Macrophage binding of cells resistant and sensitive to contact-dependent cytotoxicity.


:We compared macrophage binding and killing of F5b cells to the binding and killing of P815 mastocytoma cells and to several other nontransformed and transformed cell lines. Formalin fixation of elicited or activated macrophages did not affect binding of F5b or 3T3 cells but did abrogate binding of P815 cells. However, formalin fixation abrogated resident macrophage binding of F5b and 3T3 cells. Therefore, depending on the type of macrophage or target cell, formalin fixation may affect binding. Only the binding of P815 cells was dependent upon activation; macrophage binding of target cells F5b and 3T3 was not. Even though macrophages bound F5b and 3T3 cells, macrophages only mediated contact-dependent cytotoxicity against F5b cells. Macrophages did not kill 3T3 cells. Experiments also compared macrophage binding and killing of the uv-light-induced tumor cell lines 1422, 2237, and 2237a46. Only the cell line 2237a46 was susceptible to contact-dependent killing. Both 1422 and 2237 cells were resistant. In contrast, cell lines 2237a46 and 1422 were bound by activated macrophages while 2237 cells were bound poorly.


Cell Immunol


Cellular immunology


Rosenkrans CF Jr,Chapes SK




Has Abstract


1991-03-01 00:00:00














  • In vitro antitumor immune response induced by dendritic cells transduced with human livin α recombinant adenovirus.

    abstract::Transduction with recombinant, replication-defective adenoviral (rAd) vectors encoding a transgene is an efficient method for gene transfer into human dendritic cells (DCs). Livin is a good candidate for cancer immunotherapy since it is overexpressed in most common human cancers, poorly expressed in most normal adult ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Xie J,Guo X,Liu F,Luo J,Duan F,Tao X

    更新日期:2015-09-01 00:00:00

  • Analysis of B-cell subpopulations. I. Relationships among splenic xid, Ly 1+, and Lyb 5+ B cells.

    abstract::The phenotypic and functional relationships among the various B-cell subsets is of importance for better understanding studies of the immune system. In this report, the realm of B cells encompassed by Lyb 5, Ly 1, and xid has been examined through the use of the alloantiserum anti-Lyb 5, the unique functional properti...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Smith HR,Yaffe LJ,Chused TM,Raveche ES,Klinman DM,Steinberg AD

    更新日期:1985-04-15 00:00:00

  • Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro.

    abstract::The cell-averaged microviscosity of intact murine peritoneal mononuclear phagocytes in various stages of activation was assessed by quantifying fluorescent depolarization of 1,6-diphenyl-1,3,5-hexatriene. Macrophages activated in vivo with Mycobacterium bovis, strain BCG, were significantly more fluid than resident pe...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Somers SD,Yuli I,Snyderman R,Adams DO

    更新日期:1987-02-01 00:00:00

  • Anti-allergic drug olopatadine suppresses murine contact hypersensitivity and downmodulates antigen-presenting ability of epidermal Langerhans cells.

    abstract::Olopatadine hydrochloride is an H1-receptor-blocker but has other anti-allergic pharmacological potencies. We investigated whether olopatadine inhibits murine contact hypersensitivity, focussing on its modulatory action on epidermal Langerhans cells serving as antigen-presenting cells. While BALB/c mice were sensitize...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Tokura Y,Kobayashi M,Ito T,Takahashi H,Matsubara A,Takigawa M

    更新日期:2003-07-01 00:00:00

  • Expression of CD45 isoforms in fresh and IL-2-cultured tumor-infiltrating lymphocytes from basal cell carcinoma.

    abstract::Immunohistochemistry and flow cytometry analyses were performed to characterize the specific T cell subpopulations infiltrating basal cell carcinomas (BCC) and to determine their phenotypic response to in vitro expansion with IL-2. Tumor-infiltrating lymphocytes (TIL) within BCC predominantly expressed the CD45RO (act...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Nguyen QH,Moy RL,Roth MD,Yamamoto R,Tomono S,Dubinett SM

    更新日期:1993-02-01 00:00:00

  • Bacteria-derived human leukocyte interferons alter in vitro humoral and cellular immune responses.

    abstract::Cultures of gradient-purified human peripheral blood mononuclear cells (PBMC) have been employed to examine the effects of three bacteria-derived human leukocyte interferon subtypes on certain aspects of in vitro immune responses. The addition of highly purified IFN-alpha 1, -alpha 2, -alpha 2/alpha 1 to PMBC cultures...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Shalaby MR,Weck PK

    更新日期:1983-12-01 00:00:00

  • Functional impairment of CD4 T cells despite normalization of T cell number in HIV.

    abstract::Using an in vitro model, we demonstrate that when CD4 T cells from HIV infected subjects are enriched from total blood lymphocytes the immune response to antigen is augmented. However, augmentation of this response is confined to HIV infected subjects with relatively preserved CD4 T cell counts. Enriching for CD4 T ce...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Knox KS,Day RB,Kohli LM,Hage CA,Twigg HL 3rd

    更新日期:2006-07-01 00:00:00

  • Comparison of lymphokine secretion and responsiveness of human T cell clones isolated in IL-4 and in IL-2.

    abstract::Interleukin (IL)-4 has been shown to be secreted simultaneously with IL-2 and interferon (IFN)-gamma by the majority of CD4+ human T cell clones isolated and cultured using IL-2 as a growth factor. Moreover, IL-4 was found to be as efficient as IL-2 to promote the outgrowth of human T cell clones. In this study we hav...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Paliard X,de Vries JE,Spits H

    更新日期:1991-07-01 00:00:00

  • T cell receptor germline gene segments and HLA haplotypes control the length of the CDR3 of human T cell receptor beta chains.

    abstract::The third complementarity determining region (CDR3) is the most variable part of alpha beta T cell receptors (TCR) and represents the putative antigen contacting site. To identify parameters determining the structural diversity of the CDR3 region, the CDR3 length distributions of 66 BV-J combinations in peripheral CD4...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kayser C,Waase I,Weyand CM,Goronzy JJ

    更新日期:1996-03-15 00:00:00

  • Regulation of antibody production by hybridoma cultures. I. Anti-idiotype antibody-mediated down-regulation of anti-DNA antibody production by hybridoma cells.

    abstract::A series of anti-DNA antibody-producing hybridomas were obtained by fusing spleen cells from 6-month-old MRL/lpr, autoimmune prone, mice with P3X63-Ag8 myeloma cells. Rabbit anti-idiotype (id) antibodies specific for several of the hybridoma proteins were prepared. It was shown that the anti-id antibody inhibited immu...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kim YT,Puntillo E,DeBlasio T,Weksler ME,Siskind GW

    更新日期:1987-03-01 00:00:00

  • Both exogenous commensal and endogenous self antigens stimulate T cell proliferation under lymphopenic conditions.

    abstract::Within lymphopenic recipients, naïve T cells undergo proliferation that is induced by homeostatic mechanisms. Earlier studies have demonstrated that commensal antigens play a key role in inducing the proliferation. However, a relative contribution of endogenous self antigens in this process has not been formally inves...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Do JS,Foucras G,Kamada N,Schenk AF,Shaw M,Nuñez G,Paul WE,Min B

    更新日期:2012-01-01 00:00:00

  • Dock5 controls the peripheral B cell differentiation via regulating BCR signaling and actin reorganization.

    abstract::As an atypical guanine nucleotide exchange factor (GEF), Dock5 has been extensively studied in cellular functions. However, the role of Dock5 on B-cell immunity still remain elusive. In this study, we generated a Dock5 knockout mouse model to study the effect of Dock5 deficiency on B cell development, differentiation ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Chen A,Yang D,Xuan X,Miller H,Luo X,Yu J,Yang G,Wang H,Liu C

    更新日期:2019-03-01 00:00:00

  • Nonrestricted cytotoxicity mediated by interleukin 2-expanded leukocytes is inhibited by anti-LFA-1 monoclonal antibodies (MoAb) but potentiated by anti-CD3 MoAb.

    abstract::Previous results have shown that in addition to their ability to kill tumor cell lines, peripheral blood leukocytes (PBL) expanded in interleukin 2 (IL-2) can also destroy normal PBL targets. Cold target competition results show that PBL and tumor cells can be destroyed by the same population of IL-2-expanded leukocyt...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Chen BP,Malkovsky M,Hank JA,Sondel PM

    更新日期:1987-12-01 00:00:00

  • Role of class I and class II antigens in the allogenic stimulation: class I and class II recognition in allogenic stimulation; blocking of MLR by monoclonal antibodies and F(ab')2 fragments.

    abstract::Purified monomorphic monoclonal antibodies against Class I and Class II antigens in the inhibition of in vitro allogenic response were assayed. As expected, anti-Class II antibodies are highly inhibitory when used in concentrations greater than 5 micrograms/ml in MLRI and 50 micrograms/ml in MLRII. Surprisingly, anti-...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kalil J,Wollman EE

    更新日期:1983-07-15 00:00:00

  • Activation of donor-specific CTL in a tolerant recipient of cardiac allograft.

    abstract::Permanent graft acceptance was induced with cyclosporine A in a low responder rat strain combination PVG (RT1(c)) to DA (RT1(av1)) of cardiac transplants. Challenge (alloimmunization) with donor and third-party WF (RT1(u)) cells was performed and followed by the analysis of alloreactive cytotoxicity. The cytotoxic T l...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Petersson E,Qi Z,Hedlund G,Ekberg H

    更新日期:1998-10-10 00:00:00

  • Comparison of the capacity of murine and human class I MHC molecules to stimulate T cell activation.

    abstract::The mechanism underlying the apparent differences in the capacity of murine and human class I MHC molecules to function as signal transducing structures in T cells was examined. Cross-linking murine class I MHC molecules on splenic T cells did not stimulate an increase in intracellular calcium ([Ca2+]i) and failed to ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Gur H,Wacholtz MC,Lie WR,Lipsky PE,Geppert TD

    更新日期:1992-10-15 00:00:00

  • Semaphorin 4A is dynamically regulated during thymocyte development in mice.

    abstract::Semaphorins are important regulators of peripheral T and B-cell mediated immune responses in mice and humans. Modulatory roles of semaphorins in T cell development are also being characterized. We carefully analyzed the gene expression and protein levels of semaphorins 4A, 4D, and 7A at various developmental stages of...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Linder GE,Chuntova PD,McLelland BT,Añó L,Obodo UC,Crider NJ,Matthes DJ,García-Ojeda ME,Manilay JO,Chatterjea D

    更新日期:2013-02-01 00:00:00

  • Proteomics analysis reveals the interleukin-35-dependent regulatory mechanisms affecting CD8+ T-cell functions.

    abstract::Interleukin (IL)-35 strongly suppresses the immune effects of CD8+ T cells. However, the mechanisms mediating these effects are not clear. Here, we investigated the potential inhibitory mechanisms of IL-35 using proteomics technology. The changes of differentially expressed proteins (DEPs) were evaluated using gene on...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Li X,Dong Y,Tu K,Wang W

    更新日期:2020-02-01 00:00:00

  • In vitro induction of immunological tolerance.

    abstract::IL-2 was previously shown to induce cytotoxic effectors with a broad spectrum of target specificities in thymus and spleen cell cultures. This study was designed to show whether T cells activated by H-2 allogeneic cells in MLC or by syngeneic tumor cells in MLTC are also potential targets for these cytotoxic effectors...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Biasi G,Mazzocchi M,Zanovello P,Collavo D,Festenstein H

    更新日期:1989-12-01 00:00:00

  • CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes.

    abstract::Non-obese diabetic (NOD) mice develop spontaneous T-cell responses against pancreatic beta-cells, leading to islet cell destruction and diabetes. Despite high genetic similarity, non-obese resistant (NOR) mice do not develop diabetes. We show here that spleen cells of both NOD and NOR mice respond to the islet cell an...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Ott PA,Anderson MR,Tary-Lehmann M,Lehmann PV

    更新日期:2005-05-01 00:00:00

  • A thymic nurse cell-specific monoclonal antibody.

    abstract::A thymic epithelial cell line (tsTNC-1) that maintains the ability to selectively bind and internalize immature alpha beta TCRloCD4+CD8+ thymocytes in vitro was used in the development of a monoclonal antibody that is specific to the cell surface of thymic nurse cells (TNCs) in the thymus. The rat monoclonal antibody ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Pezzano M,King KD,Philp DD,Adeyemi A,Gardiner B,Yang J,Samms M,Boto W,Guyden JC

    更新日期:1998-05-01 00:00:00

  • Surface immunoglobulins as targets for anti-immunoglobulin-dependent cell-mediated lysis of B cells.

    abstract::Human K cells are able to lyse human lymphoblastoid B cells in the presence of specific anti-human immunoglobulin isotype antibodies. The human lymphoblastoid B-cell line DAUDI (surface mu- and kappa-chains positive) was lysed in the presence of anti-mu and anti-kappa antisera, or IgG fraction of these antisera, and B...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Caraux J,Weigle WO

    更新日期:1983-03-01 00:00:00

  • Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo.

    abstract::In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Schaefer BC,Schaefer ML,Kappler JW,Marrack P,Kedl RM

    更新日期:2001-12-15 00:00:00

  • HLA class I-restricted and tumor-specific cytotoxic T lymphocytes from metastatic lymph nodes of esophageal cancers.

    abstract::This paper investigates the presence of HLA class I-restricted and tumor-specific cytotoxic T lymphocytes (CTL) in tumor sites of esophageal cancers. Five CTL lines were established from the metastatic lymph nodes or pleural effusion by incubation with interleukin-2 of tumor-infiltrating lymphocytes: cases 1 and 5, HL...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Toh U,Yamana H,Nakao M,Imai Y,Seki N,Takasu H,Kaneshige T,Fujita H,Shirouzu K,Itoh K

    更新日期:1997-05-01 00:00:00

  • Mutations in the alpha 1 domain of a class I gene define residues important for specific allorecognition.

    abstract::Our strategy to use saturation mutagenesis to produce an unbiased collection of major histocompatibility class I mutants has resulted in unpredicted mutant phenotypes. First, we have shown data supporting our earlier work of the Dp20(Y27N) mutant. Allorecognition is altered at the clonal level while no variation in ly...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Murray R,Katoh K,Alexander J,Muller D,Pederson K,Frelinger JA

    更新日期:1990-06-01 00:00:00

  • Viral-restricted cytolytic T lymphocyte recognition of hybrid human-murine class I histocompatibility antigens.

    abstract::Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (alpha 1 and alpha 2) of H-2Kb and the carboxy-terminal domains (alpha 3, transmembrane, and ...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Maziarz RT,Burakoff SJ,Reiss CS

    更新日期:1986-04-15 00:00:00

  • Activation of human peripheral blood-derived monocytes by OK-432 (Streptococcus pyogenes): augmented cytotoxicity and secretion of TNF and synergy with rIFN-gamma.

    abstract::The biological response modifier OK-432 has been shown both to exert the enhancement of several immunological activities and to have a direct anti-tumor effect. The present study examines the immunopotentiating effect of OK-432 on peripheral blood monocytes (PBM) derived from normal humans. Monocyte activation was ass...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Bonavida B,Jewett A

    更新日期:1989-10-15 00:00:00

  • Effects of the methanol extraction residue (MER) tubercle bacillus fraction on the production of antibodies in vitro. III. Consequence of prior sensitization to MER.

    abstract::Mice repeatedly immunized with the methanol extraction residue fraction of tubercle bacilli (MER) in incomplete Freund's adjuvant produced high titers of circulating antibodies against MER, as assessed by the enzyme-linked immunosorbent assay (ELISA) method. Spleen cells derived from these animals failed to respond to...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Halperin D,Reuben C,Ben-Efraim S,Grover N,Weiss DW

    更新日期:1985-05-01 00:00:00

  • Experimental autoimmune encephalomyelitis in intercellular adhesion molecule-1-deficient mice.

    abstract::Intercellular adhesion molecule (ICAM)-1, or CD54, is a member of the immunoglobulin superfamily that binds to lymphocyte function-associated antigen-1 and macrophage-1 antigen. ICAM-1:LFA-1/Mac-1 interaction may be involved in both activation and extravasation of leukocytes. To determine the roles of ICAM-1 in the de...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Samoilova EB,Horton JL,Chen Y

    更新日期:1998-11-25 00:00:00

  • Hormonal regulation of soluble immune response suppressor (SIRS): a possible role of SIRS in the maintenance of pregnancy.

    abstract::This study was conducted to investigate the effects of sex hormones upon the nature of soluble immune response suppressor (SIRS) produced by concanavalin A-stimulated Lyt-2+ T cells. Conventional SIRS affected IgM PFC only. However, SIRS made with progesterone (20-400 ng/ml or Prog-SIRS) suppressed IgM PFC, one-way ML...

    journal_title:Cellular immunology

    pub_type: 杂志文章


    authors: Kita E,Hamuro A,Oku D,Nishikawa F,Yasui K,Emoto M,Katsui N,Kashiba S

    更新日期:1990-10-01 00:00:00