Abstract:
:Peripheral blood leukocytes (PBL), nonadherent lymphocytes, and adherent monocytes separated from freshly isolated blood of 15 dogs were analyzed for their ability to mediate antibody-dependent cell-mediated cytotoxicity (ADCC) in combination with murine anti-tumor monoclonal antibodies (MAbs). Canine monocytes isolated from most donors by adherence to gelatin-fibronectin-coated plastic surface presented high ADCC activity against the canine lymphoma 17-71 tumor cell line in combination with antilymphoma MAbs 231 (IgG2a) and 234-2a (IgG2a). Canine lymphocytes generally showed lower ADCC activity than total PBL or monocytes. Canine PBL effector cells showed high ADCC activity against the human colorectal carcinoma SW948 cell line using the Y-6-specific MAb isotype switch variants 55-2 IgG3, 55-2 IgG1, 55-2 IgG2b, and 55-2 IgG2a. Analysis of the role of murine MAb isotypes on ADCC activity against tumors by canine cells using anti-human tumor class-switch variant MAbs and a panel of anti-canine lymphoma MAbs of different IgG subclass revealed the highest ADCC activity with MAbs of the IgG2a and IgG3 subclasses. IgG2a antilymphoma MAbs were also able to lyse tumor cells in complement-dependent cytotoxicity (CDC) assay. These results suggest the potential value of MAbs of IgG3 and IgG2a subclasses in immunotherapy against canine lymphoma.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Rosales C,Jeglum KA,Obrocka M,Steplewski Zdoi
10.1016/0008-8749(88)90194-3subject
Has Abstractpub_date
1988-09-01 00:00:00pages
420-8issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(88)90194-3journal_volume
115pub_type
杂志文章abstract::We have investigated the ability of interleukin-10 (IL-10) to modulate murine intrathymic T cell differentiation using a fetal thymic organ culture (FTOC) model. Addition of as little as 11 ng of recombinant murine IL-10 (mIL-10) per day produced a significant increase in the proportion and number of gamma delta-TCR+ ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1105
更新日期:1994-04-15 00:00:00
abstract::Tumors harbor a population of cancer stem cells (CSCs) which can drive tumor progression and therapeutical resistance. Nature killer (NK) cells are best known for their ability to directly recognize and kill malignant cells. However, the susceptibility of cancer stem cells to NK cells is not fully understood. Here we ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.11.009
更新日期:2016-02-01 00:00:00
abstract::AK-5 is a spontaneously regressing rat histiocytoma and the tumor regression is mediated by CD8+ NK cells. We have investigated the functional status of effector cells mediating complete remission of the tumor. The effector-target interaction takes place with the help of antibody to AK-5 and the mechanism of lysis inv...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1056
更新日期:1994-03-01 00:00:00
abstract::Human mononuclear cells stimulated with soluble IgG aggregates generated chemiluminescence, a response attributable to monocytes. Some requirements of this reaction were examined by preincubation of the cells with a variety of inhibitors. The protease antagonists TPCK and TLCK, the phospholipase inhibitors quinacrine ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90149-7
更新日期:1984-08-01 00:00:00
abstract::The incubation of murine splenocytes in recombinant interleukin 2 (IL-2) gives rise to both lymphokine-activated killer (LAK) cells capable of lysing fresh tumor cells and cells capable of mediating antibody-dependent cellular cytotoxicity (ADCC) in the presence of anti-H2 allosera. A similarity between these two IL-2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90180-3
更新日期:1988-09-01 00:00:00
abstract::The proapoptotic caspase adaptor protein (PACAP) is involved in cell-cycle regulation and promotes apoptosis. Both MGC29506 and PACAP are isoforms of the MGC29506 gene and are generated by differential splicing of the alternative splice-acceptor. In studying PACAP, we inadvertently constructed the eukaryotic expressio...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.01.006
更新日期:2013-01-01 00:00:00
abstract::IL-5 is a pleiotropic cytokine that promotes eosinophil differentiation and survival. While naïve bronchial epithelial cells (BEC) produce low levels of IL-5, the role of BEC-derived IL-5 in allergic airway inflammation is unknown. We now show that BEC, isolated from mice with OVA-induced allergic airway disease (AAD)...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.04.008
更新日期:2010-01-01 00:00:00
abstract::It is suggested that autologous mixed lymphocyte reaction (AMLR) may play an important role in generating suppressor inducer signals and in down-regulating the immune response following self-major histocompatibility recognition. In the present study, monoclonal antibodies directed at cell surface structures on T4+ cel...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90270-x
更新日期:1987-06-01 00:00:00
abstract::The murine T cell hybridoma line, MBI-1.15, secretes a 17-kDa protein which decreases binding activity of the CD23 molecule for its natural ligand, IgE. This protein, denoted epsilon receptor-modulating protein (epsilon RMP), was previously characterized and shown to be a novel serine protease. The present studies sho...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1036
更新日期:1994-02-01 00:00:00
abstract::In this study the influence of a non-mitogenic anti-CD3 antibody on accessory cell-dependent antigen and mitogen-induced T-lymphocyte proliferation has been investigated. The antibody was found to completely inhibit PHA, Con A, PWM, and tetanus toxoid stimulation, with no effect on the proliferation induced by the cal...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90014-6
更新日期:1986-06-01 00:00:00
abstract::The systemic adoptive transfer of activated T cells, derived from tumor-draining lymph nodes (LNs), mediates the regression of established tumors. In this study, the requirement of cell adhesion molecules, CD11a/CD18 (LFA-1), CD54 (ICAM-1), CD49d/CD29 (VLA-4), and CD106 (VCAM-1), for T cell infiltration into tumors an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1439
更新日期:1999-03-15 00:00:00
abstract::The proliferative response of peripheral blood mononuclear cells (PBMC) in synthetic serum-free media depends on the presence of sufficient amounts of transferrin (Tf). In the present communication we show that the reduction of Tf concentration in culture media results in a decreased proliferation, whereas lymphokine ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90038-d
更新日期:1991-02-01 00:00:00
abstract::We investigated the influence of Panagen DNA preparations on laboratory animals and IFN-induced human dendritic cells, as well as analyzed the data from a phase II clinical trial in the therapy of breast cancer. It was shown that this treatment resulted in increased number of CD8+/perforin+ T cells in peripheral lymph...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.04.004
更新日期:2012-03-01 00:00:00
abstract::The mechanism underlying the apparent differences in the capacity of murine and human class I MHC molecules to function as signal transducing structures in T cells was examined. Cross-linking murine class I MHC molecules on splenic T cells did not stimulate an increase in intracellular calcium ([Ca2+]i) and failed to ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90254-m
更新日期:1992-10-15 00:00:00
abstract::It has been reported that granulocyte/macrophage colony-stimulating factor (GM-CSF), one of the hemopoietic growth factors which regulates the function of phagocytic cells, is a potent activator of cultured macrophages and induces antimicrobial activities as well as differentiation of precursor cells. In this study, w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.1074
更新日期:1997-02-25 00:00:00
abstract::To determine the relative contributions of DC subsets in the development of protective immunity to Listeria monocytogenes we examined the relationship between maturation, bacterial burden, and T cell priming capacity of four well characterized subsets of splenic DC following infection with Lm. CD8α(+), CD4(+), and CD8...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.03.001
更新日期:2011-01-01 00:00:00
abstract::Murine fibrosarcomas produce a factor that activates suppressor cells to inhibit expression of delayed-type hypersensitivity (DTH) responses to dinitrochlorobenzene (DNCB). This tumor-derived suppressor factor (TDSF) was partially purified by preparative isoelectric focusing of spent medium and 3 M KCl extracts of cul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90384-3
更新日期:1985-06-01 00:00:00
abstract::We examined the effects of a rare sugar, D-allose, which is 6-carbon monosaccharide, on endocytosis and T cell stimulation by dendritic cells (DCs). The endocytosis of BCG-anti-BCG immune complexes by DCs markedly decreased in D-allose-containing medium. Co-culture with T cells (mixed leukocyte reaction, MLR) of DCs, ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.06.015
更新日期:2011-01-01 00:00:00
abstract::Tegumentary leishmaniasis (TL) constitutes a major public health problem with significant morbidity worldwide. Synthetic peptide-based vaccines are attractive candidates to protect against leishmaniasis, since T cell-specific epitopes can be delivery to antigen-presenting cells, leading to the generation of a Th1 cell...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2016.12.008
更新日期:2017-03-01 00:00:00
abstract::The effects of LTs are mediated by GPCRs: cysLTs interact with CYSLTR1, CYSLTR2, or GPR17, and LTB4 acts via BLT1R or BLT2R. Data relating to the presence of these receptors in mature tissue mast cells are not entirely known. By confocal microscopy with image analyses and flow cytometry, we established that native rat...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.04.010
更新日期:2017-07-01 00:00:00
abstract::We have previously demonstrated that natural killer (NK) cells are lysosome-rich and stain more intensely with lysosomotropic agents such as neutral red and quinacrine (Qu) than do non-NK cells. In this study we combined the quantitation of Qu staining with surface marker staining to define subpopulations of NK cells....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90158-x
更新日期:1988-08-01 00:00:00
abstract::A majority (42/62) of TCR2+ interleukin 2-dependent human T lymphocyte clones was found concordantly to express not only the CD28 co-receptor structure at the cell surface but also its ligand B7. Interactions between CD28 and B7 can have important consequences for T cell activation, particularly in providing "signal 2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1238
更新日期:1993-10-15 00:00:00
abstract::A single nucleotide A49G polymorphism (SNP) of CTLA-4 has been linked and associated with the autoimmune thyroid diseases (AITD) and thyroid autoantibody secretion. We have explored the functional mechanisms of CTLA-4 by means of recombinant human CTLA-4 expressed on transfected Jurkat T cells. Analysis of CTLA-4 tran...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/s0008-8749(02)00018-7
更新日期:2002-02-01 00:00:00
abstract::Mice carrying a rearranged TCR Vbeta 8.2 transgene express the Vbeta protein on the vast majority of peripheral T-cells. The bone marrow and peripheral blood, as well as other lymphoid organs of both untreated animals and animals depleted of T-cells by neonatal thymectomy and/or injection from birth of monoclonal anti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1168
更新日期:1997-10-10 00:00:00
abstract::Caused mainly by Candida albicans, oropharyngeal candidiasis is the most common oral complication associated with HIV disease worldwide. Host defenses against C. albicans essentially fall into two categories: specific immune mechanisms and local oral mucosal epithelial cell defenses. Since oral mucosa is the first lin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.11.008
更新日期:2012-11-01 00:00:00
abstract::Interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) are major proinflammatory cytokines inducing the synthesis and release of many inflammatory mediators. They are involved in immune regulation, autoimmune diseases, and inflammation. Acanthoic acid, (-)-pimara-9(11),15-dien-19-oic acid, is a pimaradiene d...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0154
更新日期:1996-06-15 00:00:00
abstract::The effects of oral administration of ovalbumin (OVA) on acute OVA-induced arthritis (OIA) in rats, which is mediated by Arthus reaction to the antigen in the joint space, were investigated. The oral administration of OVA before immunization with OVA significantly suppressed the development of acute OIA in a dose-depe...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.1049
更新日期:1997-01-10 00:00:00
abstract::Peroxynitrite (ONOO(-)) has been vastly implicated in mutagenesis and cancer development. Present study probes the antigenicity of peroxynitrite damaged DNA (ONOO(-)-DNA) in cancer patients. Purified human placental DNA was damaged by the synergistic action of sodium nitroprusside (SNP) and Pyrogallol for 3h at 37 deg...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.08.002
更新日期:2009-01-01 00:00:00
abstract::Eighty-seven murine monoclonal antibodies (MAb) produced against human tumors of various origins and representing six different immunoglobulin classes were tested for antitumor reactivity in antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) assays. Mouse splenocytes, thio...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90068-1
更新日期:1985-04-15 00:00:00
abstract::Eicosanoids are important mediators of inflammation, but also play a role in regulation of lymphocyte function. In this study we have examined the function of both prostaglandins (PGs) and leukotrienes in regulating the release of a set of cytokines produced by T cells from mice primed with the contact-sensitising age...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1157
更新日期:1993-07-01 00:00:00