Abstract：
:A previous report (G. E. Woloschak and D. Senitzer, submitted for publication) has demonstrated that mitogen-stimulated splenocytes fuse at a much higher frequency than untreated splenocytes as measured by the fusion index (a calculation of the number of nuclei in fused cells vs the total number of nuclei). A measurement of the fusion indices of NZB spleen cells provides results markedly different from those observed with splenocytes from control strains of mice--NZB spleen cells exhibit a spontaneously high fusion index. In this assay, they spontaneously display the same fusing capacity as that observed in cultures of mitogen-treated spleen cells from control strains of mice. This elevated fusion index is not affected by treatment with anti-Thy-1.2 and complement, but is abrogated by treatment with anti-immunoglobulin serum and complement. This suggests that a B cell is responsible for the high fusion index of NZB splenocytes. This high fusion index is present when using splenocytes from both male and female mice in the fusion assay, and can be observed using spleen cells from NZB mice as young as 12 days. This appears to be the result of a spontaneous B-cell activation in NZB mice.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Woloschak GE,Senitzer Ddoi
10.1016/0008-8749(83)90059-xsubject
Has Abstractpub_date
1983-07-01 00:00:00pages
164-72issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(83)90059-Xjournal_volume
79pub_type
杂志文章abstract：:Monoclonal-nonspecific suppressor factor (MNSF) is a lymphokine derived from murine T cell hybridoma. The target tissues are both LPS-stimulated B cells and Con A-stimulated T cells. Since the action of MNSF may be mediated by its binding to specific cell surface receptors, we characterized the mode of this binding. T...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90271-r
更新日期：1990-10-15 00:00:00
abstract：:In the search for its role in inflammatory joint diseases, soluble HLA-DR (sHLA-DR) was quantitated in 72 synovial fluids (SF) by a newly established immunoenzyme assay. Unlike other soluble receptors which accumulated only moderately (sCD25, sCD4) or negligibly (sHLA class I, sCD8) in the SF, SF sHLA-DR levels exceed...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2000.1729
更新日期：2000-12-15 00:00:00
abstract：:In this report, a subset of CD4(+) cells which kills autologous HLA class I positive and HLA class II negative non-small-cell lung cancer (NSCLC) cells is described. Killing was performed both by direct cytolytic mechanisms and by apoptosis. The peculiar characteristic of these effectors was their capability of lysing...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1999.1547
更新日期：1999-09-15 00:00:00
abstract：:The precise role of the granular enzyme A (granzyme A), a serine protease, in the lytic process of cytotoxic T lymphocytes (CTL) is not clear. We have recently constructed a CTL line transfected with the antisense gene of granzyme A (a-GrA). These a-GrA CTL had lower GrA activity as well as decreased lytic activities,...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0300
更新日期：1996-12-15 00:00:00
abstract：:The loss of thymic function with age may be due to diminished numbers of T-cell progenitors and the loss of critical mediators within the thymic microenvironment. To assess the molecular changes associated with this loss, we examined transcriptomes of progressively aging mouse thymi, of different sexes and on caloric-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.03.008
更新日期：2007-01-01 00:00:00
abstract：:In vivo treatment with anti-IA antibodies has been shown to induce a haplotype-specific inhibition of EAE when the disease was following passive transfer of MBP-sensitized T cells. In order to determine the mechanism by which anti-IA antibody prevents passively transferred EAE, the homing of radiolabeled cells to the ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90267-f
更新日期：1991-06-01 00:00:00
abstract：:We have studied the effects of prostaglandin E2 (PGE2) and cholera toxin, two modulators of adenylyl cyclase, and 8-bromo cAMP (8-BrcAMP) on various parameters of lymphocyte activation using the human T cell line Jurkat. Our results show that PGE2 and cholera toxin inhibit, in a dose-related manner, the phytohemagglut...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90035-p
更新日期：1990-07-01 00:00:00
abstract：:The experiments in this manuscript confirm and extend our previous finding that IL4 has minimal enhancing activity on B cell activation stimulated by anti-Ig-dextran conjugates. The absence of significant IL4-mediated enhancement is seen also under conditions which are limiting for optimal B cell proliferation. Thus, ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90275-v
更新日期：1990-10-15 00:00:00
abstract：:Evidence is presented that rat serosal mast cells and cells of the rat basophilic leukemia line, RBL-1, each contain a calcium-activated, phospholipid-dependent protein kinase. The enzymes are very similar in their activation requirements to the calcium-dependent enzymes termed protein kinase Cs in brain. The enzyme i...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90098-5
更新日期：1986-12-01 00:00:00
abstract：:The third complementarity determining region (CDR3) is the most variable part of alpha beta T cell receptors (TCR) and represents the putative antigen contacting site. To identify parameters determining the structural diversity of the CDR3 region, the CDR3 length distributions of 66 BV-J combinations in peripheral CD4...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0071
更新日期：1996-03-15 00:00:00
abstract：:Receptors for the Fc domain of IgG mediate target recognition, signal transduction, and effector functions including antibody-dependent cytolysis, phagocytosis, and phagolysosome formation. To better understand FcR-mediated functions and to identify potential therapeutic strategies, we employed cell-penetrating ("Troj...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.12.002
更新日期：2010-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) have been used to repair injured tissues through immune-suppression and/or cell replace mechanisms. However, a significant barrier to MSC therapy is insufficient MSC engraftment in injured tissues after systemic administration. Here, we report that cell surface, total protein, and mRNA le...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.02.001
更新日期：2013-01-01 00:00:00
abstract：:Although CD4(+)CD25(+) regulatory T cells are pivotal in the suppression of autoimmunity, little is known about the effect of antigen-specific regulatory T cells on the formation of atheromatous plaques. Here, we describe the induction of heat-shock protein 60 (HSP60)-specific CD4(+)CD25(high) T cells by rapamycin (RP...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.01.002
更新日期：2006-10-01 00:00:00
abstract：:Severe aplastic anemia (SAA) is a syndrome of severe bone marrow failure with high mortality. Our previous studies have demonstrated that both immature and activated DC1 increased in the bone marrow of SAA patients, and the balance of DC1 subsets shifted the stable form to active one, which might promote Th0 cells to ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.09.007
更新日期：2013-09-01 00:00:00
abstract：:Recombinant IL-4 inhibits IL-2-induced lymphokine-activated killer (LAK) cell development of PBMC. We evaluated the effect of various cytokines in reversing IL-4-mediated LAK inhibition. PBMC were cultured in IL-2 (10-1000 u/ml) with or without IL-4 (2-100 u/ml) and tested for cytotoxicity against the NK-sensitive K56...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90040-x
更新日期：1990-07-01 00:00:00
abstract：:Normal chondrocytes display susceptibility to lysis by natural killer (NK) cells and this phenomenon may play a role in some inflammatory cartilage disorders. The mechanisms of chondrocyte recognition and killing by NK cells remain unclear. Using flow cytometry and immunohistochemical staining we found that normal hum...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.08.005
更新日期：2013-09-01 00:00:00
abstract：:The humoral response to encapsulated BSA appears to be a classical TD antigen response with a high ratio of IgG to IgM, whereas that to covalently-linked antigen is more complex, characterized by an enhanced synthesis of IgM, leading to an equal production of IgM and IgG. In a recent paper, we observed that surface-li...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90266-9
更新日期：1989-10-01 00:00:00
abstract：:A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1279
更新日期：1998-05-01 00:00:00
abstract：:American cutaneous leishmaniasis (ACL) is a disease where susceptibility or resistance is dependent on T cell response. This is characterized by an increased in CD4⁺ T cells, capable of inducing opposite disease profiles, and CD8⁺ T cells, that are related to immuno protection. We characterized T lymphocytes from pati...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2012.11.006
更新日期：2012-10-01 00:00:00
abstract：:Monolayers of freshly isolated human monocytes are known to ingest particulate activators of the human alternative complement pathway. The ingestion of rabbit erythrocytes, ER, by human monocytes in serum-free medium was studied. The process is Mg2+-dependent and optimum phagocytic activity was obtained at approximate...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90024-x
更新日期：1988-05-01 00:00:00
abstract：:Spleen cells from neonatal mice belonging to strains with the I-J-b or I-J-k genotype, were treated with anti-I-Jb or anti-I-Jk antibody and complement. This reduces their suppressor cell activity as demonstrated by a decrease in the ability to suppress the mixed-lymphocyte reactivity of adult spleen cells. Injection ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90115-6
更新日期：1983-09-01 00:00:00
abstract：:Abnormalities in immune response play a major role in the increased susceptibility to infection after hemorrhage and trauma. Infections occurring after injury often originate in the intestine. In order to determine the effects of hemorrhage on intestinal B cell function, we examined hemorrhage-induced alterations in a...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90015-j
更新日期：1990-06-01 00:00:00
abstract：:We compared macrophage binding and killing of F5b cells to the binding and killing of P815 mastocytoma cells and to several other nontransformed and transformed cell lines. Formalin fixation of elicited or activated macrophages did not affect binding of F5b or 3T3 cells but did abrogate binding of P815 cells. However,...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90190-m
更新日期：1991-03-01 00:00:00
abstract：:B-cell clonal expansions (BCE) in young mice are transient, detectable for less than 4 weeks. In contrast, BCE in old mice persist more than 2 months. The greater persistence of BCE in old mice does not appear to be due to the age of the host as the survival of phenyloxazolone chicken serum albumin-induced BCE in most...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2005.01.002
更新日期：2004-09-01 00:00:00
abstract：:A spontaneously arising murine plasmacytoma, HPC-202, derived from a BALB/c.H-2b congenic mouse that lacks any detectable H-2 determinants on its cell surface is described. However, the expression of H-2 determinants is inducible by interferon-gamma. The H-2 negative cell surface phenotype permits the HPC-202 tumor to...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90114-7
更新日期：1990-04-15 00:00:00
abstract：:The proapoptotic caspase adaptor protein (PACAP) is involved in cell-cycle regulation and promotes apoptosis. Both MGC29506 and PACAP are isoforms of the MGC29506 gene and are generated by differential splicing of the alternative splice-acceptor. In studying PACAP, we inadvertently constructed the eukaryotic expressio...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.01.006
更新日期：2013-01-01 00:00:00
abstract：:Large suppressive macrophages (LSM) were induced by restimulating spleen cells from rats with experimental autoimmune myasthenia gravis (EAMG) in vitro, with the autoantigen acetylcholine receptor (AChR) in the presence of cyclosporine A. LSM, purified from these cultures, are extremely potent suppressors of AChR-stim...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1445
更新日期：1999-04-10 00:00:00
abstract：:Strong delayed-type hypersensitivity (DTH) to Babesia microti was elicited when intraerythrocytic parasites (IEP) were inoculated subcutaneously into the flank of normal mice 6 to 14 days before challenge in the ipsilateral footpad with 10(8) IEP. Intraperitoneal or intravenous administration of antigen did not sensit...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90289-3
更新日期：1986-04-01 00:00:00
abstract：:We evaluated the effects of the immunosuppressants cyclosporin A (CsA) and FK506 on the synthesis of nitric oxide (NO) induced by bacterial lipopolysaccharide (LPS) in J774 macrophages. CsA and FK506 each inhibited NO production by LPS in a concentration-dependent fashion, but the cytotoxicity was also evident at high...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1180
更新日期：1995-10-01 00:00:00
abstract：:The role of surface aminopeptidases (APs), enzymes that cleave amino-terminal residues from polypeptide chains, in the development of fetal thymocytes was studied using a murine fetal thymic organ culture (FTOC) model. FTOC AP activity was demonstrable for various amino acid-p-nitroanilide substrates, and specific inh...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1099
更新日期：1997-04-10 00:00:00
