Abstract:
:We previously showed that DT-5461a, a lipid A analog, has antitumor activity and relatively low toxicity in animals. To clarify its immunostimulatory effect we have studied the cytokine inducibility of DT-5461a and the mechanism involved in the cytokine expression in macrophages. While the activity is lower than that of LPS, DT-5461a enhanced production of TNF-alpha and GM-CSF in the murine macrophage cell line J774.1 in a dose-dependent fashion. Simultaneous addition of IL-4 inhibited DT-5461a and LPS-induced TNF-alpha production in a similar manner. Expression of cytokine mRNAs including TNF-alpha, IL-1 beta, IL-6, and GM-CSF was also enhanced by the treatment with DT-5461a. Treatment with cycloheximide indicated that the expression of TNF-alpha mRNA does not require new protein synthesis. Actinomycin D chase experiments revealed that cytokine mRNAs have rather long half-lives and we could not find significant changes in mRNA stability of cytokines in the J774.1 cells. The binding activity of a nuclear transcription factor NF-kappa B was enhanced by treatment with DT-5461a, suggesting enhanced transcription of cytokine genes. Specific binding of [3H]LPS to the cells was significantly inhibited by the addition of DT-5461a. These results indicate that DT-5461a enhances production of a variety of cytokines in macrophages through the LPS receptor sites and that this cytokine induction may occur primarily via transcriptional enhancement. These immunostimulatory effects may provide evidence for the mechanisms of the antitumor effect of this lipid A analog.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Akahane K,Someya K,Tsutomi Y,Akimoto T,Tohgo Adoi
10.1006/cimm.1994.1100subject
Has Abstractpub_date
1994-04-15 00:00:00pages
42-52issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(84)71100-2journal_volume
155pub_type
杂志文章abstract::Nickel (Ni) can cause delayed-type hypersensitivity reactions, which are thought to be mediated by the accumulation of T cells into inflamed skin. Accumulated T cells at the developmental stages in metal allergy are poorly characterized because a suitable animal model has not been established. To investigate the accum...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.07.010
更新日期:2013-07-01 00:00:00
abstract::Avirulent clones of Leishmania braziliensis were produced by treatment of parental parasite stock with the mutagen N-methyl-N'-methyl-N'-nitro-N-nitrosoguanidine and selection for growth at 19 degrees C. These clones have a clear preference for infection and growth in vitro at 19 degrees C rather than the normal 28 de...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90081-4
更新日期:1985-08-01 00:00:00
abstract::We have analyzed the effect of cyclosporin-A (CsA) on the proliferative and cytotoxic responses induced by mixed-lymphocyte cultures (MLC-s) on low and high density T lymphocytes. Allogeneic stimulation had a different impact on the two subsets. Proliferative and cytotoxic responses were inversely correlated; i.e., hi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90100-0
更新日期:1986-12-01 00:00:00
abstract::Mycobacterium bovis BCG and its subcellular components (bacterial extract, culture filtrate, purified protein derivative, and muramyl dipeptide MDP) are potent in vitro IL-6 inducers in spleen cell cultures from uninfected and BCG-infected BALB/c mice. Both plastic adherent and nonadherent spleen cells are capable of ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90071-i
更新日期:1991-10-01 00:00:00
abstract::Strong delayed-type hypersensitivity (DTH) to Babesia microti was elicited when intraerythrocytic parasites (IEP) were inoculated subcutaneously into the flank of normal mice 6 to 14 days before challenge in the ipsilateral footpad with 10(8) IEP. Intraperitoneal or intravenous administration of antigen did not sensit...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90289-3
更新日期:1986-04-01 00:00:00
abstract::We have examined noninduced cytotoxicity of mouse gut associated and peripheral lymphoid tissues for a wide variety of syngeneic as well as allogeneic cell lines and lymphoblasts. Lymphoid cells from Peyer's patches were found to lyse these targets in a 3-hr chromium release assay whereas lymphoid cells from intestina...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90158-9
更新日期:1986-09-01 00:00:00
abstract::The Interferon-inducible gene IFI16, a member of the HIN200 family, is activated by oxidative stress and cell density, in addition to Interferons, and it is implicated in the regulation of endothelial cell proliferation and vessel formation in vitro. We have previously shown that IFI16 is required for proinflammatory ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.02.007
更新日期:2009-01-01 00:00:00
abstract::We recently reported that a cloned murine cell line with "natural killer (NK)-like" cytolytic function and prominent cytoplasmic granules also expressed large numbers of plasma membrane receptors (Fc epsilon R) which bound mouse immunoglobulin E (IgE) with high affinity (S.J. Galli et al., 1982, Nature (London) 298, 2...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90353-3
更新日期:1985-11-01 00:00:00
abstract::Dipeptidyl peptidase IV (DP IV, CD26), known as an activation marker of T lymphocytes, is a proline-specific protease thought to be involved in the regulation of the immune response. The physiological role of dipeptidyl peptidase IV in the immune system and the molecular events of lymphocyte activation mediated by thi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0134
更新日期:1996-05-25 00:00:00
abstract::We have studied the antibody-induced effect of cross-linking the major histocompatibility complex class I (MHC-I) in human T leukemic cells (Jurkat) and human B lymphoma cells (Solubo, Burkitts lymphoma) on intracellular [Ca2+]i levels. The increase in [Ca2+]i after MHC-I cross-linking in Jurkat cells and Solubo cells...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0281
更新日期:1996-11-01 00:00:00
abstract::Experimental autoimmune thyroiditis is induced in mice by immunization with thyroglobulin emulsified in Freund's complete adjuvant. The disease is characterized both by thyroid infiltration with mononuclear cells and by circulating thyroglobulin antibodies. The magnitude of the thyroid infiltration and the titer of th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90302-9
更新日期:1987-10-01 00:00:00
abstract::OVA-specific T cells were immortalized by infection with radiation leukemia virus (RadLV). Some clones derived from such population were shown to exhibit helper activity. We then tested clones without such function and found among them some that secreted macrophage migration inhibition factor (MIF) and leukocyte migra...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90328-x
更新日期:1986-10-01 00:00:00
abstract::Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is caused by mutations in the AIRE gene. Murine studies suggest that AIRE controls thymic expression of tissue-restricted antigens, its absence allowing nonselected autoreactive cells to escape. We tested this in humans using the TCRβ CDR3 length ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.03.005
更新日期:2015-06-01 00:00:00
abstract::Semaphorins are important regulators of peripheral T and B-cell mediated immune responses in mice and humans. Modulatory roles of semaphorins in T cell development are also being characterized. We carefully analyzed the gene expression and protein levels of semaphorins 4A, 4D, and 7A at various developmental stages of...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.03.006
更新日期:2013-02-01 00:00:00
abstract::We have performed immunoperoxidase staining on cryostat tissue sections and immunofluorescence analysis on cell suspensions to identify cells expressing the alpha/beta T cell antigen receptor during ontogeny and adult life in chickens. We used the mouse monoclonal antibody, TCR2, which was previously shown to recogniz...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90079-7
更新日期:1990-01-01 00:00:00
abstract::Accessory cell (A-cell) function in a Con A response was analyzed. Irradiated P388D1 cells efficiently induced a proliferative response to Con A of T cells purified from spleen cells, whereas paraformaldehyde-fixed P388D1 cells failed to serve as A cells. Although IL-1 containing culture supernatant (SN) of a macropha...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90169-9
更新日期:1987-05-01 00:00:00
abstract::Mature thymocytes are usually defined and separated from other less mature thymocytes on the basis of their mutually exclusive expression of either CD4 or CD8. However, such murine "single positives" include a subpopulation of immature cells with properties resembling CD4- CD8- thymocytes or CD4+ CD8+ cortical blasts....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90042-1
更新日期:1988-05-01 00:00:00
abstract::Dendritic cell (DC)-based immunotherapy has promising for treatment of non-small cell lung cancer (NSCLC). Melanoma-associated antigen 3 (MAGE-A3) is a tumor-specific antigen and expressed in approximately 35-40% of NSCLC tissues. Calreticulin (CALR) is a protein chaperone and can enhance DC maturation and antigen pre...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.12.003
更新日期:2016-02-01 00:00:00
abstract::Like the thymus, the bursa of Fabricius is a site of massive lymphopoiesis accompanied by cell death in vivo. In the present study we have, therefore, examined whether chicken bursa and thymus cells exhibit apoptosis. Bursa and thymus cells from SC chickens, 4-10 weeks of age, were incubated for 8-24 hr with various r...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1058
更新日期:1993-03-01 00:00:00
abstract::The glycobiology of the immune response is a topic that has garnered increased attention due to a number of key discoveries surrounding IgG function, the specificity of some broadly neutralizing anti-HIV antibodies, cancer immunoregulation by galectin molecules and others. This review is the opening article in a Speci...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2018.04.010
更新日期:2018-11-01 00:00:00
abstract::Antigen-independent adhesion of CD4+ T lymphocytes to Epstein-Barr virus transformed B cells is mainly mediated by LFA-1 (CD11a/CD18) and CD2 molecules. Low-affinity binding of resting T cells can be transiently upregulated by cross-linking of CD3-TCR (T cell receptor) complexes. This inside-out signaling influences i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1211
更新日期:1993-09-01 00:00:00
abstract::Mouse thyroglobulin (MTg)-sensitized spleen cells activated in vitro with MTg induced experimental autoimmune thyroiditis (EAT) in which the thyroid infiltrate consists primarily of mononuclear cells (MNC) (lymphocytic EAT). MTg-sensitized spleen cells cultured with MTg together with anti-IL2R antibody induce a granul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0208
更新日期:1996-08-25 00:00:00
abstract::During the gestational cycle the placental tissue does not express class II MHC antigens and whether this phenomenon is important to fetal survival has not yet been evoked. It has been reported that class II antigen expression precedes renal and cardiac graft rejection, which may also be the case in fetal abortion. In...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90039-t
更新日期:1990-07-01 00:00:00
abstract::The effect of the addition of dialyzable leukocyte extract (DLE)(transfer factor) to tuberculostatic drugs in the treatment of superinfected fistulating tuberculosis of bones and joints was evaluated in a controlled study. Eleven patients whose disease had persisted for a mean of 20 +/- 4.8 years and had proved to be ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90091-1
更新日期:1984-03-01 00:00:00
abstract::The regulatory mechanism of guinea pig lymphokines was investigated in regard to differentiation of myeloid cells to macrophages. The Ml-cell line, established from a myeloid leukemia of an SL-strain mouse, was induced to differentiate in vitro into mature macrophages possessing Fc receptors and the ability to phagocy...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90355-6
更新日期:1983-02-15 00:00:00
abstract::The loss of thymic function with age may be due to diminished numbers of T-cell progenitors and the loss of critical mediators within the thymic microenvironment. To assess the molecular changes associated with this loss, we examined transcriptomes of progressively aging mouse thymi, of different sexes and on caloric-...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.03.008
更新日期:2007-01-01 00:00:00
abstract::Aging diminishes the amount of phosphotyrosine in the CD3zeta chains of resting and activated mouse CD4 T cells by about threefold and might therefore be expected to a corresponding decline in Zap-70 association with CD3zeta and in Zap-70 kinase function in CD3zeta complexes. We show here that aging leads, unexpectedl...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1394
更新日期:1998-12-15 00:00:00
abstract::Unique dysregulated expression patterns of microRNAs (miRNAs) have been reported in many disease conditions including autoimmune diseases such as systemic lupus erythematosus (SLE). We have recently reported that miRNAs are differentially expressed not only between autoimmune and control mice, but also between male an...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2015.01.004
更新日期:2015-04-01 00:00:00
abstract::The internalization of surface immunoglobulin (Ig) by B lymphocytes is the first step in the antigen-presenting function performed by these cells. Mature B cells coexpress on their surface IgM and IgD. At this time, there is controversy over whether these two isotypes serve different functions in the antigen-presentin...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90305-5
更新日期:1989-10-15 00:00:00
abstract::Cyclosporin A (CsA) was tested for its modulatory effects on the mIgM-mediated signaling of G0*-associated increases in class II MHC expression, G1-related RNA synthesis, and S phase-related DNA synthesis in human B cells. While CsA at concentrations as low as 10-100 ng/ml could completely ablate anti-IgM-induced DNA ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90213-9
更新日期:1992-04-01 00:00:00