Abstract:
:During the course of differentiation in the thymus, precursor T cells are negatively selected by a self-tolerance mechanism or positively selected to acquire restriction specificity to self major histocompatibility complexes. We investigated the process of T cell differentiation and those selections using a fetal thymus organ culture with or without cyclosporine A. The agent blocked the maturation step from CD4+8+ double positive cells to mature CD4-8+ or CD4+8- single positive cells. On the other hand, the agent did not inhibit the development of CD3+4-8- T cell receptor (TCR)alpha beta- cells, which were supposed to be T cells bearing gamma delta-TCR chains. These results suggest that the development of thymocytes bearing alpha beta- or gamma delta-TCR chains differ in requirement for thymocyte-stromal cell interaction.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Matsuhashi N,Kawase Y,Suzuki Gdoi
10.1016/0008-8749(89)90291-8subject
Has Abstractpub_date
1989-10-15 00:00:00pages
307-15issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(89)90291-8journal_volume
123pub_type
杂志文章abstract::Within the lymphoid compartment, CD8 is expressed either as an alphaalpha homodimer or as an alphabeta heterodimer. Prior functional characterization of CD8alpha transfectants has demonstrated that CD8alphaalpha homodimers can reconstitute T cell responses in the absence of the CD8beta subunit. In order to now examine...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1412
更新日期:1999-02-01 00:00:00
abstract::Our previous studies show that growth hormone overexpression (GHo) upregulates the expression of the IGF-1R and IGF-2R resulting in the protection of the EL4 lymphoma cell line from apoptosis. In this study, we report that GHo also increases TGF-beta1 protein expression measured by luciferase promoter assay, Western a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.06.003
更新日期:2006-03-01 00:00:00
abstract::G proteins are membrane-bound molecules involved in coupling of surface receptors with signal transduction effector systems in multiple cell types including T lymphocytes. Given that mature T cells which lack antigen receptors (CDl-Ti) are refractory to stimulation through CD2 or other accessory molecules, T cell rece...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90050-2
更新日期:1990-07-01 00:00:00
abstract::Engraftment of stem cell-enriched donor marrow implanted in the thymus of a foreign host might facilitate acceptance of donor-specific organ or tissue grafts. To test this hypothesis, allogeneic and xenogeneic CD34+ marrow cells from unrelated adult male baboons and humans were injected intrathymically in eight infant...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1194
更新日期:1997-11-01 00:00:00
abstract::Inhibition of DPP IV has been shown to abrogate the stress-related increase in murine abortions and a concomitant increase in gamma-interferon. The aim of the present study was to investigate a potential impact of the DPP IV inhibitor Isoleucine Cyanopyrrolidide on the expression of surface antigens involved in T-cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(03)00028-5
更新日期:2002-12-01 00:00:00
abstract::Ferric ammonium citrate (FAC) is nonmitogenic for human peripheral blood mononuclear cells (PBM) but has a potent mitogenic activity in the presence of IL-2. FAC in the presence of IL-2 increases the number of human peripheral blood mononuclear cells (PBM) expressing receptors for IL-2 and transferrin. FAC also marked...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90105-k
更新日期:1991-04-01 00:00:00
abstract::Some further aspects of B-lymphocyte-promotor factor (B-LPF) activity have been studied. This activity was present in the supernatants of certain helper-T-cell lines, and it induced polyclonal activation of Ig+ B cells into Ig-secreting cells. It was found that B-LPF induced polyclonal, terminal B-cell differentiation...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90067-x
更新日期:1985-04-15 00:00:00
abstract::The regulation of the migratory capacity of Percoll-purified large granular lymphocytes (LGL) into nitrocellulose filters was studied in a 2-hr assay with the use of modified Boyden chambers. Compounds that stimulate the natural killer cytotoxic function of LGL, such as interferons (natural beta, recombinant alpha A, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90198-x
更新日期:1986-08-01 00:00:00
abstract::Chronic allotype suppression was generated in M-1 (C mu), G-1 (C gamma) heterozygous B14 line chickens either by embryonal injection or by maternal transfer of anti-M-1b allotype antibodies. Using a sensitive radioimmunoassay to detect the suppressed IgM-1b, a striking disparity was observed between the degree of supp...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90240-5
更新日期:1984-01-01 00:00:00
abstract::The oral administration of myelin basic protein (MBP) to Lewis rats prior to an encephalitogenic challenge resulted in total inhibition or a significant delay in the onset of experimental autoimmune encephalomyelitis (EAE). In vitro lymphocyte proliferative responses to MBP were significantly decreased in MBP-fed rats...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90305-x
更新日期:1988-04-01 00:00:00
abstract::T cell hybridomas that are deathless upon TCR crosslinking were generated from lpr mice. The deathless hybridomas (1.4 and 5D5) expressed extremely low Fas even after anti-CD3 activation, whereas activation-induced cell death (AICD) was observed for Fas-expressing hybridomas. The deathless hybridomas were activated to...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0039
更新日期:1996-02-01 00:00:00
abstract::In test in vitro, four sulfated lycium barbarum polysaccharides (sLBPSs) with different degrees of sulfation (DS), sLBPS0.7, sLBPS1.1, sLBPS1.5 and sLBPS1.9, were added into cultured chicken peripheral lymphocytes and the changes of lymphocytes proliferation were compared by MTT assay taking the non-modified LBPS as c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.04.001
更新日期:2010-01-01 00:00:00
abstract::Ligation of CD40 using anti-CD40 or soluble CD40-ligand activates numerous intracellular kinases which transduce signals to the nucleus. The nature whereby these signaling events are coupled to distal functional events in B cells is poorly understood. In this study, using anti-CD40 monoclonal antibodies which recogniz...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1645
更新日期:2000-05-01 00:00:00
abstract::The administration of low-density lipoproteins from chicken egg yolk (EyLDL) to mice induced T-dependent antibodies against bromelain-treated mouse erythrocytes (BrMRBC). The anti-BrMRBC antibodies produced antigen specifically by EyLDL appear to be identical to the antibodies produced polyclonally by lipopolysacchari...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90195-5
更新日期:1988-09-01 00:00:00
abstract::Permanent graft acceptance was induced with cyclosporine A in a low responder rat strain combination PVG (RT1(c)) to DA (RT1(av1)) of cardiac transplants. Challenge (alloimmunization) with donor and third-party WF (RT1(u)) cells was performed and followed by the analysis of alloreactive cytotoxicity. The cytotoxic T l...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1353
更新日期:1998-10-10 00:00:00
abstract::Histidine-proline-rich glycoprotein (HPRG) is a plasma protein which binds to a specific receptor on T-lymphocytes and represses T-cell activation and proliferation. In the presence of Concanavalin A (Con A), HPRG causes human T-lymphoblastic MOLT-3 cells and a fraction of normal human peripheral blood lymphocytes to ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1311
更新日期:1993-12-01 00:00:00
abstract::The molecular mechanism responsible for the age-dependent decline of natural killer (NK) cell response was examined. The responses of NK cells to poly(I:C), Con A, and LPS were compared between the young and the aged animals. We observed that both basal and induced NK cell activity declined with age. To further determ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0271
更新日期:1996-11-01 00:00:00
abstract::PHA stimulation of both freshly isolated and IL-2-dependent cultured T cells induced a rapid rise in intracytoplasmic calcium concentration. Chelation of extracellular calcium with EGTA resulted in a failure of PHA to induce a rise in intracytoplasmic calcium, resulting in the fresh T cells in an inhibition of IL-2 pr...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90060-3
更新日期:1988-01-01 00:00:00
abstract::Estrogen displays potent immunoregulatory properties. In the present study we investigated the in vivo effect of estrogen on natural killer (NK) cell cytotoxic activity in several mouse strains. After castration, 17 beta-estradiol was administered in low or high doses. After 3-6 weeks of hormone administration an in v...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1262
更新日期:1994-10-01 00:00:00
abstract::The quantitative organ distribution and tissue microenvironment positioning of radioisotopically labeled cloned T cells were characterized. Intravenous (iv) injection of 51chromium (51Cr)-labeled, long-term cultured cloned T-helper cells and cells from several cloned cytolytic T-lymphocyte lines (CTLL) resulted in poo...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90349-0
更新日期:1983-02-15 00:00:00
abstract::In this study we investigated whether T cells expressing high or low levels of CD62L were differentially susceptible to the T cell chemokine lymphotactin. We found that lymphotactin induced preferential migration of antigen-specific (CD62L(lo)) T cells over the nonspecific (CD62L(hi)) T cells in vitro and in vivo. The...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1790
更新日期:2001-05-01 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) play a major role in modulating immune response, but only a few reports focused on MDSCs in the liver of sepsis states. Here, we investigated the changes in MDSCs in liver of the cecal ligation and puncture (CLP) mice. The results of flow cytometry showed that MDSCs accumulate ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.08.005
更新日期:2012-09-01 00:00:00
abstract::Previous work in our laboratory established that individual complement components can be regulated in vivo by administration of specific antibody or immunocompetent cells to newborns and in vitro by administration of specific antibody to cultured peritoneal macrophages or splenic fragments. Antibody-induced suppressio...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90337-5
更新日期:1985-11-01 00:00:00
abstract::RELT is a recently identified Tumor Necrosis Factor Receptor that possess two homologues in humans named RELL1 and RELL2. We investigated whether RELT and its homologues could induce cellular death when transiently transfected into HEK 293 epithelial cells. Transfection of RELT family members into HEK 293 epithelial c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.10.013
更新日期:2010-01-01 00:00:00
abstract::Our strategy to use saturation mutagenesis to produce an unbiased collection of major histocompatibility class I mutants has resulted in unpredicted mutant phenotypes. First, we have shown data supporting our earlier work of the Dp20(Y27N) mutant. Allorecognition is altered at the clonal level while no variation in ly...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90020-r
更新日期:1990-06-01 00:00:00
abstract::Class II major histocompatibility antigens (Ia) play a major role in regulating T-B cell interactions; therefore, regulation of the amount of Ia on B cells may be an important point of control in the immune response. Mitogens in human and murine systems have been reported to increase Ia expression on B cells, and in t...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90108-6
更新日期:1988-02-01 00:00:00
abstract::Staphylococcus enterotoxins bind class II MHC molecules on antigen-presenting cells (APC) and stimulate T cells expressing appropriate V beta gene products. Although the role of non-TcR-associated costimulatory receptors during antigen-specific T cell stimulation has been clearly established, the involvement of costim...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1084
更新日期:1995-05-01 00:00:00
abstract::Stimulated macrophages are known to inhibit the growth of certain tumor cells. Using mouse peritoneal exudates as a source of macrophages and the mastocytoma cell line P815 as the target, the inhibition was found to depend on direct contact between the macrophages and the growing cells. Cytostatic activities were dete...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90395-3
更新日期:1986-02-01 00:00:00
abstract::Differences in gender immune response have resulted in differences in immune protection and susceptibility to inflammatory diseases. Cultured peripheral blood mononuclear cells (PBMC) are widely used in immunomodulation studies, yet the influence of gender is usually not considered. We examined the effect of in vitro ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.10.009
更新日期:2012-01-01 00:00:00
abstract::CD40 and its ligand (CD40L) regulate several cellular functions, including T and B-cell activation. The soluble form of CD40 (sCD40) antagonizes CD40/CD40L interaction. Patients undergoing hemodialysis (HD) present elevated sCD40 serum levels, which underlying molecular mechanisms are unknown. We studied sCD40 serum a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.06.007
更新日期:2012-07-01 00:00:00