Abstract：
:Soluble suppressor factors in the sera of cancer patients inhibit lectin-stimulated lymphocyte proliferation. These factors, derived from human material, preclude easy corroboration by other investigators. To gain a general understanding of soluble suppressor factors and to avoid the necessary restrictions of human experimentation, an animal model was devised. Sprague-Dawley rats were injected ip with the Walker 256 carcinoma. The resultant ascites proved to be a stable, reproducible source of soluble suppressor factors. Ascites inhibited phytohemagglutinin (PHA)-induced blastogenesis of normal splenocytes by 98%. The possibility of a toxic effect was eliminated by vital staining of splenocytes and by examination in a specific lymphotoxin assay. Suppressor activity persisted after heating at 100 degrees C for 40 min. Extraction by lipid solvents revealed that the bulk of suppressor activity resides in the lipid phase. The active fraction of heat-treated ascites passed through an Amicon PM-10 filter. Thin-layer chromatography revealed the presence of prostaglandins E2 and F2 alpha. Tissue culture supernatants from short-term cultures derived from tumor-bearing animals revealed suppressor activity from thymus, spleen, and liver cultures (97, 91, and 71%, respectively). No suppressor activity was detected in cultures of cancer cells. This study has demonstrated in this animal model that prostaglandins play a major role in suppression of lectin-induced blastogenesis. All suppressor factors appear to be host derived. An understanding of the mechanism of release of these suppressor substances may open new avenues in the immunotherapy of cancer.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Wile AG,Hensen D,Nahabedian M,Ibsen KH,Granger GAdoi
10.1016/0008-8749(84)90389-7subject
Has Abstractpub_date
1984-07-01 00:00:00pages
347-53issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(84)90389-7journal_volume
86pub_type
杂志文章abstract：:Cultures of gradient-purified human peripheral blood mononuclear cells (PBMC) have been employed to examine the effects of three bacteria-derived human leukocyte interferon subtypes on certain aspects of in vitro immune responses. The addition of highly purified IFN-alpha 1, -alpha 2, -alpha 2/alpha 1 to PMBC cultures...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90161-2
更新日期：1983-12-01 00:00:00
abstract：:Because of the massive cytokine response elicited by superantigen exposure, it has been suggested that superantigens may act as adjuvants to boost conventional antigen responses. However, most previous studies have shown that in vivo exposure to superantigen suppressed subsequent T cell responses. Here we analyzed the...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.1077
更新日期：1997-03-15 00:00:00
abstract：:The mechanism underlying the apparent differences in the capacity of murine and human class I MHC molecules to function as signal transducing structures in T cells was examined. Cross-linking murine class I MHC molecules on splenic T cells did not stimulate an increase in intracellular calcium ([Ca2+]i) and failed to ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90254-m
更新日期：1992-10-15 00:00:00
abstract：:The transfer of B cells from mice immunized with Type III pneumococcal polysaccharide (SSS-III) results in the activation of suppressor and amplifier T cells that control the magnitude of the antibody response in recipient mice, immunized subsequently with SSS-III. Prior treatment of transferred B cells with an excess...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90368-5
更新日期：1985-12-01 00:00:00
abstract：:The adoptive transfer of tumor-reactive T lymphocytes has recently been demonstrated to be an effective means for mediating the regression of experimental intracranial fibrosarcomas. In this study, mice bearing syngeneic intracranial GL261 gliomas were cured by the combination of sublethal whole body irradiation follo...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1140
更新日期：1997-06-15 00:00:00
abstract：:The nonspecific suppression of immunological responses that is generated within host popliteal lymph nodes upon exposure to syngeneic normal spleen cells has been examined. The suppression, which had previously been described as being capable of preventing initiation of cytotoxic T lymphocytes (CTLs) to hapten-altered...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90069-4
更新日期：1987-03-01 00:00:00
abstract：:Seeking common abnormalities in mice genetically predisposed to lupus-like autoimmune disease, we investigated (1) the ontogeny of Ia antigens (I-A/I-E) on the surfaces of resident peritoneal macrophages (rpM phi) of lupus and normal mice, (2) spontaneous and lectin-induced in vitro production of M phi-stimulating fac...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90133-3
更新日期：1984-08-01 00:00:00
abstract：:Immunity to Schistosoma mansoni is regulated by an auto-anti-idiotypic network. The T cells in this network interact through soluble regulatory factors. These studies describe a soluble suppressor-inducer factor (TsiF) derived from TCR+ suppressor-inducer clone Q37. Q37 is the fusion product of CD4+ T cells derived fr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1144
更新日期：1993-06-01 00:00:00
abstract：:Dendritic cells initiate the immune response by presenting antigen in the context of varying levels of costimulation. The maturation state of the dendritic cell determines the quantity and quality (Th1, Th2) of the subsequent T cell response. Members of the NF-kappaB family of transcription factors have previously bee...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/s0008-8749(03)00114-x
更新日期：2003-04-01 00:00:00
abstract：:To investigate the process of tolerance induction we have developed an in vivo model using TCR beta-chain transgenic mice tolerized with the superantigen staphylococcal enterotoxin B. We have previously demonstrated that tolerized peripheral T cells were anergic when stimulated in vitro with immunogenic peptides, supe...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1177
更新日期：1994-07-01 00:00:00
abstract：:Avirulent clones of Leishmania braziliensis were produced by treatment of parental parasite stock with the mutagen N-methyl-N'-methyl-N'-nitro-N-nitrosoguanidine and selection for growth at 19 degrees C. These clones have a clear preference for infection and growth in vitro at 19 degrees C rather than the normal 28 de...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90081-4
更新日期：1985-08-01 00:00:00
abstract：:Senescence is characterized by several alterations in the immune system. Such modifications can be found in lymphoid organs as well as in the cellular components of the immune system. Several reports have suggested that immune dysfunction can affect both T and B cells, but T cells have been shown to be more susceptibl...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.01.010
更新日期：2009-01-01 00:00:00
abstract：:Visceral leishmaniasis (VL) is a fatal disease when acute and untreated. The treatment against this disease is long and presents toxicity and/or high costs. Moreover, parasite resistance has been increasing. Therefore, alternative control measures to avoid the spread of disease should be considered. It is accepted tha...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.05.009
更新日期：2018-09-01 00:00:00
abstract：:Although it is well known that the CD3/T-cell receptor (TCR) complex modulates from the surface of T cells upon exposure to monoclonal antibodies (mAb) directed against it, the fate of bound mAb has not been yet elucidated. We therefore perform direct binding experiments of 125I-labeled mAb against CD3 or TCR to inves...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90209-2
更新日期：1988-10-01 00:00:00
abstract：:We have performed immunoperoxidase staining on cryostat tissue sections and immunofluorescence analysis on cell suspensions to identify cells expressing the alpha/beta T cell antigen receptor during ontogeny and adult life in chickens. We used the mouse monoclonal antibody, TCR2, which was previously shown to recogniz...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90079-7
更新日期：1990-01-01 00:00:00
abstract：:The activation by concanavalin A (Con A) of murine lymphocytes derived from aged animals is impaired, as demonstrated by lower IL-2 synthesis, display of fewer IL-2 receptors, and less [3H]thymidine incorporation relative to similarly treated cells from young animals. The ability of the phorbol ester, phorbol 12-myris...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90250-x
更新日期：1988-06-01 00:00:00
abstract：:The mechanism by which purinergic agonists modulate murine T-lymphocyte activation and proliferation was investigated. Adenosine and other compounds such as ATP and 2-chloroadenosine (ClAdo) were found to block T-cell mitogenesis induced by concanavalin A (Con A) in a dose-dependent fashion. The nonmetabolizable adeno...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90199-1
更新日期：1986-08-01 00:00:00
abstract：:Immunization with synthetic mRNA encoding tumor-associated antigens is an emerging vaccine strategy for the treatment of cancer. In order to prevent mRNA degradation, promote antigen-presenting cells antigen presentation, and induce an anti-tumor immune response, we investigated the nasal administration of mRNA vaccin...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2020.104143
更新日期：2020-08-01 00:00:00
abstract：:Mutations in the X-linked inhibitor of apoptosis (XIAP) have recently been identified in patients with the rare genetic disease, X-linked lymphoproliferative syndrome (XLP), which was previously thought to be solely attributable to mutations in a distinct gene, SAP. To further understand the roles of these two factors...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2009.05.017
更新日期：2009-01-01 00:00:00
abstract：:The experiments in this manuscript confirm and extend our previous finding that IL4 has minimal enhancing activity on B cell activation stimulated by anti-Ig-dextran conjugates. The absence of significant IL4-mediated enhancement is seen also under conditions which are limiting for optimal B cell proliferation. Thus, ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90275-v
更新日期：1990-10-15 00:00:00
abstract：:Macrophages can be divided into two groups as M1 and M2 phenotype. Our results and other groups revealed that IFN-γ can up-regulate the IDO expression and differentiate THP-1 cells to M1 phenotype. Therefore we hypothesized that IDO may play potential roles in macrophage differentiation. Interesting, our results indic...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2014.02.005
更新日期：2014-05-01 00:00:00
abstract：:Tumors harbor a population of cancer stem cells (CSCs) which can drive tumor progression and therapeutical resistance. Nature killer (NK) cells are best known for their ability to directly recognize and kill malignant cells. However, the susceptibility of cancer stem cells to NK cells is not fully understood. Here we ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.11.009
更新日期：2016-02-01 00:00:00
abstract：:We have addressed the differential regulatory properties that IFN-gamma and IL-4 exert on macrophage (M phi) subpopulations. For this purpose, Thyoglicolate-, Peptone-, and Con A-elicited M phi, as well as bone marrow-derived M phi and P388D1 cells, were cultured in the presence of either IFN-gamma or IL-4. The expres...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90347-e
更新日期：1991-04-15 00:00:00
abstract：:Conalbumin (CA)-specific type 2 helper T cell (Th2) clone, D10G4.1 (D10) produces IL4 when stimulated with varying doses of TNP-CA in the presence of mitomycin C-treated C3H spleen cells or purified B cells as antigen-presenting cells (APC). The production of IL4 was assessed by bioassay and by expression of IL4 mRNA....
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90333-k
更新日期：1992-12-01 00:00:00
abstract：:We have reported that Burkitt lymphomas (BL) that arise in HLA-A11 positive individuals are resistant to lysis by HLA-A11-specific and HLA-A11-restricted CTLs(10,11). Here we show that this phenomenon can be explained by a selective loss of the HLA-A11 polypeptide. The HLA-A11 negative phenotype is due to a regulatory...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90207-4
更新日期：1989-05-01 00:00:00
abstract：:Aging is characterized by significant immune remodeling at both cellular and molecular levels, also known as immunosenescence. Older adults often manifest a chronic low-grade inflammatory phenotype. These age-related immune system changes have increasingly been recognized not only to lead to immune functional decline ...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2019.104024
更新日期：2020-02-01 00:00:00
abstract：:The antigenic phenotype of individual PHA-induced T lymphocyte colonies was studied with a direct immunofluorescence technique using fluorescein-labeled anti-Leu-2a and anti-Leu-3a antibodies. Of the colonies grown from mononuclear peripheral blood cells 85% were Leu-3a+ (inducer/helper phenotype), 12% were Leu-2a+ (s...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90235-6
更新日期：1983-10-15 00:00:00
abstract：:In previous studies, the murine SaI (A/J derived, KkDd) sarcoma was transfected with the allogeneic MHC class I H-2Kb gene, and expressed high levels of H-2Kb antigen. Contrary to expectations, the tumor cells expressing the alloantigen (SKB3.1M tumor cells) were not rejected by autologous A/J mice. Because these resu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90319-7
更新日期：1991-05-01 00:00:00
abstract：:Tiazofurin treatment of K562 leukemia cells in vitro depletes the metabolites of the guanylate biosynthetic pathway, inducing erythroid differentiation, that, in turn, alters the phenotypic profile. As a consequence, K562 cells possibly modify their interaction with immune cells. Here we describe the binding and killi...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1147
更新日期：1995-08-01 00:00:00
abstract：:The systemic adoptive transfer of activated T cells, derived from tumor-draining lymph nodes (LNs), mediates the regression of established tumors. In this study, the requirement of cell adhesion molecules, CD11a/CD18 (LFA-1), CD54 (ICAM-1), CD49d/CD29 (VLA-4), and CD106 (VCAM-1), for T cell infiltration into tumors an...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1998.1439
更新日期：1999-03-15 00:00:00
