Abstract:
:Nonenzymatic glycation of amino groups of DNA bases by reducing sugars can generate advanced glycation end products (AGEs). Cellular formation of AGEs under normal physiology is continuously scanned and removed by efficient system in the cells. However, excess formation and accumulation of AGEs may be cause or consequence of some human diseases. Mammalian DNA incubated with d-glucose for 28 days at 37°C showed structural changes in DNA as confirmed by UV, fluorescence, CD, melting temperature, S1 nuclease sensitivity and gel electrophoresis. Formation of DNA-AGE was confirmed by HPLC and LC-MS. Enzyme immunoassay and electrophoretic mobility shift assay of autoantibodies in type 2 diabetes patients' sera with disease duration of 5-15 years exhibited significantly high binding with DNA-AGE as compared to patients with 1-5 years of disease duration. Autoantibodies against aberrant DNA-AGE may be important in the assessment of initiation/progression of secondary complications in type 2 diabetes mellitus patients.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Ashraf JM,Arfat MY,Arif Z,Ahmad J,Moinuddin,Alam Kdoi
10.1016/j.cellimm.2014.12.007subject
Has Abstractpub_date
2015-02-01 00:00:00pages
74-9issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(14)00184-1journal_volume
293pub_type
杂志文章abstract::The experiments in this manuscript confirm and extend our previous finding that IL4 has minimal enhancing activity on B cell activation stimulated by anti-Ig-dextran conjugates. The absence of significant IL4-mediated enhancement is seen also under conditions which are limiting for optimal B cell proliferation. Thus, ...
journal_title:Cellular immunology
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abstract::Transduction with recombinant, replication-defective adenoviral (rAd) vectors encoding a transgene is an efficient method for gene transfer into human dendritic cells (DCs). Livin is a good candidate for cancer immunotherapy since it is overexpressed in most common human cancers, poorly expressed in most normal adult ...
journal_title:Cellular immunology
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
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abstract::Mouse thyroglobulin (MTg)-sensitized spleen cells activated in vitro with MTg induced experimental autoimmune thyroiditis (EAT) in which the thyroid infiltrate consists primarily of mononuclear cells (MNC) (lymphocytic EAT). MTg-sensitized spleen cells cultured with MTg together with anti-IL2R antibody induce a granul...
journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1209
更新日期:1993-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2014.02.007
更新日期:2014-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.05.005
更新日期:2013-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90326-6
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journal_title:Cellular immunology
pub_type: 杂志文章,评审
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更新日期:2010-01-01 00:00:00
abstract::IL-2 was previously shown to induce cytotoxic effectors with a broad spectrum of target specificities in thymus and spleen cell cultures. This study was designed to show whether T cells activated by H-2 allogeneic cells in MLC or by syngeneic tumor cells in MLTC are also potential targets for these cytotoxic effectors...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90124-x
更新日期:1989-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1116
更新日期:1993-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.07.009
更新日期:2012-07-01 00:00:00
abstract::Interferon-gamma is a critical factor in the activation of several mononuclear phagocyte effector and immunoregulatory properties. However, it remains uncertain if IFN-gamma is capable of concurrent activation of both functions in the same cell population. Plastic adherent mononuclear cells (80-98% MN by cytochemical ...
journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1986-06-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.07.003
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2015.12.003
更新日期:2016-02-01 00:00:00
abstract::Murine peritoneal macrophages (M phi), activated in vivo or in vitro, remarkably inhibited the uptake of thymidine by a lens epithelial cell line, while resident M phi, or M phi induced by thioglycollate, exhibited much lower or no cytostatic capacity. The target cells were partially protected from the cytostatic acti...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90222-3
更新日期:1984-01-01 00:00:00
abstract::Toll-like receptors are a family of pattern-recognition receptors that contribute to the innate immune response. Toll-like receptor 3 (TLR3) signals in response to foreign, endogenous and synthetic ligands including viral dsRNA, bacterial RNA, mitochondrial RNA, endogenous necrotic cell mRNA and the synthetic dsRNA an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.10.002
更新日期:2007-08-01 00:00:00