1 cells while Con A-elicited M phi could be the equivalent of a subpopulation regulated by Th2 cells.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Molina IJ,Huber BTdoi
10.1016/0008-8749(91)90347-esubject
Has Abstractpub_date
1991-04-15 00:00:00pages
241-8issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(91)90347-Ejournal_volume
134pub_type
杂志文章abstract::Antigen processing in B cells is initiated by antigen binding to the surface B cell antigen receptor (BCR). The BCR is a signaling receptor which also functions to endocytose bound antigen for subsequent intracellular processing and presentation with class II molecules. Previously, using subcellular fractionation, we ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1264
更新日期:1998-02-25 00:00:00
abstract::Within lymphopenic recipients, naïve T cells undergo proliferation that is induced by homeostatic mechanisms. Earlier studies have demonstrated that commensal antigens play a key role in inducing the proliferation. However, a relative contribution of endogenous self antigens in this process has not been formally inves...
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abstract::Activated natural killer T (NKT) cells produce a broad range of cytokines, including IL-4 and IFN-gamma, that determine immunomodulatory functions in various animal models. In this report, we show that a well-known proinflammatory cytokine, IL-17 is also produced by a distinct population of NKT cells upon TCR stimulat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.03.004
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90327-8
更新日期:1991-04-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90059-x
更新日期:1983-07-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1038
更新日期:1995-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1985-11-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90117-2
更新日期:1989-11-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1999-03-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90149-6
更新日期:1991-11-01 00:00:00
abstract::PD-L1 have been identified as the ligand for PD-1, and shown to play a role in the regulation of immune responses. In the present study, we investigated whether overexpressing PD-L1 on islet beta cells could induce negative regulation in primary and primed allogeneic lymphocyte response. pPD-L1-EGFP or pEGFPn1 were tr...
journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.09.010
更新日期:2015-11-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90060-8
更新日期:1987-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1139
更新日期:1995-08-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90291-8
更新日期:1989-10-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90342-3
更新日期:1984-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
abstract::The anti-spirochete T cell immune response to immunization with Borrelia burgdorferi was investigated. The cellular immune response to vaccination of immunocompetent BALB/c mice was characterized initially in vitro by assay of the proliferative response of primary lymph node cells to B. burgdorferi sonicate. Subsequen...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1073
更新日期:1995-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1021
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1984-01-01 00:00:00
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更新日期:1989-06-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1113
更新日期:1995-07-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90323-h
更新日期:1992-11-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1571
更新日期:1999-11-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1999-08-25 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90105-k
更新日期:1991-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1996-11-25 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90037-2
更新日期:1987-02-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00